Verification for the presence of inhibitory substances in poultry meat after the consumption of the feed mixture supplemented with fermented feed
DOI:
https://doi.org/10.5219/682Keywords:
meat, feed, residues, screeningAbstract
The European Union has an obligation to ensure that feed produced in the European Union is safe for animals and also humans by ensuring food of animal origin is safe and wholesome. An increasing demand for safe, wholesome and nutritious animal products has led to the search for alternative substances in animal feed. Fermented feed has gained a lot of popularity in many animal diets today. They meet the demand for animal nutrition due to the formation of target substances with the desired properties. As some of them are attracting attention as potential antimicrobial agents that inhibit the growth of certain microorganisms, and the products of animal origin are controlled for the presence of residues of inhibitory (antimicrobial) substances, the aim of this work was to verify the presence of inhibitory substances in poultry meat (muscle, heart, liver, kidneys of broiler chickens) after the consumption of the feed mixture with addition of fermented feed (wheat bran fermented with the strain Umbelopsis isabellina CCF 2412) in a dose of 10 % of the total amount of the feed. The detection of residues was performed by two approved microbiological screening methods, the screening test for the detection of antibiotic residues (STAR) and the Premi®Test. Both methods detected the positive results and pre-identified the presence of residues of the inhibitory substances not only in the meat of broiler chickens but also in the investigated fermented feed. Due to the antimicrobial potential of the fermented feed and the possible presence of the false-positive results, each positive result must be confirmed by a confirmatory analysis.
Downloads
References
Bobko, M., Haščík, P., Mellen, M., Bobková, A., Tkáčová, J., Czako, P., Pavelková, A., Trembecká, L. 2016. Effect of different phytogenic additives on oxidation stability of chicken meat. Potravinarstvo, vol. 10, no. 1, p. 164-169. https://doi.org/10.5219/567
Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. OJ L 221, 17.8.2002, p. 8-36.
Commission Regulation (EC) No. 167/2008 of 22 February 2008 concerning a new authorization of coccidiostats as feed additive for ten years. OJ L50, 23.2.2008, p. 1-3.
Council Directive 96/23/EC of 29 April 1996 on measures to monitor certain substances and residues thereof in live animals and animal products and repealing Directives 85/358/EEC and 86/469/EEC and Decisions 89/187/EEC and 91/664/EEC. OJ L 125, 23.5.1996, p. 10-32.
Čertík, M., Klempová, T. 2016. Functional cereal-derived biomaterials enriched with lipophilic compounds prepared by fungal solid-state fermentations. Materials Science Forum, vol. 851, p. 8-13. https://doi.org/10.4028/www.scientific.net/MSF.851.8
Čertík, M., Adamechová, Z., Guothová, L. 2013a. Simultaneous enrichment of cereals with polyunsaturated fatty acids and pigments by fungal solid state fermentations. Journal of Biotechnology, vol. 168, p. 130-134. https://doi.org/10.1016/j.jbiotec.2013.03.016 PMid:23583333
Čertík, M., Klempová, T., Guothová, L., Mihálik, D., Kraic, J. 2013b. Biotechnology for the functional improvement of cereal-based materials enriched with polyunsaturated fatty acids and pigments. European Journal of Lipid Science and Technology, vol. 115, no. 11, p. 1247-1256. https://doi.org/10.1002/ejlt.201300092
Cheng, G., Hao, H., Xie, S., Wang, X., Dai, M., Huang, L., Yuan, Z. 2014. Antibiotic alternatives: the substitution of antibiotics in animal husbandry? Frontiers in Microbiology, vol. 5, p. 69-83. https://doi.org/10.3389/fmicb.2014.00217 PMid:24860564
Desbois, A. P., Lawlor, K. C. 2013. Antibacterial activity of long-chain polyunsaturated fatty acids against Propionibacterium acnes and Staphylococcus aureus. Marine drugs, vol. 11, no. 11, p. 4544-4557. https://doi.org/10.3390/md11114544 PMid:24232668
Directive 2002/32/EC of the European Parliament and of the Council of 7 May 2002 on undesirable substances in animal feed. OJ L 140, 30.5.2002, p. 10-22.
Fiedor, J., Burda, K. 2014. Potential role of carotenoids as antioxidants in human health and disease. Nutrients, vol. 6, no. 2, p. 466-488. https://doi.org/10.3390/nu6020466 PMid:24473231
Gaudin, V., Hedou, C., Rault, A., Verdon, E., 2010. Validation of a Five Plate Test, the STAR protocol, for the screening of antibiotic residues in muscle from different animal species according to European Decision 2002/657/EC. Food Additives and Contaminants A, vol. 27, no. 7, p. 935-952. https://doi.org/10.1080/19440041003677483 PMid:20432095
Gaudin, V., Juhel-Gaugain, M., Morétain, J. P., Sanders, P. 2008. AFNOR validation of a Premi®Test, a microbiological-based screening tube-test for the detection of antimicrobial residues in animal muscle tissue. Food Additives and Contaminants A, vol. 25, no. 12, p. 1451-1464. https://doi.org/10.1080/02652030802429088 PMid:19680855
Gaudin, V., Maris, P., Fuselier, R., Ribouchon, J. L., Cadieu, N., Rault, A. 2004. Validation of a microbiological method: the STAR protocol, a five-plate test, for the screening of antibiotic residues in milk. Food Additives and Contaminants, vol. 21, no. 5, p. 422-433. https://doi.org/10.1080/02652030410001667575 PMid:15204543
Gondová, Z., Kožárová, I., Poláková, Z., Maďarová, M. 2014: Comparison of four microbiological inhibition tests for the screening of antimicrobial residues in the tissues of food-producing animals. Italian Journal of Animal Science, vol. 13, no. 4, p. 728-734. https://doi.org/10.4081/ijas.2014.3521
Haščík, P., Trenbecká, L., Bobko, M., Kačániová, M., Čuboň, J., Kunová, S., Bučko, O. 2016. Effect of diet supplemented with propolis extract and probiotic additives on performance, carcass characteristics and meat composition of broiler chickens. Potravinarstvo, vol. 10, no. 1, p. 223-231. https://doi.org/10.5219/581
Huang, C. B., George, B., Ebersole, J. L. 2010. Antimicrobial activity of n-6, n-7 and n-9 fatty acids and their esters for oral microorganisms. Archives of Oral Biology, vol. 55, no. 8, p. 555-560. https://doi.org/10.1016/j.archoralbio.2010.05.009 PMid:20541177
Ivey, M., Massel, M., Phister, T. G. 2013. Microbial interactions in food fermentations. Annual Reviews in Food Sciences and Technology, vol. 4, p. 141-162. https://doi.org/10.1146/annurev-food-022811-101219 PMid:23190140
Klempová, T., Basil, E., Kubatová, A., Čertik, M. 2013. Biosynthesis of gamma-linolenic acid and betacarotene by Zygomycetes fungi. Biotechnoly Journal, vol. 8, p. 794-800. https://doi.org/10.1002/biot.201200099 PMid:23625863
Kožárová, I., Janošová, J., Máté, D., Tkáčiková, S. 2009. Evaluation of different microbial inhibition tests for the Food Additives and Contaminants 617 detection of sulphamethazine residues in the edible tissues of rabbits. Food Additives and Contaminants A, vol. 26, no. 7, p. 978-987. https://doi.org/10.1080/02652030902839715 PMid:19680973
Kožárová, I., Mačanga, J., Goldová, M., Major, P., Tkáčiková, S. 2011. Detection of maduramycin residues of chickens and pheasants by the screening test for antibiotic residues (STAR). Food Additives and Contaminants A, vol. 28, no. 5, p. 608-618. https://doi.org/10.1080/19440049.2011.561879 PMid:21442501
Magalhães, C. G., De Paiva, C. R., Botelho, B. G., De Oliviera, A. M. G., De Souza, L. F., Nonaka, C. V., Santos, K. V., Farias, L. M., Carvalho, M. A. R. 2012. In-house validation of Premi®Test, a microbiological screening test with solvent extraction, for the detection of antimicrobial residues in poultry muscles. Food Additives and Contaminants A, vol. 29, no. 4, p. 535-540. https://doi.org/10.1080/19440049.2011.627571 PMid:22059477
Niba, A. T., Beal, J. D., Kudi, A. C., Brooks, P. H. 2009. Potential of bacterial fermentation as a biosafe method of improving feeds for pigs and poultry. African Journal of Biotechnology, vol. 8, no. 9, p. 1758-1767.
Pikkemaat, M. G., Rapallini, M. L. B. A., Oostra-Van Dijk, S., Elferink, J. W. A. 2009. Comparison of three microbial screening methods for antibiotics using routine monitoring samples. Analytica Chimica Acta, vol. 637, no. 1-2, p. 298-304. https://doi.org/10.1016/j.aca.2008.08.023 PMid:19286043
R 25. 2006. Screening test for determination of antibiotic residues using bacterial strain (STAR). Available at: http://www.svssr.sk/dokumenty/zakladne_info/Diagnostika/R%2025%20STAR%20test.pdf
R 26. 2006. Detection of inhibitory substances in meat to Premi®Test. Available at: http://www.svssr.sk/dokumenty/zakladne_info/Diagnostika/R%2026%20PREMi%20test.pdf
Regulation (EC) No 178/2002 of the European Parliament and of the Council of 28 January 2002 laying down the general principles and requirements of food law, establishing the European Food Safety Authority and laying down procedures in matters of food safety. OJ L 031, 1.2.2002, p. 1-24.
Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 September 2003 on additives for use in animal nutrition. OJ L 268, 18.10.2003, p. 29-43.
Sayegh, F., Elazzazy, A., Bellou, S., Moustogianni, A., Elkady, A. I., Baeshen, M. N., Aggelis, G. 2016. Production of polyunsaturated single cell oils possessing antimicrobial and anticancer properties. Annals of Microbiology, vol. 66, no. 3, p. 937-948. https://doi.org/10.1007/s13213-015-1176-0
Stead, S., Richmond, S., Sharman, M., Stark, J., Geijp, E., 2005. A new approach for detection of antimicrobial drugs in food: Premi®Test coupled to scanner technology. Analytica Chimica Acta, vol. 529, no. 1-2, p. 83-88. https://doi.org/10.1016/j.aca.2004.09.071
Stead, S., Sharman, J. A., Tarbin, J. A., Gibson, E., Richmond, S., Stark, J., Geijp, E. 2004. Meeting maximum residue limits: an improved screening technique for rapid detection of antimicrobial residues in animal food products. Food Additives and Contaminants, vol. 21, no. 3, p. 216-221. https://doi.org/10.1080/02652030310001647280 PMid:15195469
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
