Microbiological and chemical quality of fresh and frozen whole trout and trout fillets
DOI:
https://doi.org/10.5219/599Keywords:
total viable counts, psychrotrophic bacteria, Pseudomonas, total volatile base-nitrogen, rainbow troutAbstract
The rainbow trout (Oncorhynchus mykiss) is considered as an important fish in the freshwater aquaculture and play a significant role in the human diet. The final quality of fish depends on the chemical and microbiological quality of fish at the time of freezing as well as on other factors including storage temperature and processing. The purpose of the study was to determine the microbiological status of 30 samples cooled and frozen trouts collected from approved farm in the Turiec region, territory of middle Slovakia. Total viable counts (TVCs), psychrotrophic bacteria, Pseudomonas spp. and also total volatile base-nitrogen (TVB-N) and pH were measured in samples of fresh (1. and 7. day of storage at 0 - 2 °C) and frozen whole trout and trout fillets. Frozen samples were stored at -18 °C during 1, 3, and 6 months. Samples were collected from the skin, muscles (sterile) and muscles after filleting. The microbiological quality of samples varied between fresh and frozen (6th month of storage) regarding TVCs and also between samples taken from the skin and muscles after filleting compared to muscle samples collected sterile regarding all tested bacteria. A large number of bacteria (pathogens and spoilage bacteria) enter with the raw material and in particular the skin contamination had a negative impact on the increase of microbial load in fillets. All processing techniques and procedures including filleting therefore must be designed and aimed to minimise contamination and growth of microorganisms in fish. However, based on the results of TVB-N analysis, differences between fresh and frozen samples were found, but all the samples were suitable for human consumption.
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Abbas, K. A., Mohamed, A., Jamilah, B. Ebrahimian, M. 2008. A review on correlations between fish freshness and pH during cold storage. American Journal of Biochemistry and Biotechnology, vol. 4, no. 4, p. 416-421. https://doi.org/10.3844/ajbbsp.2008.416.421 DOI: https://doi.org/10.3844/ajbbsp.2008.416.421
Al-Jasser, M. S., Al-Jasass, F. M. 2014. Study the Chemical, Physical Changes and Microbial Growth as Quality Measurement of Fish. Annual Research and Review in Biology, vol. 4 no. 9, p. 1406-1420. https://doi.org/10.9734/ARRB/2014/7131 DOI: https://doi.org/10.9734/ARRB/2014/7131
Alparslan, Y., Hasanhocaoglu, H., Metin, C., Baygar, T. 2014. Determination of meat quality of sea bass (Dicentrarchus labrax) sold at different selling areas. Emirates Journal of Food and Agriculture, vol. 26, no. 3, p. 293-301. DOI: https://doi.org/10.9755/ejfa.v26i3.16528
Begum, M., Abu Ahmed, A., Das, M., Parveen, S. 2010. A comparative microbiological assessment of five types of selected fishes collected from two different markets. Advances in Biological Research, vol. 4, no. 5, p. 259-265.
Broekaert, K., Heyndrickx, M., Herman, L., Devlieghere, F., Vlaemynck, G. 2011. Seafood quality analysis: Molecular identification of dominant microbiota after ice storage on several general growth media. Food Microbiology, vol. 28, no. 6, p. 1162-1169. https://doi.org/10.1016/j.fm.2011.03.009 DOI: https://doi.org/10.1016/j.fm.2011.03.009
Commission Regulation (EC) No 2074/2005 laying down implementing measures for certain products under Regulation (EC) No 853/2004 and for the organisation of official controls under Regulation (EC) No 854/2004 and Regulation (EC) No 882/2004. OJ L 338, 22.12.2005, 15-18.
Connell, J. J. 1995. Control of fish quality. 4th ed. LONDON, UK : Wiley-Blackwell, Fishing News Books Limited. 256 p. ISBN-13: 978-0-85238-226-4.
Conway, E. J., Byrne, A. 1933. An absorption apparatus for the micro-determination of certain volatile substances. 1. The micro-determination of ammonia. Biochemical Journal, vol. 27, 419-29. PMid:16745115
Diler, O., Altun, S., Calikusu, F., Diler, A. 2000. A study on qualitative and quantitative bacterial flora of the rainbow trout (Oncorhynchus mykiss) living in different fish farms. Turkish Journal of Veterinary and Animal Sciences, vol. 24, p. 251-259.
Fernandes, R. 2009. Microbiology handbook fish and seafood. 1st ed. Cambridge, UK: Rsc Publishing. ISBN: 978-1-905224-76-0. https://doi.org/10.1039/9781847559432 DOI: https://doi.org/10.1039/9781847559432
Franzetti, L., Scarpellini, M. 2007. Characterization of Pseudomonas spp. isolated from foods. Annals of Microbiology, vol. 57, no. 1, p. 39-47. https://doi.org/10.1007/BF03175048 DOI: https://doi.org/10.1007/BF03175048
Gonzalez, C. J., Lopez-Diaz, T. M., Garciia-Lopez, M. L., Prieto, M., Otero, A. 1999. Bacterial microflora of wild brown trout (Salmo trutta), wild pike (Esox lucius), and aquacultured rainbow trout (Oncorhynchus mykiss). Journal of Food Protection, vol. 62, no. 11, p. 1270-1277. PMid:10571316 DOI: https://doi.org/10.4315/0362-028X-62.11.1270
Gram, L. 1993. Inhibitory effect against pathogenic and spoilage bacteria of Pseudomonas strains isolated from spoiled and fresh fish. Journal of Applied Environmental Microbiology, vol. 59, p. 2197-2203. PMid:8357253 DOI: https://doi.org/10.1128/aem.59.7.2197-2203.1993
Hassan, M. A., Shaltout, F. A., Maarouf, A. A., El-Shafey, W. S. 2014. Psychrotrophic bacteria in frozen fish with special reference to pseudomonas species. Benha Veterinary Medical Journal, vol. 27, no. 1, p. 78-83.
Huss, H. H. 1995. Quality and quality changes in fresh fish. FAO Fisheries Technical Paper No. 348. Food and Agriculture Organisation of the United Nations, Rome. ISBN 92-5-103507-5.
ICMSF (International Commission on Microbiological Specifications for Foods). 1986. Sampling plans for fish and shellfish. In ICMSF (Eds.). Microorganisms in Foods 2. Sampling for Microbiological Analysis: Principles and Scientific Applications. 2nd edition, TORONTO, CANADA : University of Toronto Press. p. 181-196.
ISO 4833: 2003. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of microorganisms - Colony-count technique at 30 °C.
ISO 6730: 2005. Milk - Enumeration of colony-forming units of psychrotrophic microorganisms - Colony-count technique at 6.5 °C.
ISO 13720: 2010. Meat and meat products - Enumeration of Pseudomonas spp.
Jay, J. M. 2000. Food preservation with modified atmospheres. In: Jay, J. M. Modern Food Microbiology. GAITHERSBURG, MARYLAND : Aspen publishers, Inc., p. 283-300. ISBN 978-0-8342-1671-6, DOI: https://doi.org/10.1007/978-1-4615-4427-2_14
Jeon, Y. J., Kamil, J. Y., Shahidi, F. 2002. Chitosan as an edible invisible film for quality preservation of herring and Atlantic cod. Journal of Agricultural and Food Chemistry, vol. 50, no. 18, p. 5167-5178. https://doi.org/10.1021/jf011693l DOI: https://doi.org/10.1021/jf011693l
Khidhir, Z.K., Jaff, B. M. A., Saleh, H. H. 2014. Assessment of the microbial quality of five types of Iraqi fresh fish in Sulaimania markets. Journal of Zankoy Sulaimani- Part A, vol. 16, p. 251- 260. DOI: https://doi.org/10.17656/jzs.10328
Kunová, S., Bobková, A., Lopašovský, L., Kačániová, M. 2014. Microbiological evaluation of poultry sausages stored at different temperatures. Potravinarstvo, vol. 8, no. 1, p. 141-145. https://doi.org/10.5219/338 DOI: https://doi.org/10.5219/338
Ninan, G., Zynudheen, A.A, Joseph, J. 2011. Effect of Chilling on Microbiological, Biochemical and Sensory Attributes of Whole Aquacultured Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792). Journal of Aquaculture Research and Development, S5 https://doi.org/10.4172/2155-9546.S5-001 DOI: https://doi.org/10.4172/2155-9546.S5-001
Özogul, F., Özogul, Y. 2000. Comparison of methods used for determination of total volatile basic nitrogen (TVB-N) in rainbow trout (Oncorhynchus mykiss). Turkish Journal of Zoology, vol. 24, p. 113-120.
Özogul, F., Yavuzer, E., Özogul, Y., Kuley, E. 2013. Comparative quality loss in wild and cultured rainbow trout (Oncorhynchus mykiss) during chilling storage. Food Sciences and Technology Research, vol. 19, no. 3, p. 450-454. https://doi.org/10.3136/fstr.19.445 DOI: https://doi.org/10.3136/fstr.19.445
Popelka, P., Nagy, J., Pipová, M., Marcinčák, S., Lenhardt, L. 2014. Comparison of chemical, microbiological and histological changes in fresh, frozen and double frozen rainbow trout (Oncorhynchus mykiss). Acta Veterinaria Brno, vol. 83, p. 157-161. https://doi.org/10.2754/avb201483020157 DOI: https://doi.org/10.2754/avb201483020157
Rezaei, M., Hosseini, S. F. 2008. Quality assessment of farmed rainbow trout (Oncorhynchus mykiss) during chilled storage. Journal of Food Sciences, vol. 73, no. 6, p. 93-96. https://doi.org/10.1111/j.1750-3841.2008.00792.x DOI: https://doi.org/10.1111/j.1750-3841.2008.00792.x
Shamsuzzaman, M. M., Mazumder, S. K., Siddique, M. A., Miah, M. N. U. 2011. Microbial quality of hilsa shad (Tenualosa ilisha) at different stages of Processing. Journal of the Bangladesh Agricultural University, vol. 9, no. 2, p. 339-344. DOI: https://doi.org/10.3329/jbau.v9i2.11049
Shewan, J. M. 1961. The microbiology of seawater fish. In: Borgstrom, G. Fish as Food. LONDON, UK : Academic Press, p. 487-560. https://doi.org/10.1016/B978-0-12-395569-2.50020-0 DOI: https://doi.org/10.1016/B978-0-12-395569-2.50020-0
Sivertsvik, M., Jeksrud, W. K., Rosnes, J. T. 2002. A review of modified atmosphere packaging of fish and fishery products - significance of microbial growth, activities and safety. International Journal of Food Sciences and Techonology, vol. 37, p. 107-27. https://doi.org/10.1046/j.1365-2621.2002.00548.x DOI: https://doi.org/10.1046/j.1365-2621.2002.00548.x
Stansby, M. E., Olcott, H. S. 1963. Industrial Fishery Technology Composition of Fish. Reinhold Publishing Corporation. p. 339-349.
van den Broek, M. J. M., Mossel D. A. A., Mol, H. 1984. Microbiological quality of retail fresh fish fillets in The Netherlands. International Journal of Food Microbiology, vol. 1, p. 53-61. https://doi.org/10.1016/0168-1605(84)90008-4 DOI: https://doi.org/10.1016/0168-1605(84)90008-4
Velu, S., Bakar, A. F., Mahyudin, N. A., Saari, N., Zaman, M. Z. 2013. Effect of modified atmosphere packaging on microbial flora changes in fishery products. International Food Research Journal, vol. 20, no. 1, p. 17-26.
Vogel, B. F., Venkateswaran, K., Satomi, M., Gram, L. 2005. Identification of Shewanella baltica as the most important H2S-producing species during iced storage of Danish marine fish. Applied and Environmental Microbiology, vol. 71, p. 6689-97. https://doi.org/10.1128/AEM.71.11.6689-6697.2005 DOI: https://doi.org/10.1128/AEM.71.11.6689-6697.2005
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