Comparative analysis of antioxidant activity and phenolic compounds in the fruits of Aronia spp.


  • Yulia Vinogradova N. V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Department of the Native Flora, Botanicheskaya 4, 127276, Moscow, Russia, Tel.: +74999779136
  • Olena Vergun M. M. Gryshko National Botanical Garden of the NAS of Ukraine, Cultural Flora Department, Timiryazevska 1, 04014, Kyiv, Ukraine, Tel.: +380975398541
  • Olga Grygorieva M. M. Gryshko National Botanical Garden of the NAS of Ukraine, Department of Fruit Plants Acclimatisation, Timiryazevska 1, 04014, Kyiv, Ukraine, Tel.: +380671988082
  • Eva Ivanišová Slovak University of Agricultural in Nitra, Faculty of Biotechnology and Food Resources, Department of Technology and Quality of Plant Products, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia, Tel: +421376414421
  • Ján Brindza Slovak University of Agricultural in Nitra, Faculty of Agrobiology and Food Resources, Institute of Biological Conservation and Biosafety, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia, Tel: +421376414787



Aronia spp, antioxidant activity, polyphenols, flavonoids, phenolic acids


Chokeberry (Aronia Medik.) is a non-traditional fruit plant known as a rich source of biologically active compounds and inhibits the numerous biological activities. We compared the antioxidant activity and phenolic compounds of fruits between widely cultivated Aronia mitschurinii (AM-TCH, from Tchekhov district; AM-D, from Dmitrov district; AM-OZ, from Orekhovo-Zuevsky district of Moscow region, Russia) and introduced North American Aronia species (Aronia arbutifolia (AA-M), A. melanocarpa (AML-M), A. × prunifolia (AP-M), which have not been planted yet in the arboretum of Main Botanical Garden of the Russian Academy of Sciences (Moscow). Studying samples were collected in their secondary distribution range. Ethanolic extracts were determined for antioxidant capacity (antioxidant activity by DPPH and phosphomolybdenum methods, the total content of polyphenols, flavonoids, phenolic acids) and measured spectrophotometrically. As standards were used Trolox (TE) for antioxidant activities, gallic acid (GAE) for polyphenol content, quercetin (QE) for flavonoid content, caffeic acid (CAE) for phenolic acid content. The antioxidant activity by DPPH method in ethanol extracts of investigated plants was from 6.96 (AM-D) to 8.89 (AM-OZ) mg TE.g-1 DW. Reducing the power of investigated extracts exhibited activity from 151.47 (AM-OZ) to 297.8 (AA-M) mg TE.g-1 DW. The content of polyphenol compounds determined from 25.98 (AM-TCH) to 54.39 (AA-M) mg GAE.g-1 DW, phenolic acids content was from 7.76 (AP-M) to 11.87 (AM-D) mg CAE.g-1 DW and the content of flavonoids detected from 8.12 (AM-OZ) to 16.62 (AM-D) mg QE.-1 DW. Obtained data showed a strong correlation between the content of polyphenol compounds and reducing the power of extracts (r = 0.700), between flavonoids and phenolic acids (r = 0.771) and also between phenolic acids and reducing power (r = 0.753) in Aronia ethanol extracts. Fruits of investigated species of Aronia can be propagated as a source of polyphenol compounds with antioxidant activity and obtained results may use for farther pharmacological study.


Download data is not yet available.


Metrics Loading ...


Alam, M. N., Bristi, N. J., Rafiquzzaman, M. 2013. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, vol. 21, no. 2, p. 143-152. DOI:

Aly, A., Maraei, R., El-Leel, A. O. 2019. Comparative study of some bioactive compounds and their antioxidant activity of some berry types. Potravinarstvo Slovak Journal of Food Sciences, vol. 13, no. 1, p. 515-523. DOI:

Banjari, I., Misir, A., Šavikin, K., Jokić, S., Molnar, M., De Zoysa, H. K. S., Waisundara, V. Y. 2017. Antidiabetic effect of Aronia melanocarpa and its other therapeutic properties. Frontiers in Nutrition, vol. 4, 6 p. DOI:

Bolser, A. J., Alan, R. R., Smith, D. A., Li, L., Seeram, P. N., McWilliams, R. S. 2013. Birds select fruits with more anthocyanins and phenolic compounds during autumn migration. The Wilson Journal of Ornithology, vol. 125, no. 1, p. 97-108. DOI:

Brand, H. M., Connolly, A. B., Levine, H. L., Richards, J. T., Shine, M. S., Spencer, E. L. 2017. Anthocyanins, total phenolics, ORAC and moisture content of wild and cultivated dark-fruited Aronia species. Scientia Horticulturae, vol. 224, p. 332-342. DOI:

Bräunlich, M. 2013. Bioactive constituents in Aronia berries : dissertation theses. University of Oslo. 110 р. Available at:

Brindza, J., Grygorieva, O., Klymenko, S., Vergun, O., Mareček, J., Ivanišová, E. 2019. Variation of fruits morphometric parameters and bioactive compounds of Asimina triloba (L.) Dunal germplasm collection. Potravinarstvo Slovak Journal of Food Sciences, vol. 13, no. 1, p. 1-7. DOI:

Chrubasik, C., Li, G., Chrubasik, S. 2010. The clinical effectiveness of chokeberry: a systematic review. Phytotherapy Research, vol. 24, no. 8, p. 1107-1114. DOI:

Daskalova, E., Delchev, S., Peeva, Y., Vladimirova-Kitova, L., Kratchanova, M., Kratchanov, C., Denev, P. 2015. Antiatherogenic and Cardioprotective Effects of Black Chokeberry (Aronia melanocarpa) Juice in Aging Rats. Evidence-Based Complementary and Alternative Medicine, vol. 2015, 10 p. DOI:

Denev, P. N., Kratchanov, C. G., Ciz, M., Lojek, A., Kratchanova, M. G. 2012. Bioavailability and antioxidant activity of black chokeberry (Aronia melanocarpa) polyphenols: in vitro and in vivo evidences and possible mechanisms of action: A review. Comprehensive Reviews in Food Science and Food Safety, vol. 11, no. 5, p. 471-489. DOI:

Denev, P., Číž, M., Kratchanova, M., Blazheva, D. 2019. Black chokeberry (Aronia melanocarpa) polyphenols reveal different antioxidant, antimicrobial and neutrophil-modulating activities. Food Chemistry, vol. 284, p. 108-117. DOI:

Denev, P., Kratchanova, M., Petrova, I., Klisurova, D., Georgiev, Y., Ognyanov, M., Yanakieva, I. 2018. Black chokeberry (Aronia melanocarpa (Michx.) Elliot) fruits and functional drinks differ significantly in their chemical composition and antioxidant activity. Journal of Chemistry, vol. 2018, 12 p. DOI:

Farmakopea Polska. 1999. Title. The Polish Pharmaceutical Society. Available at:

Gao, N., Wang, Y., Jiao, X., Chou, S., Li, E., Li, B. 2018. Preparative purification of polyphenols from Aronia melanocarpa (Chokeberry) with cellular antioxidant and antiproliferative activity. Molecules, vol. 23, no. 1, 14 p. DOI:

Gasiorowski, K., Szyba, K., Brokos, B., Kołaczyńska, B., Jankowiak-Włodarczyk, M., Oszmiański, J. 1997. Antimutagenic activity of anthocyanins isolated from Aronia melanocarpa fruits. Cancer Letters, vol. 119, no. 1, p. 37-46. DOI:

Gralec, M., Wawer, I., Zawada, K. 2019. Aronia melanocarpa: phenolics composition and antioxidant properties changes during fruit development and ripening. Emirates Journal of Food and Agriculture, vol. 31, no. 3, p. 214-221. DOI:

Hardin, J. W. 1973. The enigmatic chokeberries (Aronia, Rosaceae). Bulletin of the Torrey Botanical Club, vol. 100, no. 3, p. 178-184. DOI:

Ho, T. T. G., Bräunlich, M., Austarheim, I., Wandensteen, H., Malterud, K. E., Slimestad, R., Barsett, H. 2014. Immunomodulating activity of Aronia melanocarpa polyphenols. Molecules, vol. 15, no. 7, p. 11626-11636. DOI:

Horčinová Sedlačková, V., Grygorieva, O., Fatrcová-Šramková, K., Vergun, O., Vinogradova, Y., Ivanišová, E., Brindza, J. 2018. The morphological and antioxidant characteristics of inflorescences within wild-growing genotypes of elderberry (Sambucus nigra L.). Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, p. 444-453. DOI:

Jakobek, L., Drenjančević, M., Jukić, V., Šeruga, M., Turalija, A., Milić, M. 2012. Polyphenols, anthocyanins and antiradical activity of chokeberries. Electronic Journal of Environmental, Agricultural and Food Chemistry, vol. 11, no. 2, p. 76-84.

Jakobek, L., Seruga, M. 2012. Influence of anthocyanins, flavonols and phenolic acids on the antiradical activity of berries and small fruits. International Journal Journal of Food Properties, vol. 15, no. 1, p. 122-133. DOI:

Jakobek, L., Šeruga, M., Medvidović-Kosanović, M., Novak, I. 2007. Antioxidant activity and polyphenols of Aronia in comparison to other berry species. Agriculturae Conspectus Scientificus, vol. 72, no. 4, p. 301-306.

Jeong, J.-M. 2008. Antioxidative and antiallergic effects of Aronia (Aronia melanocarpa) extract. Journal of Korean Society of Food Science and Nutrition, vol. 37, no. 9, p. 1109-1113. DOI:

Jurikova, T., Mlcek, J., Skrovankova, S., Sumczynski, D., Sochor, J., Hlavacova, I., Snopek, L., Orsavova, J. 2017. Fruits of black chokeberry Aronia melanocarpa in the prevention of chronic diseases. Molecules, vol. 22, no. 6, 23 p. DOI:

Kapci, B., Neradová, E., Čížkova, H., Voldřich, M., Rajchl, A., Capanoglu, E. 2013. Investigating the antioxidant potential of chokeberry (Aronia melanocarpa) products. Journal of Food and Nutrition Research, vol. 52, no. 4, p. 219-229.

Kokotkiewicz, A., Jaremicz, Z., Luczkiewicz, M. 2010. Aronia Plants: A Review of traditional use, biological activities, and perspectives for modern medicine. Journal of Medicinal Food, vol. 13, no. 2, p. 255-269. DOI:

Koponen, J. M., Happonen, A. M., Mattila, P. H., Torronen, A. R. 2007. Contents of anthocyanins and ellagitannins in selected foods consumed in Finland. Journal of Agricultural and Food Chemistry, vol. 55, no. 4, p. 1612-1619. DOI:

Kulling, S. E., Rawel, H. M. 2008. Chokeberry (Aronia melanocarpa). A review on the characteristic components and potential health effects. Planta Medica, vol. 74, no. 13, p. 1625-1634. DOI:

Kumar, N., Goel, N. 2019. Phenolic acids: natural versatile molecules with promising therapeutic applications. Biotechnology Reports, vol. 24, e00370. DOI:

Lachowicz, S., Oszmianski, J., Seliga, L., Pluta, S. 2017. Phytochemical composition and antioxidant capacity of seven saskatoon berry (Amelanchier alnifolia Nutt.) genotpes grown in Poland. Molecules, vol. 22, no. 5, 17 p. DOI:

Martin, D. A., Taheri, R., Brand, M. H., Draghi, A., Sylvester, F. A., Bolling, B. W. 2014. Anti-inflammatory activity of Aronia berry extracts in murine splenocytes. Journal of Functional Foods, vol. 8, p. 68-75. DOI:

Matsumoto, M., Hara, H., Chiji, H., Kasai, T. 2004. Gastroprotective effect of red pigments in black chokeberry fruit (Aronia melanocarpa Elliot) on acute gastric hemorrhagic lesions in rats. Journal of Agricultural and Food Chemistry, vol. 52, no. 8, p. 2226-2229. DOI:

Mattila, P., Hellström, J., Törrönen, R. 2006. Phenolic acids in berries, fruits, and beverages. Journal of Agricultural and Food Chemistry, vol. 54, no. 19, p. 7193-7199. DOI:

Mayer-Miebach, E., Adamiuk, M., Behsnilian, D. 2012. Stability of Chokeberry Bioactive Polyphenols during Juice Processing and Stabilization of a Polyphenol-Rich Material from the By-Product. Agriculture, vol. 2, no. 3, p. 244-258. DOI:

Moharram, H. A., Youssef, M. M. 2014. Methods for determining the antioxidant activity: a review. Alexandria Journal of Food Science and Technology, vol. 11, no. 1, p. 31-42. DOI:

Montrose, D. C., Horelik, N. A., Madigan, J. P., Stoner, G. D., Wang, L.-S., Bruno, R. S., Park, H. J., Giardina, C., Rosenberg, D. W. 2011. Anti-inflammatory effects of freeze-dried black raspberry powder in ulcerative colitis. Carcinogenesis, vol. 32, no. 3, p. 343-350. DOI:

Nguyen, L., Hwang, E.-S. 2016. Quality characteristics and antioxidant activity of yogurt supplemented with Aronia (Aronia melanocarpa) juice. Preventive Nutrition and Food Science, vol. 21, no. 4, p. 330-337. DOI:

Olas, B., Kedzienska, M., Wachowicz, B., Stochmal, A., Oleszek, W. 2010. Effects of polyphenol-rich extract from berries of A. melanocarpa on the makers of oxidative stress and blood platelet activation. Platelets, vol. 21, no. 4, p. 274-281. DOI:

Olas, B., Wachowicz, B., Nowak, P., Kedzienska, M., Tomczak, A., Stochmal, A., Oleszek, W., Jeziorski, A., Piekarski, J. 2008. Studies on antioxidant properties of polyphenol-rich extract from berries of Aronia melanocarpa in blood platelets. Journal of Physiology and Pharmacology, vol. 59, no. 4, p. 823-835.

Oszmianski, J., Lachowicz, S. 2016. Effect of production of dried fruits and juice from chokeberries (Aronia melanocarpa L.) on the content and antioxidative activity of bioactive compounds. Molecules, vol. 21, no. 8, 14 p. DOI:

Oszmianski, J., Wojdylo, A. 2005. Aronia melanocarpa phenolics and their antioxidant activity. European Food Research and Technology, vol. 221, p. 809-813. DOI:

Pisoschi, A. M., Pop, A., Cimpeanu, C., Predoi, G. 2016. Antioxidant capacity determination in plants and plant-derived products: a review. Oxidative Medicine and Cellular Longevity, vol. 2016, 37 p. DOI:

Prieto, P., Pineda, M., Aguilar, M. 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry, vol. 269, no. 2, p. 337-241. DOI:

Rodríguez-Werner, M., Winterhalter, P., Esatbeyoglu, T. 2019. Phenolic composition, radical scavenging activity and an approach for authentication of Aronia melanocarpa berries, juice, and pomace. Journal of Food Science, vol. 84, no. 7, p. 1791-1798. DOI:

Rugină, D., Sconţa, Z., Leopold, L., Pintea, A., Bunea, A., Socaciu, C. 2012. Antioxidant activities of Chokeberry extracts and the cytotoxic action of their anthocyanin fraction on HeLa human cervical tumor cells. Journal of Medicinal Food, vol. 15, no. 8, p. 700-706. DOI:

Sánchez-Moreno, C., Larrauri, A., Saura-Calixto, F. 1998. A procedure to measure the antioxidant efficiency of polyphenols. Journal of the Science of Food and Agriculture, vol. 76, no. 2, p. 270-276.<270::AID-JSFA945>3.0.CO;2-9 DOI:<270::AID-JSFA945>3.0.CO;2-9

Shafii, Z. A., Basri, M., Malek, E. A., Ismail, M. 2017. Phytochemical and antioxidant properties of Manilkara zapota (L.) P roen fruit extracts and its formulations for cosmeceutical application. Asian Journal of Plant Science and Research, vol. 7, no. 3, p. 29-41. Available at:

Sharif, T., Alhosin, M., Auger, C., Minker, C., Kim, J.-H., Etienne-Selloum, N., Bories, P., Gronemeyer, H., Lobstein, A., Bronner, C., Fuhrmann, G., Schini-Kerth, V. B. 2012. Aronia melanocarpa juice induces a redox-sensitive p73-related caspase 3-dependent apoptosis in human leukemia cells. PLoS One, vol. 7, no. 3, e32526. DOI:

Sidor, A., Gramza-Michałowska, A. 2019. Black chokeberry Aronia melanocarpa L. – a qualitative composition, phenolic profile and antioxidant potential. Molecules, vol. 24, no. 20, 57 p. DOI:

Singleton, V. L., Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagent. American Journal of Enology and Viticulture, vol. 16, p. 144-158.

Skvortsov, A. K., Maitulina, Yu. K. 1982. On distinctions of cultivated black-fruited aronia from its wild ancestors. Bulletin of the Central Botanical Garden, AN SSSR, vol. 126, p. 35-40. Available at:

Skvortsov, A. K., Maitulina, Yu. K., Gorbunov, Yu. N. 1983. Cultivated black-fruited aronia: place, time, and probable mechanism of formation. Bull. MOIP, Otd. Biol., vol. 88, no. 3, p. 88-96. Available at:

Slimestad, R., Torskangerpoll, K., Nateland, H. S., Johannessen, T., Giske, N. H. 2005. Flavonoids from black chokeberries, Aronia melanocarpa. Journal of Food Composition and Analysis, vol. 18, no. 1, p. 61-68. DOI:

Šnebergrová, J., Čižková, H., Neradová, E., Kapci, B., Rajchl, A., Voldřich, M. 2014. Variability of characteristic components of Aronia. Czech Journal of Food Sciences, vol. 32, no. 1, p. 25-30. DOI:

Strugała, P., Gabrielska, J. 2014. Biological activity and stability in vitro of polyphenolic extracts as potential dietary supplements. Advances in Hygiene and Experimental Medicine, vol. 68, p. 1028-1035. DOI:

Suvajdžić, L., Krstić, T., Stojanović, S., Grujić-Letić, N., Ilić, N., Milankov, O., Gigov, S., Petrović, M., Svirčev, Z., Ilić, N. 2017. Activity of Serbian Aronia prunifolia against Prototheca wickerhamii and Prototheca zopfii. Acta Scientiae Veterinariae, vol. 45, p. 9. DOI:

Szopa, A., Kokotkiewicz, A., Kubica, P., Banaszczak, P. Wojtanowska-Krośniak, A., Krośniak, M., Marzec-Wróblewska, U., Badura, A., Zagrodzki, P., Bucinski, A., Luczkiewicz, M., Ekiert, H. 2017. Comparative analysis of different groups of phenolic compounds in fruit and leaf extracts of Aronia sp.: A. melanocarpa, A. arbutifolia, and A. ×prunifolia and their antioxidant activities. European Food Research and Technology, vol. 243, p. 1645-1657. DOI:

Taheri, R., Connolly, B. A, Brand, M. H., Bolling, B. W. 2013. Underutilized сhokeberry (Aronia melanocarpa, Aronia arbutifolia, Aronia prunifolia) accessions are rich sources of anthocyanins, flavonoids, hydroxycinnamic acids, and proanthocyanidins. Journal of Agricultural and Food Chemistry, vol. 61, p. 8581-8588. DOI:

Tolić, M.-T., Jurčević Landeka, I., Krbavčić Panjkota, I., Marković, K., Vahčić, N. 2015. Phenolic content, antioxidant activity and quality of chokeberry (Aronia melanocarpa) products. Food Technology and Biotechnology, vol. 53, no. 2, p. 171-179. DOI:

Valcheva-Kuzmanova, S. V., Belcheva, A. 2006. Current knowledge of Aronia melanocarpa as a medicinal plant. Folia Medica (Plovdiv), vol. 48, no. 2, p. 11-17.

Veljković, J., Brcanović, J., Pavlović, A., Mitić, S., Kaličanin, B., Mitić, M. 2014. Bagged Aronia melanocarpa tea: phenolic profile and antioxidant activity. Scientific Journal of the Faculty of Medicine in Niš, vol. 31, no. 4, p. 245-252. DOI:

Vinogradova, Y. K, Kuklina, A. G. 2012. The resource potential of invasive plant species. Possibilities of using alien species. Moscow, Russia : GЕОS. 186 p.

Vinogradova, Y. K, Kuklina, A. G. 2014. Aronia mitschurinii: from creation to naturalization. Moscow, Russia : GEOS. 137 p.

Vinogradova, Y. K., Grygorieva, O., Vergun, O., Brindza, J. 2017. Morphological characteristics for fruits of Aronia mitchurinii A.K. Skvortsov & Maitul. Potravinarstvo Slovak Journal of Food Sciences, vol. 11, no. 1, p. 754-760. DOI:

Vinogradova, Y. K., Shelepova, O., Vergun, O., Grygorieva, O., Kuklina, A., Brindza, J. 2018. Differences between Aronia Medik. Taxa on the Morphological and Biochemical Characters. Environmental Research, Engineering and Management, vol. 74, no. 4, 10 p. DOI:

Wangensteen, H., Bräunlich, M., Nikolic, V., Malterud K. E., Slimestad R., Barsett H. 2014. Anthocyanins, proanthocyanidins and total phenolics in four cultivars of aronia: Antioxidant and enzyme inhibitory effects. Journal of Functional Foods, vol. 7, p. 746-752. DOI:

Widén, C., Ekholm, A., Piwowar-Zail, D., Rumpunen, K. 2012. Antioxidant activity of polyphenol rich fruits on human erythrocytes. Acta Horticulturae, vol. 926, p. 669-674. DOI:

Yang, H., Kim, Y.-J., Shin, Y. 2019. Influence of ripening stage and cultivar on physicochemical properties and antioxidant compositions of Aronia grown in South Korea. Foods, vol. 8, no. 12, 16 p. DOI:

Žlabur Šic, J., Dobričević, N., Pliestić, S., Galić, A., Bilić, P. D., Voća, S. 2017. Antioxidant potential of fruit juice with added chokeberry powder (Aronia melanocarpa). Molecules, vol. 22, no. 12, 11 p. DOI:



How to Cite

Vinogradova, Y., Vergun, O., Grygorieva, O., Ivanišová, E., & Brindza, J. (2020). Comparative analysis of antioxidant activity and phenolic compounds in the fruits of Aronia spp. Potravinarstvo Slovak Journal of Food Sciences, 14, 393–401.

Most read articles by the same author(s)

1 2 3 4 5 6 > >> 

Similar Articles

You may also start an advanced similarity search for this article.