Phenolic content and antioxidant activity of Echinocystis lobata (Mich.) Torr. ET Gray (Cucurbitaceae)


  • Yulia Vinogradova , N. V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya, 4, 127276 Moscow, Russia, Tel.: +74999779136
  • Olga Shelepova N. V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya, 4, 127276 Moscow, Russia, Tel.: +74999779136
  • Olena Vergun M. M. Gryshko National Botanical Garden of the NAS of Ukraine, Cultural Flora Department, Timiryazevska 1, 04014, Kyiv, Ukraine, Tel.: +380975398541
  • Olga Grygorieva
  • Jan Brindza Slovak University of Agricultural in Nitra, Faculty of Agrobiology and Food Resources, Institute of Biological Conservation and Biosafety, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia, Tel: +421376414787



Echinocystis lobata, leaves, flowers, fruits, seed, biological activity


Echinocystis lobata (Mich.) Torr. et Gray extracts were investigated for their bioactive compounds and their potential for natural antioxidant properties. In vitro antioxidant activity of methanol, ethanol, and water extracts were evaluated by
1,1-diphenyl-picrylhydrazyl (DPPH) radical scavenging activity. Total phenolic content (TPC) and total flavonoid content (TFC) of fruits and seeds were determined. TPC is a fairly constant value and varies weakly both among the different organs of Echinocystis lobata and among the geographical locations of the populations studied. Like other invasive species, Echinocystis lobata is superior to many other plants in terms of the total amount of flavonoids contained in its fruits and seeds. The dried fruits contain more flavonoids than the seeds. Seeds from Slovak populations have higher flavonoid content than those from Russian populations. The results demonstrated that Echinocystis lobata can be a good source of natural antioxidants and have a great potential for commercialization, especially in the pharmaceutical industry.


Download data is not yet available.


Metrics Loading ...


Abifarin, T. O., Afolayan, A. J., Otunola, G. A. 2019. Phytochemical and antioxidant activities of Cucumis africanus L.f.: a wild vegetable of South Africa. Journal of Evidence-Based Integrative Medicine, vol. 24, p. 1-8. DOI:

Atanassova, M., Georgieva, S., Ivancheva, K. 2011. Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs. Journal of the University of Chemical Technology and Metallurgy, vol. 46, no. 1, p. 81-88.

Budantsev, A. L., Lesiovskaya, E. E. 2014. Dikorastushchiye poleznyye rasteniya Rossii [Useful wild plants in Russia]. St. Petersburg: State Chemical Pharmaceutical Academy. 663 p. (In Russian)

Burton, Т., Dunlap, T., Dong, H., Li, G., Bolton, J., Soejarto, D., van Breemen, R. B. 2015. American Indian botanicals: possible alternatives to hormone therapy during menopause. Planta Med., vol. 81 - PM13. DOI:

Coxworth, E. C. M. 1965. Oil and protein content, and oil composition of seeds of some plants of the Canadian praires. J. of the Amer. Oil Chemist’s Society, vol. 42, no.10, p. 891-894. DOI:

Ghafar, F., Nazrin, T. N. N. T., Salleh, M. R. M. S, Hadi, N. N, Ahmad, N., Hamzah, A. A., Yusof, Z. A. M., Azman, I. N. 2017. Total phenolic content and total flavonoid content in Moringa oleifera seed. Science Heritage Journal, Galeri Warisan Sains (GWS), vol. 1, no. 1, p. 23-25. DOI:

Gillard, D. F., Walton D. C. 1976. Abscisic acid metabolism by a cell-free preparation from Echinocystis lobata liquid endosperm. Plant Physiol., vol. 58, p. 790-795. DOI:

Golivets, M. 2014. Variation quantitative seed traits of Echinocystis lobata (Michx.) Torr. et Gray (Cucurbitaceae). Modern Phytomorphology, vol. 4. p. 43-44.

Grygorieva, O., Klymenko, S., Vergun, O., Mňahončakova, E., Brindza, J., Terentjeva, M., Ivanišova, E. 2020. Evaluation of the antioxidant activity and phenolic content of Chinese quince (Pseudocydonia sinensis Schneid.) fruit. Acta Sci. Pol. Technol. Aliment., vol. 19, no. 1, p. 25-36. DOI:

Grygorieva, O., Kucharska, A. Z., Piórecki, N., Klymenko, S., Vergun, O., Brindza, J. 2018. Antioxidant activities and phenolic compounds in fruits of various genotypes of American persimmon (Diospyros virginiana L.). Acta Sci. Pol. Technol. Aliment., vol. 17, no. 2, p. 117-124. DOI:

Guzzetti, L., Galimberti, A., Bruni, I., Magoni, C., Ferri, M., Tassoni, A., Sangiovanni, E., Dell’Agli, V., Labra, M. 2017. Bioprospecting on invasive plant species to prevent seed dispersal. Scientific Reports, vol. 7, p. 13799. DOI:

Horčinová Sedláčková, V., Grygorieva, O., FatrcováŠramková, K., Vergun, O., Vinogradova, Y., Ivanišová, E., Brindza, J. 2018. The morphological and antioxidant characteristics of inflorescences within wild-growing genotypes of elderberry (Sambucus nigra L.). Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, p. 444-453. DOI:

Horčinová Sedláčková, V., Grygorieva, O., Vergun, O. M., Vinogradova, Y., Brindza, J. 2019. Comparison of selected characteristics of cultivars and wild-growing genotypes of Sambucus nigra in Slovakia. Biosystems Diversity, vol. 27, p. 56-61. DOI:

Ielciu, I., Hanganu, D., Păltinean, R., Vlase, L., Frédérich, M., Gheldiu, A., Benedec, D., Crişan, G. 2018. Antioxidant capacity and polyphenolic content of the Echinocystis lobata (Michx.) Torr. et A.Gray flowers. Pak. J. Pharm. Sci., vol. 31, no. 2, p. 677-683.

Ielciu, I., Vlase, L., Frédérich, M., Hanganu, D., Păltinean, R., Cieckiewicz, E., Olah, N., Gheldiu, A., Crişan, G. 2017. Polyphenolic profile and biological activities of the leaves and aerial parts of Echinocystis Lobata (Michx.) Torr. et A. Gray (Cucurbitaceae). Farmacia, vol. 65, no. 2, p. 179-183.

Ivanišová, E., Blašková, M., Terentjeva, M., Grygorieva, O., Vergun, O., Brindza, J., Kačániová, M. 2020. Biological properties of sea buckthorn (Hippophae rhamnoides L.) derived products. Acta Sci. Pol. Technol. Aliment., vol. 19, no. 2, p. 1-11. DOI:

Ivanišová, E., Grygorieva, O., Abrahamová, V., Schubertova, Z., Terentjeva, M., Brindza, J. 2017. Characterization of morphological parameters and biological activity of jujube fruit (Ziziphus jujuba Mill.). Journal of Berry Research, vol. 7, no. 4, p. 249-260. DOI:

Kim, Y. O., Lee, E. J. 2011. Comparison of phenolic compounds and the effects of invasive and native species in East Asia: Support for the novel weapons hypothesis. Ecological Research, vol. 26, no. 1, p. 87-94. DOI:

Klymenko, S., Grygorieva, O., Brindza, J. 2017. Less known species of fruit crops. Nitra: Slovak University of Agriculture in Nitra, 104 p. DOI:

Krauze-Baranowska, M., Cisowski, W. 1996. Flavonoids from Echinocystis lobata and Echinocystis wrightii. Polish Journal of Chemistry, vol. 70, no. 4, p. 430-436.

Maema, L. P., Potgieter, M. J., Samie, A. 2019. Dataset on preliminary phytochemical analysis and antioxidant activity of selected invasive alien plant species used in the treatment of sexually transmitted infections in Waterberg district, South Africa. Data in brief, vol. 25, p. 104281. DOI:

Melymuka, M., Junge, C., Burnham, R. 2005. Echinocystis lobata. Plant Diversity, vol. 64, no. 1, p. 1-4.

Monka, A., Grygorieva, O., Chlebo, P., Brindza, J. 2014. Morphological and antioxidant characteristics of quince (Cydonia oblonga Mill.) and Chinese quince fruit (Pseudocydonia sinensis Schneid.). Potravinarstvo Slovak Journal of Food Sciences, vol. 8, p. 333-340. DOI:

Panosyan, A. G. 1985. Vliyanie kukurbitacinov brionii na biosintez ejkozanoidov v lejkocitah cheloveka [Effect of bryonia cucurbitacin on eicosanoid biosynthesis in human leukocytes]. Bioorganic chemistry, vol. 11, no. 2, p. 264-269. (In Russian)

Richardson, D. M., Pyšek, P. 2006. Plant invasions: Merging the concepts of species invasiveness and community invisibility. Prog. Phys. Geogr., vol. 30, no. 3, p. 409-431. DOI:

Rodge, S., Biradar, S. D. 2017. Antioxidant activity of some medicinal plants of family Cucurbitaceae. Worlds Journal of Pharmaceutical Research, vol. 6, no. 4, p. 946-951. DOI:

Sari, N. M., Kuspadini, H., Amirta, R., Kusuma, I. W. 2018. Antioxidant activity of an invasive plant, Melastoma malabathricum and its potential as herbal tea product. 1st International Conference on Tropical Studies and its Application. Earth and Environmental Science, vol. 144, p. 012029. DOI:

Shafii, Z. A., Basri, M., Malek, E. A., Ismail, M. 2017. Phytochemical and antioxidant properties of Manilkara zapota (L.) P. Royen fruit extracts and its formulations for cosmeceutical application. Asian Journal of Plant Science and Research, vol. 7, no. 3, p. 29-41.

Shelepova, O. V., Vinogradova, J. K. 2017. Chemical composition of oil in the seed of Echinocystis lobata (Mich.) Torr. et Gray. Skvortsovia, vol. 3, no. 2, p. 88-90.

Shelepova, O. V., Vinogradova, Yu. K., Vergun, O., Grygorieva, O. V., Brindza, J. 2020. Assessment of flavonoids and phenolic compound accumulation in invasive Solidago сanadensis L. in Slovakia. Potravinarstvo Slovak Journal of Food Sciences, vol. 14, p. 587-594. DOI:

Shelepova, O., Kuklina, A., Vinogradova, Yu. 2016. Seeds of Echinocystis lobata (Mich.) Torr. et Gray, Cucurbitaceae as a promising pharmacological agent. Agrobiodiversity for improving nutrition, health and life quality. Nitra, p. 416-420.

Shelepova, O., Vinogradova, Yu.2016. Oil Composition of the Seeds of Echinocystis lobata (Mich.) Torr. et Gray. International Symposium on Natural Products for the Future. Tokushima, Bunri University, p. 110.

Sokolov, P. D. 1986. Rastitel'nye resursy SSSR [Plant Resources of the USSR]. Leningrad: Nauka. 336 p.

Stachowiak, D., Polanowski, A., Grazyna, B., Wilusz, T. 1996. Isolation and amino-acid sequence of two inhibitors of serine proteinases, members of the squash inhibitor family, from Echinocystis lobata seeds. Acta Biochim Pol., vol. 43, no. 3, p. 507-514. DOI:

Szot, I., Zhurba, M., Klymenko, S. 2020. Pro-health and functional properties of goji berry (Lycium spp.). Agrobiodiversity for Improving Nutrition, Health and Life Quality, vol. 4, p. 134-145. DOI:

Touihri, I., Kallech-Ziri, O., Boulila, A., Fantasi, S., Marrakchi, N., Luis, J., Belgacem, N. 2019. Ecballium elaterium (L.) A. Rich. seed oil: Chemical composition and antiproliferative effect on human colonic adenocarcinoma and fibrosarcoma cancer cell lines. Arabian Journal of Chemistry, vol. 12, no. 8, p. 2347-2355. DOI:

Vinogradova, Y. K. 2006. Etapy formirovaniya vtorichnogo areala i izmenchivost' invazionnyh populyacij ekhinocistisa shipovatogo [Stages of secondary range formation and variability of invasive populations of Echinocystis]. Bulleten Glavnogo botan. sada, vol. 192, p. 8-23. (In Russian)

Vinogradova, Y. K., Mayorov, S. R., Choroon, L. V. 2010. Black book of flora of the Middle Russia. Мoscow, Russia: Geos Press, 512 p.

Vinogradova, Y., Vergun, O., Grygorieva, O., Ivanišová, E., Brindza, J. 2020. Comparative Analysis of Antioxidant Activity and Phenolic Compounds in the Fruits of Aronia spp. Potravinarstvo Slovak Journal of Food Sciences, vol. 14, p. 393-401. DOI:



How to Cite

Vinogradova, Y., Shelepova, O., Vergun, O., Grygorieva, O., & Brindza, J. (2021). Phenolic content and antioxidant activity of Echinocystis lobata (Mich.) Torr. ET Gray (Cucurbitaceae). Potravinarstvo Slovak Journal of Food Sciences, 15, 784–791.

Most read articles by the same author(s)

1 2 3 4 > >>