Variation of fruits morphometric parameters and bioactive compounds of Asimina triloba (L.) Dunal germplasm collection


  • Ján Brindza Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Biodiversity Conservation and Biosafety, Trieda Andreja Hlinku 2, 949 76 Nitra
  • Olga Grygorieva M. M. Gryshko National Botanical Gardens of Ukraine, National Academy of Sciences, Timiryazevska 1, 01014 Kyiv
  • Svitlana Klymenko M. M. Gryshko National Botanical Gardens of Ukraine National Academy of Sciences, Timiryazevska 1, 01014 Kyiv
  • Olena Vergun M. M. Gryshko National Botanical Gardens of Ukraine National Academy of Sciences, Timiryazevska 1, 01014 Kyiv
  • Ján Mareček Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Plant Storage and Processing, Trieda Andreja Hlinku 2, 949 76 Nitra
  • Eva Ivanišová Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Resources, Department of Plant Storage and Processing, Trieda Andreja Hlinku 2, 949 76 Nitra



Asimina triloba, Dunal, fruits, seeds, variability, bioactive compounds


The objective of this study was to evaluate the morphological parameters and bioactive compounds (antioxidant activity, total polyphenol, flavonoid, and phenolic acid content) of 6 genotypes of dry Asimina triloba (L.) fruit from Slovak University of Agriculture in Nitra (Slovakia). Genotypes were obtained from the seeds that were sown in the year 2000. Their morphometric parameters were following: fruit weight from 59.00 to 241.19 g, fruit length from 50.14 to 140.11 mm, fruit diameter from 37.55 to 64.67 mm, number of fruits per cluster from 2 to 8, seed weight from 0.06 to 1.80 g, seed length from 16.33 to 29.11 mm, seed width from 9.56 to 18.33 mm, seed thickness from 4.98 to 9.75 mm, number of seeds in fruit from 4 to 16. The shape indexes of fruits were found ranging from 1.53 to 2.16. The variability of important is the average seeds weight from 7.40 to 35.61%, fruit weight from 14.84 to 32.95%, number of fruits per cluster from 18.21 to 32.54% and a number of seeds in fruit from 13.49 to 27.72%. The other characteristics are more or less stable. Total polyphenol content ranged from 22.13 to 37.36 mg GAE per g, total flavonoid content from 15.10 to 32.03 mg QE per g and phenolic acids content from 0.23 to 0.76 mg CAE per g. All tested samples exhibited DPPH radical scavenging activities with values from 2.84 to 7.04 mg TEAC per g. Antioxidant activity by molybdenum reducing antioxidant power method ranged from 97.25 to 275.41 mg TEAC per g of dry matter. Differences between the genotypes were significant in all observed parameters. This species is potential for propagation and practice used in the Slovak Republic.


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Alali, F. Q., Liu, X. X., McLaughlin, J. L. 1999. Annonaceous acetogenins: recent progress. J. Nat. Prod., vol. 62, no. 3, p. 504-540. DOI:

Brannan, R. G., Peters, T., Talcott, S. T. 2014. Phytochemical Analysis of ten varieties of pawpaw (Asimina triloba (L.) Dunal) fruit pulp. Food Chemistry, vol. 168, p. 656-661. DOI:

Brannan, R. G., Salabak, D. E., Holben, D. H. 2012. Sensory analysis of pawpaw (Asimina triloba) pulp puree: Consumer appraisal and descriptive lexicon. Journal of Food Research, vol. 1, no. 1, p. 179-192. DOI:

Brindza, P., Brindza, J., Tóth, D., Klimenko, S. V., Grigorieva, O. 2007. Slovakian cornelian cherry (Cornus mas L.) : potential for cultivation. Acta Hortic., vol. 760, p. 433-437. DOI:

Crabtree, S. B. 2004. Sexual and asexual reproductive characteristics of the north American pawpaw (Asimina triloba (L.) Dunal) : dissertation theses. Lexington, Kentucky : University of Kentucky. 80 p.

Cuendet, M., Oteham, C. P., Moon, R. C., Keller, W. J., Peaden, P. A., Pezzuto, J. M. 2008. Dietary Administration of Asimina triloba. (Paw Paw) extract increases tumor latency in n.-methyl-n.-nitrosourea–treated rats. Pharmaceutical Biology, vol. 46, no. 1-2, p. 3-7. DOI:

Donno, D., Beccaro, G. L., Mellano, M. G., Cerutti, A. K., Bounous, G. 2014. Chemical fingerprinting as nutraceutical quality differentiation tool in Asimina triloba L. fruit pulp at different ripening stages: an old species for new health needs. Journal of Food and Nutrition Research, vol. 53, no. 1, p. 81-95.

Farag, M. A. 2009. Chemical composition and biological activities of Asimina triloba leaf essential oil. Pharmaceutical Biology, vol. 47, no. 10, p. 982-986. DOI:

Farmakopea Polska, 1999. The Polish Farmaceutical Society [online] s.a. [cit.2017-01-25] Available at:

Galli, F., Archbold, D. D., Pomper, K. W. 2007. Pawpaw: An old fruit for new needs. Acta Horticulturae, vol. 744, p. 461-466. DOI:

Goodrich, K. R., Zjhra, M. L., Ley, C. A., Raguso, R. A. 2006. When flowers smell fermented: the chemistry and ontogeny of yeasty floral scent in pawpaw (Asimina triloba: Annonaceae). Int. J. Plant Sci., vol. 167, no. 1, p. 33-46. DOI:

Grygorieva, O., Abrahamová, V., Karnatovská, M., Bleha, R., Brindza, J. 2014. Morphological characteristic of fruit, drupes and seeds genotypes of Ziziphus jujuba Mill. Potravinarstvo, vol. 8, no. 1, p. 306-314. DOI:

Gu, Z. M., Zhou, D., Lewis, N. J., Wu, J., Johnson, H. A., McLaughlin, J. L., Gordon, J. 1999. Quantitative evaluation of Annonaceous acetogenins in monthly samples of paw paw (Asimina triloba) twigs by liquid chromatography/electrospray ionization/tandem mass spectrometry. Phytochemical Analysis, vol. 10, no. 1, p. 32-38.;2-D DOI:<32::AID-PCA433>3.0.CO;2-D

Harris, G. G., Brannan, R. G. 2009. A preliminary evaluation of antioxidant compounds, reducing potential, and radical scavenging of pawpaw (Asimina tribloba) fruit pulp from different stages of ripeness. Lwt-Food Science and Technology, vol. 42, no. 1, p. 275-279. DOI:

Horčinová Sedláčková, V., Grygorieva, O., Fatrcová-Šramková, K., Vergun, O., Vinogradova, Y., Ivanišová, E., Brindza, J. 2018. The morphological and antioxidant characteristics of inflorescences within wild-growing genotypes of elderberry (Sambucus nigra L.). Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, p. 444-453. DOI:

Hui, Y. H., Rupprecht, J. K., Liu, Y. M., Anderson, J. E., Smith, D. L., Chang, C. J., McLaughlin, J. L. 1989. Bullatacin and bullatacinone: two highly potent bioactive acetogenins from Annona bullata. J. Nat. Prod., vol. 52, no. 3, p. 463-477. DOI:

Ivanišová, E., Grygorieva, O., Abrahamová, V., Schubertova, Z., Terentjeva, M., Brindza, J. 2017. Characterization of morphological parameters and biological activity of jujube fruit (Ziziphus jujube Mill.). Journal of Berry Research, vol. 7, no. 4, p. 249-260. DOI:

Jones, L., Layne, D. R., Kwantes, M. G. 1995. Pawpaw (Asimina triloba) seedling growth and development: Influence of incident light intensity and fertilization regime. HortScience, vol. 30, no. 3, p. 437. DOI:

Klymenko, S., Grygorieva, O., Brindza, J. 2017. Less Known Species of Fruit Crops. Slovakia, Nitra : SPU, 104 p. DOI:

Ko, Y., Wu, T., Wu, Y., Chang, F., Guh, J., Chuang, L. 2011. Annonacin induces cell cycle-dependent growth arrest and apoptosis in estrogen receptor-α-related pathways in MCF-7 cells. J. Ethnopharmacol., vol. 137, no. 3, p. 1283-1290. DOI:

Kobayashi, H., Wang, C., Pomper, K. W. 2008. Phenolic content and antioxidant capacity of pawpaw fruit (Asimina triloba L.) at different ripening stages. HortScience, vol. 43, no. 1, p. 268-270. DOI:

Layne, D. R. 1996. The pawpaw (Asimina triloba (L.) Dunal): a new fruit crop for Kentucky and the United States. HortScience, vol. 31, no. 5, p.775-784. DOI:

United States Patent and Trademark Office. Chemotherapeutically active acetogenins. Pattent owner: McLaughlin, J. L., Hui, Y. H. United States. Patent no. 5229419.

Monka, A., Grygorieva, O., Chlebo, P., Brindza, J. 2014. Morphological and antioxidant characteristics of quince (Cydonia oblonga Mill.) and chinese quince fruit (Pseudocydonia sinensis Schneid). Potravinarstvo, vol. 8, no. 1, p. 333-340. DOI:

Nam, J-S., Jang, H-L., Rhee, Y H. 2017. Antioxidant activities and phenolic compounds of several tissues of pawpaw (Asimina triloba (L.) Dunal) grown in Korea. Journal of Food Science, vol. 82, no. 8, p. 1827-1833. DOI:

Pande, G., Akoh, C. C. 2010. Organic acids, antioxidant capacity, phenolic content and lipid characterisation of Georgia-grown underutilized fruit crops. Food Chem., vol. 120, no. 2, p. 1067-1075. DOI:

Pomper, K. W., Layne, D. R. 2005. The North American pawpaw: botany and horticulture. Hortic Rev., vol. 31, p. 351-384. DOI:

Prieto, P., Pinera, M., Aguilar, M. 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry, vol. 269, no. 2, p. 334-337. DOI:

Ratnayake, S., Rupprecht, J. K., Potter, W. M., McLaughlin, J. L. 1992. Evaluation of various parts of the paw paw tree, Asimina triloba (Annonaceae), as commercial sources of the pesticidal annonaceous acetogenins. J. Econ. Entomol., vol. 85, no. 6, p. 2353-2356. DOI:

Rupprecht, J. K., Chang, C.-J., Cassady, J. M., McLauglin, J. L. 1986. Asimicin, a new cytotoxic and pesticidal acetogenin from the pawpaw, Asimina triloba (Annonaceae). Heterocycles, vol. 24, no. 5, p.1197-1201. DOI:

Sanches-Moreno, C., Larrauri, A., Saura-Calixto, F. 1998. A procedure to measure the antioxidant efficiency of polyphenols. Journal of Sciences and Food Agricultural, vol. 76, no. 2, p. 270-276.<270::AID-JSFA945>3.0.CO;2-9 DOI:<270::AID-JSFA945>3.0.CO;2-9

Sedlacek, J. D., Friley, K. L., Lowe, J. D., Pomper, K. W. 2010. Potential of ripe pawpaw fruit extract as an insecticide and feeding deterrent for striped cucumber beetle (Coleoptera: Chrysomelidae) on Squash. Journal of Entomological Science, vol. 45, no. 4, p. 378-384. DOI:

Shafii, Z. A., Basri, M., Malek, E. A., Ismail, M. 2017. Phytochemical and antioxidant properties of Manilkara zapota (L.) P roen fruit extracts and its formulations for cosmceuetical application. Asian Journal of Plant Science and Research, vol. 7, no. 3, p. 29-41.

Sica, V. P., El-Elimata, T., Oberlies, N. H. 2016. In situ analysis of Asimina triloba (paw paw) plant tissues for acetogenins via the droplet-liquid microjunction-surface sampling probe coupled to UHPLC-PDA-HRMS/MS. Analytical methods : advancing methods and applications, vol. 8, no. 32, p. 6143-6149. DOI:

Singleton, V. L., Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Agricultural, vol. 6, p. 144-158.

Stehlíková, B. 1998. Basics of biostatiscics (Biodiversity protection 51). Nitra, Slovakia : SPU, 79 p. ISBN 80-7137-539-X.

Szilagyi, B. A., Stănică, F., Dănăilă-Guidea, S. M. 2016. Study the morphology of Asimina triloba (L.) Dunal fruits and seeds obtained in the Transylvania region of Romania. Current Trends in Natural Sciences, vol. 5, no. 9, p. 79-83.

Templeton, S. B., Marlette, M., Pomper, K. W., Jones, S. C. 2003. Favorable taste ratings for several pawpaw products. Hort Technol., vol. 13, p. 445-448. DOI:

Vinogradova, Y., Grygorieva, O., Vergun, O., Brindza, J. 2017. Morphological characteristics for fruits of Aronia mitschurinii A. K. Skvortsov & Maitul. Potravinarstvo Slovak Journal of Food Sciences, vol. 11, no. 1, p. 754-760. DOI:

Woo, M. H., Zeng, L., Ye, Q., Gu, Z., Zhao, G., McLaughlin, J. L. 1995. 16, 19-cis-Murisolin and murisolin A, two novel bioactive mono-tetrahydrofuran annonaceous acetogenins from Asimina triloba seeds. Bioorg Med Chem Lett., vol. 5, no. 11, p. 1135-1140. DOI:

Zhao, G., Hui, Y., Rupprecht, J. K., McLaughlin, J. L., Wood, K. V. 1992. Additional bioactive compounds and trilobacin, a novel highly cytotoxic acetogenin, from the bark of Asimina triloba. J. Nat. Prod., vol. 55, no. 3, p. 347-356. DOI:

Zhao, G. X., Miesbauer, L. R., Smith, D. L., McLaughlin, J. L. 1994. Asimin, asiminacin, and asiminecin: Novel highly cytotoxic asimicin isomers from Asimina triloba. J. Med. Chem., vol. 37, no. 13, p. 1971-1976. DOI:

Zhao, G. X., Rieser, M. J., Hui, Y. H., Mies bauer, L. R., Smith, D. L., McLaughlin, J. L. 1993. Biologically-active acetogenins from stem bark of Asimina triloba. Phytochemistry, vol. 33, no. 5, p. 1065-1073. DOI:



How to Cite

Brindza, J. ., Grygorieva, O. ., Klymenko, S. ., Vergun, O. ., Mareček, J. ., & Ivanišová, E. . (2019). Variation of fruits morphometric parameters and bioactive compounds of Asimina triloba (L.) Dunal germplasm collection. Potravinarstvo Slovak Journal of Food Sciences, 13(1), 1–7.

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