Antibiotic resistance in bacteria Staphylococcus spp. isolated from samples of raw sheep's milk


  • Milan Vasiľ University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81, Košice
  • Juraj Elečko University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81, Košice
  • Zuzana Farkašová University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81, Košice
  • František Zigo University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81, Košice
  • Martin Lapin University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenského 73, 041 81, Košice



sheep, raw milk, antibiotic, resistance, Staphylococcus spp.


From samples of raw sheep's milk were determined results of bacteriological examination from two herds in region of Eastern Slovakia in three years lasting study. The occurrence of Staphylococcus spp. 41.6% (124) was determined from 298 samples. The seven species of staphylococci were on a regular basis isolated: S. epidermidis (34), S. chromogenes (26), S. aureus (16). Alternately have been recorded S. warneri (16), S. schleiferi (15), S. haemolyticus (9) and S. xylosus (8). All isolated pathogens were tested by in vitro test on Mueller-Hinton agar by disc methods on resistance to 10 types of antibiotics.  Highest value of resistance was determined to Penicilin 21.0%, Neomycin 10.5% and Novobiocin 9.7%. Lower resistance was in to Oxacilin 7.2% and Amoxicilin 6.5%. Minimal resistance was founded to Cefoxitin 0.8%, Linkomycin 2.4%, Erytromycin, and Streptomycin 3.2%. Was founded total resistance (21.0%) to all antibiotics in S. epidermidis (34) during the three years, S. chromogenes (26) showed resistance to 8 types of antibiotics (12.9%), S. aureus (16) to 6 antibiotics (10.5%) and S. warneri (16) to 4 antibiotics (5.6%). It was confirmed that sheep's milk remains a major source of staphylococci. Bacteria in comparison with isolates from cows' raw milk, showed lower values of resistance, but were resistant to more than two antibiotics. Recorded occurrence of resistance in staphylococci may be connected with a minimum use of antibiotics in the treatment of mastitis and other diseases in sheep herds. Reported resistance to the tested antibiotics became the basis for the recommendation to use preparations to treat mastitis in sheep principally by the detection of resistance to antibiotics contained.


Download data is not yet available.


Metrics Loading ...


Baskaran, S. A., Kazmer, G. W., Hinckley, L., Andrew, S. M., Venkitanarayanan, K. 2009. Antibacterial effect of plant-derived antimicrobials on major bacterial mastitis pathogens in vitro. Journal of Dairy Science, vol. 92, no. 4, p. 1423-1429. DOI:

Bengtsson, B., Unnerstad, H. E., Ekman, T., Artursson, K., Nilsson-Ost, M., Waller, K. P. 2009. Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows. Veterinary Microbiology, vol. 136, no. 1-2, p. 142-149. DOI:


Bennedsgaard, T. W., Thamsborg, S. M., Aarestrup, F. M., Enevoldsen, C., Vaarst, M., Christoffersen, A. B. 2006. Resistance to penicillin of Staphylococcus aureus isolated from cows with high somatic cell counts in organic and conventional dairy herds in Denmark. Acta Veterinaria Scandinavica, vol. 48, p. 24. DOI:

Bergonier, D., Berthelot, X. 2003. New advances in epizootiology and control of ewe mastitis. Livestock Production Science, vol. 79, no. 1, p. 1-16. DOI:

Bergonier, D., Crémoux, R., Rupp, R., Lagriffoul, G. Erthelot, X. 2003. Mastitis of dairy small ruminants. Veterinary Research, vol. 34, no. 5, p. 689-716. DOI:

Berthelot, X., Lagriffoul, G., Concordet, D., Barillet, F., Bergonier, D. 2006. Physiological and pathological thresholds of somatic cell counts in ewe milk. Small Ruminant Research. vol. 62, no. 1-2, p. 27-31. DOI:

Bíreš, J., Húska, M., Vasiľ, M. 2009. Antimikrobiálna rezistencia-aktuálny problém vo veterinárnej medicíne a vo verejnom zdraví, (Antimicrobial resistance - current problem in veterinary medicine and public health). Potravinarstvo, vol. 3, no. 2, p. 1-3. Available online:

Ebrahimi, A., Lotfalian, Sh., Karimi, S. 2007. Drug resistance in isolated bacteria from milk of sheep and goats with subclinical mastitis in Shahrekord district. Iranian Journal of Veterinary Research, vol. 8, no. 1, ser. no. 18, p. 76-79.

EUCAST: European Society of Clinical Microbiology and Infectious Diseases. 2015. Disk Diffusion Method for Antimicrobial Susceptibility Testing - Version 5.0 (January 2015). Available online:

Fagundes, H., Barches, L., Filho N. A., Menezes Ferreira, M. L., Fernandes Oliveira C. A. 2010. Occurrence of Staphylococcus aureus in raw milk produced in dairy farms in Sao Paulo Stat, Brazil. Brazilian Journal of Microbiology, vol. 41, no. 2, p. 376-380. DOI:

Franca, C. A., Peixoto, R. M., Cavalcante, M. B., Melo, N. F., Oliveira, C. J. B., Veschi, J. L. A., Mota, R. A., Costa M. M. 2012. Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil. Pesquisa Veterinária Brasileira, vol. 32, no. 8, p. 747-753. DOI:

Gelasakis, A. I., Mavrogianni, V. S., Petridis, I. G., Vasileiou, N. G. C., Fthenakis, G. C. 2015. Mastitis in sheep -The last 10 years and the future of research. Veterinary Microbiology, vol. 101, p. 136-146. PMid:26216457 DOI:

Gentilini, E., Denamiel, G., Betancor, A., Revuelto, M., Rodriguez, F. M., De Torrest, R. A. 2002. Antimicrobial susceptibility of coagulase negative staphylococci isolated from bovine mastitis in Argentina. Journal of Dairy. Science, vol. 85, no. 8, p. 1913-1917. DOI:

Hariharan, H., Donachie, W., Macaldowie, C., Keefe, G. 2004. Bacteriology and somatic cell counts in milk samples from ewes on a Scottish farm. Canadian Journal of Veterinary Research, vol. 68, no. 3, p. 188-92. PMid:15352543

Hleba, L., Kačniová, M., Čuboň, J., Haščik, P., Pochop, J., Kunová, S., Mihok, M. 2010. Antimikrobiálna rezistencia -aktuálny problem vo veterinárnej medicíne a vo verejnom zdraví, (Antibiotic resistance of enterobacteriaceae genera isolated from sheep milk products). Potravinarstvo, vol. 4, special no., p. 158-165. Available online:

Chaffer, M., Leitner, G., Zamir, S., Winkler, M., Glickman, A., Ziv, N. Saran, A. 2003. Efficacy of dry-off treatment in sheep. Small Ruminant Research, vol. 47, no. 1, p. 11-16. DOI:

Kirkan, S., Goksoy, E. O., Kaya, O. 2005. Identification and antimicrobial susceptibility of Staphylococcus aureus and coagulase negative staphylococci from bovine mastitis in the Aydin region of Turkey. Turkish Journal of Veterinary and Animal Science, vol. 29, p. 791-796.

McKinnon, C. H., Rowlands, G. J., Bramley, A. J. 1990. The effect of udder preparation before milking and contamination from the milking plant on bacterial numbers in bulk milk of eight dairy herds. Journal of Dairy Research, vol. 57, no. 3, p. 307-318. DOI:

Nováková, I., Kačániová, M., Haščík, P., Pavličová, S., Hleba, L. 2009. The resistant to antibiotics in strains E. coli and Enterococcus sp. isolated from rectal swabs of lambs and calves, Lucraristiintifice Zootehniesi Biotehnologii, vol. 42, no. 2, p. 322-326.

Onni, T., Sanna, G., Larsen, J., Tola, S. 2011. Antimicrobial susceptibilities and population structure of Staphylococcus epidermidis associated with ovine mastitis. Veterinary Microbiology, vol. 148, no. 1, p. 45-50. DOI:

Pyörälä, S., Taponen, S. 2009. Coagulase-negative staphylococci - Emerging mastitis pathogens. Veterinary Microbiology, vol. 134, no. 1-2, p. 3-8. DOI:

Skovgaard, N. 2007. New trends in emerging pathogens. International Journal of Food Microbiology, vol. 120, no. 3, p. 216-219. DOI:

Tel, O. Y., Aslantaş, O., Keskin, O., Yilmaz. E. S. Demir, C. 2012. Investigation of the antibiotic resistance and biofilm formation of Staphylococcus aureus strains isolated from gangrenous mastitis of ewes. Acta Veterinaria Hungarica, vol. 60, no. 2, p. 189-197. PMid:22609990 DOI:

Taponen, S., Pyorälä, S. 2009. Coagulase-negative staphylococci as cause of bovine mastitis-Not so different from Staphylococcus aureus? Veterinary Microbiology, vol. 134, no. 1-2, p. 29-36. PMid:18977615 DOI:

Vasiľ, M., Elečko, J., Farkašová, Z., Bíreš, J. 2009. The reduction on the occurrence of mastitis in dairy herd using the innovation of housing conditions, sanitary of milk storage and applying the therapy of mastitis during the lactation. Folia Veterinaria, vol. 53, suppl. II, p. 186-189.

Virdis, S., Scarano, Ch., Cossu, F., Spanu, V., Spanu, C., De Santis, E. P. L. 2010. Antibiotic resistance in Staphylococcus aureus and coagulase negative Staphylococci isolated from goats with subclinical mastitis. Veterinary Medicine International, p. 1-6. DOI:

Witte, W. 2000. Selective pressure by antibiotic use in livestock. Internet Journal of Antimicrobial Agents, vol. 16, suppl. 1, p. 19-24. DOI:




How to Cite

Vasiľ, M. ., Elečko, J. ., Farkašová, Z. ., Zigo, F. ., & Lapin, M. . (2016). Antibiotic resistance in bacteria Staphylococcus spp. isolated from samples of raw sheep’s milk. Potravinarstvo Slovak Journal of Food Sciences, 10(1), 619–624.

Most read articles by the same author(s)

1 2 > >> 

Similar Articles

You may also start an advanced similarity search for this article.