Antibiotic resistance in bacteria Staphylococcus spp. isolated from samples of raw sheep's milk
DOI:
https://doi.org/10.5219/680Keywords:
sheep, raw milk, antibiotic, resistance, Staphylococcus spp.Abstract
From samples of raw sheep's milk were determined results of bacteriological examination from two herds in region of Eastern Slovakia in three years lasting study. The occurrence of Staphylococcus spp. 41.6% (124) was determined from 298 samples. The seven species of staphylococci were on a regular basis isolated: S. epidermidis (34), S. chromogenes (26), S. aureus (16). Alternately have been recorded S. warneri (16), S. schleiferi (15), S. haemolyticus (9) and S. xylosus (8). All isolated pathogens were tested by in vitro test on Mueller-Hinton agar by disc methods on resistance to 10 types of antibiotics. Highest value of resistance was determined to Penicilin 21.0%, Neomycin 10.5% and Novobiocin 9.7%. Lower resistance was in to Oxacilin 7.2% and Amoxicilin 6.5%. Minimal resistance was founded to Cefoxitin 0.8%, Linkomycin 2.4%, Erytromycin, and Streptomycin 3.2%. Was founded total resistance (21.0%) to all antibiotics in S. epidermidis (34) during the three years, S. chromogenes (26) showed resistance to 8 types of antibiotics (12.9%), S. aureus (16) to 6 antibiotics (10.5%) and S. warneri (16) to 4 antibiotics (5.6%). It was confirmed that sheep's milk remains a major source of staphylococci. Bacteria in comparison with isolates from cows' raw milk, showed lower values of resistance, but were resistant to more than two antibiotics. Recorded occurrence of resistance in staphylococci may be connected with a minimum use of antibiotics in the treatment of mastitis and other diseases in sheep herds. Reported resistance to the tested antibiotics became the basis for the recommendation to use preparations to treat mastitis in sheep principally by the detection of resistance to antibiotics contained.
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Baskaran, S. A., Kazmer, G. W., Hinckley, L., Andrew, S. M., Venkitanarayanan, K. 2009. Antibacterial effect of plant-derived antimicrobials on major bacterial mastitis pathogens in vitro. Journal of Dairy Science, vol. 92, no. 4, p. 1423-1429. https://doi.org/10.3168/jds.2008-1384 DOI: https://doi.org/10.3168/jds.2008-1384
Bengtsson, B., Unnerstad, H. E., Ekman, T., Artursson, K., Nilsson-Ost, M., Waller, K. P. 2009. Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows. Veterinary Microbiology, vol. 136, no. 1-2, p. 142-149. https://doi.org/10.1016/j.vetmic.2008.10.024 DOI: https://doi.org/10.1016/j.vetmic.2008.10.024
PMid:19058930
Bennedsgaard, T. W., Thamsborg, S. M., Aarestrup, F. M., Enevoldsen, C., Vaarst, M., Christoffersen, A. B. 2006. Resistance to penicillin of Staphylococcus aureus isolated from cows with high somatic cell counts in organic and conventional dairy herds in Denmark. Acta Veterinaria Scandinavica, vol. 48, p. 24. DOI: https://doi.org/10.1186/1751-0147-48-24
Bergonier, D., Berthelot, X. 2003. New advances in epizootiology and control of ewe mastitis. Livestock Production Science, vol. 79, no. 1, p. 1-16. https://doi.org/10.1016/S0301-6226(02)00145-8 DOI: https://doi.org/10.1016/S0301-6226(02)00145-8
Bergonier, D., Crémoux, R., Rupp, R., Lagriffoul, G. Erthelot, X. 2003. Mastitis of dairy small ruminants. Veterinary Research, vol. 34, no. 5, p. 689-716. https://doi.org/10.1051/vetres:2003030 DOI: https://doi.org/10.1051/vetres:2003030
Berthelot, X., Lagriffoul, G., Concordet, D., Barillet, F., Bergonier, D. 2006. Physiological and pathological thresholds of somatic cell counts in ewe milk. Small Ruminant Research. vol. 62, no. 1-2, p. 27-31. https://doi.org/10.1016/j.smallrumres.2005.07.047 DOI: https://doi.org/10.1016/j.smallrumres.2005.07.047
Bíreš, J., Húska, M., Vasiľ, M. 2009. Antimikrobiálna rezistencia-aktuálny problém vo veterinárnej medicíne a vo verejnom zdraví, (Antimicrobial resistance - current problem in veterinary medicine and public health). Potravinarstvo, vol. 3, no. 2, p. 1-3. Available online: http://www.potravinarstvo.com/dokumenty/potravinarstvo_no2_2009.pdf
Ebrahimi, A., Lotfalian, Sh., Karimi, S. 2007. Drug resistance in isolated bacteria from milk of sheep and goats with subclinical mastitis in Shahrekord district. Iranian Journal of Veterinary Research, vol. 8, no. 1, ser. no. 18, p. 76-79.
EUCAST: European Society of Clinical Microbiology and Infectious Diseases. 2015. Disk Diffusion Method for Antimicrobial Susceptibility Testing - Version 5.0 (January 2015). Available online: http://www.eucast.org
Fagundes, H., Barches, L., Filho N. A., Menezes Ferreira, M. L., Fernandes Oliveira C. A. 2010. Occurrence of Staphylococcus aureus in raw milk produced in dairy farms in Sao Paulo Stat, Brazil. Brazilian Journal of Microbiology, vol. 41, no. 2, p. 376-380. https://doi.org/10.1590/S1517-83822010000200018 DOI: https://doi.org/10.1590/S1517-83822010000200018
Franca, C. A., Peixoto, R. M., Cavalcante, M. B., Melo, N. F., Oliveira, C. J. B., Veschi, J. L. A., Mota, R. A., Costa M. M. 2012. Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil. Pesquisa Veterinária Brasileira, vol. 32, no. 8, p. 747-753. https://doi.org/10.1590/S0100-736X2012000800012 DOI: https://doi.org/10.1590/S0100-736X2012000800012
Gelasakis, A. I., Mavrogianni, V. S., Petridis, I. G., Vasileiou, N. G. C., Fthenakis, G. C. 2015. Mastitis in sheep -The last 10 years and the future of research. Veterinary Microbiology, vol. 101, p. 136-146. https://doi.org/10.1016/j.vetmic.2015.07.009 PMid:26216457 DOI: https://doi.org/10.1016/j.vetmic.2015.07.009
Gentilini, E., Denamiel, G., Betancor, A., Revuelto, M., Rodriguez, F. M., De Torrest, R. A. 2002. Antimicrobial susceptibility of coagulase negative staphylococci isolated from bovine mastitis in Argentina. Journal of Dairy. Science, vol. 85, no. 8, p. 1913-1917. https://doi.org/10.3168/jds.S0022-0302(02)74267-7 DOI: https://doi.org/10.3168/jds.S0022-0302(02)74267-7
Hariharan, H., Donachie, W., Macaldowie, C., Keefe, G. 2004. Bacteriology and somatic cell counts in milk samples from ewes on a Scottish farm. Canadian Journal of Veterinary Research, vol. 68, no. 3, p. 188-92. PMid:15352543
Hleba, L., Kačniová, M., Čuboň, J., Haščik, P., Pochop, J., Kunová, S., Mihok, M. 2010. Antimikrobiálna rezistencia -aktuálny problem vo veterinárnej medicíne a vo verejnom zdraví, (Antibiotic resistance of enterobacteriaceae genera isolated from sheep milk products). Potravinarstvo, vol. 4, special no., p. 158-165. Available online: http://www.potravinarstvo.com/dokumenty/mc_februar_2010/pdf/2/Hleba.pdf
Chaffer, M., Leitner, G., Zamir, S., Winkler, M., Glickman, A., Ziv, N. Saran, A. 2003. Efficacy of dry-off treatment in sheep. Small Ruminant Research, vol. 47, no. 1, p. 11-16. https://doi.org/10.1016/S0921-4488(02)00194-3 DOI: https://doi.org/10.1016/S0921-4488(02)00194-3
Kirkan, S., Goksoy, E. O., Kaya, O. 2005. Identification and antimicrobial susceptibility of Staphylococcus aureus and coagulase negative staphylococci from bovine mastitis in the Aydin region of Turkey. Turkish Journal of Veterinary and Animal Science, vol. 29, p. 791-796.
McKinnon, C. H., Rowlands, G. J., Bramley, A. J. 1990. The effect of udder preparation before milking and contamination from the milking plant on bacterial numbers in bulk milk of eight dairy herds. Journal of Dairy Research, vol. 57, no. 3, p. 307-318. DOI: https://doi.org/10.1017/S0022029900026959
Nováková, I., Kačániová, M., Haščík, P., Pavličová, S., Hleba, L. 2009. The resistant to antibiotics in strains E. coli and Enterococcus sp. isolated from rectal swabs of lambs and calves, Lucraristiintifice Zootehniesi Biotehnologii, vol. 42, no. 2, p. 322-326.
Onni, T., Sanna, G., Larsen, J., Tola, S. 2011. Antimicrobial susceptibilities and population structure of Staphylococcus epidermidis associated with ovine mastitis. Veterinary Microbiology, vol. 148, no. 1, p. 45-50. https://doi.org/10.1016/j.vetmic.2010.07.024 DOI: https://doi.org/10.1016/j.vetmic.2010.07.024
Pyörälä, S., Taponen, S. 2009. Coagulase-negative staphylococci - Emerging mastitis pathogens. Veterinary Microbiology, vol. 134, no. 1-2, p. 3-8. DOI: https://doi.org/10.1016/j.vetmic.2008.09.015
Skovgaard, N. 2007. New trends in emerging pathogens. International Journal of Food Microbiology, vol. 120, no. 3, p. 216-219. https://doi.org/10.1016/j.ijfoodmicro.2007.07.046 DOI: https://doi.org/10.1016/j.ijfoodmicro.2007.07.046
Tel, O. Y., Aslantaş, O., Keskin, O., Yilmaz. E. S. Demir, C. 2012. Investigation of the antibiotic resistance and biofilm formation of Staphylococcus aureus strains isolated from gangrenous mastitis of ewes. Acta Veterinaria Hungarica, vol. 60, no. 2, p. 189-197. https://doi.org/10.1556/AVet.2012.016 PMid:22609990 DOI: https://doi.org/10.1556/avet.2012.016
Taponen, S., Pyorälä, S. 2009. Coagulase-negative staphylococci as cause of bovine mastitis-Not so different from Staphylococcus aureus? Veterinary Microbiology, vol. 134, no. 1-2, p. 29-36. https://doi.org/10.1016/j.vetmic.2008.09.011 PMid:18977615 DOI: https://doi.org/10.1016/j.vetmic.2008.09.011
Vasiľ, M., Elečko, J., Farkašová, Z., Bíreš, J. 2009. The reduction on the occurrence of mastitis in dairy herd using the innovation of housing conditions, sanitary of milk storage and applying the therapy of mastitis during the lactation. Folia Veterinaria, vol. 53, suppl. II, p. 186-189.
Virdis, S., Scarano, Ch., Cossu, F., Spanu, V., Spanu, C., De Santis, E. P. L. 2010. Antibiotic resistance in Staphylococcus aureus and coagulase negative Staphylococci isolated from goats with subclinical mastitis. Veterinary Medicine International, p. 1-6. DOI: https://doi.org/10.4061/2010/517060
Witte, W. 2000. Selective pressure by antibiotic use in livestock. Internet Journal of Antimicrobial Agents, vol. 16, suppl. 1, p. 19-24. https://doi.org/10.1016/S0924-8579(00)00301-0 DOI: https://doi.org/10.1016/S0924-8579(00)00301-0
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