Occurrence of resistance to antibiotics therapy in coagulase-positive and coagulase-negative Staphylococci isolated from sheep´s milk in holding in Slovakia

Milan Vasiľ; Zuzana Farkašová; Juraj  Elečko; František Zigo

doi:10.5219/1333

Authors

Milan Vasiľ University of Veterinary Medicine and Pharmacy, Department of Animal Husbandry, UVLF, Komenského 73, 041 81 Košice, Slovakia, Tel.: +421905385671 https://orcid.org/0000-0002-3607-818X
Zuzana Farkašová ., University of Veterinary Medicine and Pharmacy, Department of Animal Husbandry, UVLF, Komenského 73 041 81, Košice, Slovakia, Tel.: +421905201646
Juraj Elečko University of Veterinary Medicine and Pharmacy, Department of Animal Husbandry, UVLF, Komenského 73, 041 81 Košice, Slovakia, Tel.: +421915986734
František Zigo University of Veterinary Medicine and Pharmacy, Department of Animal Husbandry, Komenského 73, 041 81 Košice, Slovakia, Tel.: +421908689722 https://orcid.org/0000-0002-2791-166X

DOI:

https://doi.org/10.5219/1333

Keywords:

Sheep milk, coagulase-positive staphylococci, coagulase-negative staphylococci, antibiotics resistance, mastitis

Abstract

The occurrence of bacteria Staphylococcus spp. was examined in a total of 3466 individuals and in 12 pool milk samples from 2017 to 2019. The experiment was carried out in two herds of the breed of sheep, Improved Valaska, in the Slovakia region. Eleven species of the genus Staphylococcus spp. (n = 431) were isolated and taxonomically identified. From the coagulase-positive staphylococci (CPS), S. aureus was isolated during the reporting period, however, most often in the third year (50). The incidence of S. intermedius and S. hyicus were irregular. The incidence of S. schleiferi was highest at the end of the follow-up duration. From the coagulase-negative staphylococci (CNS) (n = 158), were isolated
S. epidermidis present in 20.4% (88) and S. chromogenes 11.4 % (49), S. caprae, S. xylosus, and other species rarely occurred. S. aureus (n = 133) showed maximum resistance to erythromycin 12.0%, novobiocin 10.5%, and neomycin 9.0%. The incidence of intermedial susceptibility was observed predominantly to a penicillin (16 strains), novobiocin (11 strains), erythromycin (14 strains), oxacillin, and cloxacillin (12 strains), neomycin (11 strains), and lincomycin (9 strains). Observantly, S. schleiferi (n = 101) showed the highest resistance to novobiocin (5.9%) and erythromycin (5.0%), however, a high incidence of intermediate susceptibility to erythromycin (9), amoxicillin, novobiocin (8), ampicillin, lincomycin (7), penicillin, methicilin and cefoperazone (5 strains) can be identified as adverse. The incidence of resistant and intermediate sensitive test strains S. aureus and S. schleiferi, especially for erythromycin, novobiocin, and neomycin, which are often used to treat udder inflammation in sheep, is relatively adverse.

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