Selected properties of lactic acid bacteria isolated from raw cow's milk

Authors

  • Jana Bezeková Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Product, Tr. A. Hlinku 2, 949 76 Nitra
  • Monika Lavová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Product, Tr. A. Hlinku 2, 949 76 Nitra
  • Miroslav Kročko Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Product, Tr. A. Hlinku 2, 949 76 Nitra
  • Margita Čanigová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Product, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/177

Keywords:

lactic acid bacteria, Lactobacillus sp., PCR, acidifying activity

Abstract

For the identification of lactic acid bacteria derived from raw cow's milk, 151 colonies were isolated. The grow conditions of lactic acid bacteria were at temperature 37 °C for 3 days on MRS medium. Based on microscopical preparation, negative catalase and Gram-positive test were 81 isolates confirmed as genus Lactobacillus. Out of these, 9 isolates were evaluated for acidifying activity in UHT milk at 25 °C, 30 °C and 37 °C at regular intervals during 24 hours. The average count of NSLAB lactobacilli in raw cow's milk reached the value 1.54.104 KTJ.ml-1. It was found that all tested strains of lactobacilli did not cause significant changes of titratable acidity in milk at 25 °C and 30 °C. Only one strain significantly improved the titratable acidity of milk at 37 °C after 24 hours. The acidity reached the value from 7.5 °SH to 41.9 °SH. This strain was confirmed by PCR method as Lactobacillus helveticus.

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References

BAELE, M., VANEECHOUTTE, M., VERHELST, R., VANCANNEYT, C., DEVRIESE, L. A., HAESEBROUCK, F. 2002. Identification of Lactobacillus species using tDNA-PCR. In Journal of Microbiological Methods, vol. 50, 2002, p. 263-271. https://doi.org/10.1016/S0167-7012(02)00035-0 PMid:12031576 DOI: https://doi.org/10.1016/S0167-7012(02)00035-0

BERTIER, F., BEUVIER, E., DASEN, A., GRAPPIN, R. 2001. Origin and diversity of mesophilic lactobacilli in Comté cheese, as revealed by PCR with repetitive and species-specific primers. In International Dairy Journal, vol. 11, 2001, p. 293-305. https://doi.org/10.1016/S0958-6946(01)00059-0 DOI: https://doi.org/10.1016/S0958-6946(01)00059-0

BORGO, F., RICCI, G., MANACHINI, P. L., FORTINA, M. G. 2007. Multilocus restriction typing: A tool for studying molecular diversity within Lactobacillus helveticus of dairy origin. In International Dairy Journal, vol. 17, 2007, p. 336-342. https://doi.org/10.1016/j.idairyj.2006.03.002 DOI: https://doi.org/10.1016/j.idairyj.2006.03.002

CAGNO, R. D., QUINTO, M., CORSETTI, A., MININERVINI, F., GOBBETTI, M. 2006. Assessing the proteolytic and lipolytic activities of single strains of mesophilic lactobacilli as adjunct cultures using a Caciotta cheese model system. In International Dairy Journal, vol. 16, 2006, p. 119-130. https://doi.org/10.1016/j.idairyj.2005.01.012 DOI: https://doi.org/10.1016/j.idairyj.2005.01.012

CASCIO, A. 2010. Lactobacillus helveticus. [online]. 2010. [cit. 2010-10-26]. Retrieved from the web <http://web.mst.edu/~microbio/bio221_2005/L_helveticus.htm>.

DRÁB, V., KLEČACKÁ, J., SEDLÁČEK, I., ŠVEC, P., VILÍMKOVÁ, M. 2008. Odlišení kmenů druhu Lactobacillus helveticus od fylogeneticky příbuzných druhů Lactobacillus delbrueckii a Lactobacillus acidophilus pomocí biochemických testů a molekulárně genetických metód. In Mlékařské listy zpravodaj, vol. 108, 2008, p. 21-26.

FORTINA, M. G., RICCI, G., MORA, D., PARINI, C., MANACHINI, P. L. 2001. Specific identification of Lactobacillus helveticus by PCR with pepC, pepN and htrAtargeted primers. In FEMS Microbiology Letters, vol. 198, 2001, p. 85-89. https://doi.org/10.1111/j.1574-6968.2001.tb10623.x PMid:11325558 DOI: https://doi.org/10.1111/j.1574-6968.2001.tb10623.x

FOX, P. F., McSWEENEY, P. L. H., COGAN, T. M. GUINEE, T. P. 2004. Cheese-Chemistry, Physic and Microbiology, vol. 1 3rd Edition. Elsevier, 2004, 617 p.

GARABAL, J. I., RODRÍGUEZ- ALONSO, P., CENTENO, J. A. 2008. Characterization of lactic acid bacteria isolated from raw cows´ milk cheeses currently produced in Galicia (NW Spain). In LWT Food Science and Technology, vol. 40, 2008, p. 1452-1458. https://doi.org/10.1016/j.lwt.2007.09.004 DOI: https://doi.org/10.1016/j.lwt.2007.09.004

GÖRNER, F., VALÍK, Ľ. 2004. Aplikovaná mikrobiológia požívatín. 1. vyd. Bratislava: Malé centrum, 2004, 528 p.

HOORDE, K. V., LEUVEN, I. V., DIRINCK, P., HEYNDRICKX, M., COUDIJZER, K., VANDAMME, P., HUYS, G. 2010. Selection, application and monitoring of Lactobacillus paracasei strains as adjunct cultures in the production of Gouda-type cheeses. In International Journal of Food Microbiology, vol. 144, 2010, p. 226-235. https://doi.org/10.1016/j.ijfoodmicro.2010.05.007 PMid:21036412 DOI: https://doi.org/10.1016/j.ijfoodmicro.2010.05.007

HOORDE, K. V., VERSTRAETE, T., VANDAMME, P., HUYS, G. 2008. Diversity of lactic acid bacteria in two Flemish artisan raw milk Gouda-type cheeses. In Food Microbiology, vol. 25, 2008, p. 929-935. https://doi.org/10.1016/j.fm.2008.06.006 PMid:18721684 DOI: https://doi.org/10.1016/j.fm.2008.06.006

KAGKLI, D. M., VANCANNEYT, M., HILL, C., VANDAMME, P., COGAN, T. M. 2007. Enterococcus and Lactobacillus contamination of raw milk in a farm dairy environment. In International Journal of Food Microbiology, vol. 114, 2007, p. 243-251. https://doi.org/10.1016/j.ijfoodmicro.2006.09.016 PMid:17189657 DOI: https://doi.org/10.1016/j.ijfoodmicro.2006.09.016

KALETA, P., CALLANAN, J., O'CALLAGHAN, J., FITZERALD, G. F.,, BERESFORD, T. P., ROSS, R. P. 2009. Exploitation of the diverse insertion sequence element content of dairy Lactobacillus helveticus starters as a rapid method to identify different strains. In Journal of Microbiological Methods, vol. 79, 2009, p. 32-36. https://doi.org/10.1016/j.mimet.2009.07.019 PMid:19646489 DOI: https://doi.org/10.1016/j.mimet.2009.07.019

KHOLIF, A. M., MAHRAN, G. A., EL-NAWAWY, M. A., ISMAIL, A. A., SALEM, M. M. E., ZAKY, W. M. 2011. Evaluation of Proteolytic Activity of Some Dairy Lactobacilli. In World Journal of Dairy and Food Sciences. vol. 6, 2011, p. 21-26..

NIETO-ARRIBAS, P., POVEDA, J. M., SESENA, S., PALOP, L., CABERAS, L. 2009. Technological characterization of Lactobacillus isolates from traditional Manchego cheese for potential use as adjunct starter cultures. In Food Control, vol. 20, 2009, p. 1092-1098. https://doi.org/10.1016/j.foodcont.2009.03.001 DOI: https://doi.org/10.1016/j.foodcont.2009.03.001

SÁNCHEZ, I., SESENA, S., POVEDA, J. M., CABEZAS, L., PALOP, L. 2006. Genetic diversity, dynamic, and activity of Lactobacillus community involved in traditional processing of artisanal Manchego cheese. In International Journal of Food Microbiology, vol. 107, 2006, p. 265-273. https://doi.org/10.1016/j.ijfoodmicro.2005.10.023 DOI: https://doi.org/10.1016/j.ijfoodmicro.2005.10.023

PMid:16481060

SMETANKOVÁ, J., HLADÍKOVÁ, Z., GREIF, G., GREIFOVÁ, M. 2011. Technologické vlastnosti vybraných laktobacilov majúcich antimikrobiálnu aktivitu. In Zb. XIII. konference mladých vědeckých pracovníků, VFU Brno, 2011, p. 45-47.

SMITH, G. 2003. Dairy Procesing-Improving Quality. Woodhead Publishing, 2003, 545 p. DOI: https://doi.org/10.1201/9780203486207

STN ISO 15214. 2002. Mikrobiológia potravín a krmív. Horizontálna metóda stanovenia mezofilných baktérií mliečneho kysnutia metóda počítania kolónií kultivovaných pri 37 °C. Bratislava: SÚTN, 2002.

THAGE, B. V., BROE, M. L., PETERSEN, M. H., PETERSEN, M. A., BENNEDSEN, M. ARDÖ, Y. 2005. Aroma development in semi-hard reduced-fat cheese inoculated with Lactobacillus paracasei strains with different aminotransferase profiles. In International Dairy Journal, vol. 15, 2005, p. 795-805. https://doi.org/10.1016/j.idairyj.2004.08.026 DOI: https://doi.org/10.1016/j.idairyj.2004.08.026

TŮMA, Š., PLOCKOVÁ, M. 2007. Protektívní kultury pro výrobu polotvrdých syrů. In Mléko a sýry 2007 (sborník přednášek semináře), Praha, 2007, p. 31-36. DOI: https://doi.org/10.1556/AAlim.2007.0015

WOUTERS, J. T. M., AYAD, E. H. E., HUGENHOLTZ, J., SMIT, G. 2002. Microbes from raw milk for fermented dairy products. In International Dairy Journal, vol. 12, 2002, p. 91-109. https://doi.org/10.1016/S0958-6946(01)00151-0 DOI: https://doi.org/10.1016/S0958-6946(01)00151-0

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Published

2012-01-21

How to Cite

Bezeková, J. ., Lavová, M. ., Kročko, M. ., & Čanigová, M. . (2012). Selected properties of lactic acid bacteria isolated from raw cow’s milk. Potravinarstvo Slovak Journal of Food Sciences, 6(1), 5–9. https://doi.org/10.5219/177

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