Optimalisation of species identification of common carp (Cyprinus carpio) using SYBR® green I real-time PCRmethod

Authors

  • Pavol Bajzí­k Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Radoslav Židek Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Jozef Golian Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Ľubomí­r Belej Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Jozef Čapla Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Lenka Maršálková Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Ondrej Revák Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/154

Keywords:

species identification, SYBR® Green I. Real-Time PCR, Cyprinus carpio

Abstract

European Union Member States, together with a number of countries around the world, places great emphasis on ensuring the protection of consumers as a potential food allergic from food allergies. to inform consumers that their product may contain any of the risk of allergenic ingredients. For species identification of fish and fish products as a potential food allergens are used by many analytical methods as well as their authentication. We are in our work applied the method of SYBR® Green I. Real-Time PCR. We focused on pre-designed molecular - genetic marker of common carp (Cyprinus carpio), which comes from the mtDNA control D - loop area. We analyzed its presence in DNA isolates from the 5 kinds of freshwater fish, diluted to 10 % concentration. Results of using the optimized SYBR ® Green I Real-Time PCR method for species identification common carp (Cyprinus carpio) indicate to its suitability.

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References

ASENSIO, L., MONTERO, A., 2008. Analysis of fresh fish labeling in Spanish fish retail shops. In Food Control, vol. 19, 2008, p. 795-799. https://doi.org/10.1016/j.foodcont.2007.08.005 DOI: https://doi.org/10.1016/j.foodcont.2007.08.005

ATAMAN, C., CELIK U., REHBEIN H., 2006. Identification of some Aegean fish species by native isoelectric focusing. In European Food Research and Technology, vol. 222, 2006, p. 99-104. https://doi.org/10.1007/s00217-005-0149-0 DOI: https://doi.org/10.1007/s00217-005-0149-0

BOYE, J. I., 2010. Allergen management in the food industry. New Jersey : Wiley, 2010. 624 p. ISBN 978-0-470-22735-0. https://doi.org/10.1002/9780470644584 DOI: https://doi.org/10.1002/9780470644584

CIVERA, T., 2003. Species identification and safety of fish products. In Veterinary Research Communications, vol. 27, 2003, p. 481-489. https://doi.org/10.1023/B:VERC.0000014205.87859.ab PMid:14535455 DOI: https://doi.org/10.1023/B:VERC.0000014205.87859.ab

DIETRICH, M. A., NYNCA, J,. BILIŃSKA, B., KUBA, J., KOTULA-BALAK, M., KAROL, M., CIERESZKO, A., 2010. Identification of parvalbumin-like protein as a major protein of common carp (Cyprinus carpio L) spermatozoa which appears during final stage of spermatogenesis. In Comparative Biochemistry and Physiology, Part B, vol. 157. 2010, p. 220-227. https://doi.org/10.1016/j.cbpb.2010.06.007 PMid:20601062 DOI: https://doi.org/10.1016/j.cbpb.2010.06.007

GIL, L. A., 2007. PCR-based methods for fish and fishery products authentication. In Food Science & Technology, vol. 18, 2007, p. 558-566. https://doi.org/10.1016/j.tifs.2007.04.016 DOI: https://doi.org/10.1016/j.tifs.2007.04.016

HIRD, H. J., HOLD, G. L., CHRISHOLM, J., REECE, P., RUSSELL, V. J., BROWN, J., 2005. Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR. In European Food Research and Technology, vol. 220, 2005, p. 633-637. https://doi.org/10.1007/s00217-004-1050-y DOI: https://doi.org/10.1007/s00217-004-1050-y

HORSTKOTTE, B., REHBEIN, H., 2003. Fish species identification by means of restriction fragment length polymorphism and high performance liquid chromatography. In Journal of Food Science, vol. 68, 2003, p. 2658-2666. https://doi.org/10.1111/j.1365-2621.2003.tb05785.x DOI: https://doi.org/10.1111/j.1365-2621.2003.tb05785.x

HUBALKOVA, Z., KRALIK, P., TREMLOVA, B., 2007. Methods of gadoid fish species identification in food and their economic impact in the Czech Republic: DOI: https://doi.org/10.17221/2044-VETMED

a review. In Veterinary Medicine, vol. 52, 2007, p. 273-292.

KVASNIČKA, F., 2005. Capillary electrophoresis in food authenticity. In Journal of Separation Science. vol. 28, 2005, p. 813-825. https://doi.org/10.1002/jssc.200500054 PMid:16013809 DOI: https://doi.org/10.1002/jssc.200500054

LOCKLEY, A. K., BARDSLEY, R. G., 2000. DNA-based methods for food authentication. In Trends in Food Science and Technology, vol.11, 2000, p. 67-77. https://doi.org/10.1016/S0924-2244(00)00049-2 DOI: https://doi.org/10.1016/S0924-2244(00)00049-2

MACKIE, I. M., 1996. Authenticity of fish. P. R. Ashurt, M. J. Dennis (Eds.), In Food authentication, London: Blackie Academic and Professional, 1996, p.140-170. DOI: https://doi.org/10.1007/978-1-4613-1119-5_5

MORETTI, V. M., TURCHINI, G. M., BELLAGAMBA, F., 2003. Traceability issues in fishery and aquaculture products. In Veterinary Research Communications, vol. 27, 2003, p. 497-505. https://doi.org/10.1023/B:VERC.0000014207.01900.5c PMid:14535457 DOI: https://doi.org/10.1023/B:VERC.0000014207.01900.5c

PASCUAL, C. Y., RECHE, M., FIANDOR, A., VALBUENA, T., CUEVAS, T., MARTIN-ESTEBAN, M. M., 2008. Fish allergy in childhood. In Pediatric Allergy and Immunology, vol. 19, 2008, p. 573-579. https://doi.org/10.1111/j.1399-3038.2008.00822.x PMid:18950323 DOI: https://doi.org/10.1111/j.1399-3038.2008.00822.x

POMS, R. E., KLEIN, C. L., ANKLAM, E., 2004. Methods for allergen analysis in food: a review. In Food Additives & Contaminants, vol. 21, 2004, p. 1-31. https://doi.org/10.1080/02652030310001620423 PMid:14744677 DOI: https://doi.org/10.1080/02652030310001620423

RIMÁROVÁ, K., LOVAYOVÁ, V., 2007. Alergény a lepok v potravinách, prístupy k ich detekcii a označovaniu v EÚ. In: HYGIENA ALIMENTORUM XXVIII „Bezpečnosť a kvalita mlieka a mliečnych výrobkov". Slovakia : Štrbské Pleso - Vysoké Tatry, 2007, p. 165-169. ISBN 978-80-8077-055-6.

SICHERER, S. H., SAMPSON, H. A., 2010. Food allergy. In Journal of Allergy and Clinical Immunology, vol. 125, 2010, p. S116-S126. https://doi.org/10.1016/j.jaci.2009.08.028 PMid:20042231 DOI: https://doi.org/10.1016/j.jaci.2009.08.028

TELETCHEA, T., MAUDET, C., HÄNNI, C. 2005. Food and forensic molecular identification: update and challenges. In Trends in Biotechnology, vol. 23, 2005, p. 359-366. https://doi.org/10.1016/j.tibtech.2005.05.006 PMid:15927295 DOI: https://doi.org/10.1016/j.tibtech.2005.05.006

TROTTA, M., SCHÖNHUTH, S., PEPE, T., CORTESI, M. L., PUYET, A., BAUTISTA, J. M., 2005. A multiplex - PCR Metod for use in real - time PCR for identification of fish fillets from grouper (Epinephelus spp. and Mycteroperca spp.) and common substitute species. In Journal of Agricultural and Food Chemistry, vol. 53, 2005, p. 2039 - 2045. https://doi.org/10.1021/jf048542d PMid:15769133 DOI: https://doi.org/10.1021/jf048542d

VAN HENGEL, A. J., 2007. Food allergen detection methods and the challenge to protect food-allergic consumers. In Analytical and Bioanalytical Chemistry, vol. 389, 2007, p. 111-118. https://doi.org/10.1007/s00216-007-1353-5 PMid:17530230 DOI: https://doi.org/10.1007/s00216-007-1353-5

ŽIDEK, R., GOLIAN, J., 2008. Detection of carp (Cyprinius carpio) proteins using genetic markers. In Proteiny : Sborník příspěvků V. ročníku medzinárodní konference. Zlín : Univerzita Tomáše Bati ve Zlíne, 2008, p.235-237.

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Published

2011-07-03

How to Cite

Bajzí­k, P. ., Židek, R. ., Golian, J. ., Belej, Ľubomí­r ., Čapla, J. ., Maršálková, L. ., & Revák, O. . (2011). Optimalisation of species identification of common carp (Cyprinus carpio) using SYBR® green I real-time PCRmethod. Potravinarstvo Slovak Journal of Food Sciences, 5(3), 1–5. https://doi.org/10.5219/154

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