Comparison and assessment of leptin receptor expression by the following Origami aetheroleum study at broiler chickens COBB 500

Authors

  • Ľubica Mrázová Slovak university of Agriculture, Faculty of biotechnology and food sciences, Department of hygiene and food safety. Tr. A. Hlinku 2, 94976 Nitra
  • Radoslav Židek Slovak university of Agriculture, Faculty of biotechnology and food sciences, Department of hygiene and food safety. Tr. A. Hlinku 2, 94976 Nitra
  • Mária Angelovičová Slovak university of Agriculture, Faculty of biotechnology and food sciences, Department of hygiene and food safety. Tr. A. Hlinku 2, 94976 Nitra
  • Martin Král Slovak university of Agriculture, Faculty of biotechnology and food sciences, Department of hygiene and food safety. Tr. A. Hlinku 2, 94976 Nitra

DOI:

https://doi.org/10.5219/209

Keywords:

leptin receptor, gene expression, broiler chickens, origami aetheroleum

Abstract

The recently discovered protein, leptin, is a 16 kD protein consisting of 146 amino acids which is synthesized primarily by adipose tissue and is secreted into the bloodstream after cleavage of the 21 amino acids signal peptide. Leptin impacts feed intake, the neuroendocrine-axis, metabolism and immunological processes. Leptin was first identified as the gene product found deficient in the obese ob/ob mouse. The hypothalamus appears to be the primary site of action, since leptin receptors are located within hypothalamic areas associated with control of appetite, reproduction and growth. Using herbs and essential oils depends on their antimicrobial activity. Most plants have favorable multifunctional properties, which are the specific content of bioactive components. Some authors characterize phytogenic substance such as natural substances plant origin, which leave no residues in animal products and is not necessary to keep the trade period before slaughter animals. Analyzes suggest that the structural function of the receptor exists as a dimer constructively in the plasma membrane. Each receptor dimer pair is reversibly bound to one molecule of leptin. When bound, signaling pathways are responsible for beginning the activation receptor associated Janus kinase 2 (JAK2) and tyrosine phosphorylation of two key residues in the intracellular part of receptor. The aim of our experiment was to optimize the methodology for monitoring the expression of the leptin receptor extracellular avian model. We used samples of internal organs and abdominal fat chickens that were fed spirit and also fat and organ samples from broiler chickens from the control group. In heard tissue, spleen, liver at a relatively high concentration of total cDNA in the sample length leptin receptor extracellular fragment located in the expected quantities.

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References

AUWERX, R. S., STAELS, B. 1998. Leptin. In Lancet, vol. 351, 1998, p. 737-742. https://doi.org/10.1016/S0140-6736(97)06348-4 PMiD:9504534

CASSY, S., METAYER, S., CROCHET, S., RIDEAU, N., COLLIN, A., TESSERAUD, S. 2004. Leptin receptor in the chicken ovary: potential involvement in ovarian dysfunction of ad libitum-fed broiler breeder hens. In Reproductive Biology and Endocrinology, vol. 2, 2005, no. 72, p. 178-186. https://doi.org/10.1186/1477-7827-2-72 PMiD:15473907

HOREV, G., EINAT, P., AHARONI, T., ESHDAT, Y., FRIEDMAN-EINAT, M. 2000. Molecular cloning and properties of the chicken leptin-receptor (CLEPR) gene. In Molecular and Cellular Endocrinology, vol. 162, 2000, p. 95-106. https://doi.org/10.1016/S0303-7207(00)00205-7 PMiD:10854702

KUNOVÁ, S., HLEBA, L., HAŠČÍK, P., ČUBOŇ, J., KAČÁNIOVÁ, M. 2011. Determination of leptin expression in beef cattle blood samples used by RTQ PCR. In Journal of Microiology, Biotechology and Food Sciences, vol. 1, 2011, no.1, p. 21-38.

LEE, G. H., PROENCA, R., MONTEZ, J. M., CARROLL, K. M., DARVISHZADEH, J. G., LEE, J. I. 1996. Abnormal splicing of the leptin receptor. in diabetic mice. In Nature, vol. 379, 1996, p. 632-635. https://doi.org/10.1038/379632a0 PMiD:8628397

LIU, X., DUNN, J. C., SHARP, P. J., BOSWELL, T. 2006. Molecular cloning snd tissue distribution of a short form chicken leptin receptor mRNA. In Domestic Animal Endocrinoogy, vol. 32, 2007, p. 155-166. https://doi.org/10.1016/j.domaniend.2006.02.001 PMiD:16531001

MATARESE, G., MOSCHOS, S., MANTZOROS, C. S. 2005. Leptin in immunology. In Journal of Immunology, vol. 174, 2005, p. 3137-3142. https://doi.org/10.1097/01.med.0000177421.80858.74 PMiD:15749839

O'ROURKE L., SHEPHERD, P. R. 2002. Biphasic regulation of extracellular-signal-regulated protein kinase by leptin in macrophages: role in regulating STAT3 Ser727 phosphorylation and DNA binding. In Journal of Biochemistry, vol. 364, 2002, p. 875-879. https://doi.org/10.1042/BJ20020295 PMiD:12049654

RICHARDS, M. P., POCH, S. M. 2003. Molecular cloning and expression of the turkey leptin receptor gene. In Comparative Biochemistry and Physiology., vol. 136, 2003. p. 833-847. https://doi.org/10.1016/S1096-4959(03)00260-4 PMiD:14662306

ROSENBLUM, C. I., TOTA, M., CULLY, D., SMITH, T., COLLUM, R., QURESHI, S., HESS, J. F., PHILLIPS, M. S., HEY, P. J., VONGS, A., FONG, T. M., XU, L., CHEN, H. Y., SMITH, R. G., SCHINDLER, C., VAN DER PLOEG, L. H. 1996. Functional STAT 1 and 3 signaling by the leptin receptor (OB-R); reduced expression of the rat fatty leptin receptor in transfected cells. In Endocrinology, vol. 137, 1996, p. 5178-5181. https://doi.org/10.1210/en.137.11.5178

SCHENKEL, F. S., MILLER, S. P., YE, X., MOORE, S. S. 2005. Association of single nucleotide polymorphismus in the leptin gene with carcass and meat quality traits of beef cattle. In Journal of Animal Science, vol. 83, 2005, p. 2009-2020. PMiD:16100055

SOUKAS, A., COHEN, P., SOCCI, N. D., FRIEDMAN, J. M. 2000. Leptin-specific patterns of gene expression in white adipose tissue. In Genes and Development, vol. 14, 2000, p. 963-980. PMiD:10783168

VAISSE, C., HALASS, J. V., HORVATH, C. M., DARNELL, J. E., STOFFEL, M., FRIEDMAN, J. M. 1996. Leptin activation of Stat3 in the hypothalamus of wild-type and ob/ob mice but not db/db mice. In Nature Genetics, vol. 14, 1996, p. 95-97. https://doi.org/10.1038/ng0996-95 PMiD:8782827

TARTAGLIA, L. A., DEMBSKI, M., WWNG, X., DENG, N., CULPEPPER, J., DEVOS, R. 1995. Identification and expression cloning of a leptin receptor, OB-R. In. Cell Technology, vol. 83,1995, p. 1263-1271. https://doi.org/10.1016/0092-8674(95)90151-5 PMiD:8548812

WANG, M. Y., ZHOU, Y. T., NEWGARD, C. B., UNGER, R. H. 1996. A novel leptin receptor isoform in rat. In FEBS Letters, vol. 392, 1996, p. 87-90. https://doi.org/10.1016/0014-5793(96)00790-9 PMiD:8772180

ZHANG, Y., PROENCA, R., MAFFEI, M., BARONE, M., LEOPOLD, L., FRIEDMAN, J. M. 1994. Positional cloning of the mouse obesegene and its human homologue. In Nature Medicine, vol. 372, 1994, p. 425-432. https://doi.org/10.1038/372425a0 PMiD:7984236

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Published

2012-10-11

How to Cite

Mrázová, Ľubica ., Židek, R. ., Angelovičová, M. ., & Král, M. . (2012). Comparison and assessment of leptin receptor expression by the following Origami aetheroleum study at broiler chickens COBB 500. Potravinarstvo Slovak Journal of Food Sciences, 6(3), 16–20. https://doi.org/10.5219/209

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