Analysis of gene expression in rabbit muscle

Authors

  • Alena Gálová Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra
  • Andrea Šoltýsová Slovak Academy of Sciences, Institute of Molecular Physiology and Genetics, Vlárska 5, 833 34 Bratislava, Comenius University Bratislava, Faculty of Natural Sciences, Department of Molecular Biology, Mlynská dolina, pavilion B-2, 842 15 Bratislava 4
  • Radoslav Židek Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra
  • Ján Rafay Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra
  • Jozef Nahácky Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/342

Keywords:

rabbit meat, meat quality, microarray, gene expression, candidate gene

Abstract

 Increasing consumer knowledge of the link between diet and health has raised the demand for high quality food. Meat and meat products may be considered as irreplaceable in human nutrition. Breeding livestock to higher content of lean meat and the use of modern hybrids entails problems with the quality of meat. Analysing of livestock genomes could get us a great deal of important information, which may significantly affect the improvement process. Domestic animals are invaluable resources for study of the molecular architecture of complex traits. Although the mapping of quantitative trait loci (QTL) responsible for economically important traits in domestic animals has achieved remarkable results in recent decades, not all of the genetic variation in the complex traits has been captured because of the low density of markers used in QTL mapping studies. The genome wide association study (GWAS), which utilizes high-density single-nucleotide polymorphism (SNP), provides a new way to tackle this issue. New technologies now allow producing microarrays containing thousands of hybridization probes on a single membrane or other solid support. We used microarray analysis to study gene expression in rabbit muscle during different developmental age stages. The outputs from GeneSpring GX sotware are presented in this work. After the evaluation of gene expression in rabbits, will be selected genes of interest in relation to meat quality parameters and will be further analyzed by the available methods of molecular biology and genetics.

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References

Biesalski, H. K. 2005. Meat as a component of a healthy diet - Are there any risks or benefits if meat is avoided in the diet? Meat Science. vol. 70, no. 3, p. 509-524. https://doi.org/10.1016/j.meatsci.2004.07.017 PMid:22063749 DOI: https://doi.org/10.1016/j.meatsci.2004.07.017

Brestenský, V., Baumgartner, L., Benková, J., Botto, Ľ., Brouček, j., Buchová, B., Bulla, J., Demo, P., Foltys, V., Gallo, M., Grafenau, P., Haulík, A., Hetényi, L., Horniaková, E., Huba, J., Kica, J., Margetín, M., Mihina, Š., Mojto, J., Ochodnický, D., Petrikovič, P., Peškovičová, D., Sommer, A., Šottník, J., Vláčil, R., Tančin, V. 2002. Sprievodca chovateľa hospodárskych zvierat. Publikácie VÚŽV, vol. 5, p. 130-134. [cit. 2014-02-10] Available at: http://www.cvzv.sk/ziv/spriev.pdf

Gábor, M., Miluchová, M., Trakovická A. 2012. Molekulovo-genetická analýza kandidátskych génov jemnosti mäsa hovädzieho dobytka. Vydavateľstvo SPU v Nitre, p. 3-16.

Haegeman, A., Coopman, F., Jacobs, K., Mattheeuws, M., Van Zeveren, A., Peelman, L. 2001. Bovine melanocortin receptor 4: cDNA sequence, polymorphisms and mapping. Animal Genetics, vol. 32, no. 4, p. 189-192. PMid:11531696 DOI: https://doi.org/10.1046/j.1365-2052.2001.00750.x

Jianq, M. S., Chen, Y. S., Lai, S. J., Denq, X. S., Chen Y., Wan, J. 2008. Association between single nucleotide polymorphism of MC4R gene and carcass traits in rabbits. Yi Chuan, vol. 30, no. 12, p. 1574-1578. PMid:19073572 DOI: https://doi.org/10.3724/SP.J.1005.2008.01574

Kuang, L. D., Xie, X. H., Lei, M., Li, C. Y., Ren, Y. J., Zhen, J., Zhang, X. Y., Guo, Z. Q., Zheng, Y. C. 2012. Myosin Heavy Chain Types and Expression Levels of Myostatin and Myogenin Genes in Musle of Two Rabbit Breeds. World Rabbit Science Association, Proceedings 10th World Rabbit Congress 2012, Egypt, p. 79-82. [cit. 2014-02-20] Retreived from the web: http://world-rabbit-science.com/WRSA-Proceedings/Congress-2012-Egypt/Papers/01-Genetics/G-Kuang.pdf

Norrgard, K. 2008. Genetic variation and disease: GWAS. Nature Education, [online], vol.1, no.1, p.87 [cit. 2012-11-22]. Retreived from the web: http://www.nature.com/scitable/topicpage/genetic-variation-and-disease-gwas-682

Paredi, G., Raboni, S., Bendixen E., De Almeida, A. M., Mozzarelli, A. 2012. "Muscle to meat" molecular events and technological transformations: The proteomics insight. Journal of Proteomics, vol. 75, no. 14, p. 4275-4289. https://doi.org/10.1016/j.jprot.2012.04.011 PMid:22543183 DOI: https://doi.org/10.1016/j.jprot.2012.04.011

Snustad, D. P., Simmons, J. M. 2009. Genetika. Brno 2009, p. 480-482. ISBN 978-80-210-4852-2.

Sporer, K. R. B., Tempelman, R. J., Ernst, C. W., Reed, K. M., Velleman, S. G., Strasburg, G. M. 2011. Transcriptional profiling identifies differentially expressed genes in developing turkey skeletal muscle. BMC Genomics, vol. 12, p. 143. https://doi.org/10.1186/1471-2164-12-143 PMid:21385442 DOI: https://doi.org/10.1186/1471-2164-12-143

Xu, Z., Taylor, J. A. 2009. SNPinfo: integrating GWAS and candidate gene information into functional SNP selection for genetic association studies. Nucleic Acids Research, vol. 37, p. W600-W605. https://doi.org/10.1093/nar/gkp290 PMid:19417063 DOI: https://doi.org/10.1093/nar/gkp290

Zhang, H., Wang, Z., Wang, S., Li, H. 2012. Progress of genome wide association study in domestic animals. Journal of Animal Science and Biotechnology, vol. 3, p. 26 https://doi.org/10.1186/2049-1891-3-26 PMid:22958308 DOI: https://doi.org/10.1186/2049-1891-3-26

Zhang, W. X., Zhang, G. W., Peng, J., Lai, S. J. 2012. The Polymorphism of GHR Gene Associated with the Growth and Carcass Traits in Three Rabbit Breeds. World Rabbit Science Association, Proceedings 10th World Rabbit Congress, Egypt, p. 75- 78. [cit. 2014-02-20] Retrieved from the web: http://world-rabbit-science.com/WRSA-Proceedings/Congress-2012-Egypt/Papers/01-Genetics/G-Zhang-WX.pdf

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Published

2014-06-09

How to Cite

Gálová, A. ., Šoltýsová, A. ., Židek, R. ., Rafay, J. ., & Nahácky, J. . (2014). Analysis of gene expression in rabbit muscle. Potravinarstvo Slovak Journal of Food Sciences, 8(1), 146–149. https://doi.org/10.5219/342

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