Structural changes in the rat testis caused by diazinon and selenium

Authors

  • Michal Cabaj Slovenská poľnohospodárska univerzita v Nitre, FAPZ, Katedra veterinárskych disciplín, Tr. A. Hlinku 2, 94976 Nitra, tel.: 037 641 4503
  • Róbert Toman Slovenská poľnohospodárska univerzita v Nitre, FAPZ, Katedra veterinárskych disciplín, Tr. A. Hlinku 2, 94976 Nitra
  • Mária Adamkovičová Slovenská poľnohospodárska univerzita v Nitre, FBP, Katedra veterinárskych disciplín, Tr. A. Hlinku 2, 94976 Nitra
  • Peter Massányi Slovenská poľnohospodárska univerzita v Nitre, FAPZ, Katedra veterinárskych disciplín, Tr. A. Hlinku 2, 94976 NitraSlovenská poľnohospodárska univerzita v Nitre, FBP, Katedra veterinárskych disciplín, Tr. A. Hlinku 2, 94976 Nitra
  • Branislav Šiška Slovenská poľnohospodárska univerzita v Nitre, FBP, Katedra hygieny a bezpečnosti potravín, Tr. A. Hlinku 2, 94976 Nitra
  • Norbert Lukáč Slovenská poľnohospodárska univerzita v Nitre, FBP, Katedra veterinárskych disciplín, Tr. A. Hlinku 2, 94976 Nitra
  • Jozef Golian Slovenská poľnohospodárska univerzita v Nitre, FBP, Katedra hygieny a bezpečnosti potravín, Tr. A. Hlinku 2, 94976 Nitra

DOI:

https://doi.org/10.5219/44

Keywords:

diazinon, selenium, testis, histology, morphometry, rat

Abstract

The aim of this study was to find the structural changes in the rat testis after a diazinon, selenium and their combined administration. The testis structural changes after the diazinon intraperitoneal administration of 20 mg/kg b.w., selenium 2 mg/kg b.w. and both diazinon 20 mg/kg b.w. and selenium 2 mg/kg b.w. were evaluated by histological and morphometric methods in light microscopy. 36 hours after the diazion i.p. administration, the vacuolization of the seminiferous epithelium, evacuation of germ cells into the tubule lumen, epithelium necrosis and disintegration, interstitium extension and fibrotization were observed. The germ cells released from the basal lamina and subsequently they were visible in the tubule lumen. Blood vessels were damaged and morphometric analysis have shown their significant dilatation (P<0.05). Diazinon causes the damage of the germinal epithelium in the testes leading to the spermatogenesis failure. The infertility can then appear. In selenium treated group disintegration of cellular associations in the seminiferous epithelium, damaged and separating spermatids lines, reduced spermatogenesis and significant vacuolization of seminiferous epithelium (P<0.0001) were observed. Similar changes as in selenium threated group occured, significant vacuolization of seminiferous epithelium (P<0.05) was seen. In combined diazinon and selenium treated group protective effects of selenium in seminiferous epithelium and interstitium were noticed. Further investigation of diazinon and selenium is needed to practical use this results.

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References

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Published

2010-05-31

How to Cite

Cabaj, M. ., Toman, R. ., Adamkovičová, M. ., Massányi, P. ., Šiška, B. ., Lukáč, N. ., & Golian, J. . (2010). Structural changes in the rat testis caused by diazinon and selenium. Potravinarstvo Slovak Journal of Food Sciences, 4(2), 8–16. https://doi.org/10.5219/44

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