Evaluation and comparison of the content of total polyphenols and antioxidant activity in garlic (Allium sativum L.)
Keywords:antioxidant activity, garlic, total polyphenols
Garlic (Allium sativum L.) is one of the oldest cultivated plants in the world and highly valued throughout the ages as a culinary spice. It is a hardy perennial belonging to the Alliaceae family. The garlic bulb is the most commonly used portion of the plant, composed of 5 - 20 individual. It is a very good source of manganese, selenium, vitamin C and vitamin B6 (pyridoxine). In addition, garlic is a good source of other minerals, including phosphorous, calcium, potassium, iron and copper. Many of the perceived therapeutic effects of garlic are thought to be due to its active ingredient allicin. This sulphur-containing compound gives garlic its distinctive pungent smell and taste. Garlic possesses antiviral, antibacterial, anti-fungal properties allowing it to stand against all infections. This work has focused on the evaluation and comparison of total content of polyphenols and antioxidant activity in five varieties of garlic - Mojmír, Záhorský, Lukan, Havran and Makoi. Samples of plant material were collected at the stage of full maturity in the area of Nitra. The total content of polyphenols was determined using the spectrophotometric method of Folin-Ciocalteu agents. Determined the content of total polyphenols in garlic were in the range 621.13 mg.kg-1 (Záhorský) to 763.28 mg.kg-1 (Havran). Total polyphenols content in garlic declined in the following order: Havran >Mojmír >Makoi >Lukan >Záhorský. Antioxidant activity was measured by the spectrophotometric method using a compound DPPH (2.2-diphenyl-1-picryhydrazyl). Statistically significant highest value of antioxidant was recorded in 20.22% (Mojmír) and the lowest value was in 13.61% (Záhorský). The values of antioxidant activity observed in the varieties of garlic may be arranged as follows: Mojmír >Havran >Lukan >Makoi >Záhorský. In all the analysed varieties of garlic was confirmed by the strong dependence of the antioxidant activity and the total content of polyphenols.
Act No. 220/2004 Coll. Of Laws of Slovak Republic. On the conservation and use of agricul- tural land, amending the Act No. 245/2003 Coll. on integrated pollution prevention and control, amending and supplementing of certain acts, as amended.
Batcioglu, K., Yilmaz, Z., Satilmis, B., Uyumlu, A. B., Erkal, H. S., Yucel, N., Gunals, S., Serin, M., Demirtas, H. 2012. Investigation of in vivo radioprotective and in vitro antioxidant and antimicrobial activity of garlic (Allium sativum). European Review for Medical and Pharmacological Sciences, vol. 16, suppl no. 3, p. 47-57. PMid:22957418
Brand-Williams, W., Cuvelier, M. E., Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft and Technologie, vol. 28, no. 1, p. 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5 DOI: https://doi.org/10.1016/S0023-6438(95)80008-5
Charles, D. J. 2013. Antioxidant Properties of Spices, Herbs and Other Sources. NY: Springer science. ISBN: 978-1-4614-4310-0. DOI: https://doi.org/10.1007/978-1-4614-4310-0
Chekki, R. Z., Snoussi, A., Hamrouni, I., Bouzouita, N. 2014. Chemical composition, antibacterial and antioxidant activities of Tunisian garlic (Allium sativum) essential oil and ethanol extract. Mediterranean Journal of Chemistry, vol. 3, no. 4, p. 947-956. https://doi.org/10.13171/mjc.3.4.2014.09.07.11 DOI: https://doi.org/10.13171/mjc.3.4.2014.09.07.11
Chen, S., Shen, X., Cheng, S., Li, P., Du, J., Chang, Y., Meng, H. 2013. Evaluation of Garlic Cultivars for Polyphenolic Content and Antioxidant Properties. Plos One, vol. 8, no. 11, p. e79730. https://doi.org/10.1371/journal.pone.0079730 DOI: https://doi.org/10.1371/journal.pone.0079730
Elhamidi, M. Y. 2010. The Contribution of Fruit and Vegetable Consumption to Human Health. In de la Rosa, L. A., Alvarez-Parrilla, E., González-Aguilar, G. A. Fruit and Vegetable Phytochemicals: Chemistry, Nutritional value and Stability. Wiley-Balcwell, p. 3-53. ISBN: 978-0-8138-0320-3.
Elhamidi, M., Elshami, S. M. 2015. Scavenging Activity of Differnet Garlic Extracts and Garlic Powder and their Antioxidant Effect on Heated Sunflower Oil. American Journal of Food Technology, vol. 10, no. 4, p. 135-146. https://doi.org/10.3923/ajft.2015.135.146 DOI: https://doi.org/10.3923/ajft.2015.135.146
Fritsch, M. R., Friesen, N. 2002. Evolution, domestication and taxonomy. Wallingford UK : CABI Publishing. ISBN: 0851995101. https://doi.org/10.1079/9780851995106.0005 DOI: https://doi.org/10.1079/9780851995106.0005
Fritsch, R. M., Blattner, F. R., Gurushidze, M. 2010. New classification of Allium L. subg. Melanocrommyum (Webb & Berthel) Rouy (Alliaceae) based on molecular and morphological characters. Phyton, vol. 49, no. 2, p. 145-220.
Hu, Ch. 2012. Factors affecting phytochemical composition and antioxidant activity of Ontario vegetable crops. Ontario Canada : Guelphl. 208 p. https://doi.org/10214/3592
Jastrzebski, Z., Leontowicz, H., Leontowicz, M., Namiesnik, J., Zachwieja, Z., Barton, H., Pawelzik, E., Arancibia-Avila, P., Toledo, F., Gorinstein, S. 2007. The bioactivity of processed garlic (Allium sativum L.) as shown in vitro and in vivo studies on rats. Food and Chemical Toxicology, vol. 45, no. 9, p. 1626-1633. https://doi.org/10.1016/j.fct.2007.02.028 DOI: https://doi.org/10.1016/j.fct.2007.02.028
Kim, J. W., Huh, J. E., Kyung, S. H., Kyung, K. H. 2004. Antimicrobial activity of alk(en)yl sulfides found in essential oils of garlic and onion. Food Science and Biotechnology, vol. 13, no. 2, p. 235-239.
Lachman, J., Proněk, D., Hejtmanková, A., Dudjak, J., Pivec, V., Faitová, K. 2003. Total polyphenol and main flavonoid antioxidant in different onion (Allium cepa L.) varieties. Horticultural Science, vol. 30, no. 4, p. 142-147. DOI: https://doi.org/10.17221/3876-HORTSCI
Lanzotti, V. 2006. The analysis of onion and garlic. Journal of Chromatography A, vol. 1112, no. 1-2, p. 3-22. https://doi.org/10.1016/j.chroma.2005.12.016 DOI: https://doi.org/10.1016/j.chroma.2005.12.016
Pérez-Gregorio, R. M., Garcia-Falcon, M. S., Simal-Gandara, J., Rodriguez, A. S., Almeida, D. P. F. 2009. Identification and quantification of flavonoids in traditional cultivars of red and white onions at harvest. Journal of Food Composition and Analysis, vol. 23, no. 6, p. 592-598. https://doi.org/10.1016/j.jfca.2009.08.013 DOI: https://doi.org/10.1016/j.jfca.2009.08.013
Ramkissoon, J. S., Mahomoodally, M. F., Ahmed, N., Subratty, A. H. 2012. Relationship between total phenolic content, antioxidant potencial, and antiglycation abilities of common culinary herbs and spices. Journal of Medical Food, vol. 15, no. 12, p. 1116-1123. https://doi.org/10.1089/JMF.2012.0113 DOI: https://doi.org/10.1089/jmf.2012.0113
Rizwani, G. H., Shareef, H. 2011. Genus Allium: The Potential Nutritive and Therapeutic Source. Journal of Pharmacy and Nutrition Sciences, vol. 1, no. 2, p. 158-165. https://doi.org/10.6000/1927-5951.2011.01.02.11 DOI: https://doi.org/10.6000/1927-5951.2011.01.02.11
Shalaby, E. A., Shanab, S. M. M. 2013. Antioxidant compounds, assays of determination ad mode of action. African Journal of Pharmacy and Pharmacology, vol. 7, no. 10, p. 528-539. https://doi.org/10.5897/AJPP2013.3474 DOI: https://doi.org/10.5897/AJPP2013.3474
Shebis, Y., Iluz, D., Kinel-Tahan, Y., Dubinski, Z., Yehoshua, Y. 2013. Natural Antioxidants: Function and Sources. Food and Nutrition Sciences, vol. 4, no. 6, p. 643-649. https://doi.org/10.4236/fns.2013.46083 DOI: https://doi.org/10.4236/fns.2013.46083
Srivastava, S., Pathak, P. H. 2012. Garlic (Allium sativum) extract supplementation alters the glycogen deposition in liver and protein metabolism in gonads of female albino rats. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 2, p. 126-129.
Tepe, B., Sokmen, M., Akpulat, H., Sokmen, A. 2005. In vitro antioxidant activities of the methanol extract of five species from Turkey. Food Chemistry, vol. 92, no. 1, p. 89-92. https://doi.org/10.1016/j.foodchem.2004.04.030 DOI: https://doi.org/10.1016/j.foodchem.2004.07.016
Trifunschi, S., Munteanu, M. F., Agotici, V., Pitea, S., Gligor, R. 2015. Determination of Flavonoid and Polyphenol Compounds in Viscum Album and Allium sativum Extract. International Current Pharmaceutical Journal, vol. 4, no. 5, p. 382-385. https://doi.org/10.3329/icpj.v4i5.22861 DOI: https://doi.org/10.3329/icpj.v4i5.22861
Vlase, L., Parvu, M., Parvu, E. A., Toiu, A. 2013. Chemical Constituents of Three Allium Species from Romania. Molecules, vol. 18, no. 1, p. 114-127. https://doi.org/10.3390/molecules18010114 DOI: https://doi.org/10.3390/molecules18010114
Wangcharoen, W., Morasuk, W. 2007. Antioxidant capacity and phenolic content of some Thai culinary plants. Maejo International Journal of Science and Technology, vol. 1, p. 100-106.
Wangcharoen, W., Morasuk, W. 2009. Effect of heat treatment on the antioxidant capacity of garlic. Maejo International Journal of Science and Technology, vol. 3, no. 1, p. 60-70.
Yang, J., Meyers, K. J., Van Der Heide, J., Liu, R. H. 2004. Varietal differences in phenolic content and antioxidant and antiproliferative activities of onions. Journal of Agricultural and Food Chemistry, vol. 52, no. 22, p. 6787-6793. https://doi.org/10.1021/JF0307144 DOI: https://doi.org/10.1021/jf0307144
Young, I. S., Woodside, J. V. 2001. Antioxidants in health and disease. Journal of Clinical Pathology, vol. 54, no. 3, p. 176-186. https://doi.org/ 10.1136/jcp.54.3.176 DOI: https://doi.org/10.1136/jcp.54.3.176
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