The improvement of insulin resistance and the antioxidant capacity in type 2 diabetes mellitus rats with whiteleg shrimp shell powder (Litopenaeus vannamei)


  • Risya Ahriyasna Universitas Diponegoro and Universitas Perintis Indonesia, Faculty of Medicine and Health Sciences, Department of Nutritional Science, Adinegoro Lubuk Buaya, 25586, Padang, Indonesia, Tel.: +62751481992
  • Tri Winarni Agustini Universitas Diponegoro, Faculty of Fisheries and Marine Sciences, Department of Fish Product Technology, Prof. H. Soedarto, 50275 , Semarang, Indonesia, Tel.: +62247474698
  • Kis Djamiatun Universitas Diponegoro, Faculty of Medicine, Department of Parasitology, Prof. Soedarto, 50275, Semarang, Indonesia, Tel.: +622476928010
  • Def Primal Universitas Perintis Indonesia, Faculty of Health Sciences, Department of Anatomy and Physiology, Adinegoro Lubuk Buaya, 25586, Padang, Indonesia, Tel.: +6281348305867



astaxanthin, HOMA-IR, antioxidant, T2DM, whiteleg shrimp shell powder


As estimated having an increased incidence of about 50% until 2040, the diabetic condition could be augmented primarily from astaxanthin contained in carotenoids. This research examines and compares the influence of WSSP and AST complement on Homeostatic Model Assessment-Insulin Resistance (HOMA-IR) level and Total Antioxidant Capacity (TAC) induced high-fat diet streptozotocin (HFD-STZ) in T2DM rats. WSSP 0.89gr/200gr/d (X1) and 1.77gr/200gr/d (X2) groups; and AST supplement 0.09mg/200gr/d (X3) groups were compared with both of positive (K1) and negative (K2) groups. The treatments were administered orally for 21 days to 25 Wistar rats which each group consisted of 5 rats. HOMA-IR and TAC levels were measured by ELISA and ABTs method respectively. TAC levels significantly increased in treatment groups than K1 group (p = 0.008). The increase in the TAC level of the X2 group was greater than the X1 group (p = 0.017). HOMA IR levels were significantly lower in treatment groups than K1 group (p = 0.009). X2 group had a greater decrease in HOMA IR levels compared to X1 (p = 0.016). In consequence, the research findings show a utilitarian effect of WSSP in increasing TAC and decreasing the HOMA-IR index.


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Affandi, M. M., Julianto, T., Majeed, A. 2012. Enhanced oral bioavailability of astaxanthin with droplet size reduction. Food Science and Technology Research, vol. 18, p. 549-554. DOI:

Al-Bulish, M. S. M., Xue, C., Waly, M. I., Xu, J., Wang, Y., Tang, Q.J. 2017. The Defensive Role of Antioxidants Astaxanthin against Oxidative Damage in Diabetic Rats Injected with Streptozotocin. Journal of Food and Nutrition Research, vol. 5, no. 3, p. 191-196.

Al-Goblan, A. S., Al-Alfi, M. A., Khan, M. Z. 2014. Mechanism linking diabetes mellitus and obesity. Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy, vol. 7, p. 587-591. DOI:

Ambati, R. R., Phang, S. M., Ravi, S., Aswathanarayana, R. G. 2014. Astaxanthin: sources, extraction, stability, biological activities and its commercial applications--a review. Marine Drugs, vol. 12, no. 1, p. 128-152. DOI:

Antunes, L. C., Elkfury, J. L., Jornada, M. N., Foletto, K. C., Bertoluci, M. C. 2016. Validation of HOMA-IR in a model of insulin-resistance induced by a high-fat diet in Wistar rats. Archives of Endocrinology and Metabolism, vol. 60, no. 2, p. 138-142. DOI:

Aouacheri, O., Saka, S., Krim, M., Messaadia, A., Maidi, I. 2015. The investigation of the oxidative stress-related parameters in type 2 diabetes mellitus. Canadian Journal of Diabetes, vol. 39, no. 1, p. 44-49. DOI:

Asmat, U., Abad, K., Ismail, K. 2016. Diabetes mellitus and oxidative stress-A concise review. Saudi Pharmaceutical Journal : SPJ : the official publication of the Saudi Pharmaceutical Society, vol. 24, no. 5, p. 547-553. DOI:

Ayudiarti, D. L. 2011. Ekstraksi Karotenoid Dari Kepala Udang Secara Enzimatis Dan Karakterisasi Profil Karotenoid Sebagai Antioksidan (Enzymatic Extraction of Carotenoids from Shrimp Heads and Characterization of Carotenoid Profiles as Antioxidants). Bogor: Teknologi Hasil Perairan, Universitas Pertanian Bogor; (in Indonesia). Available at:

Benarroch, M. M., Hugot, C., Rhazi, L., Niamba, C. N., Depeint, F. 2016. The Bioavailability of Astaxanthin Is Dependent on Both the Source and the Isomeric Variants of the Molecule. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technolog., vol. 73, no. 2, p. 61-69. DOI:

Cahú, T. B., Santos, S. D., Mendes, A., Córdula, C. R., Chavante, S. F., Carvalho, L. B. 2012. Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste. Elsevier: Process Biochemistry, vol. 47, p. 570-577. DOI:

Chan, K. C., Pen, P. J., Yin, M. C. 2012. Anticoagulatory and antiinflammatory effects of astaxanthin in diabetic rats. Journal of food science, vol. 77, no. 2, p. H76-H80. DOI:

Choi, H. D., Kim, J. H., Chang, M. J., Kyu-Youn, Y., Shin, W. G. 2011. Effects of astaxanthin on oxidative stress in overweight and obese adults. Phytotherapy Research, vol. 25, no. 12, p. 1813-1818. DOI:

Corrêa, N. C. F, Macedo, C. D. S., Moraes, J. D. F. C., Machadoa, N. T., Franc, L. F. D. 2012. Characteristics of the extract of Litopenaeus vannamei shrimp obtained from the cephalothorax using pressurized CO2. The Journal of Supercritical Fluids, vol. 66, p. 176-180. DOI:

Ghasemi, A., Khalifi, S., Jedi, S. 2014. Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). Acta physiologica Hungarica, vol. 101, no. 4, 408-420. DOI:

Hodgkin, M. N., Hills, C. E., & Squires, P. E. 2008. The calcium-sensing receptor and insulin secretion: a role outside systemic control 15 years on. The Journal of endocrinology, vol. 199, no. 1, p. 1-4. DOI:

Hue, H. T., Chien, N. Q., Anh, L. L., Thuy, T. T. T. 2008. Determination of Astaxanthin and other Carotenoids in Vietnamese Crustaceans by HPLC.: Tap Chi Khoa Hoc Cong Nghe: Journal of Science and Technology, vol. 46, no. 3, p. 47-58.

International Diabetes Federation. 2017. IDF Western Pacific Member (Indonesia). Available at:

Khan, M., Nowsad, A. 2013. Development of protein enriched shrimp crackers from shrimp shell wastes. Journal of the Bangladesh Agricultural University, vol. 10, no. 2, p. 367-374. DOI:

Kupsal, K., Mudigonda, S., Gundapaneni, K. K, Tupurani, M. A., Galimudi, R. K., Nyayapathi, V. B. K. S., et al. 2015. Glucotoxicity and lipotoxicity induced beta-cell apoptosis in type 2 diabetes mellitus. International Journal of Analytical Bio-Science, vol. 3, no. 4, p. 84-89.

Li, X., Qi, Z., Zhao, L., Yu, Z. 2016. Astaxanthin reduces type 2 diabetic associated cognitive decline in rats via activation of PI3K/Akt and attenuation of oxidative stress. Molecular Medicine Reports, vol. 13, p. 973-979. DOI:

Liu, S. H., Chang, Y. H., Chiang, M. T. 2010. Chitosan reduces gluconeogenesis and increases glucose uptake in skeletal muscle in streptozotocin-induced diabetic rats. Journal of Agricultural and Food Chemistry, vol. 58, no. 9, p. 5795-5800. DOI:

Mitri, J., Dawson-Hughes, B., Hu, F. B., Pittas, A. G. 2011. Effects of vitamin D and calcium supplementation on pancreatic β cell function, insulin sensitivity, and glycemia in adults at high risk of diabetes: the Calcium and Vitamin D for Diabetes Mellitus (CaDDM) randomized controlled trial. The American Journal of Clinical Nutrition, vol. 94, no. 2, p. 486-494. DOI:

Miyata, T., Notoya, K., Yoshida, K., Horie, K., Maeda, K., Kurokawa, K., Taketomi, S. 1997. Advanced glycation end products enhance osteoclast-induced bone resorption in cultured mouse unfractionated bone cells and in rats implanted subcutaneously with devitalized bone particles. Journal of the American Society of Nephrology : JASN, vol. 8, no. 2, p. 260-270. DOI:

Nair, S., Gagnon, J., Pelletier, C., Tchoukanova, N., Zhang, J., Ewart, H. S., Ewart, K. V., Jiao, G., Wang, Y. 2017. Shrimp oil extracted from the shrimp processing waste reduces the development of insulin resistance and metabolic phenotypes in diet-induced obese rats. Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquee, Nutrition et Metabolisme, vol. 42, no. 8, p. 841-849. DOI:

Nikooyeh, B., Neyestani, T. R., Farvid, M., Alavi-Majd, H., Houshiarrad, A., Kalayi, A., Shariatzadeh, N., Gharavi, A., Heravifard, S., Tayebinejad, N., Salekzamani, S., Zahedirad, M. 2011. Daily consumption of vitamin D- or vitamin D + calcium-fortified yogurt drink improved glycemic control in patients with type 2 diabetes: a randomized clinical trial. The American Journal of Clinical Nutrition, vol. 93, no. 4, p. 764-771. DOI:

Nolan, C. J., Damm, P., Prentki, M. 2011. Type 2 diabetes across generations: from pathophysiology to prevention and management. Lancet (London, England), vol. 378, no 9786, p. 169-181. DOI:

Primal, D., Putri, T. and Meiriza, W. 2021. Asupan Karbohidrat Harian terhadap Risiko Kejadian Diabetes Melitus Gestasi (GDM) melalui Glyco-Hemoglobin Ibu Hamil (Daily Carbohydrate Intake on the Risk of Gestational Diabetes Mellitus (GDM) through Glyco-Hemoglobin in Pregnant Women). Jurnal Keperawatan Silampari, vol. 4, no. 2, p. 510-518. (in Indonesia) DOI:

Samuel, V. T., Shulman, G. I. 2012. Mechanisms for insulin resistance: common threads and missing links. Cell, vol. 148, no. 5, p. 852-871. DOI:

Sathananthan, M., Shah, M., Edens, K. L., Grothe, K. B., Piccinini, F., Farrugia, L. P. 2015. Six and 12 Weeks of Caloric Restriction Increases b Cell Function and Lowers Fasting and Postprandial Glucose Concentrations in People with Type 2 Diabetes. The Journal of Nutrition: Nutrient Physiology, Metabolism, and Nutrient-Nutrient Interactions, vol. 145, no. 9, p. 2046-2051. DOI:

Savu, O., Ionescu-Tirgoviste, C., Atanasiu, V., Gaman, L., Papacocea, R., Stoian, I. 2012. Increase in total antioxidant capacity of plasma despite high levels of oxidative stress in uncomplicated type 2 diabetes mellitus. The Journal of International Medical Research, vol. 40, no. 2, p. 709-716. DOI:

Sears, B., Perry, M. 2015. The role of fatty acids in insulin resistance. Lipids Health Dis., vol. 14, p. 121. DOI:

Shiloah, E., Witz, S., Abramovitch, Y., Cohen, O., Buchs, A., Ramot, Y., et al. 2003. Effect of Acute Psychotic Stress in Nondiabetic Subjects on -Cell Function and Insulin Sensitivity. Diabetes Care, vol. 26, no. 5, p. 1462-1467. DOI:

Shofian, N. M., Hamid, A. A., Osman, A., Saari, N., Anwar, F., Dek, M. S., Hairuddin, M. R. 2011. Effect of freeze-drying on the antioxidant compounds and antioxidant activity of selected tropical fruits. International Journal of Molecular Sciences, vol. 12, no. 7, p. 4678-4692. DOI:

Sila, A., Kamoun, Z., Ghlissi, Z., Makni, M., Nasri, M., Sahnoun, Z., Nedjar-Arroume, N., Bougatef, A. 2015. Ability of natural astaxanthin from shrimp by-products to attenuate liver oxidative stress in diabetic rats. Pharmacological reports : PR, vol. 67, no. 2, p. 310-316. DOI:

Suptijah, P., Jacoeb, A. M., Deviyanti, N. 2012. Karakterisasi dan bioavailabilitas nanokalsium cangkang udang vannamei (Litopenaeus vannamei). (Characterization and bioavailability of calsium nanoparticless vannamei shell (Litopenaeus vannamei)). Jurnal Akuatika., vol. 3, no. 1, p. 63-73 (in Indonesia).

Yang, S., Zhou, Q., Yang, L., Xue, Y., Xu, J., Xue, C. 2015. Effect of thermal processing on astaxanthin and astaxanthin esters in pacific white shrimp Litopenaeus vannamei. Journal of Oleo Science, vol. 64, no. 3, p. 243-253. DOI:

Ye J. 2013. Mechanisms of insulin resistance in obesity. Frontiers of Medicine, vol. 7, no. 1, p. 14-24. DOI:



How to Cite

Ahriyasna, R., Agustini, T. W., Djamiatun, K., & Primal, D. (2021). The improvement of insulin resistance and the antioxidant capacity in type 2 diabetes mellitus rats with whiteleg shrimp shell powder (Litopenaeus vannamei). Potravinarstvo Slovak Journal of Food Sciences, 15, 703–711.

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