Influence of eight chosen essential oils in the vapor phase on the growth of Rhizopus stolonifer and Rhizopus lyococcus

Authors

  • Dana Tančinová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, +421 37641 4433 https://orcid.org/0000-0001-6790-8169
  • Miroslava Hlebová University of SS. Cyril and Methodius, Faculty of Natural Sciences, Department of Biology, Nám. J. Herdu 2, SK–91701 Trnava, Slovakia, +421 33 5565 337 https://orcid.org/0000-0002-1720-9981
  • Denisa Folitinova Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
  • Zuzana Mašková Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, +421 37641 4432 https://orcid.org/0000-0003-3413-9337
  • Zuzana Barboráková Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, +421 37 6414494

DOI:

https://doi.org/10.5219/1586

Keywords:

essential oils, Rhizopus stolonifer, Rhizopus lyococcus, antifungal activity, vapour phase

Abstract

This study aimed to evaluate the fungicidal effect of eight essential oils against five strains of the genus Rhizopus. Strains were obtained from various moldy foods,  namely Rhizopus stolonifer KMi 383 from chestnut, R. stolonifer KMi 510 from strawberry, R. stolonifer KMi 511 from nectarine, R. stolonifer KMi 524 from cherry tomatoes, and R. lyococcus KMi 512 from blackberry. The essential oils (EO) used in this study were jasmine EO (extract from Jasminum officinale L.), bergamot EO (Mentha aquatica L. var. citrata (Her.) Fresen), bitter orange EO (from Citrus aurantium L.), grapefruit EO (Citrus paradisi Macfady), sweet flag EO (East Asian Calamus, from Acorus calamus L. var. angustatus Bes), star anise EO (from Illicium verum J.D.Hook), geranium EO (from Pelargonium graveolens), and lemongrass EO (from Cymbopogon citratus DC). The semi-quantitative composition of the essential oil samples was determined by gas chromatography coupled with mass spectrometry (GC-MS). The antifungal activity of essential oils against the strains of R. stolonifer and R. lyococcus was determined during 7 days, using the micro-atmosphere method (0.625 μL.mL-1 of air). Two essential oils, geranium and lemongrass, completely inhibited the growth of all isolates. Bitter orange essential oil inhibited the growth of all isolates of Rhizopus stolonifer, but isolate of Rhizopus lyococcus began to grow after four days of cultivation. In conclusion, certain essential oils are highly effective in the vapor phase. These could be used in further tests of their antifungal activity and could be used in the control of Rhizopus spp. or other fungal pathogens.

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References

Abdulazeez, M. A., Abdullahi, A. S., James, B. D. 2016. Lemongrass (Cymbopogon spp.) oils. In Preedy, V. R. Essential Oils in Food Preservation, Flavor and Safety. USA : Elsevier, p. 509-516. ISBN 978-0-12-416641-7. https://doi.org/10.1016/b978-0-12-416641-7.00058-4

Adams, B., Gavin, C. L., McGowen, M. M. Waddell, W. J., Cohen, S. M., Feron, V. J., Marnett, L. J., Munro, I. C. Portoghese, P. S., Rietjens, I. M. C. M., Smith, R. L. 2011. The FEMA GRAS assessment of aliphatic and aromatic terpene hydrocarbons used as flavor ingredients. Food and Chemical Toxicology, vol. 49, no. 10, p. 2471-2494. https://doi.org/10.1016/j.fct.2011.06.011

Ahmed, N., Hanani, Y. A., Ansari, S. Y., Anwar, S. 2016. Jasmine (Jasminum sambac L., Oleaceae) oils. In Preedy, V. R. Essential Oils in Food Preservation, Flavor and Safety. USA : Elsevier, p. 487-494, ISBN 978-0-12-416641-7. https://doi.org/10.1016/b978-0-12-416641-7.00055-9

Ali, A., Noh, N. M., Mustafa, M. A. 2015. Antimicrobial activity of chitosan enriched with lemongrass oil against anthracnose of bell pepper. Food Packaging and Shelf Life, vol. 3, p. 56-61. https://doi.org/10.1016/j.fpsl.2014.10.003

Alizadeh-Salteh, S., Arzani, K., Omidbeigi, R., Safaie, N., Mohammadi, P. 2013. Savory essential oil effect on postharvest control of Rhizopus rot on packaged peaches in polyethylene films. Acta Horticulturae, vol. 1012, p. 759-762. https://doi.org/10.17660/actahortic.2013.1012.102

Alizadeh-Salteh, S., Arzani, K., Omidbeili, R., Safaie, N. 2010. Essential oils inhibit mycelial growth of Rhizopus stolonifer. European Journal of Horticurtural Science, vol. 75, no. 6, p. 278-282.

Anwar, S., Ahmed, N., Speciale, A., Cimino, F., Saija, A. 2016. Bitter orange (Citrus aurantium L.) oils. In Preedy, V. R. Essential Oils in Food Preservation, Flavor and Safety, USA : Elsevier, p. 259-268. ISBN 978-0-12-416641-7. https://doi.org/10.1016/b978-0-12-416641-7.00029-8

Avila-Sosa, R., Navarro-Cruz, A. R., Sosa-Morales, M. E., López-Malo, A., Palou, E. 2016. Bergamot (Citrus bergamia) oils. In Preedy, V. R. Essential Oils in Food Preservation, Flavor and Safety, USA : Elsevier, p. 247-252. ISBN 978-0-12-416641-7. https://doi.org/10.1016/b978-0-12-416641-7.00027-4

Bautista-Baños, S., Bosquez-Molina, E., Barrera-Necha, L. L. 2014. Rhizopus stolonifer (soft rot). In Bautista-Baños, S. Postharvest Decay. Amsterdam, Netherland : Elsevier, p. 1-44. ISBN 978-0-12-411552-1. https://doi.org/10.1016/b978-0-12-411552-1.00001-6

Ben Farhat, M., Jordán, M. J., Chaouch-Hamada, R., Landoulsi, A., Sotomayor, J. A. 2016. Phenophase effects on sage (Salvia officinalis L.) yield and composition of essential oil. Journal of Applied Research on Medicinal and Aromatic Plants, vol. 3, no. 3, p. 87-93. https://doi.org/10.1016/j.jarmap.2016.02.001

Bhaskara Reddy, M. V., Angers, P., Gosselin, A., Arul, J. 1998. Characterization and use of essential oil from Thymus vulgaris against Botrytis cinerea and Rhizopus stolonifer in strawberry fruits. Phytochemistry, vol. 47, no. 8, p. 1515-1520. https://doi.org/10.1016/s0031-9422(97)00795-4

Bhattacharya, S. 2016. Cultivation of essential oils. In Preedy, V. R. Essential Oils in Food Preservation, Flavor and Safety, USA : Elsevier, p. 19-29. ISBN 978-0-12-416641-7. https://doi.org/10.1016/B978-0-12-416641-7.00003-1

Bosquez-Molina, E., Jesús, E. R., Bautista-Baños, S., Verde-Calvo, J. R., Morales-López, J. 2010. Inhibitory effect of essential oils against Colletotrichum gloeosporioides and Rhizopus stolonifer in stored papaya fruit and their possible application in coatings. Postharvest Biology and Technology, vol. 57, no. 2, p. 132-137. https://doi.org/10.1016/j.postharvbio.2010.03.008

Bouzenna, H., Krichen, L. 2013. Pelargonium graveolens L’Her. and Artemisia arborescens L. essential oils: Chemical composition, antifungal activity against Rhizoctonia solani and insecticidal activity against Rhysopertha dominica. Natural Product Research, vol. 27, no. 9, p. 841-846. https://doi.org/10.1080/14786419.2012.711325

Božik, M., Císarová, M., Tančinová,D., Kouřimská, L., Hleba, L., Klouček, P. 2017. Selected essential oil vapours inhibit growth of Aspergillus spp. in oats with improved consumer acceptability. Industrial Crops and Products, vol. 98, p. 146-152. https://doi.org/10.1016/j.indcrop.2016.11.044

Cakir, A., Kordali, S., Kilic, H., Kaya, E. 2005. Antifungal properties of essential oil and crude extracts of Hypericum linarioides Bosse. Biochemical Systematics and Ecology, vol. 33, no. 3, p. 245-256. https://doi.org/10.1016/j.bse.2004.08.006

Císarová, M., Hleba, L., Medo, J., Tančinová, D., Mašková, Z., Čuboň, J., Kováčik, A., Foltinová, D., Božik, M., Klouček, P. 2020. The in vitro and in situ effect of selected essential oils in vapour phase against bread spoilage toxicogenic aspergilli. Food Control, vol. 110, p. 107007. https://doi.org/10.1016/j.foodcont.2019.107007

Combrinck, S., Regnier, T., Kamatou, G. P. P. 2011. In vitro activity of eighteen essential oils and some major components against common postharvest fungal pathogens of fruit. Industrial Crops and Products, vol. 33, no. 2, p. 344-349. https://doi.org/10.1016/j.indcrop.2010.11.011

da Rocha Neto, A. C., Navarro, B. B., Canton, L., Maraschin, M., di Piero, R. M. 2019. Antifungal activity of palmarosa (Cymbopogon martinii), tea tree (Melaleuca alternifolia) and star anise (Illicium verum) essential oils against Penicillium expansum and their mechanisms of action. LWT - Food Science and Technology, vol. 105, p. 385-392. https://doi.org/10.1016/j.lwt.2019.02.060

Dušková, E., Dušek, K., Indrák, P., Smékalová, K. 2016. Postharvest changes in essential oil content and quality of lavender flowers. Industrial Crops and Products, vol. 79, p. 225-231. https://doi.org/10.1016/j.indcrop.2015.11.007

Guynot, M. E., Ramos, A. J., Seto, L., Purroy, P., Sanchis, V., Marin, S. 2003. Antifungal activity of volatile compounds generated by essential oils against fungi commonly causing deterioration of bakery products. Journal of Applied Microbiology, vol. 94, no. 5, p. 893-899. https://doi.org/10.1046/j.1365-2672.2003.01927.x

Hadian, J., Ghasemnezhad, M., Ranjbar, H., Frazane, M., Ghorbanpour, M. 2008. Antifungal Potency of Some Essential Oils in Control of Postharvest Decay of Strawberry Caused by Botrytis cinerea, Rhizopus stolonifer and Aspergillus niger. Journal of Essential Oil Bearing Plants, vol. 11, no. 5, p. 553-562. https://doi.org/10.1080/0972060X.2008.10643666

Ju, J., Chen, X., Xie, Y., Yu, H., Guo, Y., Cheng, Y., Yao, W. 2019. Application of essential oil as a sustained release preparation in food packaging. Trends in Food Science & Technology, vol. 92, p. 22-32. https://doi.org/10.1016/j.tifs.2019.08.005li

Kassemeyer, H. H., Berkelmann-Löhnertz, B. 2009. Fungi of grapes. In König, H., Unden, G., Fröhlich, J. Biology of Microorganisms on Grapes, in Must and in Wine. Berlin, Heidelberg, Germany : Springer-Verlag, p. 61-87. ISBN 978-3-540-85463-0.

Kordali, S., Cakir, A., Ozer, H., Cakmakci, R., Kesdek, M., Mete, E. 2008. Antifungal, phytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Bioresource Technology, vol. 99, no. 18, p. 8788-8795. https://doi.org/10.1016/j.biortech.2008.04.048

Kwon, J. H., Kang, S. W., Kim, J. S., Park, C. S. 2001. Rhizopus soft rot on cherry tomato caused by Rhizopus stolonifer in Korea. Mycobiology, vol. 29, no. 3, p. 176-178. https://doi.org/10.1080/12298093.2001.12015783

Leimann, F. V., Gonçalves, O. H., Machado, R. A. F., Bolzan, A. 2009. Antimicrobial activity of microencapsulated lemongrass essential oil and the effect of experimental parameters on microcapsules size and morphology. Materials Science and Engineering: C, vol. 29, no. 2, p. 430-436. https://doi.org/10.1016/j.msec.2008.08.025

López, P., Sánchez, C., Batlle, R., Nerín, C. 2007. Vapor-phase activities of cinnamon, thyme, and oregano essential oils and key constituents against foodborne microorganisms. Journal of Agricultural and Food Chemistry, vol. 55, no. 11, p. 4348-4356. https://doi.org/10.1021/jf063295u

Mari, M., Bautista-Baños, S., Sivakumar, D. 2016. Decay control in the postharvest system: Role of microbial and plant volatile organic compounds. Postharvest Biology and Technology, vol. 122, p. 70-81. https://doi.org/10.1016/j.postharvbio.2016.04.014

Méndez-Tovar, I., Novak, J., Sponza, S., Herrero, B., Asensio-S-Manzanera, M. C. 2016. Variability in essential oil composition of wild populations of Labiatae species collected in Spain. Industrial Crops and Products, vol. 79, p. 18-28. https://doi.org/10.1016/j.indcrop.2015.10.009

Miao, J.-K., Shi, R.-H., Li, C., Li, X.-W., Chen, Q.-X. 2016. Sweet flag (Acorus calamus) Oils. In Preedy, V. R. Essential Oils in Food Preservation, Flavor and Safety, USA : Elsevier, p. 775-782. ISBN 978-0-12-416641-7. https://doi.org/10.1016/b978-0-12-416641-7.00088-2

Moore-Neibel, K., Gerber, C., Patel, J., Friedman, M., Ravishankar, S. 2011. Antimicrobial activity of lemongrass oil against Salmonella enterica on organic leafy greens. Journal of Applied Microbiology, vol. 112, no. 3, p. 485-492. https://doi.org/10.1111/j.1365-2672.2011.05222.x

Naeini, A. R., Nazeri, M., Shokri, H. 2011. Antifungal activity of Zataria multiflora, Pelargonium graveolens and Cuminum cyminum essential oils towards three species of Malassezia isolated from patients with pityriasis versicolor. Journal de Mycologie Médicale, vol. 21, no. 2, p. 87-91. https://doi.org/10.1016/j.mycmed.2011.01.004

Nedorostova, L., Kloucek, P., Kokoska, L., Stolcova, M., Pulkrabek, J. 2009. Antimicrobial properties of selected essential oils in vapour phase against foodborne bacteria. Food Control, vol. 20, no. 2, p. 157-160. https://doi.org/10.1016/j.foodcont.2008.03.007

Ng, T. B., El-Din Ahmed Bekhit, A., Fang, E. F., Li, X., Lu, Q., Guo, H., Wong, J. H. 2016. Grapefruit (Citrus paradisii) oils. In Preedy, V. R. Essential Oils in Food Preservation, Flavor and Safety, USA : Elsevier, p. 463-470. ISBN 978-0-12-416641-7. https://doi.org/10.1016/b978-0-12-416641-7.00052-3

Pitt, J. I., Hocking, A. D. 2009. Fungi and food spoilage. 3rd ed. London, New York : Springer Science & Business Media, LLC, 519 p. ISBN 978-0-387-92206-5.

SAS/9.3. 2010. Copyright (c) 2002-2010 by SAS Institute Inc., Cary, NC, USA.

Stević, T., Berić, T., Šavikin, K., Soković, M., Gođevac, D., Dimkić, I., Stanković, S. 2014. Antifungal activity of selected essential oils against fungi isolated from medicinal plant. Industrial Crops and Products, vol. 55, p. 116-122. https://doi.org/10.1016/j.indcrop.2014.02.011

Tripathi, P., Shukla, A. K. 2009. Application of essential oils for postharvest control of stem end rot of mango fruits during storage. International Journal of Postharvest Technology and Innovation, vol. 1, no. 4, p. 405-415. https://doi.org/10.1504/ijpti.2009.030689

Tyagi, A. K., Malik, A. 2011. Antimicrobial potential and chemical composition of Eucalyptus globulus oil in liquid and vapour phase against food spoilage microorganisms. Food Chemistry, vol. 126, no. 1, p. 228-235. https://doi.org/10.1016/j.foodchem.2010.11.002

Verma, R. S., Chandra Padalia, R., Chauhan, A. 2016. Rose-Scented Geranium (Pelargonium sp.) Oils. In Preedy, V. R. Essential Oils in Food Preservation, Flavor and Safety, USA : Elsevier, p. 697-704. ISBN 978-0-12-416641-7. https://doi.org/10.1016/b978-0-12-416641-7.00079-1

Viuda-Martos, M., Ruiz-Navajas, Y., Fernández-López, J., Pérez-Álvarez, J. 2008. Antifungal activity of lemon (Citrus lemon L.), mandarin (Citrus reticulata L.), grapefruit (Citrus paradisi L.) and orange (Citrus sinensis L.) essential oils. Food Control, vol. 19, no. 12, p. 1130-1138. https://doi.org/10.1016/j.foodcont.2007.12.003

Published

2021-04-28

How to Cite

Tančinová, D., Hlebová, M., Folitinova, D., Mašková, Z., & Barboráková, Z. (2021). Influence of eight chosen essential oils in the vapor phase on the growth of Rhizopus stolonifer and Rhizopus lyococcus. Potravinarstvo Slovak Journal of Food Sciences, 15, 378–386. https://doi.org/10.5219/1586

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