Alternaria SPP. in food commodities of Slovak origin: occurrence and mycotoxin production abilities

Authors

  • Zuzana Mašková Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414432
  • Dana Tančinová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414433
  • Miriam Ballová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia

DOI:

https://doi.org/10.5219/1102

Keywords:

cereals, grapes, juices, mycotoxin, Alternaria spp.

Abstract

Various food commodities of Slovak origin were analysed for the occurrence of Alternaria species-groups. Totally we analysed 14 samples of grapes, 3 samples of barley, 2 samples of wheat, 17 samples of fruit, vegetable and fruit-vegetable juices, 6 samples of red kuri squash with macroscopically visible infection. Mycological analyses were performed by using plate dilution method, method of direct placing of berries or grains on the plates with dichloran, rose bengal and chloramphenicol agar or by direct inoculation by mycological needle to the identification medium (potato-carrot agar). In all grape, barley, wheat and squash samples the presence of representatives of this genus was detected (100% isolation frequency). In juices, 41% of the samples were positive for their occurrence. The highest relative density of Alternaria isolates was found in grape samples (87%). All detected strains were segregated into four morphological species-groups: A. alternata, A. arborescens, A. infectoria and A. tenuissima. The most dominant species-group in grapes was A. arborescens, in barley and wheat A. tenuissima, followed by A. alternata, in juices only A. alternata and A. arborescens species-groups were detected and isolates of squashes were not classified to the species-groups. Randomly selected 67 isolates were analysed for the ability to produce mycotoxins alternariol (AOH), alternariol monomethylether (AME) and altenuene (ALT) by means of thin-layer chromatography. Of all tested isolates, AOH production was most frequently reported (70% of tested isolates). AME was produced by 60% and ALT by 49% of tested isolates. The largest share of the productive strains originated from the squashes, where all tested isolates produced ALT and AOH, followed by isolates of juices. From the viewpoint of individual species-groups, A. arborescens isolates and Alternaria spp. appeared to be the most productive in all mycotoxins tested.

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References

Andersen, B., Thrane, U. 1996. Secondary metabolites produced by Altemaria infectoria and their use as chemotaxonomic markers. Mycotoxin Research, vol. 12, no.2, p. 54-60. https://doi.org/10.1007/bf03192262 DOI: https://doi.org/10.1007/BF03192262

Andersen, B., Kroger, E., Roberts, R. G. 2001. Chemical and morphological segregation of Alternaria alternata, A. gaisen and A. longipes. Mycological Research, vol. 105, no. 3, p. 291-299. https://doi.org/10.1017/s0953756201003446 DOI: https://doi.org/10.1017/S0953756201003446

Andersen, B., Kroger, E., Roberts, R. G. 2002. Chemical and morphological segregation of Alternaria arborescens, A. infectoria and A. tenuissima species-groups. Mycological Research, vol. 106, no. 2, p. 170-182. https://doi.org/10.1017/s0953756201005263 DOI: https://doi.org/10.1017/S0953756201005263

Andersen, B., Sørensen, J. L., Nielsen, K. F., van den Ende, B. G., de Hoog, S. 2009. A polyphasic approach to the taxonomy of the Alternaria infectoria species–group. Fungal Genetics and Biology, vol. 46, no. 9, p. 642-656. https://doi.org/10.1016/j.fgb.2009.05.005 DOI: https://doi.org/10.1016/j.fgb.2009.05.005

Andersen, B., Nielsen, K. F., Fernández Pinto, V., Patriarca, A. 2015. Characterization of Alternaria strains from Argentinean blueberry, tomato, walnut and wheat. International Journal of Food Microbiology, vol. 196, p. 1-10. https://doi.org/10.1016/j.ijfoodmicro.2014.11.029 DOI: https://doi.org/10.1016/j.ijfoodmicro.2014.11.029

Asam, S., Konitzer, K., Schieberle, P., Rychlik, M. 2009. Stable Isotope Dilution Assays of Alternariol and Alternariol Monomethyl Ether in Beverages. Journal of Agricultural and Food Chemistry, vol. 57, no. 12, p. 5152-5160. https://doi.org/10.1021/jf900450w DOI: https://doi.org/10.1021/jf900450w

Da Cruz Cabral, L., Fernández Pinto, V., Patriarca, A. 2016. Control of infection of tomato fruits by Alternaria and mycotoxin production using plant extracts. European Journal of Plant Pathology, vol. 145, no. 2, p. 363-373. https://doi.org/10.1007/s10658-015-0850-1 DOI: https://doi.org/10.1007/s10658-015-0850-1

Dugan, F. M., Peever, T. L. 2002. Morphological and cultural differentiation of described species of Alternaria from Poaceae. Mycotaxon, vol. 83, p. 229-264.

EFSA. 2011. Scientific Opinion on the risks for animal and public health related to the presence of Alternaria toxins in feed and food. EFSA Journal, vol. 9, no. 10, p. 2407. https://doi.org/10.2903/j.efsa.2011.2407 DOI: https://doi.org/10.2903/j.efsa.2011.2407

Escrivá, L., Oueslati, S., Font, G., Manyes, L. 2017. Alternaria Mycotoxins in Food and Feed: An Overview. Journal of Food Quality, vol. 2017, p. 1-20. https://doi.org/10.1155/2017/1569748 DOI: https://doi.org/10.1155/2017/1569748

Gonzáles, H. H. L., Pacin, A., Resnik, S. L., Martinez, E. J. 1996. Deoxynivalenol and contaminant mycoflofa in freshly harvested Argentinean wheat in 1993. Mycopathologia, vol. 135, no. 2, p. 129-134. https://doi.org/10.1007/bf00436463 DOI: https://doi.org/10.1007/BF00436463

Guatam, A., Sharma, S., Bhadauria, R. 2009. Detection of toxigenic fungi and mycotoxins in medicinally important powdered herbal drugs. Internet Journal of Microbiology, vol. 7, no. 2. p. 1-8. DOI: https://doi.org/10.5580/104b

Kakalíková, L., Jankura, E., Šrobárová, A. 2009. First report of Alternaria bunch rot of grapevines in Slovakia. Australasian Plant Disease Notes, vol. 4, p. 68-69.

Labuda, R., Tančinová, D. 2006. Fungi recovered from Slovakian poultry feed mixtures and their toxinogenity. Annals of Agricultural and Environmental Medicine, vol. 13, p. 193-200.

Lau, B. P. Y., Scott, P. M., Lewis, D. A., Kanhere, S. R., Cléroux, C., Roscoe, V. A. 2003. Liquid chromatography–mass spectrometry and liquid chromatography–tandem mass spectrometry of the Alternaria mycotoxins alternariol and alternariol monomethyl ether in fruit juices and beverages. Journal of Chromatography A, vol. 998, no. 1-2, p. 119-131. https://doi.org/10.1016/s0021-9673(03)00606-x DOI: https://doi.org/10.1016/S0021-9673(03)00606-X

Lawrence, D. P., Gannibal, P. B., Peever, T. L., Pryor, B. M. 2013. The sections of Alternaria: formalizing species-group concepts. Mycologia, vol. 105, no. 3, p. 530-546. https://doi.org/10.3852/12-249 DOI: https://doi.org/10.3852/12-249

Lawrence, D. P., Rotondo, F., Gannibal, P. B. 2015. Biodiversity and taxonomy of the pleomorphic genus Alternaria. Mycological Progress, vol. 15, no. 1, p. 1-22, https://doi.org/10.1007/s11557-015-1144-x DOI: https://doi.org/10.1007/s11557-015-1144-x

Logrieco, A., Moretti, A., Solfrizzo, M. 2009. Alternaria toxins and plant diseases: an overview of origin, occurrence and risks. World Mycotoxin Journal, vol. 2, no. 2, p. 129-140. https://doi.org/10.3920/wmj2009.1145 DOI: https://doi.org/10.3920/WMJ2009.1145

Mašková, Z., Tančinová, D., Barboráková, Z., Mokrý, M. 2011. Frequented species of field fungi on wheat and their potential production of toxic metabolites. Potravinarstvo, vol. 5, no. 1, p. 43-50. https://doi.org/10.5219/108 DOI: https://doi.org/10.5219/108

Mašková, Z., Tančinová, D., Barboráková, Z., Felšöciová, S., Císarová, M. 2012. Comparison of occurrence and toxinogenity of Alternaria spp. isolated from samples of conventional and new crossbred wheat of Slovak origin. Journal of Microbiology, Biotechnology and Food Sciences, vol. 1, no. 4, p. 552-562.

Mašková, Z., Tančinová, Z., Rybárik, Ľ., Felšöciová, S., Kačinová, J. 2013. Colonization of grape berries by representatives of the genus Alternaria and their occurrence in the stum. Journal of Microbiology, Biotechnology and Food Sciences, vol. 2, no. 1, p. 1754-1764.

Ostrý, V. 2008. Alternaria mycotoxins: an overview of chemical characterization, producers, toxicity, analysis and occurrence in foodstuffs. World Mycotoxin Journal, vol. 1, no. 2, p. 175-188. https://doi.org/10.3920/wmj2008.x013 DOI: https://doi.org/10.3920/WMJ2008.x013

Patriarca, A. 2016. Alternaria in food products. Current Opinion in Food Science, vol. 11, p. 1-9. https://doi.org/10.1016/j.cofs.2016.08.007 DOI: https://doi.org/10.1016/j.cofs.2016.08.007

Patriarca, A., Vaamonde, G., Pinto, V. F. 2014. Alternaria. Encyclopedia of Food Microbiology, p. 54-60. https://doi.org/10.1016/B978-0-12-384730-0.00007-0 DOI: https://doi.org/10.1016/B978-0-12-384730-0.00007-0

Polizzotto, R., Andersen, B., Martini, M., Grisan, S., Assante, G., Musetti, R. 2012. A polyphasic approach for the characterization of endophytic Alternaria strains isolated from grapevines. Journal of Microbiological Methods, vol. 88, no. 1, p. 162-171. https://doi.org/10.1016/j.mimet.2011.11.009 DOI: https://doi.org/10.1016/j.mimet.2011.11.009

Pose, G., Patriarca, A., Kyanko, V., Pardo, A., Fernández Pinto, V. 2010. Water activity and temperature effects on mycotoxin production by Alternaria alternata on a synthetic tomato medium. International Journal of Food Microbiology, vol. 142, no. 3, p. 348-353. https://doi.org/10.1016/j.ijfoodmicro.2010.07.017 DOI: https://doi.org/10.1016/j.ijfoodmicro.2010.07.017

Prelle, A., Spadaro, D., Garibaldi, A., Gullino, M. L. 2013. A new method for detection of five Alternaria toxins in food matrices based on LC–APCI-MS. Food Chemistry, vol. 140, no. 1-2, p. 161-167. https://doi.org/10.1016/j.foodchem.2012.12.065 DOI: https://doi.org/10.1016/j.foodchem.2012.12.065

Samson, R. A., Hoekstra, E. S., Frisvad, J. C., Filtenborg, O. 2002a. Introduction to food- and airborne fungi. UTRECHT : Centraalbureau voor Schimmelcultures, 389 p. ISBN 90-70351-42-0.

Samson, R. A., Hoekstra, E. S., Lund, F., Filtenborg, O., Frisvad, J. C. 2002b. Method for the detection, isolation and characterisation of food-borne fungi. In Samson, R. A., Hoekstra, E. S., Frisvad, J. C., Filtenborg, O. Introduction to food- and airborne fungi. Utrecht, Netherland : Centraalbureau voor Schimmecultures, p. 283-297. ISBN 90-70351-42-0.

Simmons, E. G. 1992. Alternaria taxonomy: current status, viewpoint, challenge. In Chielkowski, J., Visconti, A. Alternaria: biology, plant diseases, and metabolites. NEW YORK, USA : Elsevier, p. 1-35. ISBN-13 978-0444889980.

Simmons, E. G. 1994. Alternaria themes and variations (106 – 111). Mycotaxon, vol. 50, p. 409-427.

Simmons, E. G. 2007. Alternaria, An Identification Manual. Utrecht, Netherland : CBS Fungal Biodiversity Centre, 775 p. ISBN 978-90-70351-68-7.

Simmons, E. G. Roberts R. G. 1993. Alternaria themes and variations (73). Mycotaxon, vol. 40, p. 109-140.

Swart, A. E., Holz, G. 2017. Colonization of Table Grape Bunches by Alternaria alternata and Rot of Cold-Stored Grapes. South African Journal of Enology & Viticulture, vol. 15, no. 2, p. 19-25. https://doi.org/10.21548/15-2-2280 DOI: https://doi.org/10.21548/15-2-2280

Tančinová, D., Kačániová, M., Javoreková, S. 2001. Natural occurrence of fungi in feeding wheat after harvest and during storage in the agricultural farm facilities. Biologia, vol. 56, no. 3, p. 247-250.

Tančinová, D., Labuda, R. 2009. Fungi on wheat bran and their toxinogenity. Annals of Agricultural and Environmental Medicine, vol. 16, no. 2, p. 325-31.

Woudenberg, J. H. C., Groenewald, J. Z., Binder, M., Crous, P. W. 2013. Alternaria redefined. Studies in Mycology, vol. 75, p. 171-212. https://doi.org/10.3114/sim0015 DOI: https://doi.org/10.3114/sim0015

Zwickel, T., Klaffke, H., Richards, K., Rychlik, M. 2016. Development of a high performance liquid chromatography tandem mass spectrometry based analysis for the simultaneous quantification of various Alternaria toxins in wine, vegetable juices and fruit juices. Journal of Chromatography A, vol. 1455, p. 74-85. https://doi.org/10.1016/j.chroma.2016.04.066 DOI: https://doi.org/10.1016/j.chroma.2016.04.066

Published

2019-06-28

How to Cite

Mašková, Z., Tančinová, D., & Ballová, M. (2019). Alternaria SPP. in food commodities of Slovak origin: occurrence and mycotoxin production abilities. Potravinarstvo Slovak Journal of Food Sciences, 13(1), 524–531. https://doi.org/10.5219/1102

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