Expression pattern of thaumatin in the selected red varieties of Vitis vinifera, L.
DOI:
https://doi.org/10.5219/1057Keywords:
thaumatin, expression, red varieties, Vitis vinifera L.Abstract
Vitis vinifera L. is a specie that is adapted to a very variable range of climates, from cold up to the desert one, but especially it grows in the temperate Mediterranean regions and continental areas of Europe. Grape is a widespread consumed fruit as well as processed to musts, juices or wine. The health beneficial effects of grapes and wine are very well known due to their high nutritional value and unique phytochemical composition. Despite many health protective and beneficial effects of Vitis vinifera, a part of population suffer to allergic reactions to this fruit. Allergens of wine and grapes are: endochitinases, lipid-transfer protein and thaumatin. Thaumatin is a protein having a sweet taste belonging to the PR5-like proteins. These proteins are very difersified in their functions and were described to be involved in stress responses and fruit ripening, but are expressed in healthy grape fruits in a constitutive manner and needn´t to be expressed only as a answer to the stress. Thaumatin is a minor allergen in grape, but belonging to the suspected panallergens relevant to the food cross-allergy induction, its importance is quite high. Another importance of this protein is a technological one, as reported to aggregate in wine to form a visible haze unless removed prior to bottling. In this study, expression of thaumatin-like allergen was analysed in the grapes of selected varieties. Grapes of four red varieties of Vitis vinifera, L. were obtained in the season 2017 in the Sabo winery that belongs to the Malokarpatská wine region. Fresh maturated grapes of varieties Alibernet, Cabernet Sauvignon , Frankovka modrá and Dornfelder were analysed. Expression changes of thaumatin was calculated by delta delta Ct method. Dornfelder was found as to have the lowest activity in thaumatin-like gene activity, mainly when comparing to the Cabernet Sauvignon and Frankovka modrá. Alibernet, on the other side, has the expression level of thaumatin very similar when comparing to the Cabernet Sauvignon and Frankovka modrá.
Downloads
Metrics
References
Ahmad, F., Khan, G. M. 2012. Study of aging and hepatoprotective activity of Vitis Vinifera L. seeds in albino rats. Asian Pacific Journal of Tropical Biomedicine, vol. 2, no. 3, p. 1770-1774. https://doi.org/10.1016/S2221-1691(12)60492-4 DOI: https://doi.org/10.1016/S2221-1691(12)60492-4
Andrade, S., Ramalho, M. J., Pereira, M. C., Loureiro, J. A. 2018. Resveratrol Brain Delivery for Neurological Disorders Prevention and Treatment. Front Pharmacol, vol. 9, 1261 p. https://doi.org/10.3389/fphar.2018.01261 DOI: https://doi.org/10.3389/fphar.2018.01261
Arora, P., Ansari, S. H., Najmi, A. K., Anjum, V., Ahmad, S. 2016. Investigation of anthi-asthmatic potential of dried fruits of Vitis vinifera L. in animal model of bronchial asthma. Allergy, Asthma and Clinical Immunology, vol. 12, 42 p. https://doi.org/10.1186/s13223-016-0145-x DOI: https://doi.org/10.1186/s13223-016-0145-x
Barthomeuf, C., Lamy, S., Blanchette, M., Boivin, D., Gingras, D., Beliveau, R. 2006. Inhibition of sphingosine-1-phosphate- and vascular endothelial growth factor-induced endothelial cell chemothaxis by red grape skin polyphenols correlates with a decrease in early platelet-activating factor synthesis. Free Radical Biology and Medicine, vol. 40, no. 4, p. 581-590. https://doi.org/10.1016/j.freeradbiomed.2005.09.015 DOI: https://doi.org/10.1016/j.freeradbiomed.2005.09.015
Bircher, A. J., Bigliardi, P., Yilmaz, B. 1999. Anaphylaxis resulting from selective sensitization to Americana grapes. Journal of Allergy and Clinical Immunology, vol. 4, no. 5, p. 1111-1113. https://doi.org/10.1016/S0091-6749(99)70099-0 DOI: https://doi.org/10.1016/S0091-6749(99)70099-0
Breiteneder, H. 2004 Thaumatin-like proteins – a new family of pollen and fruit allergens. Allergy, vol. 59, no. 5, p. 479-481. https://doi.org/10.1046/j.1398-9995.2003.00421.x DOI: https://doi.org/10.1046/j.1398-9995.2003.00421.x
Brito, F. F., Gimeno, P. M., Bartolome, B., Alonso, A. M., Lara, P., Fernandez, J. A., Martinez, A. 2008. Vine pollen allergy in areas with a high density of vineyards. Annals of Allergy, Asthma and Immunology, vol. 100, no. 6, p. 596-600. https://doi.org/10.1016/S1081-1206(10)60054-3 DOI: https://doi.org/10.1016/S1081-1206(10)60054-3
Cianferoni, A., Spergel, J. M. 2009. Food Allergy: Review, Classification and Diagnosis. Allergology International, vol. 58, no. 4, p. 457-466. https://doi.org/10.2332/allergolint.09-RAI-0138 DOI: https://doi.org/10.2332/allergolint.09-RAI-0138
Cui, J., Juhasz, B., Tosaki, A., Maulik, N., Das, D. K. 2002. Cardioprotection with grapes. Journal of Cardiovascular Pharmacology, vol. 40, no. 5, p. 762-769. https://doi.org/10.1097/00005344-200211000-00014 DOI: https://doi.org/10.1097/00005344-200211000-00014
Ferreira, R. B., Piçarra-Pereira, M. A., Monteiro, S., Loureiro, V. B., Teixeira, A. R. 2001. The wine proteins. Trends Food Sci Technol, vol. 12, no. 7, p. 230-239. https://doi.org/10.1016/S0924-2244(01)00080-2 DOI: https://doi.org/10.1016/S0924-2244(01)00080-2
Giannoccaro, F., Munno, G., Riva G., Pugliese, S., Paradiso, M. T., Ferrannini, A. 1998. Oral allergy syndrome to grapes. Allergy, vol. 53, no. 4, p. 451-452. https://doi.org/10.1111/j.1398-9995.1998.tb03923.x DOI: https://doi.org/10.1111/j.1398-9995.1998.tb03923.x
Charron, C., Giegé, R., Lorber, B. 2003 Structure of thaumatin in a hexagonal space group: comparison of packing contacts in four crystal lattices. Acta Crystallogr Sect D Biol Crystallogr, vol. 60, p. 83-89. https://doi.org/10.1107/S0907444903022613 DOI: https://doi.org/10.1107/S0907444903022613
Kalogeromitros, D. C., Makris, M. P., Gregoriou, S. G., Katoulis, A. C., Straurianeas, N. G. 2006. Sensitization to other foods in subjects with reported allergy to grapes. Allergy and Asthma Proceedings, vol. 27, no. 1, p. 68-71. https://doi.org/10.2500/aap.2006.27.2882 DOI: https://doi.org/10.2500/aap.2006.27.2882
Livak, K. J., Schmittgen, T. D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, vol. 25, no. 4, p. 402-408. https://doi.org/10.1006/meth.2001.1262 DOI: https://doi.org/10.1006/meth.2001.1262
Marangon, M., Van Sluyter, S. C., Waters, E. J., Menz, R. I. 2014. Structure of Haze Forming Proteins in White Wines: Vitis vinifera Thaumatin-Like Proteins. PLOS, https://doi.org/10.1371/journal.pone.0113757 DOI: https://doi.org/10.2210/pdb4jru/pdb
Masani, Y. A., Mathew, N., Chakraborty, M., Kamath, J. V. 2012. Effects of Vitis vinifera against Trition-X-100 induced hyperlipidaemia in rats. Inter Res J Pharm, vol. 3, no. 12, p.101-103.
Palacín, A., Tordesillas, L., Gamboa, P., Sánchez-Monge, R., Cuesta-Herranz, J., Sanz, M. L., Barber, D., Salcedo, G., Díaz-Perales, A. 2010 Characterization of peach thaumatin-like proteins and their identification as major peach allergens. Clinical and Experimental Allergy, vol. 40, no. 9, p. 1422-1430. https://doi.org/10.1111/j.1365-2222.2010.03578.x DOI: https://doi.org/10.1111/j.1365-2222.2010.03578.x
Pastorello, E. A., Farioli, L., Pravettoni, V., Ortolani, C., Fortunato, D., Giuffrida, M. G., Perono Garoffo, L., Calamari, A. M., Brenna, O., Conti, A. 2003. Identification of grape and wine allergenes as an endochitinase 4, a lipid-transfer protein, and a thaumatin. Journal of Allergy and Clinical Immunology, vol. 111, no. 2, p. 350-359. https://doi.org/10.1067/mai.2003.35 DOI: https://doi.org/10.1067/mai.2003.35
Senna, G., Mistrello, G., Roncarolo, D., Crivellaro, M., Bonadonna, P., Schiappoli, M., Passalacgua, G. 2001. Exercise-induced anaphylaxis to grape. Allergy, vol. 56, no. 12, p. 1235-1236. https://doi.org/10.1034/j.1398-9995.2001.00352.x DOI: https://doi.org/10.1034/j.1398-9995.2001.00352.x
Smyth, G. K. 2004. Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Statistical Applications in Genetics and Molecular Biology, vol. 3, no. 1, p. 1-25. https://doi.org/10.2202/1544-6115.1027 DOI: https://doi.org/10.2202/1544-6115.1027
Social Science Statistics. 2019. T-Test Calculator for 2 Independent Means. Available at: www.socscistatistics.com/tests/studentttest
Varoni, E. M., Lo Faro, A. F., Sharifi-Rad, J., Iriti, M. 2016. Anticancer Molecular Mechanisms of Resveratrol. Front Nutr, vol. 3, p. 1-15. https://doi.org/10.3389/fnut.2016.00008 DOI: https://doi.org/10.3389/fnut.2016.00008
Vassilopoulou, E., Zuidmeer, L., Akkerdaas, J., Tassios, I., Rigby, N. R., Mills, E. N., van Ree, R., Saxoni-Papageorgiou, P., Papadopoulos, N. G. 2007. Severe immediate allergic reactions to grapes: part of a lipid transfer protein-associated clinical syndrome. International Archives of Allergy and Immunology, vol. 143, no. 2, p. 92-102. https://doi.org/10.1159/000098657 DOI: https://doi.org/10.1159/000098657
Wang, Q., Li. F., Zhang, X., Zhang, Y., Hou, Y., Zhang, S., Wu, Z. 2011. Purification and characterization of a CkTLP protein from Cynanchum komarovii seeds that confers antifungal activity. In PLoS One, vol. 22, no. 6, e16930. https://doi.org/10.1371/journal.pone.0016930 DOI: https://doi.org/10.1371/journal.pone.0016930
Waters, E. J., Wallace, W., Williams, P. J. 1991. Haat haze characteristics of fractionated wine proteins. Am J Enol Vitic vol. 42, p. 123-127.
Yan,X., Qiao, H., Zhang, H., Guo, Ch., Wang, M., Wang, Y., Wang, X. 2017. Analysis of the grape (Vitis vinifera L.) thaumatin-like protein (TLP) gene family and demonstration that TLP29 contributes to disease resistance. Scientific Reports, vol. 7, no. 4269. https://doi.org/10.1038/s41598-017-04105-w DOI: https://doi.org/10.1038/s41598-017-04105-w
Žiarovská, J., Zeleňáková, L., Fernandéz, E. C., Kačániová, M. 2018. A thaumatin-like genomic sequence identification in Vitis vinifera L., stormy wines and musts based on direct PCR. Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, p. 226-232. https://doi.org/10.5219/892 DOI: https://doi.org/10.5219/892
Published
How to Cite
Issue
Section
License
This license permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
