Tibia mineralization of chickens determined to meat production using a microbial phytase


  • Mária Angelovičová Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra
  • Martin Mellen Klas s.r.o., Osloboditeľov 66, 990 01 Veľký Krtí­š
  • Peter Zajác Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra
  • Jozef Čapla Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra
  • Marek Angelovič Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Machines and Production Systems, Tr. A. Hlinku 2, 949 76 Nitra




broiler, microbial phytase, additive, phosphorus, tibia, mineralization


The target of the research was 6-phytase of microbial origin. It was used in feed mixtures for chickens determined to meat production. Its effect has been studied in relation to the tibia mineralization by calcium, phosphorus and magnesium. 6-phytase is a product of Aspergillus oryzae. That was obtained by means of biotechnological processes of production of commercially available enzymes. It was incorporated in the feed mixtures 0.1%. In a 38-day feeding trial, 300 one-day-old, as hatched, Cobb 500 chickens determined to meat production (100 birds per group) were fed on one concentrations of dietary non-phytate phosphorus (2.32, 2.31 g.kg-1, respectively and supplemental microbial phytase (0 and 500   FTU.kg-1 feed mixtures). Control group was used to compare the results and control feed mixtures contained 4.5 g.kg-1 without microbial phytase. At days 40 it was selected 6 birds in every group, which were slaughter in accordance with the principles of welfare. Left tibias of every bird were used to determination of calcium, phosphorus and magnesium contents. According to in vivo, it was found that the addition of microbial phytase to reduced dietary non-phytate phosphorus increased concentrations of calcium (Ca), phosphorus (P) and magnesium (Mg) in tibia. The differences among groups were statistically significant (p <0.05). It was concluded that reducing of dietary non-phytate phosphorus on the 2.32, 2.31 g.kg-1, respectively, by monocalcium phosphate and microbial phytase supplementation in feed mixtures facilitated tibia mineralization at chicken determined to meat production.


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How to Cite

Angelovičová, M. ., Mellen, M. ., Zajác, P. ., Čapla, J. ., & Angelovič, M. . (2018). Tibia mineralization of chickens determined to meat production using a microbial phytase. Potravinarstvo Slovak Journal of Food Sciences, 12(1), 40–49. https://doi.org/10.5219/805

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