The content of total polyphenols, ascorbic acid and antioxidant activity in selected varieties of quince (Cydonia oblonga mill.)

Judita  Bystrická; Janette  Musilová; Helena  Lichtnerová; Marianna  Lenková; Ján  Kovarovič; Marek  Chalas

doi:10.5219/699

Authors

Judita Bystrická Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Chemistry, Tr. A. Hlinku 2, 949 76 Nitra
Janette Musilová Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Chemistry, Tr. A. Hlinku 2, 949 76 Nitra
Helena Lichtnerová Slovak University of Agriculture in Nitra, Horticulture and Landscape Engineering Faculty, Department of Planting Design and Maintenance, Tulipánová 7, 949 76 Nitra
Marianna Lenková Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Chemistry, Tr. A. Hlinku 2, 949 76 Nitra
Ján Kovarovič Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Chemistry, Tr. A. Hlinku 2, 949 76 Nitra
Marek Chalas Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Chemistry, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/699

Keywords:

quince fruit, total polyphenol, ascorbic acid, variety

Abstract

Quince fruit (Cydonia oblonga Miller) is an important source of bioactive compounds, especially of polyphenolic compounds, phenolic acids, flavonoids also of minerals and vitamins. This compounds exhibit health promoting properties including antibacterial, anti-inflammatory, anticancer, antidiabetic and cardioprotective properties. Quine fruit have a high therapeutic value, can be used as good sources of antioxidants. This study provides some knowledge about content of total polyphenols, ascorbic acid and antioxidant activity in selected varieties of quince fruit samples. Four quince fruit cultivars (Semenáč, Konstantinopler Apfelquitte, Cydora Robusta, Mammut) were analysed. The content of the total polyphenols (TPC) was determined by the Folin-Ciocalteu reagent (FCR) at 765 nm using spectrophotometer. Ascorbic acid (AsA) content was determined using standard HPLC gradient method. Antioxidant activity (AA) was measures using a compound DPPH˙(2.2-diphenyl-1-picrylhydrazyl). The content of (TPC) in fresh samples of quince fruit ranged from 661 ±11.60 mg.kg^-1 to 1044 ±11.03 mg.kg^-1 and content of AsA were in interval from 151 ±0.58 mg.kg^-1 to 215 ±0.75 mg.kg^-1. The values of antioxidant activity in quince fruit samples were in range from 26.90 ±0.61%to 49.14 ±0.38%. Statistically significant highest content TPC, AsA and AA was recorded in cultivar Konstaninopler Apfelquitte and statistically lowest content was recorded in cultivar Semenáč. The content of TPC, AsA and AA beside the variety may be affected by many factors also climatic conditions and the agrochemical composition of the soil.

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References

Al-Snafi, A. E. 2016. The medical importance of Cydonia oblonga - A review. Journal of Pharmacy, vol. 6, no. 6, p. 87-99.

Ashraf, M. U., Muhammad, G., Hussain, M. A., Bukhari, S. A. A. 2016. Cydonia oblonga M., A Medicinal plant rich in phytonutrients for pharmaceuticals. Frontiers in Pharmacology, vol. 7, p. 163.

Benzarti, S., Hamdi, H., Lahmayer, I., Toumi, W., Kerkeni, A., Belkadhi, K., Sebei, H. 2015. Total phenolic compounds and antioxidant potential of quince (Cydonia oblonga Miller) leaf methanol extract. International Journal of Innovation and Applied Studies, vol. 13, no. 3, p. 518-526.

Brand-Williams, W., Cuvelier, M. E., Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft and Technologie, vol. 28, no. 1, p. 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5

Costa, R. M., Magalhăes, A. S., Pereira, J. A., Andrade, P. B., Valentăo, P., Carvalho, M., Silva, B. M. 2009. Evaluation of free radical-scavenging and antihemolytic activities of quince (Cydonia oblonga) leaf : A comparative study with green tea (Camellia sinensis). Food and Chemical Toxicology, vol. 47, no. 4, p. 860-865. https://doi.org/10.1016/j.fct.2009.01.019

Drogoudi, P. D., Michailidis, Z., Pantelidis, G. 2008. Peel and flesh antioxidant content and harvest quality characteristics of seven apple cultivars. Scientia Horticulturae, vol. 115, no. 2, p. 149-153. https://doi.org/10.1016/j.scienta.2007.08.010

Fattouch, S., Caboni, P., Coroneo, V., Tuberoso, C. I. G., Angioni, A., Dessi, S., Marzouki, N., Cabras, P. 2007. Antimicrobial activity of Tunisian quince (Cydonia oblonga Miller) pulp and peel polyphenolic extracts. Journal of Agricurtural and Food Chemistry, vol. 55, no. 3, p. 963-969. https://doi.org/10.1021/jf062614e

Gheisari, H. R., Abhari, K. H. 2014. Drying method effects on the antioxidant activity of quince (Cydonia oblonga Miller) tea. Acta Scientarum Polonorum Technologia Alimentaria, vol. 13, no. 2, p. 129-134. https://doi.org/10.17306/J.AFS.2014.2.2

Hopur, H., Asrorov, A. M., Qingling, M., Yili, A., Ayupbek, A., Nannan, P., Aisa, H. A. 2011. HPLC analysis of polysaccharides in quince (Cydonia oblonga Mill. var. maliformis) fruit and PTP1B inhibitory activity. The Natural Product Journal, vol. 1, no. 2, p. 146-150. https://doi.org/10.2174/2210315511101020146

Lachman, J., Proněk, D., Hejtmánková, A., Dudjak, J., Pivec, V., Faitová, K. 2003. Total polyphenol and main flavonoid antioxidants id different onion (Allium cepa L.) varieties. Horticultural Science, vol. 30, p. 142-147.

Mir, S. A., Masoodi, F. A., Gani, A., Ganaie, S. A., Reyaz, U., Wani, S. M. 2015. Evaluation of antioxidant properties of methanolic extracts from different fractions of quince (Cydonia oblonga Miller). Advances in Biomedicine and Pharmacy, vol. 2, no. 1, p. 1-6. https://doi.org/10.19046/abp.v02i01.01

Pacifico, S., Gallicchio, M., Fiorentino, A., Fischer A., Meyer, U., Stintzing. 2012. Antioxidant properties and cytotoxic effects on human cancer cell lines of aqueous fermented and lipophilic quince (Cydonia oblonga Mill.) preparations. Food and Chemical Toxicology, vol. 50, no. 11, p. 4130-4135. https://doi.org/10.1016/j.fct.2012.07.061

Rop, O., Balik, J., Řezniček, V., Jurikova, T., Škardova, P., Salaš, P., Sochor, J., Mlček, J., Kramařova, D. 2011. Chemical characteristic of fruits of some selected quince (Cydonia oblonga Mill.) cultivars. Czech Journal of Food Sciences, vol. 29, no. 1, p. 65-73.

Sadeghpour, O., Alias, F., Toliyat, T., Mohammadi, A., Minaee, B., Samadi, N., Aliasl, J. 2016. Medicinal properties of Cydonia oblonga Mill fruit (Pulp and Peel) in Iranian traditional medicine and modern phytotherapy. Traditional and Integrative Medicine, vol. 1, no. 3, p. 122-128.

Sharma, R., Joshi, V. K., Rana, J. C. 2011. Nutritional composition and processed products of quince (Cydonia oblonga Mill.). Indian Journal of Natural Products and Resources, vol. 2, no. 3, p. 354-357.

Silva, B. M., Andrade, P. B., Mendes, G. C., Seabra, R. M., Ferreira, M. A. 2002. Study of the organic acids composition of quince (Cydonia oblonga Miller) fruit and Jam. Journal of Agricultural and Food Chemistry, vol. 50, no. 8, p. 2313-2317. https://doi.org/10.1021/jf011286

Silva, B. M., Andrade, P. M., Ferreres, F., Seabra, R. M., Oliviera, M. B., Ferreira, M. A. 2005. Composition of quince (Cydonia oblonga Miller) seeds: Phenolic, organic acids and free amino acids. Natural Product Research, vol. 19, no. 3, p. 275-281. https://doi.org/10.1080/14786410410001714678

Silva, B. M., Valentăo, P., Seabra, R. M., Andrade, P. B. 2008. Quince (Cydonia oblonga Miller): an interesting dietary source of bioactive compounds. In Papadopoulos, K. N. Food Chemistry research developments. Ed. K. N. Papadopoulos. New York : Nova Science Publishers, p. 243-266. ISBN 978-1-60456-262-0.

Souci, S. W., Fachmann, W., Kraut, H. 2008. Food composition and nutrition tables. 7th ed. Stuttgart, Germany : CRC Press, MedPharm, Scientific Publishers. 1400 p. ISBN-978-3-8047-5038-8.

Trigueros, L., Pérez-Alvarez, J. A., Viuda-Martos, M., Sendra, E. 2011.Production of low-fat yogurt with quince (Cydonia oblonga Mill.) scalding water, LWT - Food Science and Technology, vol. 44, no. 6, p. 1388-1395. https://doi.org/10.1016/j.lwt.2011.01.012

Wani, T. A., Wani, S. M., Shah, A. G., Masoodi, F. A. 2013. Optimizing conditions for antioxidant extraction from Sea Buckthorn leaf (Hippophae rhamnoides L.) as herbal tea using response surface methodology (RSM). International Food Research Journal, vol. 20, no. 1 p. 1677-1681. https://doi.org/10.1080/23311932.2015.1128519

Wojdylo, A., Oszmiański, J., Bielicki, P. 2013. Polyphenolic composition, antioxidant activity, and polyphenol oxidase (PPO) activity of quince (Cydonia oblonga Miller) varieties. Journal of Agricultural and Food Chemistry, vol. 61, no. 11, p. 2762-2772. https://doi.org/10.1021/jf304969b

URL 1 Available at: http://www.prodejstromku.cz/produkt/amanliska

URL 2 Available at: http://web03.bruns.de/bruns/en/EUR//Pflanzen/QUITTE-%27Konstantinopler-Apfelquitte%27/p/4605

URL 3 Available at: http://ovocnedreviny.sk/produkt/jadroviny/dula/dula-mammut/

URL 4 Available at: http://gyumolcspedia.hu/cydora-robusta-birs