Lunasin detection in coloured wheat genotype


  • Milan Chňapek Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Biochemistry and Biotechnology, Tr. A. Hlinku 2, 949 76 Nitra
  • Dušan Siman Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Biochemistry and Biotechnology, Tr. A. Hlinku 2, 949 76 Nitra
  • Zdenka Gálová Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Biochemistry and Biotechnology, Tr. A. Hlinku 2, 949 76 Nitra



cancer, coloured wheat, gene detection, lunasin, PCR


Lunasin is a biologically active protein, composed of 43 amino acid residues. There has been proven many health-promoting effects of lunasin peptide. The most important health benefits include: anti-hypertension, antioxidant activity, cancer prevention or therapy. It was also demonstrated anti-inflammation, hypocholesterolemic activity, anti-obesity and immunomodulation. The focus of our research is to summarize the discovery, characterization and biological activities of lunasin, which will provide a reference for the future development and utilization of lunasin, and a basis for exploring the underlying mechanisms of these health-beneficial functions. Lunasin was first isolated in 1987 at Niigata University School of Medicine in Japan, during the screening of protease inhibitors from soybean seeds. It was subsequently found in other beans, grains and herbal plants, including wheat, barley, rye, triticale. Concentration of lunasin is ranging from 0.013 to 70.5 mg protein lunasin/g of protein. Big step forward in the understanding of the lunasine operating mechanism in the fight against cancer has arisen after study on cloning of the soybean lunasin gene and subsequent transfection into mammalian cells which led to the discovery that the lunasin gene can disrupt mitosis and induce chromosome breakage, ultimately leading to cell apoptosis. The main goal of our work was to evaluate collection of wheat with unusual grain colour for presence of lunasin gene. DNA was extracted by commercial kit and lunasin gene was detected by PCR reaction. Our results showed presence of lunasin gene detected by 3 combinations of 2 sets of primer pair and indicated lunasin peptide presence in cereal grains. These findings are necessary to confirmed by proteome analysis.


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Bassam, B. J, Caetano-Anollés, G., Gresshoff, P. M. 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry, vol. 196, no. 1, p. 80-83. DOI:

Chang, H. C., Lewis, D., Tung, C. Y., Han, L., Henriquez, S. M. P., Voiles, L., Lupov, I. P., Pelloso, D., Sinn, A. L., Pollok, K. E., de Lumen, B. O., Li, F., Blum, J. S., Srivastava, S., Robertson, M. J. 2013. Soypeptide lunasin in cytokine immunotherapy for lymphoma. Cancer Immunol Immunother, vol. 63, no. 3, p. 283-295. PMid:24363024 DOI:

Dinelli, G., Bregola, V., Bosi, S., Fiori, J., Gotti, R., Simonetti, E., Trozzi, C., Leoncini, E., Prata, C., Massaccesi, L., Malaguti, M., Quinn, R., Hrelia, S. 2014. Lunasin in wheat: A chemical and molecular study on its presence or absence. Food Chemistry, vol. 151, p. 520-255. PMid:24423565 DOI:

Hernández-Ledesma, B., Hsieh, C. C., de Lumen, B. O. 2009. Lunasin, a novel seed peptide for cancer prevention. Peptides, vol. 30, no. 2, p. 426-430. PMid:19056440 DOI:

Hsieh, C. C., Hernández-Ledesma, B., de Lumen, B. O. 2010. Lunasin, a novel seed peptide, sensitizes human breast cancer MDA-MB-231 cells to aspirin-arrested cell cycle and induced apoptosis. Chemico-Biological Interactions, vol. 186, no. 2, p. 127-134. PMid:20457246 DOI:

Jeong, H. J., Jeong, J. B., Hsieh, C. C., Hernández-Ledesma, B., de Lumen, B. O. 2010. Lunasin is prevalent in barley and is bioavailable and bioactive in In Vivo and In Vitro studies. Nutrition and Cancer, vol. 62, no. 8, p. 1113-1119. PMid:21058199 DOI:

Jeong, H. J., Jeong, J. B., Kim, D. S., Park, J. H., Lee, J. B., Kweon, D. H., Chung, G. Y., Seo, E. W., de Lumen, B. O. 2007. The cancer preventive peptide lunasin from wheat inhibits core histone acetylation. Cancer Letters, vol. 255, no. 1, p. 42-48. PMid:17481808 DOI:

Jeong, H. J., Lee, J. R., Jeong, J. B., Park, J. H., Cheong, Y., de Lumen, B. O. 2009. The cancer preventive seed peptide lunasin from rye is bioavailable and bioactive. Nutrition and Cancer, vol. 61, no. 5, p. 680-686. PMid:19838942 DOI:

Kyle, S., James, K. A. R., McPherson, M. J. 2012. Recombinant production of the therapeutic peptide lunasin. Microbial Cell Factories, vol. 11, no. 28. DOI:

Lumen, B. O. 2005. Lunasin: A cancer-preventive soy peptide. Nutrition Reviews, vol. 63, no. 1, p. 16-21. PMid:15730231 DOI:

Maldonado-Cervantes, E., Jeong, H. J., León-Galván, F., Barrera-Pacheco, A., De León-Rodríguez, A., González de Mejia, E., de Lumen, B. O., Barba de la Rosa, A. P. 2010. Amaranth lunasin-like peptide internalizes into the cell nucleus and inhibits chemical carcinogen-induced transformation of NIH-3T3 cells. Peptides, vol. 31, no 9, p. 1635-1642. PMid:20599579 DOI:

Mitchell, R. A. C., Lovegrove, A., Shewry, P. R. 2013. Lunasin in cereal seeds: What is the origin? Journal of Cereal Science, vol. 57, no. 3, p. 267-269. PMid:24817784 DOI:

Nakurte, I., Kirhnere, I, Namniece, J., Saleniece, K., Krigere, L., Mekss, P., Vicupe, Z., Bleidere, M., Legzdina, L., Muceniece, R. 2013. Detection of the lunasin peptide in oats (Avena sativa. L). Journal of Cereal Science, vol. 57, no. 3, p. 319-324. DOI:

Nakurte, I., Klavins, K., Kirhnere, I., Namniece, J., Adlere, L., Matvejevs, J., Kronberga, A., Kokare, A., Strazdina, V., Legzdina, L., Muceniece, R. 2012. Discovery of lunasin peptide in triticale (X Triticosecale Wittmack). Journal of Cereal Science, vol. 56, no. 2, p. 510-514. DOI:

Wang, W., Dia, V. P., Vasconez, M., de Meija E. G., Nelson, R. L. 2008. Analysis of soybean protein-derived peptides and the effect of cultivar, environmental conditions, and processing on lunasin concentration in soybean and soy products. Journal of AOAC International, vol. 91, no. 4, p. 936-946. PMid:18727556 DOI:




How to Cite

Chňapek, M. ., Siman, D. ., & Gálová, Z. . (2016). Lunasin detection in coloured wheat genotype. Potravinarstvo Slovak Journal of Food Sciences, 10(1), 152–156.

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