The testing of sanitizers efficacy to enterococci adhered on glass surfaces
DOI:
https://doi.org/10.5219/457Keywords:
enterococci, sanitation, glass, biofilmAbstract
The aim of this work was to test the ability of 6 strains of enterococci to adhere on glass surfaces in environment with different content of milk residues and then to evaluate efficacy of 2 commercial sanitizers (alkaline and acidic) used in milk production. Tested enterococci were isolated from milk, dairy products and from rinse water after sanitation milking machine. Suspension of enterococci (8 log CFU.ml-1) was prepared in phosphate buffered saline (PBS), PBS with content 0.1% and 1% of skimmed reconstituted milk. Glass plates were immersed into bacterial suspension for 1 h at 37 °C. The number of enterococci adhered on glass surface in PBS achieved an average value 3.47 log CFU.mm-2, in PBS with 0.1% of milk 2.90 CFU.mm-2, in PBS with 1% of milk 2.63 CFU.mm-2. Differences between the tested files were not statistically significant (p >0.05). In the second part of work the glass plates with adhered enterococci were exposed to the effect of alkaline sanitizer (on basis of NaOH and NaClO), respectively acidic sanitizer (on basis of H3PO4). Sanitation solutions were prepared and tested according to manufacturer recommendations (concentration 0.25%, contact time 20 min, temperature 20 °C). Alkaline sanitation solution was 100% effective against all tested enterococci regardless to content of milk residues in environment. Acidic sanitation solution was 100% effective only against E. faecalisD (isolated from rinse water after sanitation). Average value of reduction of enterococci with acidic sanitation solution, which were on glass plates in environment PBS was 2.84 CFU.mm-2, in PBS with 0.1% of milk was 2.45 CFU.mm-2 and in PBS with 1% of milk was2.16 CFU.mm-2. It can be concluded, that increase of milk residues in environment decrease the adhesion of enterococci on glass surface, but also effectiveness of acidic sanitation solution.
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References
Barbosa, J., Gibbs, P. A., Teixeira, P. 2010. Virulence factors among enterococci isolated from traditional fermented meat products in North of Portugal. Food Control, vol. 21,
p. 651-656. https://doi.org/10.1016/j.foodcont.2009.10.002
Barnes, L. M., Lo, M. F., Adams, M. R., Chamberlain, A. H. L. 1999. Effect of milk proteins on adhesion of bacteria to stainless steel surfaces. Applied and Environmental Microbiology, vol. 65, no. 10, p. 4543-4548. PMid:10508087
Carballo, J., Araújo, A. B. 2012. Evaluation of the efficacy of commercial sanitizers against adhered and planktonic cells of Listeria monocytogenes and Salmonella spp. Ciencia e Technologia de Alimentos, vol. 32, no. 3, p. 606-612. https://doi.org/10.1590/s0101-20612012005000084
Cassenego, A. P. V., d´Azevedo, P. A., Ribeiro, A. M. L., Frazzon, J., Van Der Sand, S. T., Frazzon, A. P. G. 2011. Species distribution and antimicrobial susceptibility of enterococci isolated from broilers infected experimentally with Eimeria spp and fed with diets containing different suplements. Brazilian Journal of Microbiology, vol. 42, no. 2, p. 480-488. https://doi.org/10.1590/S1517-83822011000200012 PMid:24031659
Dat, N. M., Hamanaka, D., Tanaka, F., Uchino, T. 2010. Surface conditioning of stainless steel coupons with skim milk solutions at different pH values and its effect on bacterial adherence. Food Control, vol. 21, p. 1769-1773. https://doi.org/10.1016/j.foodcont.2010.06.012
Ducková, V., Čanigová, M., Kročko, M., Lavová, M. 2014a. Antibiotic susceptibility and biofilm forming capacity of enterococci isolated from food of animal origin. Medycyna weterynaryjna, vol. 70, no. 1,
p. 36-37. [cit. 2015-01-15] Available at: http://medycynawet.edu.pl/images/stories/pdf/pdf2014/012014/201401036037.pdf
Ducková, V., Kročko, M., Čanigová, M., Bezeková, J., Lavová, M., Kňazovická, V., Krebs-Artimová, A. 2014b. The evaluation of enterococci ability to form biofilm and survive the process of sanitation in vitro. Technologia produkcji i bezpieczeństwo żywności, Polskie Towarzystwo Technológów Żywności – Kraków, p. 110-118, ISBN 978-83-937001-3-4.
Folquie Moreno, M. R., Sarantinopoulos, P., Tsakalidou, E., De Vuyst, L. 2006. The role and application of enterococci in food and health. Int. J. Food Microbiol., vol. 106, no. 1,
p. 1-24. https://doi.org/10.1016/j.ijfoodmicro.2005.06.026 PMid:16216368
Giraffa, G. 2003. Functionality of enterococci in dairy products. Int. J. Food Microbiol., vol. 88, no. 2-3, p. 215-222.
Hamadi, F., Hassan, L., Asserne, F., Elabed, S., Zahir, H., Saad, I. K., Hanine, H., Bengourram, J. 2013. Quantitative adhesion of Staphylococcus aureus on stainless steel coated with milk. Food and Nutrition Sciences, vol. 4, p. 299-304. https://doi.org/10.4236/fns.2013.43040
Jahan, M., Holley, R. A. 2014. Incidence of virulence factors in enterococci from raw and fermented meat and biofilm forming capacity at 25 °C and 37 °C. International Journal of Food Microbiology, vol. 170, p. 65-69. https://doi.org/10.1016/j.ijfoodmicro.2013.11.002 PMid:24291183
Klein, G. 2003. Taxonomy, ecology and antibiotic resistance of enterococci from food and the gastro-intestinal tract. International Journal of Food Microbiology, vol. 88, p. 123-131. https://doi.org/10.1016/S0168-1605(03)00175-2
Koluman, A., Akan, L. S., Cakiroglu, F. P. 2009. Occurence and antimicrobial resistance of enterococci in retail foods. Food Control, vol. 20, p. 281-283. https://doi.org/10.1016/j.foodcont.2008.05.007
Krebs-Artimová, A., Kročko, M., Ducková, V., Čanigová, M. 2010. Testing of sanitary detergents effectiveness to enteroccoci. Potravinárstvo, vol. 4, no. 1, p. 35-38.
Kročko, M., Čanigová, M., Ducková, V., Krebs-Artimová, A., Bezeková, J., Poston, J. 2011. Antibiotic resistance of Enterococcus species isolated from raw foods of animal origin in South West part of Slovakia. Czech Journal of Food Science, vol. 29, no. 6, p. 654-659. [cit. 2015-01-20] Available at: http://www.agriculturejournals.cz/publicFiles/51707.pdf
Lavová, M., Ducková, V., Čanigová, M., Kročko, M. 2011. Enterococci and their ability live out activity of sanitation detergents. Potravinarstvo, vol. 5, no. 4, p. 42-44. https://doi.org/10.5219/166
Mah, T. H. C., O´Toole, G. A. 2001. Mechanisms of biofilm resistance to antimicrobial agents. TRENDS in Microbiology, vol. 9, no. 1, p. 34-39. https://doi.org/10.1016/S0966-842X(00)01913-2
Marinho, A. R., Martins, P. D., Ditmer, E. M., d´ Azevedo, P. A., Frazzon, J., Van Der Sand, S. T., Frazzon, A. P. G. 2013. Biofilm formation on polystyrene under different temperatures by antibiotic resistant Enterococcus faecalis and Enterococcus faecium isolated from food. Brazilian Journal of Microbiology, vol. 44, no. 2, p. 423-426. https://doi.org/10.1590/S1517-83822013005000045 PMid:24294231
Marques, S. C., Rezende, J. G. O. S., Alves, L. A. F., Silva, B. C., Alves, E., de Abreu, L. R., Piccoli, R. H. 2007. Formation of biofilms by Staphylococcus aureus on stainless steel and glass surfaces and its resistance to some selected chemical sanitizers. Brazilian Journal of Microbiology, vol. 38, no. 3, p. 538-543. https://doi.org/10.1590/S1517-83822007000300029
Medeiros, A. W., Pereira, R. I., Martins, P. D., d´ Azevedo, P. A., Van Der Sand, S., Frazzon, J., Frazzon, A. P. G. 2014. Molecular detection of virulence factors among food and clinical Enterococcus faecalis strains in South Brazil. Brazilian Journal of Microbiology, vol. 45, no. 1, p. 327-332. https://doi.org/10.1590/S1517-83822014005000031 PMid:24948952
Necidová, L., Janštová, B., Karpíšková, S., Cupáková, Š., Dušková, M., Karpíšková, R. 2009. Importance of Enterococcus spp. for forming a biofilm. Czech Journal of Food Science, vol. 27, p. S354-S356.
[cit. 2015-01-16] Available at: http://www.agriculturejournals.cz/publicFiles/07943.pdf
Ouali, F. A., Kassaa, I. A., Cudennec, B., Abdallah, M., Bendali, F., Sadoun, D., Chihib, N., Drider, D. 2014. Identification of lactobacilli with inhibitory effect on biofilm formation by pathogenic bacteria on stainless steel surfaces. International Journal of Food Microbiology, vol. 191, p. 116-124. https://doi.org/10.1016/j.ijfoodmicro.2014.09.011 PMid:25261829
Salo, S., Ehavald, H., Raaska, L., Vokk, R., Wirtanen, G. 2006. Microbial surveys in Estonian dairies. LWT, vol. 39, p. 460-471. https://doi.org/10.1016/j.lwt.2005.03.008
Settanni, L., Moschetti, G. 2010. Non-starter lactic acid bacteria used to improve cheese quality and provide health benefits. Food Microbiology, vol. 27, no. 6, p. 691-697. https://doi.org/10.1016/j.fm.2010.05.023 PMid:20630311
Srey, S., Kabir Jahid, K., Ha, S. D. 2013. Biofilm formation in food industries: A food safety concern. Food Control, vol. 31, no. 2, p. 572-585. https://doi.org/10.1016/j.foodcont.2012.12.001
Trachoo, N., Frank, J. F. 2002. Effectiveness of chemical sanitizers against Campylobacter jejuni containing biofilms. Journal of Food Protection, vol. 65, no. 7, p. 1117-1121. PMid:12117244
Tsikrikonis, G., Manaitis, A. N., Labrou, M., Ntokou, E., Michail, G., Daponte, A., Stathopoulos, S., Tsakris, A., Pournaras, S. 2012. Differences in biofilm formation and virulence factors between clinical and faecal enterococcal isolates of human and animal origin. Microbial Pathogenesis, vol. 52, no. 6, p. 336-343. https://doi.org/10.1016/j.micpath.2012.03.003 PMid:22445820
Valenzuela, A. S., Omar, N. B., Abriouel, H., López, R. L., Veljovic, K., Cañamero, M. M., Topisirovic, M. K. L., Gálvez, A. 2009. Virulence factors, antibiotic resistance, and bacteriocins in enterococci from artisan foods of animal origin. Food Control, vol. 20, p. 381-385. https://doi.org/10.1016/j.foodcont.2008.06.004
Wirtanen, G., Salo, S. 2004. DairyNET – hygiene control in dairies. VTT publication 545. Espoo: Otamedia Oy, ISBN 9513864081.
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