The influence of virus infections on antioxidant levels in the genetically modified plum variety "Honeysweet" (Prunus domestica L.)

Authors

  • Jiri Sochor Mendel University in Brno, Faculty of Horticulturae, Department of Viticulture and Enology, Valtická 337, 691 44 Lednice
  • Boris Krska Mendel University in Brno, Faculty of Horticulturae, Department of Fruit Growing, Valtická 337, 691 44 Lednice
  • Jaroslav Polak Crop Research Institute, Division of Crop Protection and Plant Health, Drnovská 406, Praque-Ruzyně
  • Tunde Jurikova Constantine the Philosopher University in Nitra, Faculty of Central European Studies, Institut for teacher training, Drazovska 4, 949 74 Nitra

DOI:

https://doi.org/10.5219/420

Keywords:

Prunus domestica L., viral infection, antioxidants

Abstract

It is well-known that polyphenolic compounds are found abundantly in fruit, but various kinds of diseases  lower these levels. This work measures total polyphenolic content, antioxidant activity and the levels of specific important antioxidants in fruits of the genetically modified (GM) plum variety HoneySweet, trees  which were previously inoculated with a range of different virus infections.  These were the Plum Pox virus (PPV), Prune Dwarf virus (PDV) and Apple Chlorotic Leaf-Spot virus (ACLSV). Uninoculated trees were used as controls. Antioxidant activity was measured using four different photometric  methods – DPPH (2,2-diphenyl-1-picrylhydrazyl), DMPD (N-dimethyl-p-phenylenediamine), ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) and FRAP (Ferric reducing antioxidant power). Total polyphenol content was measured using the Folin–Ciocalteau method. The profiles of 10 specific antioxidant constituents in the fruits of the GM plum variety HoneySweet were detected and analyzed, since these are of interest for their role in human diets and could play a role in the resistance of plants to viruses. Detection was made using HPLC with UV-VIS detection.  They were: gallic acid, p-coumaric acid, 4-aminobenzoic acid, chlorogenic acid, caffeic acid, ferulic acid, vanillin, rutin and quercetin. The compound with the highest concentration was chlorogenic acid (587 mg/100 g), and that with the lowest was p-coumaric acid (0.95 mg/100 g). Of the four methods of antioxidant activity used, in three the lowest levels of antioxidant activity were seen where the PPV virus was combined with ACLSV, and in three the highest levels were seen in the un-inoculated control without any infection. The highest values of total polyphenols were seen in the control (65.3 mg/100 g), followed by infection of PPV, then treatment PPV, PDV and ACLSV, then treatment PPV and PDV and finally the lowest levels were seen in treatment PPV and ACLSV (44.2 mg/100 g), which was also that with the lowest antioxidant activity.

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References

Capote, N., Perez-Panades, J., Monzo, C., Carbonell, E., Urbaneja, A., Scorza, R., Ravelonandro, M., Cambra, M. 2008. Assessment of the diversity and dynamics of plum pox virus and aphid populations in transgenic European plums under mediterranean conditions. Transgenic Research, vol. 17, no. 3, p. 367-377. https://doi.org/10.1007/s11248-007-9112-0 PMid:17605085 DOI: https://doi.org/10.1007/s11248-007-9112-0

Forcada, C. F. I., Gogorcena, Y., Moreno, M. A. 2014. Agronomical parameters, sugar profile and antioxidant compounds of "catherine" peach cultivar influenced by different plum rootstocks. International Journal of Molecular Sciences, vol. 15, no. 2, p. 2237-2254. https://doi.org/10.3390/ijms15022237 DOI: https://doi.org/10.3390/ijms15022237

Gao, H., Cheng, N., Zhou, J., Wang, B. N., Deng, J. J., Cao, W. 2014. Antioxidant activities and phenolic compounds of date plum persimmon (diospyros lotus l.) fruits. J. Food Sci. Technol., vol. 51, no. 5, p. 950-956. https://doi.org/10.1007/s13197-011-0591-x PMid:24803703 DOI: https://doi.org/10.1007/s13197-011-0591-x

Jaiswal, R., Karakose, H., Ruhmann, S., Goldner, K., Neumuller, M., Treutter, D., Kuhnert, N. 2013. Identification of phenolic compounds in plum fruits (Prunus salicina l. And prunus domestica l.) by high-performance liquid chromatography/tandem mass spectrometry and characterization of varieties by quantitative phenolic fingerprints. Journal of Agricultural and Food Chemistry, vol. 61, p. 12020-12031. https://doi.org/10.1021/jf402288j PMid:24152059 DOI: https://doi.org/10.1021/jf402288j

Kaulmann, A., Jonville, M. C., Schneider, Y. J., Hoffmann, L., Bohn, T. 2014. Carotenoids, polyphenols and micronutrient profiles of brassica oleraceae and plum varieties and their contribution to measures of total antioxidant capacity. Food chemistry, vol. 155, p. 240-250. https://doi.org/10.1016/j.foodchem.2014.01.070 PMid:24594181 DOI: https://doi.org/10.1016/j.foodchem.2014.01.070

Los, J., Wilska-Jeszka, J., Pawlak, M. 2000. Polyphenolic compounds of plums (Prunus domestica). Polish Journal of Food and Nutrition Sciences, vol. 9, p. 35-38. ISSN: 1230-0322.

Miletic, N., Popovic, B., Mitrovic, O., Kandic, M., Leposavic, A. 2014. Phenolic compounds and antioxidant capacity of dried and candied fruits commonly consumed in serbia. Czech Journal of Food Sciences, vol. 32, no. 4, p. 360-368. [cit.2014-12-12] Available at: http://www.agriculturejournals.cz/publicFiles/128186.pdf DOI: https://doi.org/10.17221/166/2013-CJFS

Morabbi Najafabad, A., Jamei, R. 2014. Free radical scavenging capacity and antioxidant activity of methanolic and ethanolic extracts of plum (prunus domestica l.) in both fresh and dried samples. Avicenna journal of phytomedicine, vol. 4, p. 343-53. ISSN: 2228-7930.

Pohanka, M., Sochor, J., Ruttkay-Nedecky, B., Cernei, N., Adam, V., Hubalek, J., Stiborova, M., Eckschlager, T., Kizek, R. 2012. Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry. Journal of Applied Biomedicine, vol. 10, no. 3, p. 155-167. https://doi.org/10.2478/v10136-012-0006-y DOI: https://doi.org/10.2478/v10136-012-0006-y

Polak, J., Kumar, J., Krska, B., Ravelonandro, M. 2012. Biotech/gm crops in horticulture: Plum cv. Honeysweet resistant to plum pox virus. Plant Protection Science, vol. 48, p. S43-S48. ISSN: 1212-2580. [cit.2014-12-12] Available at: http://www.agriculturejournals.cz/publicFiles/80331.pdf DOI: https://doi.org/10.17221/37/2012-PPS

Popov, S. V., Ovodova, R. G., Golovchenko, V. V., Khramova, D. S., Markov, P. A., Smirnov, V. V., Shashkov, A. S., Ovodov, Y. S. 2014. Pectic polysaccharides of the fresh plum Prunus domestica l. Isolated with a simulated gastric fluid and their anti-inflammatory and antioxidant activities. Food chemistry, vol. 143, p. 106-113. https://doi.org/10.1016/j.foodchem.2013.07.049 DOI: https://doi.org/10.1016/j.foodchem.2013.07.049

Ravelonandro, M., Scorza, R., Polak, J., Callahan, A., Krska, B., Kundu, J., Briard, P. 2013. "Honeysweet" plum–a valuable genetically engineered fruit-tree cultivar. Food and Nutrition Sciences, vol. 4, p. 45-49. https://doi.org/10.4236/fns.2013.46A005 DOI: https://doi.org/10.4236/fns.2013.46A005

Rop, O., Jurikova, T., Mlcek, J., Kramarova, D., Sengee, Z. 2009. Antioxidant activity and selected nutritional values of plums (prunus domestica l.) typical of the white carpathian mountains. Scientia Horticulturae, vol. 122, no. 4, p. 545-549. https://doi.org/10.1016/j.scienta.2009.06.036 DOI: https://doi.org/10.1016/j.scienta.2009.06.036

Sanchez-Rangel, J. C., Benavides, J., Heredia, J. B., Cisneros-Zevallos, L., Jacobo-Velazquez, D. A. 2013. The folin-ciocalteu assay revisited: Improvement of its specificity for total phenolic content determination. Analytical Methods, vol. 5, p. 5990-5999. https://doi.org/10.1039/c3ay41125g DOI: https://doi.org/10.1039/c3ay41125g

Sochor, J., Majzlik, P., Salas, P., Adam, V., Trnkova, L., Hubalek, J., Kizek, R. 2010a. Studium dostupnosti iontů těžkých kovů pomocí různých extrakčních postupů a elektrochemické detekce (A study of availability of heavy metal ions by using various exracction procedures and electrochemical detection.) Listy Cukrovarnicke a Reparske, vol. 126, p. 414-415. ISSN: 1210-3306. [cit.2014-12-12] Available at: http://www.cukr-listy.cz/on_line/2010/PDF/414-415.PDF

Sochor, J., Ryvolova, M., Krystofova, O., Salas, P., Hubalek, J., Adam, V., Trnkova, L., Havel, L., Beklova, M., Zehnalek, J., Provaznik, I., Kizek, R. 2010b. Fully automated spectrometric protocols for determination of antioxidant activity: Advantages and disadvantages. Molecules, vol. 15, no. 12, p. 8618-8640. https://doi.org/10.3390/molecules15128618 PMid:21116230 DOI: https://doi.org/10.3390/molecules15128618

Sochor, J., Skutkova, H., Babula, P., Zitka, O., Cernei, N., Rop, O., Krska, B., Adam, V., Provaznik, I., Kizek, R. 2011. Mathematical evaluation of the amino acid and polyphenol content and antioxidant activities of fruits from different apricot cultivars. Molecules, vol. 16, no. 9, p. 7428-7457. https://doi.org/10.3390/molecules16097428 DOI: https://doi.org/10.3390/molecules16097428

Srivastava, M., Hegde, M., Chiruvella, K. K., Koroth, J., Bhattacharya, S., Choudhary, B., Raghavan, S. C. 2014. Sapodilla plum (Achras sapota) induces apoptosis in cancer cell lines and inhibits tumor progression in mice. Scientific Reports, vol. 4. https://doi.org/10.1038/srep06147 DOI: https://doi.org/10.1038/srep06147

Treutter, D., Wang, D., Farag, M. A., Baires, G. D., Ruhmann, S., Neumuller, M. 2012. Diversity of phenolic profiles in the fruit skin of prunus domestica plums and related species. J. Agric. Food Chem., vol. 60, p. 12011-12019. https://doi.org/10.1021/jf303644f PMid:23140499 DOI: https://doi.org/10.1021/jf303644f

Utsunomiya, H., Yamakawa, T., Kamei, J., Kadonosono, K.,Tanaka, S. I. 2005. Anti-hyperglycemic effects of plum in a rat model of obesity and type 2 diabetes, wistar fatty rat. Biomedical Research-Tokyo, vol. 26, no. 5, p. 193-200. https://doi.org/10.2220/biomedres.26.193 PMid:16295695 DOI: https://doi.org/10.2220/biomedres.26.193

Venter, A., Joubert, E., De Beer, D. 2014. Nutraceutical value of yellow- and red-fleshed South african plums (Prunus salicina Lindl.): Evaluation of total antioxidant capacity and phenolic composition. Molecules, vol. 19, no. 3, p. 3084-3109. https://doi.org/10.3390/molecules19033084 PMid:24619353 DOI: https://doi.org/10.3390/molecules19033084

Vizzotto, M., Porter, W., Byrne, D., Cisneros-Zevallos, L. 2014. Polyphenols of selected peach and plum genotypes reduce cell viability and inhibit proliferation of breast cancer cells while not affecting normal cells. Food chemistry, vol. 164, p. 363-370. https://doi.org/10.1016/j.foodchem.2014.05.060 PMid:24996346 DOI: https://doi.org/10.1016/j.foodchem.2014.05.060

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Published

2015-07-07

How to Cite

Sochor, J. ., Krska, B. ., Polak, J. ., & Jurikova, T. . (2015). The influence of virus infections on antioxidant levels in the genetically modified plum variety "Honeysweet" (Prunus domestica L.). Potravinarstvo Slovak Journal of Food Sciences, 9(1), 195–200. https://doi.org/10.5219/420

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