Redistribution of mineral elements in wheat grain when applying the complex enzyme preparations based on phytase
Keywords:phytin, phytase, complex enzyme preparation, microstructure, mineral element, grain of wheat
Biogenic minerals play an important role in the whole human nutrition, but they are included in the grain of the phytates that reduces their bioavailability. Whole wheat bread is generally considered a healthy food, but the presence of mineral elements in it is insignificant, because of weak phytate degradation. From all sources of exogenous phytase the most productive are microscopic fungi. To accelerate the process of transition hard mineral elements are mobilized to implement integrated cellulolytic enzyme preparation based on the actions of phytase (producer is Penicillium canescens). Phytase activity was assessed indirectly by the rate of release of phosphate from the substrate. It has been established that the release rate of the phosphoric acid substrate is dependent on the composition of the drug and the enzyme complex is determined by the presence of xylanase. The presented experimental data shows that a cellulase treatment of the grain in conjunction with the β-glucanase or xylanase leading to an increase in phytase activity could be 1.4 - 2.3 times as compared with the individual enzymes. As a result of concerted action of enzymes complex preparation varies topography grain, increase the pore sizes in seed and fruit shells that facilitate the penetration of the enzyme phytase in the aleurone layer to the site of phytin hydrolysis and leads to an increase in phytase activity. In terms of rational parameters of enzymatic hydrolysis, the distribution of mineral elements in the anatomical parts of the grain after processing complex enzyme preparation with the help of X-ray detector EMF miniCup system in a scanning electron microscope JEOL JSM 6390 were investigated. When processing enzyme preparation wheat trend in the distribution of mineral elements, characteristic of grain - the proportion of these elements in the aleurone layer decreases, and in the endosperm increases. Because dietary fiber and phytate found together in the peripheral layers of fiber-rich grains, it is difficult to separate the effects of degradation processes nonstarch polysaccharides and fiberphytate redistribution of polyvalent metal ions. However, studies have shown that phytase - an effective mechanism for regulating mineral nutrient diet. Application of phytase in grain bakery technology will increase the biological value of the product.
Bergman, C. J., Gualberto, D. G., Weber, C. W. 1997. Mineral binding capacity of dephytinized insoluble fiber from extruded wheat, oat and rice brans. Plant Foods for Human Nutrition. vol. 51, no. 4, p 295-310. https://doi.org/10.1023/A:1007972205452 PMid:9650723
Buddrick, O., Jones, O. A. H., Cornell, H. J., Small, D. M. 2014. The influence of fermentation processes and cereal grains in wholegrain bread on reducing phytate content. Journal of Cereal Science. vol. 59, no. 1, p. 3-8. https://doi.org/10.1016/j.jcs.2013.11.006
Eastwood, D., Laidman, D. L. 1971. The mobilization of macronutrient elements in the germinating wheat grain. Phytochemistry. vol. 10, no. 6, p. 1275-1284. https://doi.org/10.1016/S0031-9422(00)84328-9
Eklund-Jonsson, Ch., Sandberg, A.-S., Alminger, M. L. 2006. Reduction of phytate content while preserving minerals during whole grain cereal tempe fermentation. Journal of Cereal Science. vol. 44, no. 2, p. 154-160. https://doi.org/10.1016/j.jcs.2006.05.005
Erdman, J. W. 1979. Oilseed phytates: Nutritional implications. Journal of the American Oil Chemists Society. vol. 56, no. 8, p. 736-741. https://doi.org/10.1007/BF02663052
García-Estepa, R. M., Guerra-Hernández, E., García-Villanova, B. 1999. Phytic acid content in milled cereal products and breads. Food Research International. vol. 32, no. 3, p. 217-221. https://doi.org/10.1016/S0963-9969(99)00092-7
Graf, E., Empson, K. L., Eaton, J. W. 1987. Phytic acid. A natural antioxidant. Journal of Biological Chemistry. vol. 262, no. 24, p. 11647-11650. PMid:3040709
Greiner, R., Konietzny, U. 2006. Phytase for food application (Review). Food Technology and Biotechnology. vol. 44, no. 2, p. 125-140.
Haefner, S., Knietch, A., Scholten, E., Braun, J., Lohscheidt, M., Zelder, O.s 2005. Biotechnological production and applications of phytases. Short Survey Applied Microbiology and Biotechnology. vol. 68, no. 5, p. 588-597. https://doi.org/10.1007/s00253-005-0005-y PMid:16041577
Haraldsson, A.-K., Rimsten, L., Alminger, M. L., Andersson, R., Andlid, T., Åman, P., Sandberg, A.-S. 2004. Phytate content is reduced and β-glucanase activity suppressed in malted barley steeped with lactic acid at high temperature. Journal of the Science of Food and Agriculture. vol. 84, no. 7, p. 653-662. https://doi.org/10.1002/jsfa.1724
Haros, M., Rosell, C. M., Benedito, C. 2001. Fungal phytase as a potential breadmaking additive. European Food Research and Technology. vol. 213, no. 4-5, p. 317-322. https://doi.org/10.1007/s002170100396
Huan-Joo, H.-J., Kim, J.-H., Kim, M.-J. 2004. Comprasion of irradiated phytic acid and other antioxidations for antioxidant activity. Food Chemistry. vol. 88, no. 2, p. 173-178. https://doi.org/10.1016/j.foodchem.2004.02.001
Cheryan, M. 1980. Phytic acid interactions in food systems. (Review). Critical reviews in food science and nutrition. vol. 13, no. 4, p. 297-335. https://doi.org/10.1080/10408398009527293 PMid:7002470
Kabata-Pendias A., Pendias X. 1989. Agricultural trace elements in soils and plants. Мoskow: Mir, 439 p.
Kuznetsova, E. A., Karachkina, S. Ya., Prigarina, O. M, Sinitsyn, A. P. 2006. The influence of the enzyme preparation on the basis of phytase in the complex with amber acid on the quality of the grain bread. Bread Products. (Мoskow), no. 9, p. 62-64.
Kuznetsova, E. A., Cherepnina, L. V., Klepov, R. E., Sinitsyn, A. P., Sinitsyna, O. A. 2012. Preparation influence on a basis phytase on change carbohydrate amylase and lipid complexes of grain raw materials. Bread Products (Мoskow), no. 10, p. 50-52.
Lestienne, I., Isard-Verniere, Ch., Mounquet, C., Picq, C., Treche, S. 2005. Effects of soaking whole cereal and lequme seeds in iron, zinc and phytate contents. Food Chemistry. vol. 89, no. 3. p. 421-425. https://doi.org/10.1016/j.foodchem.2004.03.040
Lonnerdal, B. 2002. Phytic acid-trace element (Zn, Cu, Mn) interactions. Journal of Food Science and Technology. vol. 37, no. 7, p. 749-758. https://doi.org/10.1046/j.1365-2621.2002.00640.x
Lott, J. N. A., Ockenden, I., Raboy, V., Batten, G. D. 2000. Phytic acid and phosphorus in crop seeds and fruits: A global estimate (Review). Seed Science Research. vol. 10, no. 1, p. 11-33.
Madrid, J., Martínez, S., López, C., Hernández, F. 2013. Effect of phytase on nutrient digestibility, mineral utilization and performance in growing pigs. Livestock Science. vol. 154, no. 1-3, p. 144-151. https://doi.org/10.1016/j.livsci.2013.03.003
McKenzie-Parnell, J. M., Davies N. T. 1986. Destruction of phytic acid during home breadmaking. Food Chemistry. vol. 22, no. 3, p. 181-192. https://doi.org/10.1016/0308-8146(86)90077-4
Nielsen, M. M., Damstrup, M. L., Dal Thomsen, A., Rasmussen, S. K., Hansen, Å. 2007. Phytase activity and degradation of phytic acid during rye bread making. European Food Research and Technology. vol. 225, no. 2, p. 173-181. https://doi.org/10.1007/s00217-006-0397-7
Sinitsyna, O. A., Bukhtoyarov, F. E., Gusakov, A. V., Sinitcyn, A. P., Okunev, O. N., Bekkarevitch, A. O., Vinetsky, Yu. P. 2003. Isolation and properties of major components of Penicillium canescens extracellular enzyme complex. Biochemistry (Moscow), vol. 68, no. 11, p. 1200-1209. https://doi.org/10.1023/B:BIRY.0000009134.48246.7e
Tanaka, K., Yoshida, T., Kasai, Z. 1974. Distribution of mineral elements in the outer layer of rice and wheat grains, using electron microprobe X-Ray analysis. Soil Science and Plant Nutrition. vol. 20, no. 1, p. 87-91. https://doi.org/10.1080/00380768.1974.10433231
Torre, M., Rodriguez, A. R., Saura-Calixto, F. 1991. Effects of dietary fiber and phytic acid on mineral availability. (Review). Critical reviews in food science and nutrition. vol. 30, no 1, p. 1-22. https://doi.org/10.1080/10408399109527539 PMid:1657026
Türk, M., Carlsson, N.-G., Sandberg, A.-S. 1996. Reduction in the levels of phytate during wholemeal bread making; effect of yeast and wheat phytases. Journal of Cereal Science. vol. 23, no. 3, p. 257-264. https://doi.org/10.1006/jcrs.1996.0026
Urbano, G., Lopez-Jurado, M., Aranda, P., Vidal-Valerde, C., Tenorio, E., Porres, J. 2000. The role of phytic acid in legumes: Antinutrient or beneficial function? Journal of Physiology and Biochemistry. vol. 56, no 3, p. 283-294. https://doi.org/10.1007/BF03179796 PMid:11198165
Wodzinski, R. J., Ullah, A. H. J. 1996. Phytase (Review). Advances in Applied Microbiology. vol. 42, p. 263-302. https://doi.org/10.1016/S0065-2164(08)70375-7
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