Total polyphenol content and antioxidant capacity changes in dependence on chosen garden pea varieties

Authors

  • Alžbeta Hegedüsová Slovak University of Agriculture in Nitra, Horticulture and Landscape Engineering Faculty, Department of Vegetable Production, Tr. A. Hlinku 2, 949 76 Nitra
  • Ivana Mezeyová Slovak University of Agriculture in Nitra, Horticulture and Landscape Engineering Faculty, Department of Vegetable Production, Tr. A. Hlinku 2, 949 76 Nitra
  • Mária Timoracká Slovak University of Agriculture in Nitra, Horticulture and Landscape Engineering Faculty, Department of Vegetable Production, Tr. A. Hlinku 2, 949 76 Nitra
  • Miroslav Šlosár Slovak University of Agriculture in Nitra, Horticulture and Landscape Engineering Faculty, Department of Vegetable Production, Tr. A. Hlinku 2, 949 76 Nitra
  • Janette Musilová Slovak University of Agriculture in Nitra, Horticulture and Landscape Engineering Faculty, Department of Vegetable Production, Tr. A. Hlinku 2, 949 76 Nitra
  • Tünde Jurí­ková Constantine the Philosopher University in Nitra, Institute for Teacher Training, Faculty of Central European Studies, Drazovska 4, Nitra

DOI:

https://doi.org/10.5219/412

Keywords:

garden pea, total polyphenols, antioxidant capacity

Abstract

The green pea is ranged between the crops with high antioxidant potential. This potential is connected with phytochemical components mainly with polyphenols. All these bioactive chemicals have disease - fighting properties. In real human diet there is no usually possibility of fresh garden pea consumption during the whole year. The total polyphenol content is significantly changed among other things by processing methods. Focus on variety, bio-fortification and other specific agricultural inputs, could be the right method of total polyphenol contents and total antioxidant capacity increasing. The main objective of the present work was to consider the changes of total polyphenols content in dependence on variety and to evaluate an antioxidant potential six garden pea varieties arranged by the ripening point of view. Variety “Exzeleus” belongs to very early type, ́Premium ́ is early maturing,”Flavora” is middle ripening variety and the last three varieties “Utrio”, “Jumbo” and “Ambassador” are middle late types of varieties. Every variety was grown in four replications, i.e. on 24 m2 total plot in Botanical garden of Slovak University of Agriculture in Nitra during 2013. Total polyphenols were determined by the Lachmans method and expressed as mg of gallic acid equivalent per kg fresh mater. Total antioxidant capacity was measured by the Brand - Williams method using a compound DPPH (2.2-diphenyl-1-pikrylhydrazyl)). The highest value was reached in case of variety “Jumbo” 1179.995±28.081 mg/kg, the lowest value in case of “Premium” 674.505 ±26.541 mg/kg. When evaluating an antioxidant capacity in chosen varieties of garden pea, the interval estimated by our trail ranged from 0.523 ±0.206% (“Exzeleus”) to 6.844 ±0.591% (“Flavora”) Following the both observed parameters, TPC and TAC, variety “Flavora” (as a member of middle ripening varieties) seems to be the most optimal from the human nutrition point of view. The various varieties had significant influence on TPC and TAC according to used statistical analyzes. Within the all observed varieties, when they were arranged by ripening, there was estimated significant difference only in case of garden pea varieties early – middle late. Other couples didn’t show any statistical important differences in total polyphenol content.

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References

Amarakoon, D., Thavarajah, D., Gupta, D., Mcphee, K., Desutter, T., Thavarajah, P. 2014. Genetic and environmental variation of seed iron and food matrix factors of North-Dakota-grown field peas (Pisum sativum L.). J. Food Compos. Anal., vol. 37, p. 67-74. https://doi.org/10.1016/j.jfca.2014.09.001 DOI: https://doi.org/10.1016/j.jfca.2014.09.001

Brand-Williams, W., Cuvelier, M. E., Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft and Technologie, vol. 28, no. 1, p. 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5 DOI: https://doi.org/10.1016/S0023-6438(95)80008-5

Broadley, M. R., Alcock, J., Alford, J., Cartwright, P., Foot, I., Fairweather-Tait, S. J., Zhao, F. J. (2010). Selenium biofortification of high-yielding winter wheat (Triticum aestivum L.) by liquid or granular Se fertilization. Plant and Soil, vol. 332, p. 5-18. https://doi.org/10.1007/s11104-009-0234-4 DOI: https://doi.org/10.1007/s11104-009-0234-4

D’archivio, M., Filesi, C., Benedetto, R., Gargiulo, R., Giovannini, C., Masella R. 2007 Polyphenols, dietary sources and bioavailability, Ann Ist Super Sanità., vol. 43, no. 4., p. 348-361. [cit. 2014-12-10] Retrieved from the web: http://www.iss.it/publ/anna/2007/4/434348.pdf

Elbling, L, Weiss, R. M., Teufelhofer, O., Uhl, M., Knasmueller, S., Schulte-Hermann, R., Berger, W., Micksche, M. 2005. Green tea extract and (-)-epigallocatechin-3-gallate, the major tea catechin, exert oxidant but lack antioxidant activities, The FASEB Journal, vol. 19, no. 7, p. 807-809. https://doi.org/10.1096/fj.04-2915fje PMid:15738004 DOI: https://doi.org/10.1096/fj.04-2915fje

Fratianni, F., Cardinalea, F., Cozzolinoa, A, Granesea, T. 2014. Polyphenol composition and antioxidant activity of different grass pea (Lathyrus sativus), lentils (Lens culinaris), and chickpea (Cicer arietinum) ecotypes of the Campania region (Southern Italy), Journal of functional foods, vol. 7, p. 551-557. https://doi.org/10.1016/j.jff.2013.12.030 DOI: https://doi.org/10.1016/j.jff.2013.12.030

Kavalcová, P., Bystrická, J., Tomáš, J., Karovičová, J., Kuchtová, V., 2014. Evaluation and comparing of the content of total polyphenols and antioxidant activity in onion, garlic and leek, Potravinarstvo, vol. 8, no. 1, p. 272-276. https://doi.org/10.5219/394 DOI: https://doi.org/10.5219/394

Lachman, J., Proněk, D., Hejtmánková, A., Pivec, V., Faitová, K. 2003. Total polyphenol and main flavonoid antioxidants in different onion (Allium cepa L.) varieties. Scientia Horticulturae, vol. 30, p. 142-147. DOI: https://doi.org/10.17221/3876-HORTSCI

[cit. 2014-05-21] Retrieved from the web: http://www.agriculturejournals.cz/publicFiles/51932.pdf

Lambert, J. D., Hong, J., Yang, G. Y., Liao, J., Yang, C. S. 2005. Inhibition of carcinogenesis by polyphenols: evidence from laboratory investigations. Am. J. Clin. Nutr., vol. 81, no. 1, p. 284S-291S., on-line ISSN: 1938-3207, Print ISSN: 0002-9165 PMid:15640492 DOI: https://doi.org/10.1093/ajcn/81.1.284S

Lee, C. Y. 2013. Challenges in providing credible scientific evidence of health benefits of dietary polyphenols. Journal of Functional Foods, vol. 5, p. 524-526. https://doi.org/10.1016/j.jff.2012.10.018 DOI: https://doi.org/10.1016/j.jff.2012.10.018

Poblaciones, M., Rodrigo, S. M., Santamaría, O. 2013. Evaluation of the Potential of Peas (Pisum sativum L.) to Be Used in Selenium biofortification Programs Under Mediterranean Conditions, Biol Trace Elem Res, https://doi.org/10.1007/s12011-012-9539-x PMid:23129527 DOI: https://doi.org/10.1007/s12011-012-9539-x

Mushtaq, M., Wani, S. M. 2013. Polyphenols and human health - a review, International Journal of Pharma and Bio Sciences, vol. 4(2), p. 338-360, [cit. 2014-12-04] Available at: http://worldcocoafoundation.org/wp-content/files_mf/mushtaq2013humannutritionpolyphenolreview.pdf

Nilsson, J., Stegmark, R., Akesson, B. 2004. Total antioxidant capacity in different pea (Pisum sativum) varieties after blanching and freezing, Food Chemistry, vol. 86, no. 4, p. 501-507. https://doi.org/10.1016/j.foodchem.2003.09.002 DOI: https://doi.org/10.1016/j.foodchem.2003.09.002

Nithiyanantham, S., Selvakumar, S., Siddhuraju S. 2012. Total phenolic content and antioxidant activity of two different solvent extracts from raw and processed legumes, Cicer arietinum L. and Pisum sativum L. Journal of food Composition and Analysis, vol. 27, no. 1, p. 52-60. https://doi.org/10.1016/j.jfca.2012.04.003 DOI: https://doi.org/10.1016/j.jfca.2012.04.003

Pandey, K. B., Rizvi, S. I. 2009. Plant polyphenols as dietary antioxidants in human health and disease. Oxidative Medicine and Cellular Longevity, vol. 2, no. 5, p. 270-278. https://doi.org/10.4161/oxim.2.5.9498 DOI: https://doi.org/10.4161/oxim.2.5.9498

Petchiammal, C., Waheeta H. 2014. Antioxidant activity of proteins from fifteen varieties of legume seeds commonly consumed in India. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, p. 476-479.

[cit. 2014-11-12] Available at: http://www.ijppsjournal.com/Vol6Suppl2/8471.pdf

Seo, O. N., Kim, G. S., Park, S., Lee, J. H., Kim, Y. H., Lee, W. S., Lee, S. J., Kim, C. Y., Jin, J. S., Choi, S. G., Shin, S. C. 2012. Determination of polyphenol components of Lonicera japonica Thunb. using liquid

chromatography – tandem mass spectrometry: Contribution to the overall antioxidant activity. Food Chemistry, vol. 134, no. 1, p. 572-577. https://doi.org/10.1016/j.foodchem.2012.02.124 DOI: https://doi.org/10.1016/j.foodchem.2012.02.124

Singh, J., Basu, P. S. 2012. Non-Nutritive Bioactive Compounds in Pulses and Their Impact on Human Health: An Overview, Food and Nutrition Sciences, vol. 3, p. 1664-1672. https://doi.org/10.4236/fns.2012.312218 DOI: https://doi.org/10.4236/fns.2012.312218

Talukdar, D. 2013. In Vitro Antioxidant Potential and Type II Diabetes Related Enzyme Inhibition Properties of Traditionally Processed Legume-based Food and Medicinal Recipes in Indian Himalayas. Journal of Applied Pharmaceutical Science, vol. 3, no. 1, p. 26-32. https://doi.org/10.7324/JAPS.2013.30106 DOI: https://doi.org/10.7324/JAPS.2013.30106

Troszynska, A., Estrella. I., Lopez-Amores, M. L., Hernandez T. 2002. Antioxidant Activity of Pea (Pisum sativum L.) Seed Coat Acetone Extract. LWT - Food Science and Technology, vol. 35, no. 2, p. 158-164. https://doi.org/10.1006/fstl.2001.0831 DOI: https://doi.org/10.1006/fstl.2001.0831

Turkmenn, N., Sari, F., Velioglu, Y. S., 2005. The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables, Food Chemistry, vol. 93, no. 4, p. 713-718. https://doi.org/10.1016/j.foodchem.2004.12.038 DOI: https://doi.org/10.1016/j.foodchem.2004.12.038

Xu, B. S., Chang, K. C., 2008. Effect of Soaking, Boiling, and Steaming on Total Phenolic Content and Antioxidant Activities of Cool Season Food Legumes, Food Chemistry, vol. 110, no. 1, p. 1-13. https://doi.org/10.1016/j.foodchem.2008.01.045 DOI: https://doi.org/10.1016/j.foodchem.2008.01.045

Halvorsen, B. L., Holte, K., Myhrstad, M. C. W., Barikmoi, I. 2002. A systematic screening of total antioxidants in dietary plants. Journal of Nutrition, vol. 132, p. 461-471, [cit. 2014-12-10] Retrieved from the web: http://jn.nutrition.org/content/132/3/461.full.pdf+html DOI: https://doi.org/10.1093/jn/132.3.461

Timoracka, M., Vollmannova, A. 2010. Determination of flavonoids content in colored peas (Pisum sativum L.) in relation to cultivar’s dependence and storage duration under natural conditions, Potravinarstvo, vol. 4, no. 3, p. 58-62. https://doi.org/10.5219/70 DOI: https://doi.org/10.5219/70

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Published

2015-03-06

How to Cite

Hegedüsová, A. ., Mezeyová, I. ., Timoracká, M. ., Šlosár, M. ., Musilová, J. ., & Jurí­ková, T. . (2015). Total polyphenol content and antioxidant capacity changes in dependence on chosen garden pea varieties. Potravinarstvo Slovak Journal of Food Sciences, 9(1), 1–8. https://doi.org/10.5219/412

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