Detection and characterisation of Plum pox virus (PPV) isolates from Eastern Slovakia revealed the presence of three main viral strains.


  • Július Rozák Central Controlling and Testing Institute in Agriculture, Department of Diagnostic, Osada 281, 044 57 Haniská pri Košiciach
  • Lukáš Predajňa Institute of Virology, Department of Plant Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 84505 Bratislava
  • Zdenka Gálová Slovak University of Agriculture in Nitra, Department of Biochemistry and Biotechnology, Faculty of Biotechnology and Food Sciences,Trieda A. Hlinku 2, 949 76 Nitra
  • Miroslav Glasa Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava



Prunus, virus, RT-PCR, strain, diversity


Plum pox virus (PPV), the agent responsible for Sharka disease, is the most important viral pathogen of stone fruit trees world-wide, having an endemic status in Slovakia. To increase knowledge of PPV diversity in Slovakia, a set of 11 isolates, originated from the eastern part of the country, was characterised. The isolates were chip-budded from their original Prunus hosts to the susceptible GF305 indicators, exhibiting the symptoms of variable severity. A genomic region encompassing the partial NIb and the hypervariable 5´terminal region of the CP gene was amplified from all 11 isolates in RT-PCR and directly sequenced. The phylogenetic analysis revealed the grouping of the 11 Slovak isolates into 3 distinct clusters, representing the PPV-M (2 isolates), D (7 isolates) and Rec strains (2 isolates). The strain affiliation of isolates was further confirmed by strain-specific RT-PCR, using which the presence of additional mixed infection by minor PPV variants was detected in 2 samples. The results further contribute to the understanding of PPV diversity in Slovakia and confirm the specificity and sensitivity of molecular approaches used for the virus strain determination.


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Cambra, M., Capote, N., Myrta, A., Llacer, G. 2006. Plum pox virus and the estimated costs associated with Sharka disease. EPPO Bulletin. vol. 36, no. 2, p. 202-204. DOI:

Candresse, T., Cambra, M., Dallot, S., Lanneau, M., Asensio, M., Gorris, M. T., Revers, F., Macquaire, G., Olmos, A., Boscia, D., Quiot, J. B. Dunez, J. 1998. Comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the D and M serotypes of plum pox potyvirus. Phytopathology. vol. 88, no. 3, p. 198-204. PMid: 18944965 DOI:

Desbiez, C., Moury, B., Lecoq, H. 2011. The hallmarks of "green" viruses: Do plant viruses evolve differently from the others? Infection, Genet. and Evol. vol. 11, no. 5, p. 812-824. PMid: 21382520 DOI:

Garcia, J. A., Glasa, M., Cambra, M., Candresse, T. 2013. Plum pox virus and sharka: a model potyvirus and a major disease. Molecular Plant Pathology, in press, PMid: 24102673 DOI:

García, J. A. Cambra, M. 2007. Plum pox virus and sharka disease. Plant Viruses vol. 1, p. 69-79.

Glasa, M., Marie-Jeanne, V., Labonne, G., Šubr, Z., Kúdela, O., Quiot, J. B. 2002. A natural population of recombinant Plum pox virus is viable and competitive under field conditions. Eur. J. Plant Pathol. vol. 108, p. 843-853. DOI:

Glasa, M., Matisová, J., Kúdela, O. 1998. Characterization of Plum pox virus isolates from Slovakia. Acta Virol. Vol. 42, no. 4, p. 226-229. PMid: 10073223

Glasa M., Predajňa, L., Šubr, Z. W. 2010. Competitiveness of different plum pox virus isolates in experimental mixed infection reveals rather isolate- than strain-specific behaviour. J. Plant Pathol. vol. 92, p. 267-271.

Glasa, M., Candresse, T. 2008. Plum Pox Virus. Encyclopedia of Virology, Third Edition, vol. 4, p. 238-242. DOI:

Glasa, M., Malinowski, T., Predajňa, L., Pupola, N., Dekena, D., Michalczuk, L., Candresse, T. 2011. Sequence variability, recombination analysis, and specific detection of the W strain of Plum pox virus. Phytopathology, vol. 101, no. 8, p. 980-985. PMid: 21425932 DOI:

Glasa, M., Matisová, J., Hričovský, I., Kúdela, O. 1997. Susceptibility of peach GF 305 seedlings and selected herbaceous plants to Plum pox virus isolates from western Slovakia. Acta Virol. vol. 41, p. 341-344. PMid: 9607094

Glasa, M., Palkovics, L., Komínek, P., Labonne, G., Pittnerová, S., Kúdela, O., Candresse, T., Šubr, Z. 2004. Geographically and temporally distant natural recombinant isolates of Plum pox virus (PPV) are genetically very similar and form a unique PPV subgroup. J. Gen. Virol. vol. 85, no. 9, p. 2671-2681. PMid: 15302961 DOI:

Glasa, M., Prikhodko, Y., Predajna, L., Nagyova, A., Shneyder, Y., Zhivaeva, T., Subr, Z., Cambra, M., Candresse, T. 2013. Characterization of sour cherry isolates of Plum pox virus from the Volga basin in Russia reveals a new cherry strain of the virus. Phytopathology. vol. 103, no. 9, p. 972-979. PMid: 23581702 DOI:

Jarošová, J., Polák, J., Kumar, J 2008. Molecular detection of stone fruits virus methodology by RT-PCR and multiplex - RT-PCR (in Czech). Praha: Výzkumný ústav rostlinné výroby, v.v.i., p. 26. ISBN 978-80-87011-86-7.

Jridi, C., Martin, J. F., Marie-Jeanne, V., Labonne, G., Blanc, S. 2006. Distinct viral populations differentiate and evolve independently in a single perennial host plant. J. Virol. vol. 80, no. 5, p. 2349-2357. JVI.80.5.2349-2357.2006 PMid: 16474141 DOI:

Králiková, K. 1964. Survey on the investigation of plum pox disease in Slovakia. Plant Virology: 5th Conference of Czechoslovak Plant Virologists, p. 346-351.

Labonne, G., Yvon, M., Quiot, J. B., Avinent, L., Llacer, G. 1995. Aphids as potential vectors of Plum pox virus: comparison of methods of testing and epidemiological consequensec. Acta Hortic. vol. 386, p. 207-218. DOI:

López-Moya, J. J., Fernandez-Fernandez, M. R., Cambra, M., García, J.A. 2000. Biotechnological aspects of Plum pox virus. Journal of Biotechnology. vol. 76, no. 2-3, p. 121-136. DOI:

Mavrodieva, V., James, D., Williams, K., Negi, S., Varga, A., Mock, R., Levy, L. 2013. Molecular analysis of a Plum pox virus W isolate in plum germplasm hand carried into the USA from the Ukraine shows a close relationship to a Latvian isolate. Plant Disease. vol. 97, no. 1, p. 44-52. DOI:

Paulechová, K. 1981. Comparison of plum pox virus isolates from Czechoslovakia. Biologia vol. 36, p. 225-229.

Predajňa, L. 2013. Molekulárna diverzita a epidemiológia vírusu šarky slivky (PPV). Disertation theses, PriFUK Bratislava, p. 140.

Predajňa, L., Šubr, Z., Candresse, T., Glasa, M. 2012. Evaluation of the genetic diversity of Plum pox virus in a single plum tree. Virus Research. vol. 167, no. 1, p. 112-117. PMid: 22521553 DOI:

Šubr, Z., Glasa, M. 2013. Unfolding the secrets of plum pox virus: from epidemiology to genomics. Acta Virol. vol. 57, no. 2, p. 217-228. PMid: 23600878 DOI:

Šubr, Z., Glasa, M. 1999. Plum pox virus capsid protein mobility in SDS-polyacrylamide gel electrophoresis. Acta Virol. vol. 43, no. 4, p. 259-262. PMid: 10749373

Šubr, Z., Pittnerová, S., Glasa, M. 2004. A simplified RT-PCR-based detection of recombinant Plum pox virus isolates. Acta Virol. vol. 48, no. 3, p. 173-176. PMid: 15595211

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution. vol. 28, no. 10, p. 2731-2739. PMid: 21546353 DOI:




How to Cite

Rozák, J. ., Predajňa, L. ., Gálová, Z. ., & Glasa, M. . (2013). Detection and characterisation of Plum pox virus (PPV) isolates from Eastern Slovakia revealed the presence of three main viral strains. Potravinarstvo Slovak Journal of Food Sciences, 8(1), 1–7.

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