Application of nisin into slovak fermented salami Púchov


  • Andrea Laukova Institute of Animal Physiology Slovak Academy of Sciences, Šoltésovej 4-6, 04001 Košice
  • Peter Turek University of Veterinary Medicine and Pharmacy, Komenského 73, 048 01 Košice



nisin, Listeria innocua, fermented salami


Púchov salami is favorite fermented salami among Slovak consumers. Nisin is the only bacteriocin accepted by European Commission for a commercial use as additive for food preservation (although not commonly used in meat products). Because of its possibility to prolonge shelf-life of the products and its antimicrobial activity, its effect in dry fermented Slovak salami Púchov experimentally inoculated with Listeria innocua Li1 strain was checked. The initial number of L. innocua Li1 in the inoculated salami mixtures was 104 CFU/g (log 10; 4.04  ± 0 07). After nisin addition, the count of Li1 strain in the meat samples (inoculated with Li1 and treated by nisin) was 1.36±0.07 CFU/g; difference 2.68 logarithmic cycle was noted between Li and Li/Ni samples. At day 2, the difference 3.23 log cycle was detected between Li1 and Li/Ni samples (Li:5.46±0.08, Li/Ni: 2.14±0.07 CFU/g); at weeks 3, 4, it was 1.69 and 1.80 log cycle. Activity of nisin itself was not recovered from the experimental salamis by the analytical method; however, its inhibitory effect was shown by Li1 count decrease. The pH in  salamis during processing was almost at the same level (5.52, 5.53, 5.55). Water activity was not negatively influenced. Water content in Li/Ni salamis reached almost requested levels (maximum percentage of water requested is 34 %).


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BOZIARIS, I. S., ADAMS, M. R. 2001. Temperature shock, injury and transient sensitivity to nisin in Gram negatives. In J. Appl. Microbiol., vol. 91, 2001, p. 715-724. 10.1046/j.1365-2672.2001.01433.x PMid:11576309

COFFEY, A., RYAN, M., ROSS, R. P., HILL, C., ARENDT, E., SCHWARZ, G. 1998. Use of a broad host range bacteriocin-producing Lactococcus lactis transconjugant as an alternative starter for salami manufacture. In Int. J. Food Microbiol., vol. 43, 1998, p. 231- 235. 10.1016/S0168-1605(98)00115-9 PMiD:9801199

CHUNG, K. T., DICKSON, J. S., CROUSE, J. D. 1989. Effect of nisin on growth of bacteria attached to meat. In Appl. Environ. Microbiol., vol. 55, 1989, p. 1329-1333. PMiD:2764559

DAVIES, E. A., MILNE, C. F., BEVIS, H. E., POTTER, R. W., HARRIS, J. M., WILLIAMS, G. C., THOMAS, L. V., DELVES-BROUGHTON, J. 1999. Effective use of nisin to control lactic acid bacterial spoilage in vacuum-packed bologna-type sausage. In J. Food Prot., vol. 62, 1999, p. 1004-1010. PMiD:10492474

DELVES-BROUGHTON, J., BLACKBURN, P., EVANS, R. J., HUGENHOLTZ, J. 1996. Applications of the bacteriocin, nisin. In Ant. Leeuwenhoek., vol. 69, 1996, p. 193-202. PMiD:8775979

EUROPEAN ECONOMIC COMMUNITY. 1983. EEC Commission Directive 83/463/EEC

GANZLE, M. G., WEBER, S., HAMMES, W. P. 1999. Effect of ecological factors on the inhibitory spectrum and activity of bacteriocins. In Int. J. Food Microbiol., vol. 46, 1999, p. 207-217. PMiD:20140721

KIŠIDAYOVÁ, S., LAUKOVÁ, A., JALČ, D. 2009. Comparison if nisin and monensin effects on ciliate and selected bacterial populations in artificial rumen. In Folia Microbiol., vol. 54, p. 527-532. PMiD:20140721

LAUKOVÁ, A., JURIŠ, P. 1998. Antagonistic effect of lantibiotic nisin against bacteria isolated from wastewater. In Proceedings of the International Conference Ecology and Veterinary Medicine IV., Košice, 13-16 May, p. 115-120.

LAUKOVÁ, A., CZIKKOVÁ, S. 1999. The use of enterocin CCM 4231 in soy milk to control the growth of Listeria monocytogenes and Staphylococcus aureus. In J. Appl. Microbiol., vol. 87, 1999, p. 182-186. PMiD:10432598

LAUKOVÁ, A., CZIKKOVÁ, S., DOBRÁNSKY, T., BURDOVÁ, O. 1999a. Inhibition of Listeria monocytogenes and Staphylococcus aureus by enterocin CCM 4231 in milk products. In Food Microbiol., vol. 16, 1999a., p. 93-99.

LAUKOVÁ, A., CZIKKOVÁ, S., LACZKOVÁ, S.,TUREK, P. 1999b. Use of enterocin CCM 4231 to control Listeria monocytogenes in experimentally contaminated dry fermented Hornád salami. In Int. J. Food Microbiol., vol. 52, 1999b, p. 115-119.

LAUKOVÁ, A. 2000. In vitro treatment of different isolates from cattle dung and pig slurry by nisin. In Acta Vet. Brno, vol. 69, 2000, p. 147-151.

LAUKOVÁ, A., VLAMYNCK, G., CZIKKOVÁ, S. 2001a. Effect of enterocin CCM 4231 on Listeria monocytogenes in Saint-Paulin cheese. In Folia Microbiol., vol., 46, 2001a, p. 157-160. PMiD:11501405

LAUKOVÁ, A., ŠTYRIAK, I., MAREKOVÁ, M. 2001b. In vitro antagonistic effect of nisin on fecal enterococci and staphylococci. In Vet. Med. Czech, vol. 46, 2001b, p. 237-240.

LAUKOVÁ, A., SIMONOVÁ, M., STROMPFOVÁ, V. 2010. Staphylococcus xylosus S03/1M/1/2, bacteriocin - producing meat starter culture or additive. In Food Control, vol. 21, 2010, p. 970-973.

LAUKOVÁ, A., TUREK, P. 2011. Effect of enterocin 4231 in Slovak fermented salami Púchov after its experimental inoculation with Listeria innocua Li1. In Acta Sci. Pol. Technol. Aliment., vol. 10, 2011, p. 423-431. PMiD:22230924

MAISNIER-PATIN, S., DESCHAMPS, N., TATINI, S. R., RICHARD, J. 1992. Inhibition of Listeria monocytogenes in Camembert cheese made with a nisin -producing starter. In Lait, vol. 72, 1992, p. 249-263.

RAYMAN, K., MALIK, N. , HURST, A. 1983. Failure of nisin to inhibit outgrowth of Clostridium botulinum in a model cured meat system. In Appl. Environ. Microbiol., vol. 46, 1983, p. 1450-1452. PMiD:6362566

SAHL, H. G. 1991. Pore formation in bacterial membranes by cationic lantibiotics. In : Jung, G. & Sahl, H.G. (Eds) Nisin and Novel Lantibiotics. Proceedings of the First International Workshop on Lantibiotics, p. 347-358. Escom, Leiden, The Netherlands.

SAHL, H. G. .BIERBAUM, G. 1998. Lantibiotics: Biosynthesis and Biological activities of Uniquely modified peptides from Gram-positive bacteria. In Ann. Rev. Microbiol., vol. 52, 1998, p. 41-79. PMiD:9891793

SCANNEL, A. G. M., HILL, C., BUCKLEY, D. J., ARENDT, E. K. 1997. Determination of the influence of organic acids and nisin on shelf-life and microbiological safety aspects of fresh pork sausage. In J. Appl. Microbiol., vol. 83, 1997, p. 407-412. PMiD:9351222

SERGIOU, V. A., THOMAS, L. V., ADAMS, M. R. 2006. Interactions of nisin with glutathione in a model protein system and meat. In J. Food Prot., vol. 69, 2006, p. 951-956. PMiD:16629047

SOBRINO-LÓPEZ, A., MARTIN-BELLOSO, O. 2008. Use of nisin and other bacteriocins for preservation of dairy products. In Int. Dairy J., vol.18, 2008, p. 329-343.




How to Cite

Laukova, A. ., & Turek, P. . (2012). Application of nisin into slovak fermented salami Púchov. Potravinarstvo Slovak Journal of Food Sciences, 6(4), 32–35.

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