Occurrence of some pathogenity factors in coagulase negative Staphylococci isolated from mastitis milk in dairy cows

Authors

  • Milan Vasiľ Department of Nutrition, Dietetics and Animal Husbandry, Institute of Animal Husbandry, University of Veterinary Medicine and Pharmacy, Komenského 71, 041 81 Košice
  • Juraj Elečko Department of Nutrition, Dietetics and Animal Husbandry, Institute of Animal Husbandry, University of Veterinary Medicine and Pharmacy, Komenského 71, 041 81 Košice
  • František Zigo Department of Nutrition, Dietetics and Animal Husbandry, Institute of Animal Husbandry, University of Veterinary Medicine and Pharmacy, Komenského 71, 041 81 Košice
  • Zuzana Farkašová Department of Nutrition, Dietetics and Animal Husbandry, Institute of Animal Husbandry, University of Veterinary Medicine and Pharmacy, Komenského 71, 041 81 Košice

DOI:

https://doi.org/10.5219/186

Keywords:

mastitis, dairy cows, coagulase-negative staphylococci, virulence factors

Abstract

Experiment we carried out on the herd of 115 Slovak Pied dairy cows. Were realized three collections of individual milk samples for isolation and identification of coagulase-negative staphylococci (CNS). We collected 341 milk samples. Coagulase - negative staphylococci were isolated in 18.8 % (64). Highest number of CNS we isolated from acute, subclinical, subacute and latent forms of mastitis, respectively. S. warneri (2.88 %), S. chromogenes (17.19 %), S. cohnii (15.63 %) and S. xylosus (14.06 %) was isolated in highest percentage. Was detected presence of 7 virulence factors, include production of staphylococcal enterotoxins, and resistance for antibiotics. S. chromogenes, S. warneri and S. xylosus were detected as most virulent species carrying most virulence factors, and were resistant for most species of tested antibiotics (S. xylosus resistant for N, NV, L, P, E; S. chromogenes, S. sciuri for 4 species of antibiotics). There pathogens were isolated from acute forms of mastitis. 

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References

ČAPLA, J., ZAJÁC, P., VACZLOVÁ, Z., GOLIAN, J. 2008. Mikrobiologická kvalita surového kravského mlieka vo výrobnej oblasti. In: Bezpečnosť a kontrola potravín, Zborník vedeckých prác II. diel, SPU Nitra, 2008, p. 227- 231.

EBRAHIMI, A., AKHAVAN TAHERI, M. 2009. Characteristics of staphylococci isolated from clinical and subclinical mastitis cows in Shahrekord, Iran. In Iranian Journal of Veterinary Research, vol. 10, 2009, no. 3, p. 270-277.

HAVERI, M., ROSLÖF, A., PYÖRÄLÄ, S. 2007. Virulence genes of bovine Staphylococcus aureus from persistent and nonpersistent intramammary infections with different clinical characteristics. In J. Appl. Microbiol., vol. 103, 2007, p. 993-1000 https://doi.org/10.1111/j.1365-2672.2007.03356.x PMid:17897203 DOI: https://doi.org/10.1111/j.1365-2672.2007.03356.x

KALMUS, P., AASMÄE, B., KÄRSSIN, A., ORRO, T., KASK, K. 2011. Udder pathogens and their resistance to antimicrobial agents in dairy cows in Estonia. In Acta Veterinaria Scandinavica, vol. 53, 2011, p. 4-10. https://doi.org/10.1186/1751-0147-53-4 PMid:21299911 DOI: https://doi.org/10.1186/1751-0147-53-4

KIRKAN, S., GOKSOY,E. O., KAYA, O. 2005. Identification and Antimicrobial Susceptibility of Staphylococcus aureus and coagulase negative staphylococci from bovine mastitis in the Aydin region of Turkey. In Turk J. Vet. Anim. Sci., vol. 29, 2005, p. 791-796.

KURODA, M., NAGASAKI, S., ITO, R., OHTA, T. 2007. Sesquiterpene farnesol as a competitive inhibitor of lipase activity of Staphylococcus aureus. In FEMS Microbiology Letters, vol. 273, 2007, no.1, p. 28-34. PMid:17559400 DOI: https://doi.org/10.1111/j.1574-6968.2007.00772.x

MELCHIOR, M. B., FINK-GREMMELS, J., GAASTRA, W. 2006. Comparative assessment of the antimicrobial susceptibility of Staphylococcus aureus isolates from bovine mastitis in biofilm versus planktonic culture. In J. Vet. Med. B. Infect. Dis. Vet. Public Health., vol. 53, 2006, no. 7, p. 326-32. PMid:16930277 DOI: https://doi.org/10.1111/j.1439-0450.2006.00962.x

MONIRI, R., DASTEHGOLI, K., AKRAMIAN, A. 2007. Increasing Resistant CNS in Bovine Clinical Mastitis. In Pakistan J. Biolog. Sci., vol. 10, 2007, no. 15, p. 2465-2469. PMid:19070115 DOI: https://doi.org/10.3923/pjbs.2007.2465.2469

NASCIMENTO, J. S., FUGUNDES, P. C., BRITO, A. V., DOS SANTOS, K. R., BASTOS, M. C. 2005. Production of bacteriocins by coagulase-negative staphylococci involved in bovine mastitis. In Veterinary Microbiology, vol. 106, 2005, p. 61-71. https://doi.org/10.1016/j.vetmic.2004.10.014 PMid:15737474 DOI: https://doi.org/10.1016/j.vetmic.2004.10.014

PERSSON, Y., ANN-KRISTIN J. NYMAN, A-K. J., GRÖNLUND-ANDERSSON, U. 2011. Etiology and antimicrobial susceptibility of udder pathogens from cases of subclinical mastitis in dairy cows in Sweden. In Acta Veterinaria Scandinavica, vol. 53, 2011, 36-43. https://doi.org/10.1186/1751-0147-53-36 PMid:21649936 DOI: https://doi.org/10.1186/1751-0147-53-36

PYÖRÄLÄ, S., TAPONEN, S. 2009. Coagulase-negative staphylococci - Emerging mastitis pathogens. In Veterinary Microbiology, vol. 134, 2009, no. 1-2, p. 3-8. PMid:18848410 DOI: https://doi.org/10.1016/j.vetmic.2008.09.015

OTTO, M. 2004. Virunce factors of the coagulase-negative staphylococci. In Frontiers in Bioscience, 9, January 1, 2004, p. 841-863. https://doi.org/10.2741/1295 PMid:14766414 DOI: https://doi.org/10.2741/1295

ZAJÁC, P., GOLIAN, J., ČAPLA, J. 2008. Rezídua inhibičných látok v surovom kravskom mlieku v Slovenskej republike v roku 2007. In Bezpečnosť a kontrola potravín, Zborník vedeckých prác II. diel, SPU Nitra, 2008, p. 274-277.

ZAJÁC, P., GOLIAN, J., SOKOL, J. 2004. Výsledky vyšetrenia surového kravského mlieka na RIL v slovenskej republike a rok 2001, 2002, 2003. In: Zborník prednášok a posterov, Hygiena alimentorum XXV, UVL Košice 2004, p. 228-232.

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Published

2012-03-01

How to Cite

Vasiľ, M. ., Elečko, J. ., Zigo, F. ., & Farkašová, Z. . (2012). Occurrence of some pathogenity factors in coagulase negative Staphylococci isolated from mastitis milk in dairy cows. Potravinarstvo Slovak Journal of Food Sciences, 6(2), 60–63. https://doi.org/10.5219/186

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