Polyphenol components and antioxidant activity of Thymus spp.
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https://doi.org/10.5219/1715Keywords:
Thymus, polyphenol content, antioxidant activityAbstract
This scientific work was aimed to evaluate the antioxidant potential of aromatic plants of Thymus spp. in the East of Ukraine. These plants are known as medicinal and food around the world. All antioxidant parameters were investigated spectrophotometrically: total content of polyphenols (TPC), the total content of phenolic acids (TPAC), the total content of flavonoids (TFC), molybdenum reducing power of extracts (MRP), and antioxidant activity by DPPH method (DPPH). Investigation of ethanolic extracts demonstrated that TPC varied from 57.89 to 123.67 mg/g gallic acid equivalent (GAE) DW for Th. pulegioides, from 61.43 to 168.18 mg GAE/g for Th. serpyllum, and from 47.36 to 115.67 mg GAE/g for Th. vulgaris. TPAC ranged from 27.36 to 50.22 mg/g caffeic acid equivalent (CAE) DW for Th. pulegioides, from 28.58 to 59.62 mg CAE/g for Th. serpyllum, and from 22.95 to 53.82 mg CAE/g for Th. vulgaris. TFC was determined in a range from 29.88 to 61.23 mg/g quercetin equivalent (QE) DW for Th. pulegioides, from 36.0 to 82.43 mg QE/g for Th. serpyllum, and from 24.59 to 55.41 mg QE/g for Th. vulgaris. MRP was detected in the range of 94.65 – 204.76 mg/g Trolox equivalent (TE) DW for Th. pulegioides, 96.06 – 219.0 mg TE/g for Th. serpyllum, and 87.56 – 215.43 mg TE/g for Th. vulgaris. The antioxidant activity of extracts by the DPPH method was 6.34 – 9.23 mg TE/g for Th. pulegioides, 8.11 – 9.21 mg TE/g for Th. serpyllum, and 4.97 – 9.53 mg TE/g for Th. vulgaris. It was established that polyphenol accumulation depended on the growth stage and species. For all species was found a strong correlation between TPC and TFC (r = 0.938, 0.908, and 0.854). Investigated Thymus spp. are a valuable source of antioxidants that can be used in pharmacological studies and the food industry.
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Proestos, C., & Varzakas, T. (2017). Aromatic plants: antioxidant capacity and polyphenol characterization. In Foods (vol. 6, Issue 4, pp. 28). MDPI AG. https://doi.org/10.3390/foods6040028 DOI: https://doi.org/10.3390/foods6040028
Honcharenko, V., Tkachenko, H., Nachychko, V., Prokopiv, A., & Osadowski, Z. (2018). Total antioxidant capacity in the muscle tissue of rainbow trout (Oncorhynchus mykiss Walbaum). In Agrobiodiversity for Improving Nutrition, Health and Life Quality (vol. 2, pp. 223–233). https://doi.org/10.15414/agrobiodiversity.2018.2585-8246.223-233 DOI: https://doi.org/10.15414/agrobiodiversity.2018.2585-8246.223-233
Grygorieva, O., Vergun, O., Klymenko, S., Zhurba, M., Horčinová Sedláčková, V., Ivanišová, E., & Brindza, J. (2020). Estimation of phenolic compounds content and antioxidant activity of leaves extracts of some selected non-traditional plants. In Potravinarstvo Slovak Journal of Food Sciences (vol. 14, Issue 1, pp. 501–509). HACCP Consulting. https://doi.org/10.5219/1314 DOI: https://doi.org/10.5219/1314
Horčinová Sedláčková, V., Grygorieva, O., Fatrcová Šramková, K., Vergun, O., Vinogradova, Y., Ivanišová, E., & Brindza, J. (2018). The morphological and antioxidant characteristics of inflorescences within wild-growing genotypes of elderberry (Sambucus nigra L.). In Potravinarstvo Slovak Journal of Food Sciences (vol. 12, Issue 1, pp. 444–453). HACCP Consulting. https://doi.org/10.5219/919 DOI: https://doi.org/10.5219/919
Nikolaieva, N., Kačániová, M., Collado González, J., Grygorieva, O., & Nôžková, J. (2019). Determination of microbiological contamination, antibacterial and antioxidant activities of natural plant hazelnut (Corylus avellana L.) pollen. In Journal of Environmental Science and Health (vol. 54, Issue 6, pp. 525–532). Informa UK Limited. https://doi.org/10.1080/03601234.2019.1603756 DOI: https://doi.org/10.1080/03601234.2019.1603756
Monka, A., Grygorieva, O., Chlebo, P., & Brindza, J. (2014). Morphological and antioxidant characteristics of quince (Cydonia oblonga Mill.) and chinese quince fruit (Pseudocydonia sinensis Schneid.). In Potravinarstvo Slovak Journal of Food Sciences (vol. 8, Issue 1, pp. 333–340). HACCP Consulting. https://doi.org/10.5219/415 DOI: https://doi.org/10.5219/415
Sochor, J., Krska, B., Polak, J., & Jurikova, T. (2015). The influence of virus infections on antioxidant levels in the genetically modified plum variety "Honeysweet "(Prunus domestica L.). In Potravinarstvo Slovak Journal of Food Sciences (vol. 9, Issue 1, pp. 195–200). HACCP Consulting. https://doi.org/10.5219/420 DOI: https://doi.org/10.5219/420
Grygorieva, O., Klymenko, S., Vergun, O., Mňahončakova, E., Brindza, J., Terentjeva, M., & Ivanišova, E. (2020). Evaluation of the antioxidant activity and phenolic content of Chinese quince (Pseudocydonia sinensis Schneid.) fruit. In Acta Scientiarum Polonorum, Technologia Alimentaria (vol. 19, Issue 1, pp. 25–36). Poznan University of Life Science Institute. http://dx.doi.org/10.17306/J.AFS.2020.0738 DOI: https://doi.org/10.17306/J.AFS.0738
Vinogradova, Y., Vergun, O., Grygorieva, O., Ivanišová, E., & Brindza, J. (2020). Comparative analysis of antioxidant activity and phenolic compounds in the fruits of Aronia spp. In Potravinarstvo Slovak Journal of Food Sciences (vol. 14, pp. 393–401). HACCP Consulting. https://doi.org/10.5219/1360 DOI: https://doi.org/10.5219/1360
Ivanišová, E., Vasková, D., Zagula, G., Grynshpan, D., Savitskaya, T. A., & Kačániová, M. (2020). Phytochemical profile and biological activity of selected kind of medicinal herbs. In Potravinarstvo Slovak Journal of Food Sciences (vol. 14, Issue 1, pp. 573–579). HACCP Consulting. https://doi.org/10.5219/1370 DOI: https://doi.org/10.5219/1370
Alam, M. K., Rana, Z. H., Islam, S. N., & Akhtaruzzaman, M. (2019). Total phenolic content and antioxidant activity of methanolic extract of selected wild leafy vegetables grown in Bangladesh: a cheapest source of antioxidants. In Potravinarstvo Slovak Journal of Food Sciences (vol. 13, Issue 1, pp. 287–293). HACCP Consulting. https://doi.org/10.5219/1107 DOI: https://doi.org/10.5219/1107
Krochmal-Marczak, B., Sawicka, B. (2019). The influence of cooking on the antioxidant properties and polyphenol content in buckwheat, barley and millet groats and the transfer of the compounds to the water. In Potravinarstvo Slovak Journal of Food Sciences (vol. 13, Issue 1, pp. 759–766). HACCP Consulting. https://doi.org/10.5219/1171 DOI: https://doi.org/10.5219/1171
Škrovánková, S., Válková, D., & Mlček, J. (2020). Polyphenols and antioxidant activity in pseudocereals and their products. In Potravinarstvo Slovak Journal of Food Sciences (vol. 14, pp. 365–370). HACCP Consulting. https://doi.org/10.5219/1341 DOI: https://doi.org/10.5219/1341
Szilvássy, B., Rak, G., Sárosi, S., Novák, I., Pluhár, Z., & Abrankó, L. (2013). Polyhenols in the aqueous extracts of garden thyme (Thymus vulgaris L.) chemotypes cultivated in Hungary. In Natural Product Communications (vol. 8, Issue 5, pp. 605–608). SAGE Publications. https://doi.org/10.1177/1934578X1300800516 DOI: https://doi.org/10.1177/1934578X1300800516
Pineda-Ramírez, N., Calzada, F., Alquisiras-Burgos, I., Medina-Campos, O. N., Pedraza-Chaverri, J., Ortiz-Plata, A., Estrada, E. P., Torres, I., & Aguilera, P. (2020). Antioxidant properties and protective effects of some species of the Annonaceae, Lamiaceae, and Geraniaceae families against neuronal damage induced by excitotoxicity and cerebral ischemia. In Antioxidants (vol. 9, Issue 253). MDPI AG. https://doi.org/10.3390/antiox9030253 DOI: https://doi.org/10.3390/antiox9030253
Afonso, A. F., Pereira, O. R., & Cardoso, S. M. (2020). Health-promoting effects of Thymus phenolic-rich extracts: antioxidant, anti-inflammatory, and antitumor properties. In Antioxidants (vol. 9, 814). MDPI AG. https://doi.org/10.3390/antiox9090814 DOI: https://doi.org/10.3390/antiox9090814
Taghouti, M., Martins-Gomes, C., Schafer, J., Santos, J. A., Bunzel, M., Nunes, F. M., & Silva, A. M. (2020). Chemical characterisation and bioactivity of extracts from Thymus mastishina: a Thymus with a distinct salvianolic acid composition. In Antioxidants (vol. 9, Issue 1, Article 34, pp. 1–18). MDPI AG. https://doi.org/10.3390/antiox9010034 DOI: https://doi.org/10.3390/antiox9010034
Honcharenko, V., Tkachenko, H., Nachychko, V., Prokopiv, A., & Osadowski, Z. (2018). The antibacterial activities of some Thymus (Lamiaceae) representatives against Salmonella enteriditis strain locally isolated. In Agrobiodiversity for Improving Nutrition, Health and Life Quality (vol. 2, pp. 212–222). https://doi.org/10.15414/agrobiodiversity.2018.2585-8246.212-222 DOI: https://doi.org/10.15414/agrobiodiversity.2018.2585-8246.212-222
Honcharenko, V., Tkachenko, H., Nachychko, V., Prokopiv, A., & Osadowski, Z. (2019). Antibacterial properties of ethanolic extracts obtained from leaves of some Thymus L. (Lamiaceae) representatives against Acinetobacter baumanii. In Agrobiodiversity for Improving Nutrition, Health and Life Quality (vol. 3, pp. 14–24). https://doi.org/10.15414/agrobiodiversity.2019.2585-8246.014-024 DOI: https://doi.org/10.15414/agrobiodiversity.2019.2585-8246.014-024
Prokopiv, A., Tkachenko, H., Honcharenko, V., Nachychko, V., Kurhaluk, N., & Osadowski, Z. (2019). Cytotoxic effect of leaf extracts of some Thymus L. (Lamiaceae) representatives using in vitro human blood model. In Agrobiodiversity for Improving Nutrition, Health and Life Quality (vol. 3, pp. 451–464). https://doi.org/10.15414/agrobiodiversity.2019.2585-8246
Almanea, A., El-Aziz, G. S. A., & Ahmed, M. M. M. (2019). The potential gastrointestinal health benefits of Thymus vulgaris essential oil: a review. In Biomedical and Pharmacology Journal (vol. 12, Issue 4, pp. 1793–1799). Oriental Scientific Publishing Company. https://dx.doi.org/10.13005/bpj/1810 DOI: https://doi.org/10.13005/bpj/1810
Grosso, C., Figueiredo, A. C., Buriello, J., Mainar, A. M., Urieta, J. S., Barroso, J. C., Coelho, J. A., & Palavra, A. M. F. (2010). Composition and antioxidant activity of Thymus vulgaris volatiles: comparison between supercritical fluid extraction and hydrodistillation. In Journal of Separation Science (vol. 33, pp. 2211–2218). Wiley. https://doi.org/10.1002/jssc.201000192 DOI: https://doi.org/10.1002/jssc.201000192
Wesołowska, A., Jadczak, D. (2019). Comparison of the chemical composition of essential oils isolated from two thyme (Thymus vulgaris L.) cultivars. In Notulae Botanicae Horti Agrobotanici (vol. 47, Issue 3). University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. https://doi.org/10.15835/nbha47311451 DOI: https://doi.org/10.15835/nbha47311451
Raal, A., Paaver, U., Arale, E., & Orav, A. (2004). Content and composition of the essential oil of Thymus serpyllum L. growing wild in Estonia. In Medicina (Kaunas) (vol. 40, Issue 8, pp. 795–800).
Samein, N. M., & Kamel, F. H. (2019). Extraction of compounds from thyme leaves and their antimicrobial activity. In Diyala Journal of Agricultural Sciences (vol. 11, Issue 1, pp. 18–24).
Prasanth Reddy, V., Ravi Vital, K., Varsha, P. V., & Satyam, S. (2014). Review on Thymus vulgaris L. traditional uses and pharmacological properties. In Medicinal and Aromatic Plants (vol. 3, Issue 3). OMICS Publishing Group. https://doi.org/10.4172/2167-0412.1000164 DOI: https://doi.org/10.4172/2167-0412.1000164
Jarić, S., Mitrović, M., & Pavlović, P. (2015). Review on ethnobotanical, phytochemical, and pharmacological study on Thymus serpyllum L. In Evidence-Based Complementary and Alternative Medicine (vol. 2015, 101978). Hindawi Limited. https://dx.doi.org/10.1155/2015/101978 DOI: https://doi.org/10.1155/2015/101978
Kindl, M., Blažeković, B., Bucar, F., & Vladimir-Knežević, S. (2015). Antioxidant and anticholinesterase potential of six Thymus species. In Evidence-Based Complementary and Alternative Medicine (vol. 2015, 403950). Hindawi Limited. https://dx.doi.org/10.1155/2015/403950 DOI: https://doi.org/10.1155/2015/403950
Achour, S., Khelifi, E., Attia, Y., Ferjani, E., & Hellal, A. N. (2012). Concentration of antioxidant polyphenols from Thymus capitatus extracts by membrane process technology. In Journal of Food Science (vol. 77, no. 6, pp. 703–709). Wiley. https://doi.org/10.1111/j.1750-3841.2012.02696.x DOI: https://doi.org/10.1111/j.1750-3841.2012.02696.x
Borugă, O., Jianu, C., Mişcă, C., Goleţ, I., Gruia, A. T., & Horhat, F. G. (2014). Thymus vulgaris essential oil: chemical composition and antimicrobial activity. In Journal of Medicine and Life (vol. 7, Issue 3, pp. 56–60).
Verma, R. S., Verma, R. K., Chauhan, A., & Yadav, A. K. (2011). Seasonal variation in essential oil content and composition on Thyme, Thymus serpyllum L. cultivated in Uttarakhand Hills. In Indian Journal of Pharmaceutical Sciences (vol. 73, Issue 2, pp. 233–235). OMICS Publishing Group. https://doi.org/10.4103/0250-474X.91570 DOI: https://doi.org/10.4103/0250-474X.91570
Gedicoğlu, A., Sökmen, M., & Çivit, A. (2019). Evaluation of Thymus vulgaris and Thymbra spicata essential oils and plant extracts for chemical composition, antioxidant, and antimicrobial properties. In Food Science and Nutrition (vol. 7, pp. 1704–1714). Wiley. https://doi.org/10.1002/fsn3.1007 DOI: https://doi.org/10.1002/fsn3.1007
Grigore, A., Paraschiv, I., Colceru-Mihul, S., Bubueanu, C., Draghici, E., & Ichim, M. (2010). Chemical composition and antioxidant activity of Thymus vulgaris L. volatile oil obtained by two different methods. In Romanian Biotechnological Letters (vol. 15, Issue 4, pp. 5436–5443).
El-Nekeety, A. A., Mohamed, S. R., Hathout, A. S., Hassan N. S., Aly, S. E., & Abdel-Wahhab, M. A. (2011). Antioxidant properties of Thymus vulgaris oil against aflatoxin-induced oxidative stress in male rats. In Toxicon (vol. 57, pp. 984–991). Elsevier BV. https://doi.org/10.1016/j.toxicon.2011.03.021 DOI: https://doi.org/10.1016/j.toxicon.2011.03.021
Juárez-Rosete, C. R., Aguilar-Castillo, J. A., & Rodriguez-Mendoza, M. N. (2014). Fertiliser source in biomass production and quality of essential oils of thyme (Thymus vulgaris L.). In European Journal of Medicinal Plants (vol. 4, Issue 7, pp. 865–871). Sciencedomain International. https://dx.doi.org/10.9734/EJMP/2014/9169 DOI: https://doi.org/10.9734/EJMP/2014/9169
Lozienė, K., Venskutonis, P. R., Šipailienė, A., & Labokas, J. (2007). Radical scavenging and antibacterial properties of the extracts from different Thymus pulegioides L. chemotypes. In Food Chemistry (vol. 103, pp. 546–559). Elsevier BV. https://doi.org/10.1016/j.foodchem.2006.08.027 DOI: https://doi.org/10.1016/j.foodchem.2006.08.027
Taghouti, M., Martins-Gomes, C., Schäfer, J., Félix, L. M., Santos, J. A., Bunzel, M., Nunes, F. M., & Silva, A. M. (2018). Thymus pulegioides L. as a rich source of antioxidant, antiproliferative and neuroprotective phenolic compounds. In Food and Function (vol. 9, pp. 3617–3629). Royal Society of Chemistry. https://doi.org/10.1039/C8FO00456K DOI: https://doi.org/10.1039/C8FO00456K
Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagent. In American Journal of Enology and Viticulture (vol. 16, Issue 3, pp. 144–158).
Farmakopea Polska. 1999. The Polish Farmaceutical Society. Available at: http://www.ptfarm.pl/?pid=1&language=en
Pękal, A., & Pyrzynska, K. (2014). Evaluation of aluminium complexation reaction for flavonoid content assay. In Food Analytical Methods (vol. 7, Issue 9, pp. 1776–1782). Springer Science and Business Media LLC. https://doi.org/10.1007/s12161-014-9814-x DOI: https://doi.org/10.1007/s12161-014-9814-x
Shafii, Z. A., Basri, M., Malek, E. A., & Ismail, M. (2017). Phytochemical and antioxidant properties of Manilkara zapota (L.) P royen fruit extracts and its formulations for cosmeceutical application. In Asian Journal of Plant Science and Research (vol. 7, Issue 3, pp. 29–41).
Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. In Analytical Biochemistry (vol. 269, Issue 2, pp. 337–241). Elsevier BV. http://doi.org/10.1006/abio.1999.4019 DOI: https://doi.org/10.1006/abio.1999.4019
Sánchéz-Moreno, C., Larrauri, A., & Saura-Calixto, F. (1998). A procedure to measure the antioxidant efficiency of polyphenols. In Journal of the Science of Food and Agriculture (vol. 76, Issue 2, pp. 270–276). Wiley. https://doi.org/10.1002/(SICI)1097-0010(199802)76:2<270::AID-JSFA945>3.0.CO;2-9 DOI: https://doi.org/10.1002/(SICI)1097-0010(199802)76:2<270::AID-JSFA945>3.0.CO;2-9
Gupta, D. (2015). Methods for determination of antioxidant capacity: a review. In International Journal of Pharmaceutical Sciences and Research (vol. 6, Issue 2, pp. 546–566). https://dx.doi.org/10.13040/IJPSR.0975-8232.6(2).546-66 DOI: https://doi.org/10.13040/IJPSR.0975-8232.6(2).546-66
Adámková, A., Kouřimská, L., & Kadlecová, B. (2015). The effect of drying on antioxidant activity of selected Lamiaceae herbs. In Potravinarstvo Slovak Journal of Food Sciences (vol. 9, Issue 1, pp. 252–257). HACCP Consulting. https://doi.org/10.5219/474 DOI: https://doi.org/10.5219/474
Armatu, A., Colceru-Mihul, S., Bubueanu, C., Draghica, E., & Pirvu, L. (2010). Evaluation of antioxidant and free radical scavenging potential of some Lamiaceae species growing in Romania. In Romanian Biotechnological Letters (vol. 15, Issue 3, pp. 5274–5280).
Msaada, K., Tammar, S., Salem, N., Bachrouch, O., Sriti, J., Hammami, M., Selmi, S., Azaier, S., Hadj-Brahim, A., Sane, K. A., Limam, F., & Marzour, B. (2016). Chemical composition and antioxidant activities of Tunisian Thymus capitatus L. methanolic extracts. In International Journal of Food Properties (vol. 19, pp. 1381–1390). Informa UK Limited. https://doi.org/10.1080/10942912.2015.1082138 DOI: https://doi.org/10.1080/10942912.2015.1082138
Aouam, I., Alki, Y. E., Taleb, M., Taroq, A., Kamari, F. E., Lyoissi, B., & Abdellaoui, A. 2019. Antioxidant capacities and total phenolic contents of Thymus riatarum. In Materials Today: Proceedings (vol. 13, pp. 579–586). https://doi.org/10.1016.j.matpr.2019.04.016 DOI: https://doi.org/10.1016/j.matpr.2019.04.016
Köksal, E., Bursal, E., Gülçin, I., Korkmaz, M., Ģağlayan, C., Gören, A. C., & Alwasel, S. H. (2017). Antioxidant activity and polyphenol content of Turkish thyme (Thymus vulgaris) monitored by liquid chromatography and tandem mass spectrometry. In International Journal of Food Properties (vol. 20, Issue 3, pp. 514–525). Informa UK Limited. https://dx.doi.org/10.1080/10942912.2016.1168438 DOI: https://doi.org/10.1080/10942912.2016.1168438
Roby, M. H. H., Sarhan, M. A., Selim, K. A.-H., & Khalel, K. I. (2013). Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Th. vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts. In Industrial Crops and Products (vol. 43, pp. 827–831). Elsevier BV. https://dx.doi.org/10.1016/j.indcrop.2012.08.029 DOI: https://doi.org/10.1016/j.indcrop.2012.08.029
Mňahončaková, E., Vergun, O., Grygorieva, O., Svydenko, L., Horčinová Sedlačková, V., Hrušová, M., Brindza, J., & Ivanišová, E. (2019). Antioxidant potential of Lamiaceae herbs from Botanical Garden of Slovak University of Agriculture in Nitra. In Agrobiodiversity for Improving Nutrition, Health and Life Quality (vol. 3, pp. 462–472). https://doi.org/10.15414/agrobiodiversity.2019.2585-8246.462-472
Oubihi, A., Hosni, H., Nounah, I., Ettouil, A., Harhar, H., Alaoui, K., Ouhssine, M., & Guessous, Z. (2020). Phenolic content, antioxidant activity, anti-inflammatory potential, and acute toxicity study of Thymus leptobotrys Murb. extracts. In Biochemistry Research International (vol. 2020, 8823209, pp. 1–7). Hindawi Limited. https://doi.org/10.1155/2020/8823209 DOI: https://doi.org/10.1155/2020/8823209
Sarfaraz, D., Rahimmalek, M., & Saeidi, G. (2021). Polyphenolic and molecular variation in Thymus species using HPLC and SRAP analyses. In Scientific Reports (vol. 11, 5019). Springer Science and Business Media LLL. https://doi.org/10.1038/s41598-021-84449-6 DOI: https://doi.org/10.1038/s41598-021-84449-6
Vábková, J., Neugebauerová, J. (2012). Determination of total phenolic content, total flavonoid content and FRAP in culinary herbs in relation to harvest time. In Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis (vol. 60, Issues 1, pp. 167–171). Mendel University Press. https://doi.org/10.11118/actaun201260010167 DOI: https://doi.org/10.11118/actaun201260010167
Pereira, O. R., & Cardoso, S. M. (2013). Overview on Mentha and Thymus polyphenols. In Current Analitical Chemistry (vol. 9, pp. 382–396). Bentham Science Publishers Ltd. https://10.2174/1573411011309030008 DOI: https://doi.org/10.2174/1573411011309030008
Rasouli, H., Farzaei, M. H., & Khodarahmi, R. (2017). Polyphenols and their benefits: a review. In International Journal of Food Properties (vol. 20, Issue 2, pp. 1700–1741). Informa UK Limited. https://doi.org/10.1080/10942912.2017.1354017 DOI: https://doi.org/10.1080/10942912.2017.1354017
Aadesariya, M. K., Ram, V. R., & Dave, P. N. (2017). Evaluation of antioxidant activities by use of various extracts from Abutilon pannosum and Grewia tenax in the Kachchh region. In MOJ Food Processing and Technology (vol. 5, Issue 1, pp. 216–230). MedCrave Group, LLC. https://doi.org/10.15406/mojfpt.2017.04.00116 DOI: https://doi.org/10.15406/mojfpt.2017.05.00116
Vergun, O., Svydenko, L., Grygorieva, O., Shymanska, O., Rakhmetov, D., Brindza, J., & Ivanišová, E. (2019). Antioxidant capacity of plant raw material of Scutellaria baicalensis Georgi. In Potravinarstvo Slovak Journal of Food Sciences (vol. 13, Issue 1, pp. 614–621). HACCP Consulting. https://doi.org/10.5219/1090 DOI: https://doi.org/10.5219/1090
Marinova, G., & Batchvarov, V. (2011). Evaluation of the methods for determination of the free radical scavenging activity by DPPH. In Bulgarian Journal of Agricultural Science (vol. 17, Issue 1, pp. 11–24).
Nickavar, B., & Esbati, N. (2021). Evaluation of the antioxidant capacity and phenolic content of three Thymus species. In Journal of Acupuncture and Meridian Studies (vol. 5, Issue 3, pp. 119–125). Medical Association of Pharmacopuncture Institute. https://doi.org/10.1016/j.jams.2012.03.003 DOI: https://doi.org/10.1016/j.jams.2012.03.003
Nadia, Z., & Rachid, M. (2013). Antioxidant and antibacterial activities of Thymus vulgaris L. In Medicinal and Aromatic Plant Research Journal (vol. 1, Issue 1, pp. 5–11).
Öztürk, N. (2015). Phenolic composition and antioxidant activity of the different extracts from Thymus longicaulis C. Presl. subsp. longicaulis var. longicaulis and T. longicaulis C. Presl. subsp. longicaulis var. subisophyllus growing in Turkey. In Pakistan Journal of Pharmaceutical Sciences (vol. 28, Issue 2, pp. 465–472).
Mankovecký, J., Galovičová, L., & Kačaniová, M. (2021). The chemical composition of two kinds of grape juice with medicinal plant addition. In Potravinarstvo Slovak Journal of Food Sciences (vol. 15, Issue 1, pp. 1082–1092). HACCP Consulting. https://doi.org/10.5219/1692 DOI: https://doi.org/10.5219/1692
Chizzola, R., Michitsch, H., & Franz, Ch. (2008). Antioxidative properties of Thymus vulgaris leaves: comparison of different extracts and essential oil chemotypes. In Journal of Agricultural and Food Chemistry (vol. 56, pp. 6897–6904). American Chemical Society. https://doi.org/10.1021/jf800617g DOI: https://doi.org/10.1021/jf800617g
Wisam, S. U., Nahla, T. K., & Tariq, N. M. (2018). Antioxidant activities of Thyme extracts. In Pakistan Journal of Nutrition (vol. 17, Issue 1, pp. 46–50). Science Alert. https://doi.org/10.3923/pjn.2018.46.50 DOI: https://doi.org/10.3923/pjn.2018.46.50
Alu’datt, M. H., Rababah, T., Johargy, A., Gammoh, S., Ereifej, K., Alhamad, M. N., Brewer, M. S., Saati, A. A., Kubow, S., & Rawshdeh, M. (2016). Extraction, optimisation and characterisation of phenolics from Thymus vulgaris L.: phenolic content and profiles in relation to antioxidant, antidiabetic and antihypertensive properties. In International Journal of Food Science and Technology (vol. 51, pp. 720–730). Wiley. https://doi.org/10.1111/ijfs.12944 DOI: https://doi.org/10.1111/ijfs.12944
Jovanović, A., Skrt, M., Petrović, P., Častran, I., Zdunič, G., Šavikin, K., & Bugarski, B. (2019). Ethanol thymus serpyllum extracts: evaluation of extraction conditions via total polyphenol content and radical scavenging activity. In Lekovite Sirovine (vol. 39, pp. 23–29). Centre for Evaluation in Education and Science (CEON/CEES). http://dx.doi.org/10.5937/leksir1939023J DOI: https://doi.org/10.5937/leksir1939023J
Kontogiorgis, C., Ntella, M., Mpompou, L., Karallaki, F., Athanasios, P., Hadjipavlou-Litina, D., & Lazari, D. (2016). Study of the antioxidant activity of Thymus sibthorpii Bentham (Lamiaceae). In Journal of Enzyme Inhibition and Medicinal Chemistry (vol. 31, pp. 154–159). https://doi.org/10.1080/14756366.2016.1222583 DOI: https://doi.org/10.1080/14756366.2016.1222583
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