Antioxidant capacity of plant raw material of Scutellaria baicalensis Georgi

Authors

  • Olena Vergun M. M. Gryshko National Botanical Gardens of Ukraine National Academy of Sciences, Timiryazevska 1, 01014 Kyiv, Ukraine, Tel.: +380975398541 https://orcid.org/0000-0003-2924-1580
  • Liudmyla Svydenko Experimental Facility “Novokakhovska” of Rice Research Institute of Ukrainian Academy of Agrarian Sciences, Sadova 1, 74 999 Plodove, Kherson region, Ukraine, Tel.: +380991215376
  • Olga Grygorieva M. M. Gryshko National Botanical Gardens of Ukraine National Academy of Sciences, Timiryazevska 1, 01014 Kyiv, Ukraine, Tel.: +380671988082
  • Oksana Shymanska M. M. Gryshko National Botanical Gardens of Ukraine National Academy of Sciences, Timiryazevska 1, 01014 Kyiv, Ukraine, Tel.: +380982284804 https://orcid.org/0000-0001-8482-5883
  • Dzhamal Rakhmetov M. M. Gryshko National Botanical Gardens of Ukraine National Academy of Sciences, Timiryazevska 1, 01014 Kyiv, Ukraine, Tel.: +380503561930
  • Ján Brindza Slovak University of Agriculutre in Nitra, Faculty of Agrobiology and Food Resources, Institute of Biological Conservation and biosafety, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414787
  • Eva Ivanišová Slovak University of Agryculture in Nitra, Faculty of Biotechnology and Food Resources, Department of Plant Storage and Processing, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414421

DOI:

https://doi.org/10.5219/1090

Keywords:

Scutellaria baicalensis, antioxidant activity, polyphenols, flavonoids, phenolic acids

Abstract

The aim of this study was to evaluate antioxidant capacity of Scutellaria baicalensis Georgi from two regions of Ukraine: Kyiv city (M. M. Gryshko National Botanical Garden of NAS of Ukraine (NBG)) and Kherson region (Experimental Facility “Novokakhovska” of Rice Research Institute of Ukrainian Academy of Agrarian Sciences (EFN of RRI)). Observation of plants and biochemical analyses conducted with plants collected in the stage of flowering. In study investigated and compared above-ground part of plants and separated organs: inflorescences, stems, leaves. Measured morphometric parameters (height of plants, length, and width of leaves, length, and diameter of inflorescence, the diameter of the stem) showed that the most variable was the length of inflorescence (12.79%) for NBG sample and diameter of the stem (33.33%) for EFN of RRI sample. Ethanolic extracts were screened for the antioxidant capacity. As standards were used gallic acid for polyphenol content (GAE), quercetin for flavonoids (QE), caffeic acid for phenolic acids (CAE), Trolox for antioxidant capacity (TE). The total content of polyphenol compounds was 42.43 – 86.13 mg GAE.g-1 DW (dry weight) (NBG sample) and 28.06 – 96.76 mg GAE.g-1 DW (EFN of RRI sample). The content of flavonoids was
9.39 – 62.97 mg QE.g-1 DW (NBG sample) and 10.64 – 66.07 mg QE.g-1 DW (EFN of RRI sample). The concentration of phenolic acids was 2.60 – 16.13 mg CA.g-1 DW (NBG sample) and 12.02 – 30.12 CA.g-1 DW (EFN of RRI sample). Antioxidant activity of plant extracts was measured by DPPH assay and reducing power method. The first method indicated an antioxidant ability 8.24 – 8.56 mg TE.g-1 DW (NBG sample) and 7.63 – 8.83 mg TE.g-1 DW (EFN of RRI sample). Reducing power of extracts was 51.48 – 306.09 mg TE.g-1 DW (NBG sample) and 63.33 – 260.24 mg TE.g-1 DW (EFN of RRI sample). Very strong positive correlation identified between total polyphenol content, total flavonoid content and reducing power. Scutellaria baicalensis is a rich source of antioxidants and potential raw of further pharmacological study in Ukraine as well as in other regions for improving and enrichment of relevant production.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Adámková, A., Kouřimská, L., Kadlecová, B. 2015. The effect of drying on antioxidant activity of selected Lamiaceae herbs. Slovak Journal of Food Sciences, vol. 9, no. 1, p. 252-257. https://doi.org/10.5219/474 DOI: https://doi.org/10.5219/474

Alam, N. Md., Bristi, J. N., Rafiguzzaman, Md. 2013. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, vol. 21, p. 143-152. https://doi.org/10.1016/j.jsps.2012.05.002 DOI: https://doi.org/10.1016/j.jsps.2012.05.002

Bazzaz, F. S. B., Khayat, H. M., Emami, A. S., Asili, J., Sahebkar, A., Neishabory, J. B. 2011. Antioxidant and antimicrobial activity of methanol, dichloromethane, and ethyl acetate extracts of Scutellaria litwinowii. ScienceAsia, vol. 37, p. 327-334. https://doi.org/10.2306/scienceasia1513-1874.2011.37.327 DOI: https://doi.org/10.2306/scienceasia1513-1874.2011.37.327

Brand-Williams, W., Cuvelier, M. E., Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT – Food Science and Technology, vol. 28, no. 1, p. 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5 DOI: https://doi.org/10.1016/S0023-6438(95)80008-5

Chen, Zh.-Y., Su, Ya.-L., Bi, Y.-R., Tsang, Y. S., Huang, Y. 2000. Effect of baicalein and acetone extract of Scutellaria baicalensis on canola oil oxidation. Journal of the American Oil Chemist’s Society, vol. 77, no. 1, p. 73-78. https://doi.org/10.1007/s11746-000-0011-y DOI: https://doi.org/10.1007/s11746-000-0011-y

Cheng, Ch.-Sh., Chen, J., Tan, H.-Y., Wang, N., Chen, Z., Feng, Y. 2018. Scutellaria baicalensis and cancer treatment: recent progress and perspectives in biomedical and clinical studies. American Journal of Chinese Medicine, vol. 46, no. 1, p. 1-30. https://doi.org/10.1142/S0192415X18500027 DOI: https://doi.org/10.1142/S0192415X18500027

Cocan, I., Alexa, E., Danciu, C., Radulov, I., Galuscan, A., Obistioiu, D., Morvay, A. A., Sumalan, R. M., Poiana, M. A., Pop, G., Dehelean, C. A. 2018. Phytochemical screening and biological activity of Lamiaceae family plant extracts. Experimental and Therapeutic Medicine, vol. 15, no. 2, p. 1863-1870. https://doi.org/10.3892/etm.2017.5640 DOI: https://doi.org/10.3892/etm.2017.5640

Cole, B. I., Cao, J., Alan, R. A., Saxena, K. P., Murch, J. S. 2008. Comparison of Scutellaria baicalensis, Scutellaria lateriflora and Scutellaria racemosa: genome size, antioxidant potential and phytochemistry. Planta Medica, vol. 74, no. 4, p. 474-481. https://doi.org/10.1055/s-2008-1034358 DOI: https://doi.org/10.1055/s-2008-1034358

Farmakopea Polska. 1999. The Polish Farmaceutical Society. Available at: http://www.ptfarm.pl/?pid=1&language=en

Gabrielska, J., Oszmianski, J., Żyłka, R., Komorowska, M. 1997. Antioxidant activity of flavones from Scutellaria baicalensis in lecithin liposomes. Z. Naturforsch., vol. 52, no. 11-12, p. 817-823. https://doi.org/10.1515/znc-1997-11-1215 DOI: https://doi.org/10.1515/znc-1997-11-1215

Gaire Prasad, B., Kim, O. Y., Jin, H. Z., Park, J., Choi, H., Bu, Y., Kim, H. 2014. Neuroprotective effect of Scutellaria baicalensis flavones against global ischemic model in rats. Journal of NPA, vol. 27, no. 1, p. 1-8. https://doi.org/10.3126/jnpa.v27i1.12144 DOI: https://doi.org/10.3126/jnpa.v27i1.12144

Gao, Z., Huang, K., Yang, X., Xu, H. 1999. Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Biochimica et Biophysica Acta, vol. 1472, no. 3, p. 643-650. https://doi.org/10.1016/S0304-4165(99)00152-X DOI: https://doi.org/10.1016/S0304-4165(99)00152-X

Grzegorczyk-Karolak, I., Wysokińska, H., Olas, B. 2015. Studies on the antioxidant properties of extracts from the roots and shoots of two Scutellaria species in human blood plasma. Acta Biochimica Polonica, vol. 62, no. 2, p. 253-258. https://doi.org/10.18388/abp.2014_944 DOI: https://doi.org/10.18388/abp.2014_944

Hirunuma, M., Shoyama, Y., Sasaki, K., Sakamoto, S., Taura, F., Shoyama, Yu., Tanaka, H., Morimoto, S. 2011. Flavone-catalyzed apoptosis in Scutellaria baicalensis. Phytochemistry, vol. 72, no. 8, p. 752-760. https://doi.org/10.1016/j.phytochem.2011.02.009 DOI: https://doi.org/10.1016/j.phytochem.2011.02.009

Kavalcová, P., Bystrická, J., Tomaš, J., Karovičová, J., Kuchtová, V. 2014. Evaluation and comparison of the content of total polyphenols and antioxidant activity in onion, garlic and leek. Potravinarstvo, vol. 8, no. 1, p. 272-276. https://doi.org/10.5219/394 DOI: https://doi.org/10.5219/394

Kimura, Y., Sumiyoshi, M. 2013. Anti-tumor and anti-metastatic actions of wogonin isolated from Scutellaria baicalensis roots through anti-lymphangiogenesis. Phytomedicine, vol. 20, no. 3-4, p. 328-336. https://doi.org/10.1016/j.phymed.2012.10.016 DOI: https://doi.org/10.1016/j.phymed.2012.10.016

Kosakowska, O. 2017. Intrapopulation variability of flavonoid content in roots of Baical skullcap (Scutellaria baicalensis Georgi). Herba Polonica, vol. 63, no. 1, p. 20-31. https://doi.org/10.1515/hepo-2017-0002 DOI: https://doi.org/10.1515/hepo-2017-0002

Kouřimská, L., Ešlerová, K., Khatri, Y. 2016. The effect of storage on quality of herbs genus Origanum. Potravinarstvo, vol. 10, no. 1, p. 207-214. https://doi.org/10.5219/608 DOI: https://doi.org/10.5219/608

Leváková, L., Lacko-Bartošová, M. 2017. Phenolic acids and antioxidant activity of wheat species: a review. Agriculture (Poľnohospodarstvo), vol. 63, no. 3, p. 92-101. https://doi.org/10.1515/agri-2017-0009 DOI: https://doi.org/10.1515/agri-2017-0009

Li, W., Sun, H., Zhou, J., Zhang, Y., Liu, L., Gao, T. 2015. Antibacterial activities, antioxidant content and antioxidant properties of three traditional Chinese medicinal extracts. Bangladesh Journal of Pharmacology, vol. 10, no. 1, p. 131-137. https://doi.org/10.3329/bjp.v10i1.21324 DOI: https://doi.org/10.3329/bjp.v10i1.21324

Li, X., Wu, X., Huang, L. 2009. Correlation between antioxidant activities and phenolic contents of Radix Angelicae Sinensis (Danggui). Molecules, vol. 14, no. 12, p. 5349-5361. https://doi.org/10.3390/molecules14125349 DOI: https://doi.org/10.3390/molecules14125349

Liu, G., Rajesh, N., Wang, X., Zhang, M., Wu, K., Li, S., Chen, B., Yao, S. 2011. Identification of flavonoids in the stems and leaves of Scutellaria baicalensis Georgi. Journal of Chromatography B, vol. 879, no. 13-14, p. 1023-1028. https://doi.org/10.1016/j.jchromb.2011.02.050 DOI: https://doi.org/10.1016/j.jchromb.2011.02.050

Liu, R. X., Song, G.-H., Wu, P. G., Zhang, X.-W., Hu, H.-Y., Liu, J., Miao, X.-S., Hou, Z.-Y., Wang, W.-Q., Wei, S.-L. 2017. Distribution patterns of the contents of five biologically activate ingredients in the root of Scutellaria baicalensis. Chinese Journal of Natural Medicines, vol. 15, no. 2, p. 152-160. https://doi.org/10.1016/S1875-5364(17)30030-4 DOI: https://doi.org/10.1016/S1875-5364(17)30030-4

Marinova, G., Batchvarov, V. 2011. Evaluation of the methods for determination of the free radical scavenging activity by DPPH. Bulgarian Journal of Agricultural Science, vol. 17, no. 1, p. 11-24.

Matkowski, A., Tasarz, P., Szypuła, E. 2008. Antioxidant activity of herb extracts from five medicinal plants from Lamiaceae, subfamily Lamioideae. Journal of Medicinal Plants Research, vol. 11, no. 2, p. 321-330.

Murch, J. S., Rupasinghe Vasantha, H. P., Goodenowe, D., Saxena, K. P. 2004. A metabolomic analysis of medicinal diversity in Huang-qin (Scutellaria baicalensis Georgi) genotypes: discovery of novel compounds. Physiology and Biochemistry, vol. 23, no. 6, p. 419-425. https://doi.org/10.1007/s00299-004-0862-3 DOI: https://doi.org/10.1007/s00299-004-0862-3

Ohigashi, H., Takagaki, T., Koshimizu, K., Watanabe, K., Kaji, M., Hoshino, J., Nishida, T., Huffman, M. A., Takasaki, H., Jato, J., Muanza, N. D. 1991. Biological activities of plant extracts from tropical Africa. African Study Monographs, vol. 12, no. 4, p. 201-210.

Olennikov, D. N., Chirikova, N. K., Tankhaeva, L. M. 2010. Phenolic compounds of Scutellaria baicalensis Georgi. Russian Journal of Bioorganic Chemistry, vol. 36, no. 7, p. 816-824. https://doi.org/10.1134/S1068162010070046 DOI: https://doi.org/10.1134/S1068162010070046

Prieto, P., Pineda, M., Aguilar, M. 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry, vol. 269, no. 2, p. 337-241. https://doi.org/10.1006/abio.1999.4019 DOI: https://doi.org/10.1006/abio.1999.4019

Robbins, R. J. 2003. Phenolic acids in food: an overview of analytical methodology. Food Chemistry, vol. 51, no. 10, p. 2866-2887. https://doi.org/10.1021/jf026182t DOI: https://doi.org/10.1021/jf026182t

Sánchéz-Moreno, C., Larrauri, A., Saura-Calixto, F. 1998. A procedure to measure the antioxidant efficiency of polyphenols. Journal of the Science of Food and Agriculture, vol. 76, no. 2, p. 270-276. https://doi.org/10.1002/(SICI)1097-0010(199802)76:2<270::AID-JSFA945>3.0.CO;2-9 DOI: https://doi.org/10.1002/(SICI)1097-0010(199802)76:2<270::AID-JSFA945>3.0.CO;2-9

Saxena, M., Saxena, J., Pradhan, A. 2012. Flavonoids and phenolic acids as antioxidants in plants and human health. International Journal of Pharmaceutical Sciences Review and Research, vol. 16, no. 2, p. 130-134.

Seo, N. O., Kim, G.-S., Kim, Y.-H., Rark, S., Jeong, S. W., Lee, S. J., Jin, J. S., Shin, S. C. 2013. Determination of polyphenol components of Korean Scutellaria baicalensis Georgi using liquid chromatography–tandem mass spectrometry: contribution to overall antioxidant activity. Journal of Functional Foods, vol. 5, no. 4, p. 1741-1750. https://doi.org/10.1016/j.jff.2013.07.020 DOI: https://doi.org/10.1016/j.jff.2013.07.020

Shafii, Z. A., Basri, M., Malek, E. A., Ismail, M. 2017. Phytochemical and antioxidant properties of Manilkara zapota (L.) P roen fruit extracts and its formulations for cosmceuetical application. Asian Journal of Plant Science and Research, vol. 7, no. 3, p. 29-41.

Singleton, V. L., Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagent. American Journal of Enology and Agricultural, vol. 6, p. 144-158.

Stehlíková, B. 1998. Basics of biostatiscics (Biodiversity protection 51). Nitra, Slovakia : SPU, 79 p. ISBN 80-7137-539-X.

Tatiya, U. A., Tapadiya, G. G., Kotecha, S., Surana, J. S. 2011. Effect of solvents on total phenolics, antioxidant and antimicrobial properties of Bridelia retusa Spreng. stem bark. Indian Journal of Natural Products and Resources, vol. 2, no. 4, p. 442-447.

Vamanu, E., Vamanu, A., Nita, S., Colceriu, S. 2011. Antioxidant and antimicrobial activities of ethanol extracts of Cynara scolymus (Cynarae folium, Asteraceae family). Tropical Journal of Pharmaceutical Research, vol. 10, no. 6, p. 777-783. https://doi.org/10.4314/tjpr.v10i6.11 DOI: https://doi.org/10.4314/tjpr.v10i6.11

Wang, Y. F., Xu, Y. L., Tang, Z. H., Li, T., Zhang, L. L., Chen, X., Lu, J. H., Leung, C. H., Ma, D. L., Qiang, W. A., Wang, Y. T., Lu, J. J. 2017. Baicalein induces beclin 1- and extracellular signal-regulated kinase-dependent autophagy in ovarian cancer cells. American Journal of Chinese Medicine, vol. 45, no. 1, p. 123-136. https://doi.org/10.1142/S0192415X17500094 DOI: https://doi.org/10.1142/S0192415X17500094

Downloads

Published

2019-06-28

How to Cite

Vergun, O., Svydenko, L., Grygorieva, O., Shymanska, O., Rakhmetov, D., Brindza, J. ., & Ivanišová, E. . (2019). Antioxidant capacity of plant raw material of Scutellaria baicalensis Georgi. Potravinarstvo Slovak Journal of Food Sciences, 13(1), 614–621. https://doi.org/10.5219/1090

Issue

Vol. 13 No. 1 (2019): Potravinarstvo Slovak Journal of Food Sciences

Section

Articles

License

This license permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

Crossref
Scopus
Google Scholar
Europe PMC

Most read articles by the same author(s)

<< < 1 2 3 4 5 6 > >> 

Similar Articles

You may also start an advanced similarity search for this article.