Occurence and antimicrobial resistance of common udder pathogens isolated from sheep milk in Slovakia


  • Ivan Holko VETSERVIS, s.r.o. Kalvária 3, 94901 Nitra, Slovakia https://orcid.org/0000-0002-8273-9241
  • Vladimí­r Tančin Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of veterinary science, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia; NPPC-Research Institute for Animal Production Nitra, Hlohovecká 2, 95141 Lužianky, Slovakia https://orcid.org/0000-0003-2908-9937
  • Kristí­na Tvarožková Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Veterinary Disciplines, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia https://orcid.org/0000-0003-4989-6138
  • Peter Supuka VETSERVIS, s.r.o., Kalvária 3, 94901 Nitra, Slovakia
  • Anna Supuková VETSERVIS, s.r.o., Kalvária 3, 94901 Nitra, Slovakia
  • Mačuhová Lucia NPPC-Research Institute for Animal Production Nitra, Hlohovecká 2, 95141 Lužianky, Slovakia https://orcid.org/0000-0002-9624-1348




sheep, milk, mastitis, pathogen, antibiotic


The aim of this work is to identify the spectrum, frequency and antimicrobial resistance of bacterial pathogens occurring in sheep dairy herds in Slovakia. Of a total of 310 samples of sheep's milk coming from three breeds during two seasons (2017 and 2018), at least one potential pathogen was isolated from 102 samples (32.9%). A total of 131 microbial isolates were isolated. The most represented species were coagulase negative staphylococci CoNS (75.6%), followed by Streptococcus agalactiae (10.7%), Staphylococcus aureus (6.9%), Streptococcus dysgalactiae (4.6%), Escherichia coli (1.5%), Enterococcus faecium (1.5%), and others (Streptococcus uberis, Streptococcus parauberis, Candida sp., Klebsiella sp., moulds) below 1%. A total of 99 isolates of CoNS were tested for antimicrobial resistance. Of these, 63.6% were resistant to at least one antibiotic. A total of 24.2% of the tested isolates were resistant to 3 groups of antimicrobials simultaneously (multi-drug resistance). The highest resistance was observed to lincomycin (57.6%) and neomycin (36.4%), the lowest to sulfamethoxazolum+trimethoprim (0%) and enrofloxacin (3.0%). Based on the results of this work, it is possible to assume a similar spectrum of pathogens and their antimicrobial resistance described in the literature also within the Slovak sheep  farms focused on milk production.


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How to Cite

Holko, I. ., Tančin, V. ., Tvarožková, K., Supuka, P., Supuková, A., & Lucia, M. (2019). Occurence and antimicrobial resistance of common udder pathogens isolated from sheep milk in Slovakia. Potravinarstvo Slovak Journal of Food Sciences, 13(1), 258–261. https://doi.org/10.5219/1067

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