Bovine mucous membranes as a source of antimicrobial compounds
DOI:
https://doi.org/10.5219/976Keywords:
AMPs, storage, shelf-life, mucous membranes, proteinsAbstract
Loss of food quality, deterioration of organoleptic properties and accumulation of anti-alimentary compounds are in focus of modern food science. Nowadays, such traditional methods as processing, physical and chemical treatment are used for improving of shelf life. An alternative ways of shelf life increasing are quite a sharp problem. Antimicrobial peptides (AMPs) could be an actual alternative. According to Antimicrobial Peptide Database (http://aps.unmc.edu/AP/main.php), 2884 antimicrobial peptides from six kingdoms were found and identified. Mucous membranes of farm animals due to their border position and intensive contact with different pathogens could be a capacious source of such substances. Objects of the study were bovine oral cavity mucosa, nasal cavity mucosa, tracheal cavity mucosa, rectal mucosa, tongue mucosa, saliva gland and submandibular lymph nodes. Two-dimensional electrophoresis (2DE) was performed according to the method of O'Farrell, 35 protein fractions were identified by MALDI-TOF MS and MS/MS mass spectrometry. A number of qualitative and quantitative differences were revealed. A large number of histones (H2bd-like, H2BC, HIST1H2BD, HIST2H2AC, HIST1H2AH, histone H3.3 and H2bl-like, HIST2H2AC and histone H3.3, mixture HIST1H2AJ, HIST2H2BE and histone H2A type 2-C) were found in all mucous membranes as well as several tissue-specific proteins (proteins S100-A12 and AGR2, isoforms of ribosomal proteins, myelin P2, odorant-binding protein, secretoglobin), which could be a precusors of bioactive peptides
Downloads
Metrics
References
Bahar, A. A., Ren, D. 2013. Antimicrobial peptides. Pharmaceuticals (Basel), vol. 6, no. 12, p. 1543-1575. https://doi.org/10.3390/ph6121543 DOI: https://doi.org/10.3390/ph6121543
Bilek, M., Vietoris, V., Ilko, V. (2016) Shelf life extension and sensory evaluation of birch tree sap using chemical preservatives. Potravinarstvo, vol. 10, no. 1, p. 499-505. https://doi.org/10.5219/649 DOI: https://doi.org/10.5219/649
Cole, A. M., Kim, Y. H., Tahk, S., Hong, T., Weis, P., Ganz, T. 2001. Calcitermin, a novel antimicrobial peptide isolated from human airway secretions. FEBS Letters, vol. 504, no. 1-2, p. 5-10. https://doi.org/10.1016/S0014-5793(01)02731-4 DOI: https://doi.org/10.1016/S0014-5793(01)02731-4
Dikeman, M., Devine, C. 2014. Encyclopedia of Meat Sciences. 2nd ed. London, UK : Academic Press, 1712 p. ISBN 978-0-12-384734-8.
Jarczak, J., Kościuczuk, E. M., Lisowski, P., Strzałkowska, N., Jóźwik, A., Horbańczuk, J., Krzyżewski, J., Zwierzchowski, L., Bagnicka, E. 2013. Defensins: Natural component of human innate immunity. Human Immunology, vol. 74, no. 9, p. 1069-1079. https://doi.org/10.1016/j.humimm.2013.05.008 DOI: https://doi.org/10.1016/j.humimm.2013.05.008
Kameník, J. 2013. The microbiology of meat spoilage: a review. Maso International - Journal of Food Science and Technology, vol. 2013, no. 1, p. 1-9.
Kovalyov, L. I., Kovalyova, M. A., Kovalyov, P. L., Serebryakova, M. V., Moshkovskii, S. A., Shishkin, S. S. 2006. Polymorphism of delta3,5-delta2,4-dienoyl-coenzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue. Biochemistry (Moscow), vol. 71. no. 4, p. 448-453. https://doi.org/10.1134/S0006297906040146 DOI: https://doi.org/10.1134/S0006297906040146
Nesterenko, A. A., Kayatskaya A. S. 2012. Pickles of meat and meat products. Vestnik NGIEI, no. 8, p. 46-54.
Popelka, P., Jevinová, P., Marcinčák, S. 2016. Microbiological and chemical quality of fresh and frozen whole trout and trout fillets. Potravinarstvo, vol. 10, no. 1, p. 431-436. https://doi.org/10.5219/599 DOI: https://doi.org/10.5219/599
Shamova, O. V., Orlov, D. S., Balandin, S. V., Shramova, E. I., Tsvetkova, E. V., Panteleev, P. V., Leonova, Yu, F., Tagaev, A. A., Kokryakov, V. N., Ovchinnikova, T. V. 2014. Acipensins - novel antimicrobial peptides from leukocytes of the Russian sturgeon Acipenser gueldenstaedtii. Acta Naturae, vol. 6, no. 4, p. 99-109. DOI: https://doi.org/10.32607/20758251-2014-6-4-99-109
Syasin, I. E. 2011. Features of cryopreservation and cryoseparation of food raw materials. Polythematic online scientific journal of Kuban State Agrarian University, vol. 66, no 2, p. 1-12.
Tagai, C., Morita, S., Shiraishi, T., Miyaji, K., Iwamuro, S. 2011. Antimicrobial properties of arginine- and lysine-rich histones and involvement of bacterial outer membrane protease T in their differential mode of actions. Peptides, vol. 32, no. 10, p. 2003-2009. https://doi.org/10.1016/j.peptides.2011.09.005 DOI: https://doi.org/10.1016/j.peptides.2011.09.005
Tecle, T., Tripathi, S., Hartshorn, K. L. 2010. Review: Defensins and cathelicidins in lung immunity. Innate immunity, vol. 16, no. 3, p. 151-159. https://doi.org/10.1177/1753425910365734 DOI: https://doi.org/10.1177/1753425910365734
The Antimicrobial Peptide Database. Available at: http://aps.unmc.edu/AP/main.php.
Tuniyeva, E. K. 2013. Ingredients and packaging at IFFA 2013: the easy consumption of natural products. Vse o myase, vol. 2013, no. 3, p. 5-7.
Tuniyeva, E. K. 2015. To a safety issue of food additives. Vse o myase, vol. 2015, no. 4, p. 10-13.
Wang, G. 2010. Antimicrobial Peptides: Discovery, Design and Novel Therapeutic Strategies. Oxfordshire, UK: CABI. 240 p. ISBN-13: 978-1845936570. https://doi.org/10.1079/9781845936570.0000 DOI: https://doi.org/10.1079/9781845936570.0000
Wang, G. 2015. Improved methods for classification, prediction, and design of antimicrobial peptides. Methods in molecular biology, vol. 1268, p. 43-66. https://doi.org/10.1007/978-1-4939-2285-7_3 DOI: https://doi.org/10.1007/978-1-4939-2285-7_3
Zaitseva, U. A., Girina, E. G., Ponomarenko, A. V. 2014. Types of pickles and its application in the meat industry. Molodoi ychenyi, vol. 63, no. 4, p. 164-167.
Zhao, L., Lu, W. 2014. Defensins in innate immunity. Current Opinion in Hematology, vol. 21, no. 1, p. 37-42. https://doi.org/10.1097/MOH.0000000000000005 DOI: https://doi.org/10.1097/MOH.0000000000000005
Zolotokopova, S. V., Palagina, I. A. 2007. Theoretical study of preservative action mechanism of smoke components in the extracts. Izvestia vuzov. Pishevaya tekhnologia, vol. 298, no. 3, p. 36-42.
Zvereva, E. A., Kovalev, L. I., Ivanov, A. V., Kovaleva, M. A., Zherdev, A.V., Shishkin, S. S., Lisitsyn, A. B., Chernukha, I. M., Dzantiev, B. B. 2015. Enzyme immunoassay and proteomic character-ization of troponin I as a marker of mammalian muscle compounds in raw meat and some meat products. Meat Science, vol. 105, p. 46-52. https://doi.org/10.1016/j.meatsci.2015.03.001 DOI: https://doi.org/10.1016/j.meatsci.2015.03.001
Downloads
Published
How to Cite
Issue
Section
License
This license permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
