Effect of humates in diet of dairy cows on the raw milk main components
DOI:
https://doi.org/10.5219/748Keywords:
humic substances, Sakhalin Leonardite, dairy cows, milk proteins, caseinAbstract
The effect of supplemental humic substances (HS) on the main milk components was investigated. A total of 10 dairy cows (Czech pied cattle, crossbred Czech pied cattle ´ Ayrshire and crossbred Czech pied cattle ´ Red Holstein) were tested. Animals were randomly divided into 2 groups, control (C) and experimental (E). Animals fed the same feed mixture and group E was additionally supplemented with HS (200 mg.kg-1 of product Humafit prepared from the Sakhalin Leonardite). The experimental period took 3 months. Cows were milked twice a day. The milk composition (lactose, fat, crude protein, pure protein and casein) of every cow was monitored on days 0, 14, 28, 42, 56, 70 and 84 of the experiment. Pure protein content was determined by Kjeldahl method, other components were analysed using an infrared analyserMilkoScan FT 120. It was found that the crude protein, pure protein and casein content in milk of group E significantly (p <0.05) increased from the 56th day of the experimental period. Differences of the protein fraction contents in group C and of the dry matter, non-fat dry matter, lactose and fat content in both groups were non-significant (p <0.05).Higher protein and especially casein content in milk could be very important for the cheesemaking as it could increase the cheese yield.
Downloads
Metrics
References
Agazzi, A., Cigalino, G., Mancin, G., Savoini, G., Dell'Orto, V. 2007. Effects of dietary humates on growth and an aspect of cell-mediated immune response in newborn kids. Small Ruminant Research, vol. 72, no. 2-3, p. 242-245. https://doi.org/10.1016/j.smallrumres.2006.10.020 DOI: https://doi.org/10.1016/j.smallrumres.2006.10.020
AOAC. 2012. Official methods of analysis. 19th ed. Arlington, USA: Association of Official Analytical Chemists.
Avci, M., Denek, N., Kaplan, O. 2007. Effects of humic acid at different levels on growth performance, carcass yields and some biochemical parameters of quails. Journal of Animal and Veterinary Advances, vol. 6, no. 1, p. 1-4.
Bai, H. X., Chang, Q. F., Shi, B. M., Shan, A. S. 2013. Effects of fulvic acid on growth performance and meat quality in growing-finishing pigs. Livestock Science, vol. 158, no. 1-3, p. 118-123. https://doi.org/10.1016/j.livsci.2013.10.013 DOI: https://doi.org/10.1016/j.livsci.2013.10.013
Bujko, J., Kocman, R., Žitný, J., Trakovická, A., Hrncar, C 2011. Analyse of traits of milk production in dairy cows. Potravinarstvo, vol. 5, no. 1, p. 5-9. https://doi.org/10.5219/93 DOI: https://doi.org/10.5219/93
CMDA 2013. Statistic of milk purchasing and processing (Statistika nákupu a zpracování mléka), Czech and Moravian Dairy Association [online] s.a. [cit. 2013-01-25] Available at: http://www.cmsm.cz/statistika.
Cunha, G. D., Romão, L. P. C., Macedo, Z. S. 2014. Production of alpha-alumina nanoparticles using aquatic humic substances. Powder Technology, vol. 254, p. 344-351. https://doi.org/10.1016/j.powtec.2014.01.008 DOI: https://doi.org/10.1016/j.powtec.2014.01.008
Degirmencioglu, T. 2012. Using humic acid in diets for Saanen goats. Mljekarstvo, vol. 62, no. 4, p. 278-283. DOI: https://doi.org/10.3923/rjnasci.2012.4.7
Degirmencioglu, T. 2014. Using humic acid in diets for dairy goats. Animal Science Papers and Reports, vol. 32, no. 1, p. 25-32.
FASS. 2010. Guide for the Care and Use of Agricultural Animals in Research and Teaching. 3rd ed. Champaign, USA: The Federation of Animal Science Societies.
Filipejová, T., Kováčik, J., Kirchnerová, K., Foltýs, V. 2011. Changes in milk composition as a result of metabolic disorders of dairy cows. Potravinarstvo, vol. 5, no. 1, p. 10-16. https://doi.org/10.5219/113 DOI: https://doi.org/10.5219/113
Gładkowski, W., Kiełbowicz, G., Chojnacka, A., Gil, M., Trziszka, T., Dobrzański, Z., Wawrzeńczyk, C. 2011. Fatty acid composition of egg yolk phospholipid fractions following feed supplementation of Lohmann Brown hens with humic-fat preparations. Food Chemistry, vol. 126, no. 3, p. 1013-1018. https://doi.org/10.1016/j.foodchem.2010.11.112 DOI: https://doi.org/10.1016/j.foodchem.2010.11.112
Hayirli, A., Esenbuğa, N., Macit, M., Laçin, E., Karaoğlu, M., Karaca, H., Yildiz, L. 2005. Nutrition practice to alleviate the adverse effects of stress on laying performance, metabolic profile, and egg quality in peak producing hens: I. The humate supplementation. Asian-Australasian Journal of Animal Sciences, vol. 18, no. 9, p. 1310-1319. https://doi.org/10.5713/ajas.2005.1310 DOI: https://doi.org/10.5713/ajas.2005.1310
Islam, K. M. S., Schuhmacher, A., Gropp, J. M. 2005. Humic acid substances in animal agriculture. Pakistan Journal of Nutrition, vol. 4, no. 3, p. 126-134. https://doi.org/10.3923/pjn.2005.126.134 DOI: https://doi.org/10.3923/pjn.2005.126.134
ISO 8968-1: 2014. Milk - Determination of nitrogen content - Part 1: Kjeldahl principle and crude protein calculation.
ISO 8968-3: 2004. Milk - Determination of nitrogen content - Part 3: Block-digestion method (Semi-micro rapid routine method).
ISO 8968-5.: 2001. Milk - Determination of nitrogen content - Part 5: Determination of protein-nitrogen content.
ISO 9622: 2013. Milk and liquid milk products - Guidelines for the application of mid-infrared spectrometry.
Kouřimská, L., Legarová, V., Panovská, Z., Pánek, J. 2014. Quality of cows' milk from organic and conventional farming. Czech Journal of Food Sciences, vol. 32, no. 4, p. 398-405. DOI: https://doi.org/10.17221/510/2012-CJFS
Kucukersan, S., Kucukersan, K., Colpan, I., Goncuoglu, E., Reisli, Z., Yesilbag, D. 2005. The effects of humic acid on egg production and egg traits of laying hen. Veterinární Medicína - Czech, vol. 50, no. 9, p. 406-410. DOI: https://doi.org/10.17221/5640-VETMED
Lange, N., Kuhnert, M., Haase, A., Hoke, H., Seubert, B. 1996. Studies concerning the resorption properties of a low molecular humic substance after single oral application to rats. Deutsche Tierarztliche Wochenschrift, vol. 103, no. 4, p. 134-135.
Madronová, L. 2011. Humic acids from raw materials of the Czech Republic. 1st ed. New York, USA : Nova Science Publishers.
NRC. 2001. Nutrient Requirements of Dairy Cattle. 7th ed. Washington, USA : The National Academies Press, National Research Council.
Novák, F., Hrabal, R. 2011. Quantitative C-13 NMR spectroscopy of humic acids. Chemicke Listy, vol. 105, no. 10, p. 752-760.
Písaříková, B., Zralý, Z., Herzig, I. 2010. The effect of dietary sodium humate supplementation on nutrient digestibility in growing pigs. Acta Veterinaria Brno, vol. 79, no. 3, p. 349-353. https://doi.org/10.2754/avb201079030349 DOI: https://doi.org/10.2754/avb201079030349
Rose, M. T., Patti, A. F., Little, K. R., Brown, A. L., Jackson, W. R., Cavagnaro, T. R. 2014. A meta-analysis and review of plant-growth response to humic substances: Practical implications for agriculture. Advances in Agronomy, vol. 124, p. 37-89. https://doi.org/10.1016/B978-0-12-800138-7.00002-4 DOI: https://doi.org/10.1016/B978-0-12-800138-7.00002-4
Stevenson, F. J. 1994. Humus chemistry: Genesis, Composition, Reactions. 2nd ed. New York, USA : John Wiley & Sons.
Šamudovská, A., Demeterová, M. 2010. Effect of diet supplemented with natural humic compounds and sodium humate on performance and selected metabolic variables in broiler chickens. Acta Veterinaria Brno, vol. 79, no. 3, p. 385-393. https://doi.org/10.2754/avb201079030385 DOI: https://doi.org/10.2754/avb201079030385
Trckova, M., Matlova, L., Hudcova, H., Faldyna, M., Zraly, Z., Dvorska, L., Beran, V., Pavlik, I. 2005. Peat as a feed supplement for animals: a review. Veterinární Medicína - Czech, vol. 50, no. 8, p. 361-377. DOI: https://doi.org/10.17221/5635-VETMED
Visser, S. A. 1973. Some biological effects of humic acids in the rat. Acta Biologica et Medica Germanica, vol. 31, no. 4, p. 569-581. PMid:4781905
Wang, Q., Chen, Y. J., Yoo, J. S., Kim, H. J., Cho, J. H., Kim, I. H. 2008. Effects of supplemental humic substances on growth performance, blood characteristics and meat quality in finishing pigs. Livestock Science, vol. 117, no. 2-3, p. 270-274. https://doi.org/10.1016/j.livsci.2007.12.024 DOI: https://doi.org/10.1016/j.livsci.2007.12.024
Zajác, P., Tomáška, M., Murárová, A., Čapla, J., Čurlej, J. 2012. Quality and safety of raw milk in Slovakia in 2011. Potravinarstvo, vol. 6, no. 2, 2012, p. 64-73. https://doi.org/10.5219/189 DOI: https://doi.org/10.5219/189
Zajác, P., Čapla, J., Vietoris, V., Zubrická, S., Čurlej, J. 2015. Effects of storage on the major constituents of raw milk. Potravinarstvo, vol. 9, no. 1, 2015, p. 375-381. https://doi.org/10.5219/518 DOI: https://doi.org/10.5219/518
Downloads
Published
How to Cite
Issue
Section
License
This license permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.