Changes in the microflora composition of goat and sheep milk during lactation
DOI:
https://doi.org/10.5219/489Keywords:
goat milk, sheep milk, microorganisms, total count of microorganismsAbstract
The aim of this work was to determine extend of microbial contamination of raw milk in individual seasons. Raw goat milk (3 farms) and sheep milk (2 farms) were analyzed. Milk was produced on farms of different way of farming and with a different number of milked animals. Samples were taken during lactation three terms in the beginning, middle and end of lactation. In milk, following groups of microorganisms were determined by standard methods: total count of microorganisms (TCM), psychrotrophic microorganisms, Enterobacteriaceae, lactic acid bacteria (lactobacilli), enterococci, aerobic and anaerobic thermoresistant microorganisms (TMRae, TMRan), micromycetes (yeast and moulds). In goat milk, the following numbers of microorganisms were detected: total count of microorganisms (TCM) from 105 to 109 CFU x mL-1, lactobacilli from 102 to 105 CFU x mL-1, bacteria fam. Enterobacteriaceae from 101 to 105 CFU x mL-1, enterococci from 101 to 105 CFU x mL-1, thermoresistant aerobic and anaerobic microorganisms (TMRae and TMRan) from units to 103 resp. 105 CFU x mL-1, psychrotrophic microorganisms from 101 to 106 CFU x mL-1, micromycets from 101 to 104 CFU x mL-1. In the sheep milk, the following numbers of microorganisms were determined: TCM from 105 to 106 CFU x mL-1, lactobacilli from 103 to 106 CFU x mL-1, bacteria fam. Enterobacteriaceae from 101 to 105 CFU x mL-1, enterococci from 101 to 104 CFU x mL-1, TMRae and TMRan from units to 105 CFU x mL-1, psychrotrophic microorganisms from 104 to 106 CFU x mL-1, micromycets from 102 to 104 CFU x mL-1. From the above mentioned results, the following conclusions can be suggested. The bacterial counts of raw goat and sheep milk are highly variable and influenced by a number of important factors in the course of lactation and year (temperature, health, secondary contamination etc.). The bacterial numbers are not affected by the stage of lactation. High numbers of microorganisms in goat and sheep milk may be primarily caused by the insufficient cleaning and sanitizing of milking equipment or low hygiene of hand milking. An important role may also act the cooling rate of milk and purity of cooling eguipment.
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Cempírková, R., Samková, E., Vyletělová, M. 2012. Celkový počet mikroorganismů (The total count of microorganisms). In Vyletělová, E. (edit.): Mléko: produkce a kvalita, Jihočeská univerzita v Českých Budějovicích, p. 122-127. ISBN 978-80-7394-383-7.
ČSN 570529:1993. Syrové kravské mléko pro mlékárenské ošetření a zpracování (Raw cow milk for dairy processing).
Foschino, R., Invernizzi, A., Barucco, R., Stradiotto, K. 2002. Microbial composition, including the incidence of pathogens, of goat milk from the Bergamo region of Italy during a lactation year. J. of Dairy Research, vol. 69, no. 2, p. 213-225. https://doi.org/10.1017/S0022029902005459 DOI: https://doi.org/10.1017/S0022029902005459
Görner, F., Valík, Ľ. 2004. Aplikovaná mikrobiológia poživatin (Applied food microbiology). Malé Centrum, Bratislava, p. 171-195. ISBN 80-967064-9-7.
Hejlová, Š. 1997. Mikrobiologie mléka a mléčných výrobků (Microbiology of milk and milk products).Ve: Cempírková, R., Lukášová, J., Hejlová, Š. (edit.). Mikrobiologie potravin. Skriptum JU ZF České Budějovice, p. 99-120. ISBN 80-7040-254-7.
Kalantzopoulos, G. 2003. Kvalita ovčieho a kozieho mlieka z pohľadu IDF (The quality of sheep and goat milk, in view od IDF). Mliekarstvo, vol. 34, no. 4, p. 16-23.
Kalhotka, L., Šustová, K., Hůlová, M., Přichystalová, J. 2013. Important groups of microorganisms in raw goat milk and fresh goat cheeses determined during lactation. The Journal of Microbiology, Biotechnology and Food Sciences. vol. 2, no. 5, p. 2314-2317. ISSN 1338-5178. [cit. 2015-04-03] Available at: http://www.jmbfs.org/wp-content/uploads/2013/04
Králíčková, Š., Pokorná, M., Kuchtík, J., Filipčík, R. 2012. Effect of parity and stage of lactation on milk yield, composition and quality of organic sheep milk. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, vol. 60, no. 1, p. 71-78. https://doi.org/10.11118/actaun201260010071 DOI: https://doi.org/10.11118/actaun201260010071
Malá, G., Švejcarová, M., Knížek, J., Peroutková, J. 2010. Je mikrobiologická kvalita ovčího mléka ovlivněna způsobem dojení? (Is the microbiological quality of sheep milk influenced by the way of the milking?). Farmářská výroba sýrů a kysaných mléčných výrobků VII. Mendelova univerzita v Brně, p. 37-38. ISBN 978-80-7375-402-0.
Morgan. F., Massouras, T., Barbosa, M., Roserio, L., Ravasco, F., Kandarakis, I., Bonnin, V., Fistakoris, M., Anifantakis, E., Jaubert, G., Raynal-Ljutovac, K. 2003. Characteristics of goat milk collected from small and medium enterprises in Greece, Portugal and France. Small Ruminant Research, vol. 47, p. 39-49. https://doi.org/10.1016/S0921-4488%2802%2900252-3 DOI: https://doi.org/10.1016/S0921-4488(02)00252-3
Oliveira, C. J. B., Hirsch, E. R., Moura, J. F. P., Givisiez, P. E. N., Costa, R. G., Gebreyes, W. A. 2011. On farm risk factors associated with goat milk quality in Northeast Brazil. Small Ruminant Research, vol. 98, p. 64-69. https://doi.org/10.1016/j.smallrumres.2011.03.020 DOI: https://doi.org/10.1016/j.smallrumres.2011.03.020
Regulation (EC) No 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal.
Švejcarová, M., Elich, O., Pechačová, M., Zenaib, A., Malá, G. 2010. Vliv způsobu dojení a přípravy mléčné žlázy na mikrobiologické a chemické parametry dojných ovcí (The effect of milking way and treatment of lacteal gland and on microbiological and chemical parameters of sheep milk). Mlékařské listy - Zpravodaj 123, p. 21-24. ISSN 1212-950X. [cit. 2015-04-03] Available at: http://www.mlekarskelisty.cz/upload/soubory/pdf/2010/123_s._xxi-xxiv.pdf
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