Quality of meat of rabbits after application of epicatechin and patulin

Authors

  • Anna Kalafová Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 Nitra
  • Jozef Bulla Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 Nitra
  • Ondrej Bučko Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Department of Animal Husbandry, Trieda A. Hlinku 2, 949 76 Nitra
  • Jana Emrichová Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 Nitra
  • Katarí­na Zbyňovská Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 Nitra
  • Peter Petruška Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 Nitra
  • Monika Schneidgenová Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 Nitra
  • Ľubica Chrastinová National Agricultural and Food Centre, Research Institute of Animal Production, Hlohovecka 2, 949 01 Nitra
  • Ľubomí­r Ondruška National Agricultural and Food Centre, Research Institute of Animal Production, Hlohovecka 2, 949 01 Nitra
  • Rastislav Jurčí­k National Agricultural and Food Centre, Research Institute of Animal Production, Hlohovecka 2, 949 01 Nitra
  • Martin Mellen Hydina Slovensko (Poultry Slovakia), 065 11 Nova Lubovna
  • Marcela Capcarová Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/450

Keywords:

Rabbits, epicatechin, patulin, pH level, meat quality

Abstract

The aim of the present study was to determinate the effect of epicatechin and patulin on selected parameters of meat quality of rabbits. Adult female rabbits (n=25), maternal albinotic line (crossbreed Newzealand white, Buskat rabbit, French silver) and paternal acromalictic line (crossbreed Nitra's rabbit, Californian rabbit, Big light silver) were used in experiment. Animals were divided into five groups: control group (C) and experimental groups E1, E2, E3, and E4. Animals from experimental groups E1, E2, E3, E4 received patulin through intramuscular injection (10 µg.kg-1) twice a week and animals from groups E2, E3, E4 received epicatechin three times a week through intramuscular injection. After 30 days animals were slaughtered. For analysing of meat quality the samples of Musculus longissimus dorsi (50 g) were used. Application of  epicatechin and patulin to rabbits had slight or no effect on the pH levels in stomach, small intestine, large intestine and urinary bladder contents, however differences among the groups were insignificant (p ˃0.05). Application of epicatechin and patulin to rabbits had slight or no effect on total water, protein, fat   and differences among the groups were insignificant (p >0.05). The values of amino acids concentrations were not influenced after application of epicatechin and patulin. The fatty acid profiles in animals after application of different doses of epicatechin and 10 µg.kg-1 patulin were similar (p >0.05). Concentration of cholesterol increased in experimental groups in comparison with the control group, but differences were insignificant (p >0.05). pH levels of meat of rabbits in experimental group E3 was lower when compared with the control group, but differences was not significant (p >0.05).  Electric conductivity parameter was increased in each experimental group (in E3 the highest) against the control but without significant differences (p >0.05). Colour L parameter was slightly decreased in experimental groups with comparison to the control group (in E3 the lowest). Generally we can conclude that intramuscular application of epicatechin or patulin did not affect parameters of meat quality as well as pH values of internal organs content. Further investigations are needed to prove the final answer concerning the health promoting effects of epicatechin and patulin.

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References

Al-Gayyar, M. M., Matragoon, S., Pillai, B. A., Ali, T. K., Abdelsaid, M. A., El-Remessy, A. B. 2011. Epicatechin blocks pro-nerve growth factor (proNGF)-mediated retinal neurodegeneration via inhibition of p75 neurotrophin receptor expression in a rat model of diabetes. Diabetologia, vol. 54, no. 3, p. 669-680. https://doi.org/10.1007/s00125-010-1994-3 PMid:21136036 DOI: https://doi.org/10.1007/s00125-010-1994-3

Alves, I., Oliveira, N. G., Laires, A., Rodrigues, A. S. 2000. Induction of micronuclei and chromosomal aberrations by the mycotoxin patulin in mammalian cells: role of ascorbic acid as a modulator of patulin clastogenicity. Mutagenesis, vol. 15, p. 229-234. https://doi.org/10.1093/mutage/15.3.229 PMid:10792015 DOI: https://doi.org/10.1093/mutage/15.3.229

Arafat, W., Kern, D., Dirheimer, G. 1985. Inhibition of aminoacyl-tRNA synthetases by the mycotoxin patulin. Chemico-Biological Interactions, vol. 56, no. 2-3, p. 333-49. https://doi.org/10.1016/0009-2797(85)90015-8 PMid:3907866 DOI: https://doi.org/10.1016/0009-2797(85)90015-8

Ashoor, S. H., Chu, F. S. 1973. Inhibition of muscle aldolase by penicillic acid and patulin in vitro. Food Cosmet Toxicology, vol. 11, no. 6, p. 995-1000. https://doi.org/10.1016/0015-6264(73)90226-5 PMid:4783995 DOI: https://doi.org/10.1016/0015-6264(73)90226-5

Azam, S., Hadi, N., Khan, N., Hadi, S. 2004. Prooxidant property of green tea polyphenols epicatechin and epigallocatechin-3-gallate: implications for anticancer properties. Toxicology in Vitro, vol. 18, p. 555-561. https://doi.org/10.1016/j.tiv.2003.12.012 PMid:15251172 DOI: https://doi.org/10.1016/j.tiv.2003.12.012

Bell, L. N., Labuza, T. P. 1991. Potential pH implications in the freezedried state. Journal of Cryo-Letters, vol. 12, p. 235-244.

Blasco, A., Piles, M. 1990. Muscular pH of the rabbit. Annales de Zootechnie, vol. 39, p. 133-136. https://doi.org/10.1051/animres:19900205 DOI: https://doi.org/10.1051/animres:19900205

Bodas, R., Prieto, N., Jordán, M. J., López-Campos, Ó., Giráldez, F. J., Morán, L., Andrés, S. 2012. The liver antioxidant status of fattening lambs is improved by naringin dietary supplementation at 0.15% rates but not meat quality. Animal, vol. 6, no. 5, p. 863-870. https://doi.org/10.1017/S175173111100214X PMid:22558934 DOI: https://doi.org/10.1017/S175173111100214X

Capcarová, M., Slamečka, J., Abbas, K., Kolesárová, A., Kalafová, A., Valent, M., Filipejová, T., Chrastinová, Ľ., Ondruška, Ľ., Massányi, P. 2012. Effects of dietary inclusion of Rhus coriaria on internal milieu of rabbits. Journal of Animal Physiology and Animal Nutrition, vol. 96, p. 459-465. https://doi.org/10.1111/j.1439-0396.2011.01164.x PMid:21585564 DOI: https://doi.org/10.1111/j.1439-0396.2011.01164.x

Dickens, F., Jones, H. E. H. 1961. Carcinogenic Activity of a Series of Reactive Lactones and Related Substances. British Journal of Cancer, vol. 15, no. 1, p. 85-100. PMC:2071115 DOI: https://doi.org/10.1038/bjc.1961.10

Frank, H. K., Orth, R., Figge, A. 1977. Patulin in foods of vegetable origin. 2. Several kinds of fruit and vegetables and fruit and vegetable products (author's transl). Z Lebensm Unters Forsch Journal, vol. 163, no. 2, p. 111-114. https://doi.org/10.1007/bf01126030 PMid:857490 DOI: https://doi.org/10.1007/BF01126030

Frankel, E.N. 1984. Lipid oxidation: Mechanisms, products and biological significance. Journal of the American oil Chemists' Society, vol. 61, p. 1908-1917. https://doi.org/10.1007/bf02540830 DOI: https://doi.org/10.1007/BF02540830

Fushy, D., Alt, M., Beutling, D. 2006. Quality parameters of rabbit meat. Fleischwirtschaft, vol. 86, p. 115-117.

Gandemer, G. 1999. Lipids and meat quality: lipolysis, oxidation, maillard reaction and flavour. Sciences des Aliments, vol. 19, p. 439-458.

Gómez-Guzmán, M., Jiménez, R., Sánchez, M., Romero, M., O'Valle, F., Lopez-Sepulveda, R., Quintela, A. M., Galindo, P., Zarzuelo, M. J., Bailón, E., Delpón, E., Perez-Vizcaino, F., Duarte, J. 2011. Chronic (-)-epicatechin improves vascular oxidative and inflammatory status but not hypertension in chronic nitric oxide-deficient rats. British Journal of Nutrition, vol. 106, no. 9, p. 1337-1348. https://doi.org/10.1017/s0007114511004314 PMid:21910946 DOI: https://doi.org/10.1017/S0007114511004314

Hammerstone, J. F., Lazarus, S. A., Schmitz, H. H. 2000. Procyanidin content and variation in some commonly consumed foods. Journal of Nutrition, vol. 130, p. 2086-2092. [cit. 2014-27.11] Available at: http://jn.nutrition.org/content/130/8/2086S.full DOI: https://doi.org/10.1093/jn/130.8.2086S

Hernendez, P., Pla, M., Blasco, A. 1997. Relationship of meat characteristics of two lines of rabbits selected for litter size and growth rate. Journal of Animal Science, vol. 75, no. 11, p. 2936-2941. PMid:9374307 DOI: https://doi.org/10.2527/1997.75112936x

Huxley, R. R., Neil, H. A. W. 2003. The relation between dietary flavonol intake and coronary heart disease mortality: a meta-analysis of prospective cohort studies. European Journal of Clinical Nutrition, vol. 57, no. 8, p. 904. https://doi.org/10.1038/sj.ejcn.1601624 PMid:12879084 DOI: https://doi.org/10.1038/sj.ejcn.1601624

Chan, A. O., Peng, J. Z., Lam, S. K., Lai, K. C., Yuen, M. F., Cheung, H. K., Kwong, Y. L., Rashid, A., Chan, C. K., Wong, B. C. 2006. Eradication of Helicobacter pylori infection reverses E-cadherin promoter hypermethylation. Gut- BMJ Journals, vol. 55, no. 4, p. 463-468. PMid:16428266 DOI: https://doi.org/10.1136/gut.2005.077776

Chao-Zhi, Z., Wan-Gang, Z., Zhuang-Li, K., Guang-Hong, Z., Xing-Lian, Xu. 2014. Stability of an antioxidant peptide extracted from Jinhua ham. Journal of Meat Science, vol. 96, p. 783-789. https://doi.org/10.1016/j.meatsci.2013.09.004 PMid:24200571 DOI: https://doi.org/10.1016/j.meatsci.2013.09.004

Chen, L., Yang, X., Jiao, H., Zhao, B. 2002. Tea catechins protect against lead-induced cytotoxicity, lipid peroxidation, and membrane fluidity in HepG2 cells. Toxicology Sci, vol. 69, no. 1, p. 149-156. https://doi.org/10.1093/toxsci/69.1.149 PMid:12215669 DOI: https://doi.org/10.1093/toxsci/69.1.149

Chizzolini, R., Novelli, E., Zanardi, E. 1998. Oxidation in traditional Mediterranean meat products. Journal of Meat Science, vol. 49, p. 87-99. https://doi.org/10.1016/s0309-1740(98)90040-7 PMid:22060723 DOI: https://doi.org/10.1016/S0309-1740(98)90040-7

Kinjo, J., Hitoshi, M., Tsuchihashi, R., Korematsu, Y., Miyakoshi, M., Murakami, T. 2006. Hepatoprotective constituents in plants 15: protective effects of natural-occurring flavonoids and miscellaneous phenolic compounds as determined in an HepG2 cell cytotoxicity assay. Journal of Natural Medicines, vol. 60, no. 1, p. 36-41. https://doi.org/10.1007/s11418-005-0002-6 DOI: https://doi.org/10.1007/s11418-005-0002-6

Kwon, G. Y., Yoo, B. C., Koh, K. C., Cho, J. W., Park, W. S., Park, C. K. 2005. Promoter methylation of E-cadherin in hepatocellular carcinomas and dysplastic nodules. J. Korean Med. Sci., vol. 20, no. 2, p. 242-247. https://doi.org/10.3346/jkms.2005.20.2.242 PMid:15831995 DOI: https://doi.org/10.3346/jkms.2005.20.2.242

Kwon, O., Jeong, S. J., Kim, S. O., He, L., Lee, H. G., Jang, K. L., Osada, H., Jung, M., Kim, B. Y., Ahn, J. S. 2010. Modulation of E-cadherin expression by K-Ras; involvement of DNA methyltransferase-3b. J. Carcinogenesis, vol. 31, no. 7, p. 1194-1201. https://doi.org/10.1093/carcin/bgq071 PMid:20375073 DOI: https://doi.org/10.1093/carcin/bgq071

Lee, K. S., Roschenthaler, R. J. 1986. DNA-damaging activity of patulin in Escherichia coli. Applied and Environmental Microbiology, vol. 52, no. 5, p. 1046-1054. PMid:2431653 DOI: https://doi.org/10.1128/aem.52.5.1046-1054.1986

Ludewig, M.,Treel, V. N. 2003. Schlachtausbeute und Fleischqualität von Mastkaninchen in Abhängigkeit vom Alter. Fleischwirtschaft, vol. 83, p. 101-103.

Lyon, B. G., Lyon, C. E. 2002. Color of uncooked and cooked broiler leg quarters associated with chilling temperature and holding time. Poult. Sci., vol. 81, no. 12, p. 1916-1920. https://doi.org/10.1093/ps/81.12.1916 PMid:12512587 DOI: https://doi.org/10.1093/ps/81.12.1916

Mallozzi, M. A. B., Correa, B., Haraguchi, M., Neto, F. B. 1996. Effect of flavonoids on Aspergillus flavus growth and aflatoxin production. Revista de microbiologia, vol. 27, p. 161-165.

McBride, N., Hogan, S. A., Kerry, J. P. 2007. Comparative addition of rosemary extract and additives on sensory and antioxidant properties of retail packaged beef International. Journal of Food Science and Technology, vol. 42, no. 10, p. 1201-1207. https://doi.org/10.1111/j.1365-2621.2006.01342.x DOI: https://doi.org/10.1111/j.1365-2621.2006.01342.x

McDowell, L. R. 1992. Minerals in Animal and Human Nutrition. Tropical Animal Health and Production, vol. 24, no. 4, p. 241. https://doi.org/10.1007/bf02356753 DOI: https://doi.org/10.1007/BF02356753

Mohamed, R. H., Karam, R. A., Amer, M. G. 2011. Epicatechin attenuates doxorubicin-induced brain toxicity: critical role of TNFα, iNOS and NF-κB. Brain Research Bulletin, vol. 86, p. 22-28. https://doi.org/10.1016/j.brainresbull.2011.07.001 PMid:21763406 DOI: https://doi.org/10.1016/j.brainresbull.2011.07.001

Morales, H., Sanchis, V., Coromines, J., Ramos, A. J., Marín, S. 2008. Inoculum size and intraspecific interactions affects Penicillium expansum growth and patulin accumulation in apples. Food Microbiol., vol. 25, no. 2, p. 378-385. https://doi.org/10.1016/j.fm.2007.09.008 PMid:18206780 DOI: https://doi.org/10.1016/j.fm.2007.09.008

Morán, L., Andrés, S., Bodas R., Prieto, N., Giráldez, F. J. 2012a. Meat texture and antioxidant status are improved when carnosic acid is included in the diet of fattening lambs. Meat Sci., vol. 91, no. 4, p. 430-434. https://doi.org/10.1016/j.meatsci.2012.02.027 PMid:22436658 DOI: https://doi.org/10.1016/j.meatsci.2012.02.027

Morán, L., Rodríguez-Calleja, J. M., Bodas, R., Prieto, N., Giráldez, F. J., Andrés, S. 2012b. Carnosic acid dietary supplementation at 0.12% rates slows down meat discolouration in gluteus medius of fattening lambs. Journal of Meat Science, vol. 90, no. 3, p. 789-795. https://doi.org/10.1016/j.meatsci.2011.11.016 DOI: https://doi.org/10.1016/j.meatsci.2011.11.016

Morrissey, P. A., Quinn, P. B., Sheehy, P. J. A. 1994. Newer aspects of micronutrients in chronic disease: vitamin E. Proc. Nutr. Society, vol. 53, no. 3, p. 571-582. https://doi.org/10.1079/pns19940066 PMid:7886056 DOI: https://doi.org/10.1079/PNS19940066

Nieto, G., Díaz, P., Bañón, S., Garrido, M. D. 2010. Effect on lamb meat quality of including thyme (Thymus zygis ssp. gracilis) leaves in ewes' diet. Meat Sci., vol. 85, no. 1, p. 82-88. https://doi.org/10.1016/j.meatsci.2009.12.009 PMid:20374869 DOI: https://doi.org/10.1016/j.meatsci.2009.12.009

Patel, K., Borchardt, R.T. 1990. Chemical pathways of peptide degradation. Kinetics of deamidation of an asparaginyl residue in a model hexapeptide. British Journal of Pharm Research, vol. 7, no. 7, p. 703-711. PMid:2395797

Pla, M., Guerrero, L., Guardia, D., Oliver, M. A., Blasco, A. 1998. Carcass characteristics and meat quality of rabbit lines selected for different objectives: I. Between lines comparison. Livestock Production Science, vol. 54, no. 2, p. 115-123. https://doi.org/10.1016/s0301-6226(97)00179-6 DOI: https://doi.org/10.1016/S0301-6226(97)00179-6

Reed D. 1992. Interaction of vitamin E, ascorbic acid, and glutathione in protection against oxidative damage. In Vitamin E in Health and Disease; Packer L, Fuchs J. Eds.; Marcel Dekker: New York, 1992, p. 269-281.

Renaud, S., de Lorgeril, M. 1992. Wine, alcohol, platelets, and the French paradox for coronary heart disease. Lancet, vol. 339, p. 1523-1526. https://doi.org/10.1016/0140-6736(92)91277-f PMid:1351198 DOI: https://doi.org/10.1016/0140-6736(92)91277-F

Sannaveerappa, T., Westlund, S., Sandberg, A. S., Undeland, I. 2007. Changes in the antioxidative property of herrig (Clupea harengus) press juice during a simulated gastrointestinal digestion. J. Agric. Food Chem., vol. 55, no. 26, p. 10977-10985. https://doi.org/10.1021/jf0721904 PMid:18038988 DOI: https://doi.org/10.1021/jf0721904

Sanzani, S. M., De Girolano, A., Schema, L., Solfrizzo, M., Ippolito, A., Visconti, A. 2009. Control of Penicillium expansum and patulin accumulation on apples by quercetin and umbelliferone. European Food Research and Technology, vol. 228, p. 381-389. https://doi.org/10.1007/s00217-008-0944-5 DOI: https://doi.org/10.1007/s00217-008-0944-5

Scott, P. M., Fuleki, T., Harwig, J. 1977. Patulin content of juice and wine produced from moldy grapes. J. Agric. Food Chemistry, vol. 25, no. 2, p. 434-437. PMid:838989 DOI: https://doi.org/10.1021/jf60210a024

Sebranek, J. G., Bacus, J. N. 2007. Cured meat products without direct addition of nitrate or nitrite: What are the issues? Meat Sci., vol. 77, no. 1, p. 136-147. https://doi.org/10.1016/j.meatsci.2007.03.025 PMid:22061404 DOI: https://doi.org/10.1016/j.meatsci.2007.03.025

Schumacher, D. M., Metzler, M., Lehmann, L. 2005. Mutagenicity of the mycotoxin patulin in cultured Chinese hamster V79 cells, and its modulation by intracellular glutathione. Archives of Toxicology, vol. 79, no. 2, p. 110-121. https://doi.org/10.1007/s00204-004-0612-x PMid:15536543 DOI: https://doi.org/10.1007/s00204-004-0612-x

Si, H., Fu, Z., Babu, P. V. A., Zhen, W., LeRoith, T., Meaney, M. P., Voelker, K.A., Jia, Z., Grange, R. W., Liu, D. 2011. Dietary epicatechin promotes survival of obese diabetic mice and Drosophila melanogaster, J. Nutr., vol. 141,

no. 6, p. 1095-1100. https://doi.org/10.3945/jn.110.134270 PMid:21525262 DOI: https://doi.org/10.3945/jn.110.134270

Sommer, N. F., Buchanan, J. R., Fortlage, R. J. 1974. Production of patulin by Penicillium expansum. Journal of Applied Microbiology, vol. 28, no. 4, p. 589-593. DOI: https://doi.org/10.1128/am.28.4.589-593.1974

Speijers, G. J., Franken, M. A., Van Leeuwen, F. X. 1988. Subacute toxicity study of patulin in the rat: effects on the kidney and the gastro-intestinal tract. Food and Chemical Toxicology, vol. 26, no. 1, p. 23-30. https://doi.org/10.1016/0278-6915(88)90037-3 PMid:3345966 DOI: https://doi.org/10.1016/0278-6915(88)90037-3

Tang, S. Z., Kerry, J. P., Sheehan, D., Buckley, D. J., Morrissey, P. A. 2000. Dietary tea catechins and iron-induced lipid oxidation in chicken meat, liver and heart. Meat Sci., vol. 56, no. 3, p. 285-290. https://doi.org/10.1016/s0309-1740(00)00055-3 PMid:22062080 DOI: https://doi.org/10.1016/S0309-1740(00)00055-3

Toldrá, F. 1998. Proteolysis and lipolysis in flavour development of dry-cured meat products. Meat Sci., vol. 49, p. 101-110. https://doi.org/10.1016/S0309-1740(98)90041-9 PMid:22060703 DOI: https://doi.org/10.1016/S0309-1740(98)00077-1

Wang, X. Y., Li, H., Wang, H. 2007. Involvement of wine polyphenols in the protective effect against cardiovascular diseases. Proceedings of the Fifth International Symposium on Viticulture and Enology, 5, 7-77.

Williams, R. J., Spencer, J. P. E., Rice-Evans, C. 2004. Flavonoids: antioxidants or signalling molecules? Free Radic Biol. Med., vol. 36, p. 838-849. https://doi.org/10.1016/j.freeradbiomed.2004.01.001 PMid:15019969 DOI: https://doi.org/10.1016/j.freeradbiomed.2004.01.001

Wu, T. S., Liao, Y. C., Yu, F. Y., Chang, C. H., Liu, B. H. 2008. Mechanism of patulin-induced apoptosis in human leukemia cells (HL-60). Toxicol. Lett., vol. 183, p. 1-3. https://doi.org/10.1016/j.toxlet.2008.09.018 PMid:18992795 DOI: https://doi.org/10.1016/j.toxlet.2008.09.018

Yanagisawa, K., Nishio, K., Gotoh, S. 1987. Screening for carcinogens by the DNA synthesis inhibition test using human fibroblasts. Mutation Research, vol. 183, p. 89-94. https://doi.org/10.1016/0167-8817(87)90049-6 PMid:3796665 DOI: https://doi.org/10.1016/0167-8817(87)90049-6

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2015-05-14

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Kalafová, A. ., Bulla, J. ., Bučko, O. ., Emrichová, J. ., Zbyňovská, K. ., Petruška, P. ., Schneidgenová, M. ., Chrastinová, Ľubica ., Ondruška, Ľubomí­r ., Jurčí­k, R. ., Mellen, M. ., & Capcarová, M. . (2015). Quality of meat of rabbits after application of epicatechin and patulin. Potravinarstvo Slovak Journal of Food Sciences, 9(1), 124–131. https://doi.org/10.5219/450

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