The effect of oregano essential oil on chicken meat lipid oxidation and peroxidation


  • Mária Angelovičová Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Institute of Food Sciences, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421 037 641 5805
  • Michal Angelovič Slovak University of Agriculture, Faculty of Engineering, Department of Machines and Production Biosystems, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421037 641 4795
  • Jozef Čapla Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Institute of Food Sciences, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414371
  • Peter Zajác Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Institute of Food Sciences, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414371
  • Petra Folvarčíková Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421037 641 5805
  • Jozef Čurlej Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Institute of Food Sciences, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376415825



oregano essential oil, chicken thigh with skin, freezing, storage, oxidative


The study aimed to investigate and evaluate the oxidative stability of chicken thighs with skin stored in freezing conditions due to the effect of oregano essential oil for various times. The results were compared with a control group without the use of oregano essential oil. Samples of chicken thighs with skin were obtained from an experiment performed on a poultry farm in a deep litter breeding system. The results obtained from the application of oregano essential oil to chicken thighs with skin did not show a statistically significant difference (p >0.05) in the dry matter content, fat content and acid value compared to the control group, where coccidiostats were used in starter and growth feed mixtures. A statistically significant difference was found in the peroxide value by applying oregano essential oil to chicken thighs with skin compared to a control group containing coccidiostats in starter and growth feed mixtures when stored for 1 day at room temperature (p £0.01) and 12 months in freezing conditions at -18 °C (p £0.05) and a statistically non-significant difference (p >0.05) when thighs with skin were stored for 6 and 9 months in -18 °C freezing conditions. In the conclusion, it was stated that maintaining the oxidative stability of chicken meat means knowing the factors that affect it and prepare the conditions for its maintenance. Chicken meat is generally susceptible to oxidative damage because it is characterized by a high concentration of polyunsaturated fatty acids. With a sufficient amount of effective antioxidants, chicken meat could be a homoeostatic system, but it remains limited or free of oxidized compounds and reactive components. These questions are the subject of further research in the field of oxidative stability of chicken meat.


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Ali, S., Zhang, W., Rajpu, T, N., Khan, M. A., Li, CH., Zhou, G. 2015. Effect of multiple freeze–thaw cycles on the quality of chicken breast meat. Food Chemistry, vol. 173, p. 808-814. DOI:

Amaral, A. B., Silva, M. V., Lanes, S. C. S. 2018. Lipid oxidation in meat: mechanisms and protective factors – a review. Food Science and Technology, vol. 38, supl. 1. DOI:

Boskovic, M., Glisic, M., Djordjevic, J., Starcevic, M., Glamoclija, N., Glamoclija, N., Djordjevic, V., Baltic, M. Z. 2019. Antioxidative Activity of Thyme (Thymus vulgaris) and Oregano (Origanum vulgare) Essential Oils and Their Effect on Oxidative Stability of Minced Pork Packaged Under Vacuum and Modified Atmosphere. Journal of Food Science, vol. 84, no. 9, p. 2467-2474. DOI:

Carvalho, R. H., Ida, E. I., Madruga, M. S., Martínez, S. L., Shimokomaki, M., Estévez, M. 2017. Underlying connections between the redox system imbalance, protein oxidation and impaired quality traits in pale, soft and exudative (PSE) poultry meat. Food Chemistry, vol. 215, p. 129-137. DOI:

Cassens, R. G. 1994. Meat Preservation, Preventing Losses And Assuring Safety. Connecticut, USA : Food and Nutrition Press, Inc. Trumbull. 510 p. ISBN: 0917678346.

Domínguez, R., Barba, F. J., Gómez, B., Putnik, P., Bursać Kovačević, D., Pateiro, M., Santos, E. M., Lorenzo, J. M. 2018. Active packaging films with natural antioxidants to be used in meat industry: A review. Food Research International, vol. 113, p. 93-101. DOI:

Estévez, M., Morcuente, D., Ventanas, S. 2009. Determination of oxidation. In: Handbook of Muscle Foods Analysis. Ed. 1st FL, USA : CRC Press; Boca Raton. p. 221-240. DOI:

Feng, X., ¬¬Lin, C., Na, L., Wang, S., Xu, X., Zhou, G. 2017. Emulsifying properties of oxidatively stressed myofibrillar protein emulsion gels prepared with (−)-Epigallocatechin-3-gallate and NaCl. Journal of Agricultural and Food Chemistry, vol. 63, no. 13, p. 2816. DOI:

Gardini, C. H. C. 2000. Efeito da vitamina E no desempenho e na qualidade da carne de frangos de corte: dissertação. Jaboticabal (SP) : Universidade Estadual Paulista.

Ghinimi, S., Budilarto, E., Kamal-Eldin, A. 2017. The new paradigm for lipid oxidation and insights to microencapsulation of omega-3 fatty acids. Comprehensive Reviews in Food Science and Food Safety, vol. 16, p. 1206-1218. DOI:

Haščík, P., Eliman, I. O. E., Garlík, J., Kačániová, M., Bobko, M., Kňazovická, V., Vavrišinová, K., Arpášová, H., Bučko, O. 2012. Chemical composition of muscle after pollen application in nutrition of broiler chickens. Potravinarstvo, vol. 6, no. 2, p. 26-32. DOI:

Chaijan, M., Panpipat, W. 2017. Mechanism of oxidation in foods of animal origin. Natural Antioxidants. In Banerjee, R. Verma, A. K. Siddiqui, M. W.: Applications in Foods of Animal Origin. Boca Raton, USA : Apple Academic Press, Inc. 414 p. ISBN 978-1-315-36591-6. DOI:

Cheng, J. 2016. Lipid oxidation in meat. Journal of Nutrition & Food Sciences, vol. 6, p. 1-3. DOI:

Jay, J. M., Loessner, M. J., Golden, D. A. 2005. Modern Food Microbiology, 7th Ed. NY : Springer Science and Business Media. 751 p. ISBN 0387231803.

Jazi, V., Farahi, M., Khajali, F., Abousaad, S., Ferket, P., Assadisoumeh, E. 2020. Effect of dietary supplementation of whey powderand Bacillus subtilis on growth performance, gut and hepaticfunction, and muscle antioxidant capacity of Japanese quail. Journal of Animal Physiology and Animal Nutrition, vol. 104, p. 886-897. DOI:

Jump, D. B. 2002. Dietary polyunsaturated fatty acids and regulation of gene transcription. Current Opinion in Lipidology, vol. 13, p. 155-165. DOI:

Klimentová, M., Angelovičová, M. 2019. Antioxidant effect of oregano essential oil during various storage meat time of hybrid combination Ross 308. Potravinarstvo Slovak Journal of Food Sciences, vol. 13, no. 1, p. 337-343. DOI:

Klimentová, M. 2019. Sledovanie dynamiky zmien produktov oxidácie tukov mäsa v závislosti od doby skladovania a rôznej výživy výkrmových kurčiat (Investigation of the dynamics of changes in meat fat oxidation products depending on storage time and different diets of broiler chickens) : dissertation thesis. Nitra : SUA, 134 p. (In Slovak).

Králová, M. 2015. The effect of lipid oxidation on the quality of meat and meat products. Maso International Journal of Food Science and Technology, vol. 2, p. 125-132.

Leonel, F. R., Oba, A., Pelicano, E. R. L., Zeola, N. M. B. L., BoiagO, M. M., Scatolini, A. M., Lima, T. M. A., Souza, P. A.,. Souza, H. B. A. 2007. Performance, carcass yield, and qualitative characteristics of breast and leg muscles of broilers fed diets supplemented with vitamin E at different ages. Brazilian Journal of Poultry Science, vol. 9, no. 2. DOI:

Lorenzo, J. M., Gómez, M. 2012. Shelf life of fresh foal meat under MAP, over wrap and vacuum packaging conditions. Meat Science, vol. 92, p. 610-618. DOI:

Luna, A., Lábaque, M. C., Zygaglo, J. A., Marin, R. H. 2010. Effects of thymol and carvacrol feed supplementation on lipid oxidation in broiler meat. Poultry Science, vol. 89, no. 2, p. 366-370. DOI:

Marcinčák, S., Cabadaj, R., Popelka, P., Šoltýsová, L. 2008. Antioxidative effect of oregano supplemented to broilers on oxidative stability of poultry meat. Slovenian Veterinary Research, vol. 45, no. 2, p. 61-66.

Moghrovyan, A., Sahakyan, N., Babayan, A., Chichoyan, N., Petrosyan, M., Trchounian, A. 2019. Essential Oil and Ethanol Extract of Oregano (Origanum vulgare L.) from Armenian Flora as a Natural Source of Terpenes, Flavonoids and other Phytochemicals with Antiradical, Antioxidant, Metal Chelating, Tyrosinase Inhibitory and Antibacterial Activity. Current Pharmaceutical Design, vol. 25, no. 16, p. 1809-1816. DOI:

Mohamed, A., Jamilah, B., Abbas, K. A., Rahman, R. A. 2008. A review on lipid oxidation of meat in active and modified atmosphere packaging and usage of some stabilizers. Journal of Food, Agriculture and Environment, vol. 6, p. 76-81.

Özkan, O. E., Güney, Ka., Gür, M., Pattabanoglu, E. S., Babat, E., Khalifa, M. M. 2017. Essential Oil of Oregano and Savory; Chemical Composition and Antimicrobial Activity. Indian Journal of Pharmaceutical Education and Research, vol. 51, no. 3, p. S205-208. DOI:

Purriños, L., Bermúdez, R., Franco, D., Carballo, J., Lorenzo, J. M. 2011. Development of volatile compounds during the manufacture of dry-cured “Lacón,” a Spanish traditional meat product. Journal of Food Science and Technology, vol. 76, p. 89-97. DOI:

Richards, M. P. 2006. Handbook of Food Science, Technology, and Engineering. Boca Raton, USA : CRC Press; Taylor & Francis. 3632 p. ISBN 0849398487.

Ross, C. F., Smith, D. M. 2006. Use of volatiles as indicators of lipid oxidation in muscle foods. Comprehensive Reviews in Food Science and Food Safety, vol. 5, p. 18-25. DOI:

Ruban, S. W. 2009. Lipid peroxidation in muscle food: An overview. Global Veteriania, vol. 3, no. 6, p. 509-513.

Sabikun, N., Bakhsh, A., Ismail, I., Hwang, Y. H., Rahman, M. S., Joo, S. T. 2019. Changes in physicochemical characteristics and oxidative stability of pre- and post-rigor frozen chicken muscles during cold storage. Journal of Food Science and Technology, vol. 56, no. 11, p. 4809-4816. DOI:

Semjon, B., Marcinčaková, D., Koreneková, B., Bartkovsky, M., Nagy, J., Turek, P., Marcin, S. 2020. Multiple factorial analysis of physicochemical and organolepticproperties of breast and thigh meat of broilers fed a dietsupplemented with humic substances. Poultry Science, vol. 99, p. 1750-1760. DOI:

Shahidi, F., Wanasundara, U. N. 2002. Methods for measuring oxidative rancidity in fats and oils. Food lipids: Chemistry, Nutrition, and Biotechnology. NEW YORK, USA : Marcel Dekker. p. 484-507. DOI:

Sharma, M. K., Dinh, T., Adhikari, P. A. 2020. Production performance, egg quality, and small intestine histomorphology of thelaying hens supplemented with phytogenic feed additive. Journal of Applied Poultry Research, vol. 29, p. 362-371. DOI:

Shirani, V., Jazi, V., Toghyani, M., Ashayerizadeh, A., Sharifi, F., Barekatain, R. 2019. Pulicaria gnaphalodes powder in broiler diets: consequences for performance, gut health, antioxidant enzyme activity, and fatty acid profile. Poulty Science, vol. 98, p. 2577-2587. DOI:

Sierżant, K., Korzeniowska, M., Król, B., Orda, J., Wojdyło, A. 2018. Oxidative Stability of the Meat of Broilers Fed Diets Supplemented with Various Levels of Blackcurrant Extract (Ribes nigrum L.) during Different Time Period. Journal of Chemistry, vol. 2018, 9 p. DOI:

Sirri, F., Tallarico, N., MeluzzI, A., Franchini, A. 2003. Fatty acid composition and productive traits of broiler fed diets containing conjugated linoleic acid. Poultry Science, vol. 82, p. 1356-1361. DOI:

Tkačová, J., Angelovičová, M. 2013. Antioxidačná aktivita lucernovej múčky a kvalita kuracieho mäsa (Antioxidant activity of alfalfa meal and quality of chicken meat). Nitra : SUA, 99 p. ISBN 978-80-552-1116-9. (In Slovak)

Wąsowicz, E., ¬Gramza, A., Hęś, M., Jeleń, H. H., Korczak, J., Małecka, M., Szkudlarz, S. M., Rudzińska, M., amotyja, U., Wojtasiak, R. Z. 2004. Oxidation of lipids in food. Polish Journal of Food and Nutrition Sciences, vol. 54, p. 87-100.

Wood, J., Richardson, R., Nute, G., Fisher, A., Campo, M., Kasapidou, E., Sheard, P., Enser, M. 2004. Effects of fatty acids on meat quality: A review. Meat Science, vol. 66, p. 21-32. DOI:

Wood, J. D., Enser, M., Fisher, A. V., Nute, G. R., Sheard, P. R., Richardson, R. I. 2008. Fat deposition, fatty acid composition and meat quality: a review. Meat Science, vol. 78, p. 343-358. DOI:



How to Cite

Angelovičová, M., Angelovič, M., Čapla, J., Zajác, P., Folvarčíková, P., & Čurlej, J. (2021). The effect of oregano essential oil on chicken meat lipid oxidation and peroxidation. Potravinarstvo Slovak Journal of Food Sciences, 15, 1056–1068.

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