Persistence of L. monocytogenes versus adherence on solid surface
Keywords:biofilm, Listeria monocytogenes, persistence
Listeria monocytogenes is an important food-borne pathogen that frequently persists in food-processing environments despite rigorous sanitation procedures. Specific phenotypes that have been linked to persistence have been previously investigated but no clear association has been demonstrated. In this study we characterised four persistent L. monocytogenes strains isolated from food factories in Austria, Czech Republic, Denmark and Ireland in comparison with four non-persistent counterparts. The L. monocytogenes isolates were analysed for the ability to form biofilm during exponential/stationary growth phase at different temperature at low nutrition stress and adhesion to polystyrene at high-salinity conditions. Persistent and non-persistent strains did not significantly differ in the ability to form biofilm or to adhere to polystyrene microtitre plate.
BORUCKI, M. K., PEPPIN, J. D., WHITE, D., LOGE, F., AND CALL, D. R. 2003. Variation in biofilm formation among strains of Listeria monocytogenes. In Appl. Environ. Microbiol., vol. 69, 2003, p. 7336-7342.
CALY, D., TAKILT, D., LEBRET, V. AND TRESSE, O. 2009. Sodium chloride affects Listeria monocytogenes adhesion to polystyrene and stainless steel by regulating flagella expression. In Lett. Appl. Microbiol., vol. 49, 2009, p. 751-756.
CHAVANT, P., MARTINIE, B., MEYLHEUC, T., BELLON-FONTAINE, M. N., HEBRAUD, M. 2002. Listeria monocytogenes LO28: Surface physicochemical properties and ability to form biofilms at different teperatures and growth phases. In Appl. Environ. Microbiol., vol. 68, 2002, p. 728-737.
DEAN, R. B., DIXON, W. J. 1951. Simplified statistics for small numbers of observations. In Anal. Chem., vol. 23, 1951, p. 636-638.
DJORDJEVIC, D., WIEDMANN, M., MCLANDSBOROUGH, L. A. 2002. Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. In Appl. Environ. Microbiol., vol. 68, 2002, p. 2950-2958.
FARBER, J. M., PETERKIN, P. I. 1991. Listeria monocytogenes, a food-borne pathogen. In Microbiol. Rev., vol. 55, 1991, p. 476‑511.)
GRINSTEAD, D. 2009. Cleaning and sanitation in food processing environments for the prevention of biofilm formation, and biofilm removal. In Biofilms in the Food and Beverage Industries ed. Fratamico, P. M., Annous B. A. and Gunther IV, N. W. pp. 331-358. Cambridge: Woodhead Publishing.
KALMOKOFF, M. L., AUSTIN, J. W., WAN, X. D., SANDERS, G., BANERJEE, S., FARBER, J. M. 2001. Adsorption, attachment and biofilm formation among isolates of Listeria monocytogenes using model conditions. In J. Appl. Microbiol., vol. 91, 2001, p. 725-734.
KOREŇOVÁ, J., LOPAŠOVSKÁ, J., KUCHTA, T. 2009. Biofilm forming bacterial contaminants in small and medium-sized ewes' milk and meat processing enterprises in Slovakia. In J. Food Nutr. Res., vol. 48, 2009, p. 115-120.
LELIEVELD, H. L. M. 2006. Sources of contamination. In Hygiene in Food Processing ed. Lelieveld, H. L. M, Mostert, M.A. and White, B, pp. 61-75. Cambridge: Woodhead Publishing.
LIANOU, A., SOFOS, J. N. 2007. A review of the incidence and transmission of Listeria monocytogenes in ready-to-eat products in retail and food service environments. In J. Food Prot., vol. 70, 2007, p. 2172-2198.
LUNDÉN, J., AUTIO, T., KORKEALA, H. 2003. Persistent and nonpersistent Listeria monocytogenes contamination in meat and poultry processing plants. In J. Food Prot., vol. 66, 2003, p. 2062-2069.
MORETRO, T., LANGSRUD, S. 2004. Listeria monocytogenes: biofilm formation and persistence in food-processing environments. In Biofilms, vol. 1, 2004, p. 107-121.
RODRIGUES, D. A., ALMEIDA, M. A., TEIXEIRA, P. A., OLIVEIRA, R. T. AND AYEREDO, J. C. 2009. Effect of batch and fed-batch growth modes on biofilm formation by Listeria monocytogenes at different temperatures. In Curr. Microbiol., vol. 59, 2009, p. 457-462.
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