In vitro antioxidant potential and inhibitory effect of hydro-ethanolic extract from African black velvet tamarind (Dialium indium) pulp on type 2 diabetes linked enzymes

Authors

  • Olakunle Bamikole Afolabi Phytomedicine and Toxicology Laboratory, Biochemistry Unit, Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
  • Omotade Ibidun Oloyede Department of Biochemistry, Ekiti State University, P.M.B 5363, Ado”‘Ekiti
  • Abiodun Ayodele Ojo Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
  • Amos Adeyinka Onansanya Molecular and Biotechnology Laboratory, Biochemistry Unit, Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
  • Shadrach Oludare Agunbiade Nutrition and Food Science Laboratory, Biochemistry Unit, Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
  • Bashir Olaitan Ajiboye Phytomedicine and Toxicology Laboratory, Biochemistry Unit, Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
  • Jonathan Johnson Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
  • Omolara Abosede Peters Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State

DOI:

https://doi.org/10.5219/911

Keywords:

Type 2 Diabetes, α-amylase, Dialium indium, α-glucosidase, antioxidant

Abstract

The alarming rate of diabetes mellitus (DM) globally is bothersome and has drawn the search light of researchers on naturally endowed phytonutrients being an alternative in managing the menace. Therefore, the current study was designed to investigate some antioxidant parameters embedded in the extract of Dialium indium (DI) fruit pulp and also, to elucidate its antidiabetic potentials through the inhibition of two key carbohydrate-metabolizing enzymes such as α-amylase and intestinal α-glucosidase. Hydro-ethanolic extract of DI fruit pulp was used for the antioxidants and enzyme inhibitory bioassays through various convectional antioxidant assay methods in vitro. In the results, total phenolic content of the extract had; 6.74 ±3.38 mg GAE.g-1, total flavonoid contents; 0.02 ±0.01 mg QE.g-1 and FRAP; 0.84  ±0.47 mg AAE.g-1 dried sample. Also, there was a marked significant (p <0.05) difference observed in the inhibition of α-amylase and intestinal α-glucosidase by the different concentrations of the extract used in concentration-dependent manner with their different EC50. The inhibition demonstrated against these two carbohydrate metabolizing enzymes possibly could be through the embedded antioxidant potentials of the fruit pulp and this if properly harnessed, it could be helpful in the management of type 2 diabetes.

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References

Ademiluyi, A. Oboh, G. 2013. Soybean phenolic-rich extracts inhibit key-enzymes linked to type 2 diabetes (α-amylase and α -glucosidase) and hypertension (angiotensin I converting enzyme) in vitro. Experimental and Toxicologic Pathology, vol. 65, p. 305-309. https://doi.org/10.1016/j.etp.2011.09.005 PMid:22005499

Afolabi O. B, Oloyede O. I, Obajuluwa A. I, Adewale O. B, Olayide I. I, Balogun B. D, Fadugba A. 2018a. Antioxidant properties and inhibitory potentials of different solvent-fractions of Dialium indium pulp (African Black velvet tamarind) against Fe2+- induced oxidative stress in rat tissues - In vitro. Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, p. 70-78. https://doi.org/10.5219/825

Afolabi, O. B., Oloyede, O. I. 2014. Antioxidant properties of the extracts of Talinum triangulare and its Effect on Antioxidant enzymes in tissue homogenate of swiss albino rat. Toxicology International, vol. 21, no. 3, p. 307-313. https://doi.org/10.4103/0971-6580.155377 PMid:25948971

Afolabi, O. B., Oloyede, O. I., Olayide, I. I., Obafemi, T. O., Awe, J. O., Afolabi, B. A., Onikani, S. A. 2015. Antioxidant enhancing ability of different solvents extractable components of Talinum traingulare in some selected tissue homogenates of albino rat-in vitro. Journal of Applied Pharmaceutical Science, vol. 5, no. 9, p. 56-61. https://doi.org/10.7324/JAPS.2015.50911

Afolabi, O. B., Oloyede, O. I., Agunbiade, S. O. 2018b. Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe2+-induced pancreatic oxidative stress. Journal of Integrative Medicine, Epub ahead of print.

Bao, J., Cai, Y., Sun, M., Wang, G., Corke, H. 2005. Anthocyanins, flavonols and free radical scavenging activity of Chinese bayberry (Myrica rubra) extracts and their color properties and stability. Journal of Agricultural and Food Chemistry, vol. 53, no. 6, p. 2327-2332. https://doi.org/10.1021/jf048312z PMid:15769176

Beverley, B., Eschwège, E. 2003. The diagnosis and classification of diabetes and impaired glucose tolerance. In John, C. P. et al. Textbook of Diabetes. New Jersey : Wiley-Blackwell, p. 2.1-2.11. ISBN 978-0632059157.

Bravo, L. 1998. Polyphenols: Chemistry, dietary sources, metabolism, and nutritional significance. Nutrition Reviews, vol. 56, no. 11, p. 317-333. https://doi.org/10.1111/j.1753-4887.1998.tb01670.x PMid:9838798

Dave, G. S., Kalia, K. 2007. Hyperglycemia induced oxidative stress in type-1 and type-2 diabetic patients with and without nephropathy. Cellular and Molecular Biology, vol. 53, no. 5, p. 68-78. PMid:17543235

Dawra, R. K., Makkar, H. P. 1988. Singh B. Protein-binding capacity of micro quantities of tannins. Analytical Biochemistry, vol. 170, no. 1, p. 50-53. https://doi.org/10.1016/0003-2697(88)90088-7

Erasto, P., Adebola, P. O., Grierson, D. S., Afolayan, A. J. 2005. An ethnobotanical study of the plants used for the treatment of diabetes in the eastern Cape province, South Africa. African Journal of Biotechnology, vol. 4, p. 1458-1460.

Gruenwald, J., Freder, J., Armbruester, N. 2010. Cinnamon and health. Critical Reviews in Food Science and Nutrition, vol. 50, no. 9, p. 822-834. https://doi.org/10.1080/10408390902773052 PMid:20924865

Gyamfi, M. A., Yonamine, M., Aniya, Y. 1999. Free radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally induced liver injuries. General Pharmacology, vol. 32, no. 6, p. 661-667. https://doi.org/10.1016/S0306-3623(98)00238-9

Halliwell, B., Gutteridge, J. M. 1981. Formation of thiobarbituric-acid-reactive substance from deoxyribose in the presence of iron salts: the role of superoxide and hydroxyl radicals. FEBS Letters, vol. 128, no. 2, p. 347-352. https://doi.org/10.1016/0014-5793(81)80114-7

Hilary, K., Ronald, E. A., William, H. H. 1998. Global burden of diabetes 1995-2005. Prevalence, numerical estimates and projections. Diabetes care, vol. 21, no. 9, p. 141-143.

Hung, H. Y., Qian, K., Morris-Natschke, S. L., Hsu, C. S., Lee, K. H. 2012. Recent discovery of plant-derived anti-diabetic natural products. Natural Product Reports, vol. 29, no. 5, p. 580-606. https://doi.org/10.1039/c2np00074a PMid:22491825

Inga, P., Mark, I. M., Cecilia, M. L. 2008. Type 2 diabetes: new genes, new understanding. Trends in Genetics, vol. 24, no. 12, p. 613-621. https://doi.org/10.1016/j.tig.2008.09.004 PMid:18952314

Inglett, G. E., Chen, D. 2011. Contents of phenolics and flavonoids and antioxidant activities in skin, pulp, and seeds of miracle fruit. Journal of Food Science, vol. 76, no. 3, p. 479-482. https://doi.org/10.1111/j.1750-3841.2011.02106.x PMid:21535817

Jalapure, S. S., Patil, M. B., Pai, A., Salahuddin, M. D. 2004. Anti-diabetics activity of Cassia auriculata seed in alloxan induced diabetes rats. Nigerian Journal of Natural Products and Medicine, vol. 8, p. 22-23.

Jo, S. H., Ka, E. H., Lee, H. S., Apostolidis, E., Jang, H. D., Kwon, Y. I. 2009. Comparison of antioxidant potential and rat intestinal α-glucosidases inhibitory activities of quercetin, rutin, and isoquercetin. International Journal of Applied Research in Natural Products, vol. 2, p. 52-60.

Krentz, A. J., Bailey, C. J. 2005. Oral antidiabetic agents: Current role in type 2 diabetes mellitus. Drug, vol. 65, no. 3, p. 385-411. https://doi.org/10.2165/00003495-200565030-00005

Kwon, Y. I., Apostolidis, E., Kim, Y. C., Shetty, K. 2007. Health benefits of traditional corn, beans and pumpkin: In vitro studies for hyperglycemia and hypertension management. Journal of Medicinal Food, vol. 10, no. 2, p. 266-275. https://doi.org/10.1089/jmf.2006.234 PMid:17651062

Leszek, C., László, B., Svetlana, B., Agata, B., Jan, B., Katarzyna C, Marek H., Andrej J., Mladen K., Nebojsa L., Emil M., Dario R., Gabriela R., Tsvetalina T., Tamás V., Bogumił W. and Nadia Z. 2014. Self-Monitoring of Blood Glucose in Diabetes: From Evidence to Clinical Reality in Central and Eastern Europe - Recommendations from the International Central-Eastern European Expert Group. Diabetes Technology and Therapeutics, vol. 16, no. 7, p. 460-475. https://doi.org/10.1089/dia.2013.0302 PMid:24716890

Madhusudhan, T., Kirankumar, H. 2015. In-vitro α-amylase and α-glucosidase inhibitory activity of Adiantum caudatum Linn. and Celosia argentea Linn. extracts and fractions. Indian Journal of Pharmacology, vol. 47, no. 4, p. 425-429. https://doi.org/10.4103/0253-7613.161270 PMid:26288477

Materska, M., Perucka, I. 2005. Antioxidant activity of the main phenolic compounds isolated from hot pepper fruits (Capsicum annuum L.). Journal of Agricultural and Food Chemistry, vol. 53, no. 5, p. 1730-1758. https://doi.org/10.1021/jf035331k PMid:15740069

McCue, P., Kwon, Y. I., Shetty, K. 2005. Anti-diabetic and antihypertensive potential of sprouted and solid-state bioprocessed soybean. Asia Pacific Journal of Clinical Nutrition, vol. 14, no. 2, p. 145-152. PMid:15927931

Mercocci, L., Marguire, J. J., Droy-Lefaiz, M. T., Parker, L. 1994. The nitric oxide-scavenging properties of Ginko biloba extract EGB 761. Biochemical and Biophysical Research Communications, vol. 201, no. 2, p. 748-755. https://doi.org/10.1006/bbrc.1994.1764

Nishikawa, T., Edelstein, D., Du, X. L. 2000. Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature, vol. 404, no. 6779, p. 787-790. https://doi.org/10.1038/35008121 PMid:10783895

Nwaukwu, I. A, Ikechi, N. C. 2012. Biochemical Changes Induced by the Effect of Six Pathogenic Fungi on Dialium guineense: Black Velvet Edible Fruit. IOSR International Journal of Pharmacy and Biological Sciences, vol. 2, no. 4, p. 20-24. https://doi.org/10.9790/3008-0242024

Obafemi, T. O., Onasanya, A., Olaoye, O. A., Afolabi, O.B., Awe, O. J, Anadozie, S. O., Ojo, O. A., Adedeji, E. 2016. In vitro Antioxidant Activity and High Performance Liquid Chromatography (HPLC) Analysis of methanolic extract of Platycerium angolense. International Journal of Pharmacognosy and Phytochemical Research, vol. 8, no.9, p. 1497-1502.

Oberley, L. W. 1988. Free radicals and diabetes. Free Radical Biology and Medicine, vol. 5, no. 2, p. 113-124. https://doi.org/10.1016/0891-5849(88)90036-6

Oyedemi, S. O., Oyedemi, B. O., Ijeh, I. I., Ohanyerem, P. M. E., Coopoosamy, R. M., Aiyegoro, O. A. 2017. Alpha-amylase inhibition and antioxidative Capacity of some antidiabetic plants used by the traditional healers in southeastern Nigeria. The Scientific World Journal, vol. 2017, p. 1-11. https://doi.org/10.1155/2017/3592491 PMid:28367491

Porte, D., Kahn, S. E. 2001. Beta-cell dysfunction and failure in type 2 diabetes: Potential mechanisms. Diabetes, vol. 1, p. 160-116. https://doi.org/10.2337/diabetes.50.2007.S160

Pulido, R., Bravo, L., Saura-Calixto, F. 2000. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of Agricultural and Food Chemistry, vol. 48, no. 8, p. 3396-3402. https://doi.org/10.1021/jf9913458 PMid:10956123

Rhabasa-Lhoret, R., Chiasson, J. L. 2004. Alpha-glucosidase inhibition. In Defronzo, R. A. et al. International textbook of diabetes mellitus. 3rd ed. London, UK : John Wiley, 2070 p. ISBN 9780471486558.

Robertson, R. P., Harmon, J. S. 2007. Pancreatic islet β-cell and oxidative stress: the importance of glutathione peroxidase. FEBS Letters, vol. 581, no. 19, p. 3743-3748. https://doi.org/10.1016/j.febslet.2007.03.087 PMid:17433304

Shai, L. J., Masoko, P., Mokgotho, M. P., Magano, S. R., Mogale, A. M., Boaduo, N., Eloff, J. N. 2010. Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in phalaborwa, South Africa. South African Journal of Botany, vol. 76, no. 3, p. 465-470. https://doi.org/10.1016/j.sajb.2010.03.002

Shim, Y. J., Doo, H. K., Ahn, S. Y., Kim, Y. S., Seong, J. K., Park, I. S., Min, B. H. 2003. Inhibitory effect of aqueous extract from the gal of Rhuz chinesis on alpha-glucosidase activities and postprandial blood glucose. Journal of Ethnopharmacology, vol. 85, no. 2-3, p. 283-287. https://doi.org/10.1016/S0378-8741(02)00370-7

Singh, S. K., Rai, P. K., Jaiswal, D., Watal, G. 2007. Evidence-based critical evaluation of glycemic potential of Cynodon dactylon. Evidence-Based Complementary and Alternative Medicine, vol. 5, no. 4, p. 415-420. https://doi.org/10.1093/ecam/nem044 PMid:18955211

Singleton, V. L., Orthofer, R., Lamuela-Raventos, R. M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. In Packer, L. Methods in enzymology. Oxidants and antioxidants Part A. San Diego, California : Academic Press, p.152-178. ISBN 978-12-182200-2.

Stein, S. A., Lamos, E. M., Davis, S. N. 2013. A review of the efficacy and safety of oral antidiabetic drugs. Expert Opinion on Drug Safety, vol. 12, no. 2, p. 153-175. https://doi.org/10.1517/14740338.2013.752813 PMid:23241069

Zar, J. H. 1984. Biostatistical analysis. 2nd ed. USA : Prentice-Hall Inc, 718p. ISBN 9780130779250.

Zimmet, P., Alberti, K. G., Shaw, J. 2001. Global and societal implications of the diabetesepidemic. Nature, vol. 414, no. 6865, p. 782-787. https://doi.org/10.1038/414782a PMid:11742409

Ziping, X., Weihua, F., Jiankang, C., Dongdong, C., Weibo, J. 2009. Antioxidant activity and total phenolic contents in peel and pulp of Chinese Jujube (Ziziphus jujuba Mill) fruits. Journal of Food Biochemistry, vol. 33, no. 5, p. 613-629. https://doi.org/10.1111/j.1745-4514.2009.00241.x

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Published

2018-04-13

How to Cite

Afolabi, O. B., Oloyede, O. I., Ojo, A. A., Onansanya, A. A., Agunbiade, S. O., Ajiboye, B. O., Johnson, J. ., & Peters, O. A. (2018). In vitro antioxidant potential and inhibitory effect of hydro-ethanolic extract from African black velvet tamarind (Dialium indium) pulp on type 2 diabetes linked enzymes. Potravinarstvo Slovak Journal of Food Sciences, 12(1), 413–421. https://doi.org/10.5219/911

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Vol. 12 No. 1 (2018): Potravinarstvo Slovak Journal of Food Sciences

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