Effect of rana galamensis-based diet on the activities of some enzymes and histopathology of selected tissues of albino rats
DOI:
https://doi.org/10.5219/642Keywords:
Rana galamensis, ALP, AST, ALT, γGT, histological examinationAbstract
The effect of Rana galamensis-based diet on the activities of some enzymes and histopathology of selected tissues of albino rats was investigated for eight weeks. A total of sixteen albino rats weighing between 29.15 and 26.01g (21 days old) were divided into two groups. The first group contains animals fed on casein-based diet (control); the second group was fed on Rana galamensis-based diet. The animals were fed with their appropriate diet on daily basis and on the eight weeks of the experiment the animals were sacrificed using diethyl ether as anesthesia, blood was collected by cardiac puncture and organs of interest were harvested. Thereafter, organ to body weight ratio, some biochemical parameters and histopathology examination were carried out. There was no significant difference (p >0.05) in the organ to body weight ratio of the animals fed on control and Rana galamensis-based diets. Also, there was no significant different (p >0.05) in the activities of all the enzymes (ALP [alkaline phosphatase], AST [asparate transaminase], ALT [alanine transaminase], and γGT [gamma glutamyl transferase]) investigated in the selected tissues and serum of rats fed on Rana galamensis- based diet when compared with the control. In addition, histological examinations of hepatocyte's rats fed on Rana galamensis- based diet show normal architecture structure when compared with the control. The insignificant different in the activities of all the enzymes studies (ALP, AST, ALT and γGT) indicated no organ damage, supported by the normal histology studies. The obtained results may imply that Rana galamensis is safe for consumption.
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Abubakar, M. G., Lawal, A., Suleiman, B., Abdullahi, K. 2010. Hepatorenal toxicity studies of sub-chronic administration of calyx aqueous extracts of Hibiscus sabdariffa in albino rats. Bayero Journal of Pure and Applied Sciences, vol. 3, no. 1, p. 16-19. https://doi.org/10.4314/bajopas.v3i1.58550
Adamu, S. O., Johnson, T. L. 1997. Statistics for beginners. Book 1. Ibadan, Nigeria : SAAL Publication. p. 184-199. ISBN:978-1601890917
Ajiboye, B. O., Muhammad, N. O., Abdulsalam, M. S. Oloyede, O. B. 2014. Nutritional evaluation of Rana galamensis -based diet in albino rats. International Journal of Applied Research and Technology., vol. 3, no. 6, p. 35-40.
Ajiboye, B. O., Muhammad, N. O. 2015. Investigation into the haematological parameters of albino rats fed with Rana galamensis-based diet as an alternative source of protein in animal feed. Journal of Global Agriculture and Ecology, vol. 2, no. 4, p. 112-116.
Akanji, M. A. 1986. A comparative biochemical study of the interaction of some trypanocides with rat tissue cellular system : dissertation thesis. Ile-Ife : University of Ife, p. 34-48.
Akanji, M. A., Ngaha, E. O. 1989. Effect of repeated administration of berenil in urinary enzyme excretion with corresponding tissue pattern in rat. Pharmacology and Toxicology, vol. 64, no. 3, p. 272-275. https://doi.org/10.1111/j.1600-0773.1989.tb00645.x PMid:2726690
Akanji, M. A., Olagoke, O. A., Oloyede, O. B. 1993. Effect of chronic consumption of metabisulphite on the integrity of rat cellular system. Toxicology, vol. 8, no. 3, p. 173-179. https://doi.org/10.1016/0300-483X(93)90010-P
Akanji, M. A., Yakubu, M. T., Kazeem, M. I. 2013. Hypolipidemic and toxicological potential of aqueous extract of Rauvolfia vomitoria afzel root in wistrar rats. Journal of medical sciences, vol. 13, p. 253-260. https://doi.org/10.3923/jms.2013.253.260
Bonting, S. L., Pollark, V. E., Muchrcke, R. L., Kack, R. M. C. 1960. Quantitative histochemistry of the nephron II: Alkaline phosphatase activity in man and other species. Journal of Clinical Investigation, vol. 39, no. 9, p. 1372-1380. https://doi.org/10.1172/JCI104156 PMid:13802623
Drury, R. A. B., Wallington, E. A. 1973. Tissue Histology. In: Carleton's Histological Technique. 4th ed. New York : Oxford University Press, p. 58. ISBN 13: 9780192613103
Bell, G. H., Donald, E. S., Collin, R. P. 1976. Textbook of Physiology and Biochemistry. 8th ed., Harcourt Brace : Churchill Livingstone. ISBN-13: 9780443014369.
Harvey, R. A., Ferrier, D. H. 2010. Lippincott's Illustrated Reviews. Lippincot William and Wilkins. 5th Edition. ISBN-13: 978-1451175622.
Krause, W. J. 2001. The art of examining and interpreting histologic preparations. A student handbook. UK : Partheton Publishing Group. p. 9-10. ISBN 9781850702771.
Mayne, P. D.1998. Clinical Chemistry in Diagnosis and Treatment. 6th Edition. UK, London : Arnold International Students. p. 196-205. ISBN-13: 978-0340576472.
Moore, K. L., Dalley, A. F. 1999. Clinically Oriented Anatomy. 4th ed. Philadelphia : Lippincoth Williams and Wilkins, a Woiler Klummer Company. p 263-271. ISBN: 9780683061413
Muhammad, N. O., Ajiboye, B. O. 2010. Nutrient composition of Rana galamensis. African Journal of Food Science and Technology, vol. 1, no. 1, p. 27-30.
Murray, R. K., Bender, D. A., Botham, K. A., Kennelly, P. J., Rodwell, V. W., Weil, P. A. 2009. Harper's Illustrated Biochemistry. McGraw Hill Lange. 28th Edition
Naik, P. 2011. Biochemistry Textbook. Jaypee Brother Medical Publisher. 3 rd Edition.
Oloyede, O. B., Fowomola, M. A. 2003. Effects of fermentation on some nutrient contents and protein quality of soy bean seeds. Nigerian Journal of Pure and Applied Science, vol. 18, p. 1380-1386.
Persijin, J. 1976. Determination of serum gamma glutamyl transferase. Clinical Chemistry and Biochemistry, vol. 9, no. 14, p. 421-427.
Reitman, S., Frankel, S. O. 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Ameriacn Journal of Clinical Pathology, vol. 28, no. 1, p. 56-57. https://doi.org/10.1093/ajcp/28.1.56 PMid:13458125
Shahjahan, M., Sabitha, K. E., Mallika, J., Shayamala- Devi, C. S. 2004. Effect of Solannum trilobtum against carbon tetrachloride induced hepatic damage in albino rats. Indian Journal of Medical Research, vol. 120, no. 3, p. 194-198. PMid:15489557
Wright, P. J., Leathwood, P. D., Plummer, D. T. 1972. Enzymes in rat urine: alkaline phosphatase. Enzymologia, vol. 42, no. 4, p. 317-327. PMid:4337755
Yakubu, M. T., Akanji, M., Salau, I. O. 2001. Protective effect of ascorbic acid on some selected tissues of ranitidine-treated rats. Nigerian Society of Biochemistry and Molecular Biology, vol. 16, no. 2, p. 177-182.
Yakubu, M. T., Akanji, M. A., Oladiji, A. T. 2008. Effect of oral administration of aqueous extract of Fadogia agrestis stem on some testicular function indices of male rats. Journal of Ethnopharmacology, vol. 111, no. 2, p. 288-292. https://doi.org/10.1016/j.jep.2007.10.004 PMid:18023305
Yakubu, M. T., Bilbis, L. S., Lawal, M., Akanji, M. A. 2003. Evaluation of selected parameters of rat liver and kidney function repeated administertaion of yohimbine. Biokemistri, vol. 15, no. 2, p. 50-56.
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