Determination of acrylamide in food by gas and liquid chromatography-mass spectrometry

Miriam  Vlčáková; Michaela  Vieriková

doi:10.5219/61

Authors

Miriam Vlčáková State veterinary and food institution, Janoškova 1611/58, 026 01 Dolný Kubín, Slovakia
Michaela Vieriková State veterinary and food institution, Janoškova 1611/58, 026 01 Dolný Kubín, Slovakia

DOI:

https://doi.org/10.5219/61

Keywords:

acrylamide, food, LC-MS,

Abstract

Acrylamide in food was determined by gas chromatography - mass spectrometry (GC-MS) after bromination of acrylamide and underivatized acrylamide was quantified by ultra performance liquid chromatography -mass spectrometry (UPLC-MS). Two different sample preparation methods were used and optimised. The GC-MS method was used for various food matrices like breads, potato crisps, potato crackers, french fries. The UPLC-MS method was used for analysis of coffee. The limit of detection and limit of quantification for acrylamide were 7 µg.kg^-1and 20 µg.kg^-1 by GC-MS, 9 µg.kg^-1and 30 µg.kg^-1by UPLC-MS. For both methods the reproducibility, given as relative standard deviation was < 5% , and the recovery was close to 100 %.

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