Changes in lipid content of wine yeasts during fermentation by immobilized cells
Keywords:Saccharomyces cerevisiae, etanol, imobilizÃ¡cia, lipidy
Comparison of the lipid composition of immobilised and non-immobilised cells of the wine cell strain Saccharomyces cerevisiae 6C subjected to ethanol stress indicates that the whole impact of the ethanol stress on the fatty acids composition is less influenced with immobilised cells as with non- immobilised ones. The ethanol stress raised in immobilised and free cells occurrence of palmitoleic acid to the detriment of palmitic acid. The character of changes in lipid composition during immobilisation probably has an impact upon slightly increased stress resistance. The immobilised cells are as well resistive against passive membrane fluidisation by ethanol.
CARMAN, G. M., HENRY, S. A. 1989. Phospholipid Biosynthesis in Yeast. In An.Rev.Biochem., vol. 58, 1989, p. 635-667. DOI: https://doi.org/10.1146/annurev.bi.58.070189.003223
FOLCH, J., LEES, M., SLOANE-STANLEY, G. H. 1957. A simple method for the isolation and purification of total lipides from animal tissue. In J.Biol.Chem., vol. 225, 1957, p. 497-509. DOI: https://doi.org/10.1016/S0021-9258(18)64849-5
JIRKŮ, V. 1999. Whole cell immobilization as a means of enhancing ethanol tolerance. In J. Ind. Microbiol. Biotechnol., vol. 22, 1999, p. 147-151.
MISHRA, P., PRASAD, R. 1989. Relationship between ethanol tolerance and fatty acyl composition of Saccharomyces cerevisiae. In Appl. Microbiol. Biotechnol., vol. 30, 1989, p. 294-298.
ROTMANN, B. H., REHM, H. J. 1991. Relationship between fermentation capability and fatty acid composition of free and immobilized Saccharomyces cerevisiae. In Appl. Microbiol. Biotechnol., vol. 34, 1991, p. 502-508. DOI: https://doi.org/10.1007/BF00180578
SHOBAYASHI, M., SHIN-ICHIRO MITSUEDA, S., AGO, M., FUJII, T., IWASHITA, K., IEFUJI, H. 2005. Effects of Culture Conditions on Ergosterol Biosynthesis by Saccharomyces cerevisiae. In Biosci. Biotechnol. Biochem., vol. 69, 2005, p. 2381-2388.
ŠAJBIDOR, J. 1997. Effect of some environmental factors on the content and composition of microbial membrane lipids. In Crit. Rev. Biotechnol., vol. 17, 1997, p. 87-103.
YOU, K.M., ROSENFIELD, C. L., KNIPPLE, D.C. 2003. Ethanol Tolerance in the Yeast Saccharomyces cerevisiae Is Dependent on Cellular Oleic Acid Content. In Appl. Env. Microbiol., vol. 69, 2003, p. 1499-1503.
How to Cite
This license permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.