Changes in lipid content of wine yeasts during fermentation by immobilized cells
DOI:
https://doi.org/10.5219/56Keywords:
Saccharomyces cerevisiae, etanol, imobilizácia, lipidyAbstract
Comparison of the lipid composition of immobilised and non-immobilised cells of the wine cell strain Saccharomyces cerevisiae 6C subjected to ethanol stress indicates that the whole impact of the ethanol stress on the fatty acids composition is less influenced with immobilised cells as with non- immobilised ones. The ethanol stress raised in immobilised and free cells occurrence of palmitoleic acid to the detriment of palmitic acid. The character of changes in lipid composition during immobilisation probably has an impact upon slightly increased stress resistance. The immobilised cells are as well resistive against passive membrane fluidisation by ethanol.
Downloads
References
CARMAN, G. M., HENRY, S. A. 1989. Phospholipid Biosynthesis in Yeast. In An.Rev.Biochem., vol. 58, 1989, p. 635-667.
FOLCH, J., LEES, M., SLOANE-STANLEY, G. H. 1957. A simple method for the isolation and purification of total lipides from animal tissue. In J.Biol.Chem., vol. 225, 1957, p. 497-509.
JIRKŮ, V. 1999. Whole cell immobilization as a means of enhancing ethanol tolerance. In J. Ind. Microbiol. Biotechnol., vol. 22, 1999, p. 147-151.
https://doi.org/10.1038/sj.jim.2900620
MISHRA, P., PRASAD, R. 1989. Relationship between ethanol tolerance and fatty acyl composition of Saccharomyces cerevisiae. In Appl. Microbiol. Biotechnol., vol. 30, 1989, p. 294-298.
https://doi.org/10.1007/BF00256221
ROTMANN, B. H., REHM, H. J. 1991. Relationship between fermentation capability and fatty acid composition of free and immobilized Saccharomyces cerevisiae. In Appl. Microbiol. Biotechnol., vol. 34, 1991, p. 502-508.
SHOBAYASHI, M., SHIN-ICHIRO MITSUEDA, S., AGO, M., FUJII, T., IWASHITA, K., IEFUJI, H. 2005. Effects of Culture Conditions on Ergosterol Biosynthesis by Saccharomyces cerevisiae. In Biosci. Biotechnol. Biochem., vol. 69, 2005, p. 2381-2388.
https://doi.org/10.1271/bbb.69.2381
ŠAJBIDOR, J. 1997. Effect of some environmental factors on the content and composition of microbial membrane lipids. In Crit. Rev. Biotechnol., vol. 17, 1997, p. 87-103.
https://doi.org/10.3109/07388559709146608
PMid:9192472
YOU, K.M., ROSENFIELD, C. L., KNIPPLE, D.C. 2003. Ethanol Tolerance in the Yeast Saccharomyces cerevisiae Is Dependent on Cellular Oleic Acid Content. In Appl. Env. Microbiol., vol. 69, 2003, p. 1499-1503.
https://doi.org/10.1128/AEM.69.3.1499-1503.2003
PMid:12620835
PMCid:150070
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
