Composition and microstructure alteration of triticale grain surface after processing by enzymes of cellulase complex
DOI:
https://doi.org/10.5219/411Keywords:
triticale, grain, xylanase, microstructure, antioxidant activityAbstract
It is found that the pericarp tissue of grain have considerable strength and stiffness, that has an adverse effect on quality of whole-grain bread. Thereby, there exists the need for preliminary chemical and biochemical processing of durable cell walls before industrial use. Increasingly used in the production of bread finds an artificial hybrid of the traditional grain crops of wheat and rye - triticale, grain which has high nutritional value. The purpose of this research was to evaluate the influence of cellulose complex (Penicillium canescens) enzymes on composition and microstructure alteration of triticale grain surface, for grain used in baking. Triticale grain was processed by cellulolytic enzyme preparations with different composition (producer is Penicillium canescens). During experiment it is found that triticale grain processing by enzymes of cellulase complex leads to an increase in the content of water-soluble pentosans by 36.3 - 39.2%. The total amount of low molecular sugars increased by 3.8 - 10.5 %. Studies show that under the influence of enzymes the microstructure of the triticale grain surface is changing. Microphotographs characterizing grain surface structure alteration in dynamic (every 2 hours) during 10 hours of substrate hydrolysis are shown. It is found that the depth and direction of destruction process for non-starch polysaccharides of grain integument are determined by the composition of the enzyme complex preparation and duration of exposure. It is found, that xylanase involved in the modification of hemicelluloses fiber having both longitudinal and radial orientation. Hydrolysis of non-starch polysaccharides from grain shells led to increase of antioxidant activity. Ferulic acid was identified in alcoholic extract of triticale grain after enzymatic hydrolysis under the influence of complex preparation containing cellulase, xylanase and β-glucanase. Grain processing by independent enzymes containing in complex preparation (xylanase and β-glucanase) shows that more significant role in polysaccharide complex composition and grain surface microstructure alteration belongs to xylanase. Grain processing by independent of cellulolytic enzymes may decrease the strength of pericarp tissue of grain and improved sensory characteristics of the bread.
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Andlaver, W., Furst P. 2002. Nutraceuticals a piece of history, present status and outlook. Food Research International. vol. 35, no. 2-3, p. 171-176. https://doi.org/10.1016/S0963-9969(01)00179-X DOI: https://doi.org/10.1016/S0963-9969(01)00179-X
Antoine, C., Peyron, S., Mobille, F., Lapierre, C., Bouchet, B., Abecassis, J., Rouau, X. 2003. Individual contribution of grain outer layers and their cell wall structure to the mechanical properties of wheat bran. Journal of Agricultural and Food Chemistry. vol. 51, no. 7, p. 2026-2033. https://doi.org/10.1021/jf0261598 DOI: https://doi.org/10.1021/jf0261598
Barron, C., Surget, A., Rouau, X. 2007. Relative amoutns of tissues in mature what (Triticum aestivum L.) grain and their carbohydrate and phenolic acid composition. Journal of Cereal Science. vol. 45, no. 1,
p. 88-96. https://doi.org/10.1016/j.jcs.2006.07.004 DOI: https://doi.org/10.1016/j.jcs.2006.07.004
Broun, L., Rosner, B., Willett, W. W., Sacks, F. M. 1999. Cholesterol-lowering effects of dietary fibre: a meta-analysis. The American Journal of Clinical Nutrition. vol. 69, p. 30-42. Available at: http://ajcn.nutrition.org/content/69/1/30.full DOI: https://doi.org/10.1093/ajcn/69.1.30
Cauvain, S. P., Young, L. S. 2007. Technology of Breadmaking: Springer US. 397 p. ISBN 978-3-319-14687-4; https://doi.org/10.1007/978-3-319-14687-4 DOI: https://doi.org/10.1007/978-3-319-14687-4
Charalampopoulos, D., Wang, R., Pandella, S. S., Webb, C. 2002. Application of cereals and cereal components in functional food: a review. International Journal of Food microbiology, vol. 79, no. 1-2, p. 131-141. https://doi.org/10.1016/S0168-1605(02)00187-3 DOI: https://doi.org/10.1016/S0168-1605(02)00187-3
Courtin, C. M., Delcour, J. A. 2002. Arabinoxylans and endoxylanases in wheat flour bread-making. Journal of Cereal Science, vol. 35, no. 3, p. 225-243. https://doi.org/10.1006/jcrs.2001.0433 DOI: https://doi.org/10.1006/jcrs.2001.0433
Courtin, C. M., Roelants, A., Delcour, J. A. 1999. Fractionation–reconstitution experiments provide insight into the role of endoxylanases in bread-making. Journal of Agricultural and Food Chemistry,
vol. 47, no. 5, p. 1870-1877. https://doi.org/10.1021/jf981178w PMid:10552464 DOI: https://doi.org/10.1021/jf981178w
Cummings, J. H., Bingham, S. A., Heaton, K. W., Eastwood M. 1992. Fecal weight, colon cancer risk and the dietary intake of nonstarch polysaccharides (dietary fibre). Gastroenterology. vol. 103, no. 6, p. 1783-1789. PMid:1333426 DOI: https://doi.org/10.1016/0016-5085(92)91435-7
Cummings, J. H., Mann, J. I., Nishida, C., Vorster, H. H. 2009. Dietary fibre: an agreed definition. Lancet. vol. 373, no. 9661, p. 366-66. https://doi.org/10.1016/S0140-6736(09)60117-3 DOI: https://doi.org/10.1016/S0140-6736(09)60117-3
De Vries, R. P., Kester, H. C. M., Poulsen, CH. H., Benen, J. A. E., Visser, J. 2000. Synergy between enzymes from Aspergillus involved in the degradation of plant cell wall polysaccharides. Carbohydrate Research. vol. 327, no. 4, p. 401-410. https://doi.org/10.1016/S0008-6215(00)00066-5 DOI: https://doi.org/10.1016/S0008-6215(00)00066-5
Gebruers, K., Dornez, E., Boros, D., Fras, A., Dynkowska, W., Bedo, Z., Rakszegi, M., Delcour, J. A., Courtin Ch. M. 2008. Variation in the content of dietary fibre and components ther of in wheats in the HEALTHGRAIN diversity screen. Journal of Agricultural and Food Chemistry. vol. 56, no. 21, p. 9740-9749. https://doi.org/10.1021/jf800975w PMid:18921978 DOI: https://doi.org/10.1021/jf800975w
Grigelmo-Miguel, N., Martin-Belloso, O. 1999. Comparison of dietary fibre from by-products of processing fruits and greens and from cereals. Lebensm-Wiss Technology. vol. 32, no. 8, p. 503-508. https://doi.org/10.1006/fstl.1999.0587 DOI: https://doi.org/10.1006/fstl.1999.0587
Jacobs, J., Marquart, L., Slavin, J., Kushi, L. 1998. Whole-grain intake and cancer: an explanded review and meta-analysis. Nutrition and Cancer. vol. 30, no. 2, p. 85-96. https://doi.org/10.1080/01635589809514647 PMid:9589426 DOI: https://doi.org/10.1080/01635589809514647
Jiang, Z., Li, X., Yang, S., Li, L., Tan, S. 2005. Improvement of the breadmaking quality of the flour by the hiperthermophilic xylanase B from Thermotoga Maritime. Food Research International. vol. 38, no. 1, p. 37-43. https://doi.org/10.1016/j.foodres.2004.07.007 DOI: https://doi.org/10.1016/j.foodres.2004.07.007
Hashimoto, S., Shogren, N. D., Pomeranz, Y. 1987. Cereal pentosans: estimation and significance. I. Pentosans in wheat and milled wheat products. Cereal Chemistry. vol. 64, no. 1, p. 30-34. Available at: http://www.aaccnet.org/publications/cc/backissues/1987/documents/64_30.pdf
Havrlentova, M., Petrulakova, Z., Burgarova, A., Gago, F., Hlinkova, A., Sturdik, E. 2011. Cereal β-glucans and their significance for the preparation of functional food: a review. Czech Journal of Food Sciences. vol. 29, no 1, p. 1-14. Available at: http://www.agriculturejournals.cz/publicFiles/34997.pdf
Izydorczyk, M. S., Biliarderis, C. G. 1995. Cereal arabinoxylans: advances in structure and physico-chemical properties. Carbohydrate Polymers. vol. 28, no. 1, p. 33-48. https://doi.org/10.1016/0144-8617(95)00077-1 DOI: https://doi.org/10.1016/0144-8617(95)00077-1
Lazaridou, A., Biliaderis, C. G. 2007. Molecular aspects of cereal β-glucan functionality: Physical properties, technological applications and physiological effects. Journal of Cereal Science. vol. 46, no. 2, p. 101-118. https://doi.org/10.1016/j.jcs.2007.05.003 DOI: https://doi.org/10.1016/j.jcs.2007.05.003
Leggio, L. L., Pickersgill, R. W. 1999. Xylanase-oligosaccharide interactions studied by a competitive enzyme assay. Enzyme and microbial technology. vol. 25, no. 8-9, p. 701-709. https://doi.org/10.1016/S0141-0229(99)00103-9 DOI: https://doi.org/10.1016/S0141-0229(99)00103-9
Lu, Z. X., Walker, K. Z., Muir, J. G., O'Dea, K. 2004. Arabinoxylan fibre improves metaoblic control in peoplewith Type 2 diabetes.European Journal of Clinical Nutrition. vol. 53, p. 621-628. https://doi.org/10.1038/sj.ejcn.1601857 DOI: https://doi.org/10.1038/sj.ejcn.1601857
Mcintosh, G. H., Whyte, J., Mcarthur, R., Nestel, P. 1991. Barley and wheat foods: influence on plasma cholesterol concerntations in hypercholesterolemeic men. The American Journal of Clinical Nutrition. vol. 53, no. 5, p. 1205-1209. PMid:1850576 DOI: https://doi.org/10.1093/ajcn/53.5.1205
Parkkonen, T., Tervila-Wilo, A., Hopeakoski-Nurminen, N., Morgan, A., Poutanen, K., Autio, K. 1997. Changes in Wheat Microstructure Following in Vitro Digestion. Acta Agriculturae Scandinavica - Section B Soil and Plant Science. vol. 47, no. 1, p. 43-47. https://doi.org/10.1080/09064719709362437 DOI: https://doi.org/10.1080/09064719709362437
Peterson, K., Nordlang, E., Tornberg, E., Eliasson, A. CH., Buchert, J. 2013. Impact of the cell wall destroying enzymes on the water-holding capacity and solubility of dietary fiber in the rye and wheat bran. Journal of the science of food and agriculture. vol. 93, no. 4, p. 882-889. https://doi.org/10.1002/jsfa.5816 PMid:22865289 DOI: https://doi.org/10.1002/jsfa.5816
Said, F., Pasha, I., Anjum, F. M., Sultan, M. 2011. Arabinoxylans and arabinogalactans: a Comprehensive treatise (Review). Critical Reviews in food science and nutrition. vol. 51, no. 5, p. 467-476. https://doi.org/10.1080/10408391003681418 PMid:21491271 DOI: https://doi.org/10.1080/10408391003681418
Silva, В. A., Ferreres, F., Malva, J. O., Dias, A. C. P. 2005. Phytochemical and antioxidant characterization of Hypericum perforatum alcoholic extracts. Food Chemistry. vol. 90, no. 1-2, p. 157-167. https://doi.org/10.1016/j.foodchem.2004.03.049 DOI: https://doi.org/10.1016/j.foodchem.2004.03.049
Sinitsyna, O. A., Bukhtoyarov, F. E., Gusakov, A. V., Sinitsyn, A. P., Okunev, O. N., Bekkarevitch, A. O., Vinetsky, Yu. P. 2003. Isolation and properties of major components of Penicillium canescens extracellular enzyme complex. Biochemistry (Moscow). vol. 68, no 11, p. 1200-1209. https://doi.org/10.1023/B:BIRY.0000009134.48246.7e DOI: https://doi.org/10.1023/B:BIRY.0000009134.48246.7e
Tervila-Wilo, A., Parkkonen, T., Morgan, A., Hopeakoski-Nurminen, N., Poutanen, K., Heikkinen, P., Autio, K. 1996. In vitro digestion of wheat microstructure with xylanase and cellulase from Trichoderma reesei. Journal of Cereal Science. vol. 24, no. 3, p. 215-225. https://doi.org/10.1006/jcrs.1996.0054 DOI: https://doi.org/10.1006/jcrs.1996.0054
Ulvsov, P., Øbro, J., Hayash, T., Mikkelsen, J. D. 2011. Enzymatic Modification of Plant Cell Wall Polysaccharides. Annual Plant Reviews: Plant Polysaccharides, Biosynthesis and Bioengineering, Blackwell Publishing Ltd., vol. 41, p. 367-387. https://doi.org/10.1002/9781444391015.ch15 DOI: https://doi.org/10.1002/9781444391015.ch15
Vaikousi, H., Biliaderis, C. G., Izydorczyk, M. S. 2004. Solution flow behavior and gelling properties of water-soluble barley (1→3, 1→4)-β-glucans varying in molecular size. Journal of Cereal Science. vol. 39, no. 1, p. 119-137. https://doi.org/10.1016/j.jcs.2003.09.001 DOI: https://doi.org/10.1016/j.jcs.2003.09.001
Van Craeyveld, V., Holopainen, U., Selinheimo, E., Poutanen, K., Delcour, J. A., Courtin, C. M. 2009. Extensive dry ball milling of wheat and rye bran leads to in situ production of arabinoxylan oligosaccharides through nanoscale fragmentation. Journal of Agricultural and Food Chemistry. vol. 57, no. 18, p. 8467-8473. https://doi.org/10.1021/jf901870r PMid:19754173 DOI: https://doi.org/10.1021/jf901870r
Ermakov, A. I. 1972. Methods for biochemical study of plants. Leningrad: Kolos. 456 p.
Kuznetsova, E. A., Cherepnina, L. V. 2010. Mathematical explanation of the enzyme preparations selection in the technology of triticale grain bread. Storage and processing of agricultural raw materials. M. "Food Industry"(Moscow). no. 2, p. 42-44.
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