Low administration of bee pollen in the diet affects qualitative histological characteristics of bone in male rats

Authors

  • Monika Martiniaková Constantine the Philosopher University, Faculty of Natural Sciences, Department of Zoology and Anthropology, 949 74 Nitra
  • Ivana Boboňová Constantine the Philosopher University, Faculty of Natural Sciences, Department of Zoology and Anthropology, 949 74 Nitra
  • Radoslav Omelka Constantine the Philosopher University, Faculty of Natural Sciences, Department of Botany and Genetics, 949 74 Nitra
  • Hana Ďúranová Constantine the Philosopher University, Faculty of Natural Sciences, Department of Zoology and Anthropology, 949 74 Nitra
  • Ramona Babosová Constantine the Philosopher University, Faculty of Natural Sciences, Department of Zoology and Anthropology, 949 74 Nitra
  • Robert Stawarz Krakow Pedagogical University, Institute of Biology, 31 054 Krakow
  • Róbert Toman Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Veterinary Sciences, 949 76 Nitra

DOI:

https://doi.org/10.5219/396

Keywords:

bee pollen, femoral bone, rat

Abstract

Bee pollen is often used as a dietary additive because it contains proteins and is rich in vitamins, minerals and phytochemicals. However, its impact on growth characteristics and bone microstructure is still poorly understood. Therefore, the objective of this study was to investigate the effect of low administration of bee pollen on selected growth characteristics and histological structure of femoral bones in rats. For this purpose, 1-month-old male Wistar rats were randomly divided into two groups of 5 animals each. In the control group (CG), rats were fed a commercial diet throughout the experiment (90 days). Rats of experimental group (EG) received standard diets with a 0.2% addition of bee pollen for the same time period of treatment. At the end of the experiment, macroscopical and microscopical structures of femoral bones from all rats were analysed using analytical scales, sliding instrument, polarized light microscopy and atomic absorption spectrophotometry. The statistical analysis of obtained data did not reveal significant differences for body weight, femoral weight, femoral length, and cortical bone thickness between both investigated groups of rats. However, a higher number of primary and secondary osteons was observed in the central area of substantia compacta and near periosteal surfaces in rats from the EG group. Histomorphometrical data of primary osteons' vascular canals, Haversian canals and secondary osteons did not differ between rats from both groups. Also, concentrations of Ca, Mg, Fe and Zn in the bones of rats from the EG group were similar to those from CG group. Our results indicate that 0.2% concentration of bee pollen in the diet significantly affects qualitative histological characteristics of femoral bones in rats. On the other hand, it has no impact on the size of primary and secondary osteons and on the content of Ca, Mg, Fe and Zn in the bones of male rats.

Downloads

Download data is not yet available.

References

Almeida-Muradian, L. B., Pamplona, L. C., Coimbra, S., Barth, O. M. 2005. Chemical composition and botanical evaluation of dried bee pollen pellets. Journal of Food Composition and Analysis, vol. 18, no. 1, p. 105-111. https://doi.org/10.1016/j.jfca.2003.10.008

Attia, Y. A., Abd El-Hamid, A. E., Bovera, F., Al-Sayed, M. I. 2009. Reproductive and productive performance of rabbit does submitted to an oral glucose supplementation. An international Journal of Animal Bioscience, vol. 3, no. 10, p. 1401-1407. PMid:22444934

Baltrušaityté, V., Venskutonis, P. R., Čkstaryté, V. 2007. Radical scavenging activity of different floral origin honey and beebread phenolic extracts. Food Chemistry, vol. 101, no. 2, p. 502-514. https://doi.org/10.1016/j.foodchem.2006.02.007

Bentolila, V., Boyce, T. M., Fyhrie, D. P., Drumb, R., Skerry, T. M., Schaffler, M. B. 1998. Intracortical remodeling in adult rat long bones after fatigue loading. Bone, vol. 23, no. 3, p. 275-281. https://doi.org/10.1016/S8756-3282(98)00104-5 PMid:9737350

Brandi, M. L., Collin-Osdoby, P. 2006. Vascular biology and the skeleton. Journal of Bone and Mineral Research, vol. 21, no. 2, p. 183-192. https://doi.org/10.1359/JBMR.050917 PMid:16418774

D'Haese, P. C., Couttenye, M. M., Lamberts, L. V., Elseviers, M. M., Goodman, W. G.,. Schrooten, I., Cabrera, W. E., De Broe, M. E. 1999. Aluminium, iron, lead, cadmium, copper, zinc, chromium, magnesium, strontium, and calcium content in bone of end-stage renal failure patients. Clinical Chemistry, vol. 45, no. 9, p. 1548-1556. PMid:10471660

Eghbali-Fatourechi, G. Z., Lamsam, J., Fraser, D., Nagel, D., Riggs, B. L., Khosla, S. 2005. Circulating osteoblast-lineage cells in humans. The New England Journal of Medicine, vol. 352, no. 19, p. 1959-1966. PMid:15888696

Enlow, D. H., Brown, S. O. 1956. A comparative histological study of fossil and recent bone tissues. Part I. Texas Journal of Science, vol. 8, p. 405-412.

Erben, R. G. 1996. Trabecular and endocortical bone surfaces in the rat: modeling or remodeling? Anatomical Record, vol. 246, no. 1, p. 39-46. PMid:8876822

Estevinho, L., Moreira, L., Dias, G., Pereira, E. 2008. Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugam honey. Food and Chemical Toxicology, vol. 46, no. 12, p. 3774-3779. https://doi.org/10.1016/j.fct.2008.09.062 PMid:18940227

Estevinho, L. M., Rodrigues, S., Pereira, A. P ., Feás, X., 2012. Portuguese bee pollen: palynological study nutritional and microbiological evaluation. International Journal of Food Science and Technology, vol. 47, no. 2, p. 429-435. https://doi.org/10.1111/j.1365-2621.2011.02859.x

Gálik, B. 2012. Vplyv fytogénnych kŕmnych aditív na využiteľnosť živín u neprežúvavcov. (Phytogenic feed additives influence the usability of nutrients in non-ruminants.) Habilitation thesis. SUA Nitra, p. 47-56.

Gergen, I., Radu, F., Bordean, D., Isengard, H. I. 2006. Determination of water content in bee's pollen samples by Karl Fischer titration. Food Control, vol. 17, no. 3,

p. 176-179. https://doi.org/10.1016/j.foodcont.2004.09.018

Gozálbez, F. 1984. El polen apícola español. Composición botánica y características fisicoquímicas" El Campo del Banco Bilbao. Apicultura, vol. 93, p. 53-60.

Hamamoto, R., Ishiyama, K., Yamaguchi, M. 2006. Inhibitory effects of bee pollen Cistus ladaniferus extract on bone resorption in femoral tissues and osteoclast-like cell formation in bone marrow cells in vitro. Journal of Health Science, vol. 52, no. 3, p. 268-275. https://doi.org/10.1248/jhs.52.268

Haro, A., Lopez-Alliaga, I., Lisbona, F., Barrionuevo, M., Alferez, M. J. M., Campos, M. S. 2000. Beneficial effect of pollen and/or propolis on the metabolism of iron, calcium, phosphorus, and magnesium in rats with nutritional ferropenic anemia. Journal of Agricultural and Food Chemistry, vol. 48, no. 11, p. 5715-5722. https://doi.org/10.1021/jf000635h PMid:11087544

Haščík, P., Eliman, I. E., Bobko, M., Kačániová, M., Pochop, J., Garlik, J., Kročko, M., Čuboň, J., Vavrišínová, K., Arpášová, H., Capcarová, M., Benczová, M. 2011. Oxidative stability of chicken meat after pollen extract application in their diet. Journal of Microbiology, Biotechnology and Food Sciences, vol. 1, p. 70-82. [cit. 2014-07-10] Available at: http://www.jmbfs.org/issue/august-september-2011-vol-1-no-1/jmbfs_0007_hascik-et-al.-2011/?issue_id=389&article_id=4

Ibbotson, K. J., Orcutt, C. M., D'Souza, S. M., Paddock, C. L., Arthur, J. A., Jankowsky, M. L., Boyce, R. W. 1992. Contrasting effects of parathyroid hormone and insulin-like growth factor I in an aged ovariectomized rat model of postmenopausal osteoporosis. Journal of Bone and Mineral Research, vol. 7, no. 4, p. 425-432. https://doi.org/10.1002/jbmr.5650070410 PMid:1609630

Kassem, M., Risteli, L., Mosekilde, L., Melsen, F., Eriksen, E. F. 1991. Formation of osteoblast-like cells from human mononuclear bone marrow cultures. Acta pathologica, microbiologica, et immunologica Scandinavica, vol. 99, no. 3, p. 269-274. PMid:2018640

Katsumata, S., Katsumata-Tsuboi, R., Uehara, M., Suzuki, K. 2009. Severe iron deficiency decreases both bone formation and bone resorption in rats. Journal of Nutrition, vol. 139, no. 2, p. 238-243. https://doi.org/10.3945/jn.108.093757 PMid:19106323

Lauritzen, D. B., Balena, R., Shea, M., Seedor, J. G., Markatos, A., Le, H. M., Toolan, B. C., Myers, E. R., Rodan, G. A., Hayes, W. C. 1993. Effects of combined prostaglandin and alendronate treatment on the histomorphometry and biomechanical properties of bone in ovariectomized rats. Journal of Bone and Mineral Research, vol. 8, no. 7, p. 871-879. https://doi.org/10.1002/jbmr.5650080713 PMid:8352069

Martiniaková, M., Omelka, R., Grosskopf, B., Sirotkin, A. V., Chrenek, P. 2008. Sex-related variation in compact bone microstructure of the femoral diaphysis in juvenile rabbits. Acta Veterinaria Scandinavica, vol. 50, p. 15. https://doi.org/10.1186/1751-0147-50-15 PMid:18522730

Martiniaková, M., Omelka, R., Jančová, A., Stawarz, R., Formicki, G. 2010. Heavy metal content in the femora of yellow-necked mouse (Apodemus flavicollis) and wood mouse (Apodemus sylvaticus) from different types of polluted environment in Slovakia. Environmental Monitoring and Assessment, vol. 171, no. 1-4, p. 651-660. https://doi.org/10.1007/s10661-010-1310-1 PMid:20135219

Martiniaková, M., Omelka, R., Jančová, A., Stawarz, R., Formicki, G. 2011. Concentrations of selected heavy metals in bones and femoral bone structure of bank (Myodes glareolus) and common (Microtus arvalis) voles from different polluted biotopes in Slovakia. Archives of Environmemtal Contamination and Toxicology, vol. 60, no. 3, p. 524-532. https://doi.org/10.1007/s00244-010-9545-y PMid:20532880

Oliveira, M. C., Silva, D. M., Loch, F. C., Martins, P. C., Dias, D. M. B., Simon, G. A. 2013. Effect of bee pollen on the immunity and tibia characteristics in broilers. Brazilian Journal of Poultry Science, vol. 15, p. 323-328.

[cit. 2014-07-10] Available at: http://www.scielo.br/pdf/rbca/v15n4/v15n4a06.pdf

Orzaez Villanueva, M. T., Diaz Marquina, A., Bravo Serrano, R., Blazquez Abelian, G. 2002. The importance of bee-collected pollen in the diet: a study of its composition. International Journal of Food Sciences Nutrition, vol. 53, no. 3, p. 217-224. PMid:11951585

O'Neil, C. K., Evans, E. 2004. Beyond calcium and vitamin D. Clinical Reviews in Bone Mineralisation and Metabolism, vol. 2, no. 4, p. 325-339. https://doi.org/10.1385/BMM:2:4:325

Pernal, S. F., Currie, R. W. 2002. Discrimination and preferences for pollen-based cues by foraging honeybees, Apis mellifera L. Animal Behaviour, vol. 63, p. 369-390.https://doi.org/10.1006/anbe.2001.1904

Petruška, P., Tušimová, E., Kalafová, A., Haščík, P., Kolesárová, A., Capcarová, M. 2012. Effect of propolis in chicken diet on selected parameters of mineral profile. Journal of Microbiology, Biotechnology and Food Sciences, vol. 1, p. 593-600.

Reim, N. S., Breig, B., Stahr, K., Eberle, J., Hoeflich, A., Wolf, E., Erben, R. G. 2008. Cortical bone loss in androgen-deficient aged male rats is mainly caused by increased endocortical bone remodeling. Journal of Bone and Mineral Research, vol. 23, p. 694-704. PMid:18433303

Ricqlès, A. J., Meunier, F. J., Castanet, J., Francillon-Vieillot, H. 1991. Comparative microstructure of bone. Bone 3, Bone Matrix and Bone Specific Products. Hall BK. Boca Raton: CRC Press; p. 1-78. ISBN 0-8493-8823-6.

Roulston, T. H., Cane, J. H. 2000. Pollen nutritional content and digestibility for animals. Plant Systematics and Evolution, vol. 222, p. 187-209. [cit. 2014-07-10] Available at: http://www.inkcorrosion.org/reports/000592/front.pdf

Stawarz, R., Zakrzewski, M., Marenčík, A., Hraška, Š. 2003. Heavy metal concentration in the toad Bufo Bufo from a region of Mochovce, Slovakia. Ekológia Bratislava, vol. 22, p. 292-297.

Uzawa, T., Hori, M., Ejiri, S., Ozawa, H. 1995. Comparison of the effects of intermittent and continuous administration of human parathyroid hormone (1-34) on rat bone. Bone, vol. 16, no. 4, p. 477-484. PMid:7605709

Wang, J., Li, S., Wang, Q., Xin, B., Wang, H. 2007. Trophic effect of bee pollen on small intestine in broiler chickens. Journal of Medicinal Food, vol. 10, p. 276-280. https://doi.org/10.1089/jmf.2006.215 PMid:17651063

Weber, K., Kaschig, C., Erben, R.G. 2004. 1 Alpha-Hydroxyvitamin D2 and 1 alpha-hydroxyvitamin D3 have anabolic effects on cortical bone, but induce intracortical remodeling at toxic doses in ovariectomized rats. Bone,

vol. 35, no. 3, p. 704-710. https://doi.org/10.1016/j.bone.2004.04.011 PMid:15336607

Yamaguchi, M., Inamoto, K., Suketa, Y. 1986. Effect of essential trace metals on bone metabolism in weanling rats: comparison with zinc and other metals' actions. Resarch in Experimental Medicine, vol. 186, no. 5, p. 337-342. PMid:3797821

Yamaguchi, M., Igarashi, A., Uchiyama, S., Morita, S., Sugawara, K., Sumida, T. 2004. Prolonged intake of juice (Citrus unshiu) reinforced with β-cryptoxanthin has an effect on circulating bone biochemical markers in normal individuals. Journal of Health Science, vol. 50, no. 6, p. 619-624. https://doi.org/10.1248/jhs.50.619

Yamaguchi, M., Hamamoto, R., Uchiyama, S., Ishiyama, K., Hashimoto, K. 2006. Anabolic effects of bee pollen Cistus ladaniferus extract on bone components in the femoral diaphyseal and metaphyseal tissues of rats in vitro and in vivo. Journal of Health Science, vol. 52, p. 43-49. https://doi.org/10.1248/jhs.52.43

Zuo, J., Xu, S. 2003. Study and application of bee pollen as feed additive. Feed Review, vol. 11, p. 33-35.

Downloads

Published

2014-11-17

How to Cite

Martiniaková, M. ., Boboňová, I. ., Omelka, R. ., Ďúranová, H. ., Babosová, R. ., Stawarz, R. ., & Toman, R. . (2014). Low administration of bee pollen in the diet affects qualitative histological characteristics of bone in male rats. Potravinarstvo Slovak Journal of Food Sciences, 8(1), 277–283. https://doi.org/10.5219/396

Most read articles by the same author(s)

<< < 1 2