Immunofluorescent determination of wheat protein in meat products


  • Michaela Petrášová University of veterinary and Pharmaceutical Scientis Brno, Faculty of Veterinary Hygiene and Ecology, Vegetable Foodstuffs Hygiene and Technology, Palackého tř. 1/3, 612 42 Brno
  • Eliška Zichová University of veterinary and Pharmaceutical Scientis Brno, Faculty of Veterinary Hygiene and Ecology, Vegetable Foodstuffs Hygiene and Technology, Palackého tř. 1/3, 612 42 Brno
  • Matej Pospiech University of veterinary and Pharmaceutical Scientis Brno, Faculty of Veterinary Hygiene and Ecology, Vegetable Foodstuffs Hygiene and Technology Palackého tř. 1/3, 612 42 Brno
  • Bohuslava Bohuslava Tremlová University of veterinary and Pharmaceutical Scientis Brno, Faculty of Veterinary Hygiene and Ecology, Vegetable Foodstuffs Hygiene and Technology Palackého tř. 1/3, 612 42 Brno



fluorescence microscopy, plant allergens, meat, addulteration, celiac deases


In food industry nowadays, there are various plant-origin protein additives which are meant for production of meat products. Among the most frequent additives of this type there are different kinds of flour, starch, fiber, and plant-origin proteins. Their usage at present is limited by the existing legislation not to prevent consumer deception but also for reasons of possible influence on consumer health. Therefore, this problem is paid a lot of attention not only in the Czech Republic but also all over the world. The main risk is seen in the impossibility to choose a suitable foodstuff for an individual prone to allergic reactions. Potential allergens are also often plant-origin raw materials which are added into foodstuffs for their technological qualities and low price. Wheat is widely cultivated cereal as well as an important source of proteins. After ingestion or inhalation, wheat proteins may cause adverse reactions. These adverse effects include a wide range of disorders which are dependent on the method of contact with wheat protein. These adverse effects can then take the form of various clinical manifestations, such as celiac disease, T-cell mediated inflammatory bowel disease, dermatitis, skin rash, breathing difficulties, allergy to pollen or to wheat flour or food allergy to foodstuffs containing gluten. The only possible protection against adverse immune reactions for those with food allergies is strictly excluding the allergen from their diet. Although the number of studies dealing with the reduction or loss of allergenicity is increasing, yet these practices are not common. Most of the population suffering from food allergies is thus still dependent on strict exclusion of foodstuffs causing adverse allergic reactions from their diet. In order to avoid misleading consumers and also to protect allergic consumers, analytical methods applicable to all types of foodstuffs have been developed. Unfortunately, detection of allergens in foodstuffs is relatively difficult because of the fact that they occur in trace amounts and are often masked by various parts of the product. This paper deals with detection of wheat protein in meat products bought in the retail network of the Czech Republic. Ten cooked meat products, especially types of sausages and soft salami which stated wheat protein in their composition, were examined. The samples were processed using the method of immunofluorescence and stained with Texas Red fluorochrome. The presence of wheat protein was demonstrated in all the examined meat products. From the results it follows that the method of immunofluorescence is suitable for detection of wheat protein in meat products.


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Aalberse, R. C., Akkerdass, J., Van Ree, R. 2001. Cross-reactivity of IgE antibodies to allergens. Allergy, vol. 56, no. 6, p. 478-490. PMid:11421891 DOI:

Battais, F., Richard, C., Jacquenet, S., Denery-Papini, S., Moneret-Vautrin, D. A. 2008. Wheat grain allergies: an update on wheat allergens. European Annals of Allergy and Clinical Immunology, vol. 40, no. 3, p. 67-76. PMid:19334370

Baur, X. 1999. Baker's asthma: causes and prevention. International Archives of Occupational and Environmental Health, vol. 72, no. 5, p. 292-296. PMid:10447658 DOI:

Brujinzell-Koomen, C., Ortolani, C., Aas, K., Bindslev-Jensen, C., Bjorksten, B., Moneret-Vautrin, D., Wutrich, B. 1995. Adverse reactions to food. Allergy, European Academy of Allergology and Clinical Immunology. vol. 50, no. 8, p. 623-625. DOI:

Ferguson, A. 1992. Definitions and diagnosis of food intolerance and food allergy: consensus and controversy. Journal of Pediatrics, vol. 121, no. 5, p. 7-11. DOI:

Fu, T. J., Abbott, U. R., Hatzos, C. 2002. Digestibility of food allergens and nonallergenic proteins in stimulated gastric fluid and stimulated intestinal fluid: a comparative study. Journal of Agricultural and Food Chemistry, vol. 50, no. 24, p. 7154-7160. PMid:12428975 DOI:

Hefle, S. L., Nordlee, J. A., Taylor, S. L. 1996. Allergenic foods. Critical Reviews in Food Science and Nutrition, vol. 36, p. S69-S89. PMid:8959379 DOI:

Hischenhuber, C., Crevel, R., Jarry, B. 2006. Review article: safe amounts of gluten for patients with wheat allergy or coeliac disease. Alimentary Pharmacology & Therapeutics, vol. 23, no. 5, p. 559-575. PMid:16480395 DOI:

Lehto, M., Palosuo, K., Varjonen, E. 2003. Humoral and cellular responses to gliadin in wheat-dependent, exercise-induced anaphylaxis. Clinical & Experimental Allergy, vol. 33, no. 1, p. 90-95. PMid:12534555 DOI:

Moneret-Vautrin, D. A., Kanny, G., Guerin, L., Flabbee, J., Lemerdy, P. 2000. The multifood allergy syndrome. Journal of Allergy and Clinical Immunology (Paris), vol. 32, no. 1, p. 12-15. PMid:10723530

Moneret-Vautrin, D. A., Kanny, G., Périer, P. 2003. Prospective study (199-2002) of wheat flour allergy in children and adults, with reference to celiac disease. Relationship of DR1 allele to allergy in children. Alim. Inter, vol. 8, p. 2-8. DOI:

Moneret-Vautrin, D. A., Morisset, M., Flabbee, J., Beaudouin, E., Kanny, G. 2005. Epidemiology of life-threatening and lethal anaphylaxis: a review. Allergy, vol. 60, no. 4, p. 443-451. PMid:15727574 DOI:

Niggemann B. 2001. The role of the atopy patch test (APT) in diagnosis of food allergy in infants and children with atopic dermatitis. Pediatric Allergy and Immunology, vol. 12, no. 14, p. 37-40. PMid:11380896 DOI:

Pourpak, Z., Ghojezadeh, L., Mansouri, M., Mozaffari, H., Farhoudi, A. 2007. Wheat anaphylaxis in children. Immunological investigations, vol. 36, no. 2, p. 175-182. PMid:17365018 DOI:

Rasanen, L., Lehto, M., Turjanmaa, K., Savolainen, J., Reunala, T. 1994. Allergy to ingested cereals in atopic children. Allergy, vol. 49, no. 10, p. 871-876. PMid:7535982 DOI:

Rance, F., Grandmottet, X., Grandjean, H. 2005. Prevalence and main characteristics of schoolchildren diagnosed with food allergies in France. Clin. Exp. Allergy, vol. 35, no. 2, p. 167-172. PMid:15725187 DOI:

Rance, F., Kanny, G., Dutau, G., Moneret-Vautrin, D. 1998. Aspects cliniques de l'allergie alimentaire. Rev. Fr. Allergol, vol. 38, p. 900-905. DOI:

Regulation (EC) No 2000/13 of 20 March 2000 on the approximation of the laws of Member States relating to the labeling, presentation and advertising of foodstuffs. OJ L 109, 06.05.2000, p. 0029-0042.

Regulation (EC) No 2003/89 of 10 November 2003 amending Regulation 2000/13/EC as regards indication of the ingredients present in foodstuffs (Text with EEA relevance). OJ L 308, 25.11.2003, p. 0015-0018.

Regulation (EC) No 2006/142 of 22 December 2006 amending Annex IIIa to Directive of the European Parliament and Council regulation 2000/13/EC, listing the ingredients which must under all circumstances appear on the labeling of foodstuffs (Text with EEA relevance). OJ L 368, 23.12.2006, p. 110-111.

Scibilia, J., Pastorello, E. A., Zisa, G. 2006. Wheat allergy: a doubleblind, placebo-controlled study in adults. Journal of Allergy and Clinical Immunology, vol. 117, no. 2, p. 433-439. PMid:16461145 DOI:

Sicherer, S. H. 2000. Determinants of systemic manifestations of food allergy. Journal of Allergy and Clinical Immunology, vol. 106, no. 5, p. S251-257. PMid:11080740 DOI:

Sicherer, S. H., Morrow, E. H., Sampson, H. A. 2000. Dose-response in double-blind, placebo-controlled oral food challenges in children with atopic dermatitis. Journal of Allergy and Clinical Immunology, vol. 105, no. 3, p. 582-586. PMid:10719311 DOI:

Sicherer, S. H., Sampson, H. A. 2000. Peanut and tree nut allergy. Current Opinion in Pediatrics, vol. 12, no. 6, p. 567-573. PMid:11106277 DOI:

Talandová, M., Zichová, E., Pospiech, M., Tremlová, B. 2013. Využití fluorescenční mikroskopie k detekci pšeničného proteinu v modelových masných výrobcích. Mladí vedci. Bratislava: Ministerstvo pôdohospodárstva a rozvoja vidieka SR, p. 114. ISBN-978-80-970552-8-8.




How to Cite

Petrášová, M. ., Zichová, E. ., Pospiech, M. ., & Bohuslava Tremlová, B. . (2014). Immunofluorescent determination of wheat protein in meat products. Potravinarstvo Slovak Journal of Food Sciences, 8(1), 82–86.

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