Incorporation of catechin extracts from gambier products and pasak bumi in the production of functional instant green robusta coffee


  • Budi Santoso Sriwijaya University: Ogan Ilir, Agricultural Technology Department, Faculty of Agriculture, South Sumatera, Indonesia, Tel.: +628127853631
  • Muhammad Ridho Wahyu Aulia Sriwijaya University: Ogan Ilir, Agricultural Technology Department, Faculty of Agriculture, South Sumatera, Indonesia, Tel.: +6281377937776
  • Syerina Raihatul Jannah Sriwijaya University: Ogan Ilir, Agricultural Technology Department, Faculty of Agriculture, South Sumatera, Indonesia, Tel.: +6282175800458
  • Gatot Priyanto Sriwijaya University: Ogan Ilir, Agricultural Technology Department, Faculty of Agriculture, South Sumatera, Indonesia, Tel.: +6281233463906
  • Agus Wijaya Sriwijaya University: Ogan Ilir, Agricultural Technology Department, Faculty of Agriculture, South Sumatera, Indonesia, Tel:+6281377844401
  • Hermanto Hermanto Sriwijaya University: Ogan Ilir, Agricultural Technology Department, Faculty of Agriculture, South Sumatera, Indonesia, Tel.: +6281379133523



gambier, instant, catechin, green coffee, pasak bumi


The research was used to produce functional instant green coffee through gambier catechin extract and pasak bumi powder. This involved using a non-factorial completely randomized design with 5 treatments and 3 replications. The treatments consist of 5 formulations (F), including the instant green coffee (%), gambir catechin extract (%), and pasak bumi powder (%) where F1 was at 100:0:0, F2 was 80:15:5, F3 was 70:20:10, F4 was 60:25:15, and F5 was 50:30:20. The results showed the functional instant green coffee produced has a water content of 3.84 – 4.81%, soluble speed of 26.78 – 29.33 seconds, and total phenol of 16.79 – 169.48 mg/L, and IC50 of 44.68- 207.59 ppm. The addition of gambier catechin extract and pasak bumi powder to the formulation was observed to have significantly increased the functional properties and water content. Moreover, the soluble speed of the instant coffee fulfils the quality requirements of the Indonesian National Standard (SNI) number 2983 of 2014.


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Saeed Alkaltham, M., Musa Özcan, M., Uslu, N., Salamatullah, A. M., & Hayat, K. (2020). Effect of microwave and oven roasting methods on total phenol, antioxidant activity, phenolic compounds, and fatty acid compositions of coffee beans. In Journal of Food Processing and Preservation (Vol. 44, Issue 11). Wiley. DOI:

AOAC. 2012. Official Methods of Analysis (18th edition) Association of Official Analytical. Chemists International. Maryland, USA.

Bobková, A., Hudáček, M., Jakabová, S., Belej, Ľ., Capcarová, M., Čurlej, J., Bobko, M., Árvay, J., Jakab, I., Čapla, J., & Demianová, A. (2020). The effect of roasting on the total polyphenols and antioxidant activity of coffee. In Journal of Environmental Science and Health, Part B (Vol. 55, Issue 5, pp. 495–500). Informa UK Limited. DOI:

Christianty, F. M., Holidah, D., Fajrin, F. A., Salsabina, M. C. A., & Roni, A. (2020). The Lipid Profile and Aorta Histopathology on Hyperlipidemic Rat by Giving Green Coffee Extract. In Indonesian Journal of Pharmaceutical Sciences (Vol. 18, Issue 1, pp. 21–27). Universitas Gadjah Mada - Faculty of Pharmacy.

Cheng, K., Dong, W., Long, Y., Zhao, J., Hu, R., Zhang, Y., & Zhu, K. (2019). Evaluation of the impact of different drying methods on the phenolic compounds, antioxidant activity, and in vitro digestion of green coffee beans. In Food Science & Nutrition (Vol. 7, Issue 3, pp. 1084–1095). Wiley. DOI:

Górnaś, P., Dwiecki, K., Siger, A., Tomaszewska-Gras, J., Michalak, M., & Polewski, K. (2015). Contribution of phenolic acids isolated from green and roasted boiled-type coffee brews to total coffee antioxidant capacity. In European Food Research and Technology (Vol. 242, Issue 5, pp. 641–653). Springer Science and Business Media LLC. DOI:

Haile, M., & Kang, W. H. (2020). Antioxidant Properties of Fermented Green Coffee Beans with Wickerhamomyces anomalus (Strain KNU18Y3). In Fermentation (Vol. 6, Issue 1, p. 18). MDPI AG. DOI:

Herawati, D., Giriwono, P. E., Dewi, F. N. A., Kashiwagi, T., & Andarwulan, N. (2018). Critical roasting level determines bioactive content and antioxidant activity of Robusta coffee beans. In Food Science and Biotechnology (Vol. 28, Issue 1, pp. 7–14). Springer Science and Business Media LLC. DOI:

Ibtisam, K., & Karim. (2013). Optimization of instan controlled pressure drop dic-assisted solvent extraction of total phenols of green coffee beans. In Journal of Food Studies (Vol. 2, Issue 1, pp. 42-61). Macrothink Institute.

Iriawati, Rahmawati, A., & Esyanti, R. R. (2014). Analysis of Secondary Metabolite Production in Somatic Embryo of Pasak Bumi (Eurycoma Longifolia Jack.). In Procedia Chemistry (Vol. 13, pp. 112–118). Elsevier BV. DOI:

Ismail, A. S., Rizal, Y., Armenia, A., & Kasim, A. (2021). Determination of the best method for processing gambier liquid by-product [Uncaria gambir (hunter) roxb] as natural antioxidant sources. In Journal of the Indonesian Tropical Animal Agriculture (Vol. 46, Issue 2, pp. 166–172). Institute of Research and Community Services Diponegoro University (LPPM UNDIP). DOI:

Isnindar, Wahyuono, S., & Widyarini, S. (2017). The antioxidant activity of Merapi green coffee berries. In Journal of Pharmaceutical Science and Clinical Research (Vol. 2, Issue 2, pp. 130–136). DOI:

Khanam, Z., Wen, C. S., & Bhat, I. U. H. (2015). Phytochemical screening and antimicrobial activity of root and stem extracts of wild Eurycoma longifolia Jack (Tongkat Ali). In Journal of King Saud University - Science (Vol. 27, Issue 1, pp. 23–30). Elsevier BV. DOI:

Kuncoro, S., Sutiarso, L., Karyadi, J. N. W., & Masithoh, R. E. (2018). Kinetika Reaksi Penurunan Kafein dan Asam Klorogenat Biji Kopi Robusta melalui Pengukusan Sistem Tertutup. In Agritech (Vol. 38, Issue 1, p. 105). Universitas Gadjah Mada. DOI:

Ko, B. S., Lim, S. H., & Han. S. H. (2017). Quality Characteristics of Instant Coffee with Probiotics and Microground Coffee. In Culinary Science and Hospitality Research (Vol. 23, Issue 8, pp. 153–162). DOI:

Lelyana, R. & Cahyono, B. (2015). Total phenolic acid contents in some commercial brands of coffee from Indonesia. In Journal of Medical Plant and Herbal Therapy Research (Vol. 3, pp. 27–29). BluePen Journals.

Maesaroh, K., Kurnia, D., & Al Anshori, J. (2018). Perbandingan Metode Uji Aktivitas Antioksidan DPPH, FRAP dan FIC Terhadap Asam Askorbat, Asam Galat dan Kuersetin. In Chimica et Natura Acta (Vol. 6, Issue 2, p. 93). Universitas Padjadjaran. DOI:

Masek, A., Latos-Brozio, M., Kałużna-Czaplińska, J., Rosiak, A., & Chrzescijanska, E. (2020). Antioxidant Properties of Green Coffee Extract. In Forests (Vol. 11, Issue 5, p. 557). MDPI AG. DOI:

Matarani, F., Mursalin, & Gusriani, I. (2019). The effect of increasing the concentration of maltodextrin on the quality of instant coffee from robusta coffee grounds using a vacuum dryer. In Proceedings of SEMIRATA BKS-PTN West Region in the Field of Agricultural Science (Vol. 1, Issue 1, pp. 922-941).

Melia, S., Novia, D., & Juliyarsi, I. (2015). Antioxidant and Antimicrobial Activities of Gambir (Uncaria gambir Roxb) Extracts and Their Application in Rendang. In Pakistan Journal of Nutrition (Vol. 14, Issue 12, pp. 938–941). Science Alert. DOI:

Mursalin, M., Nizori, A., & Rahmayani, I. (2019). The Effect of Heating Schedule on Physico-Chemical Properties of Instant Coffee of Liberika Tungkal Jambi. In Indonesian Food Science & Technology Journal (Vol. 2, Issue 2, pp. 26–29). Faculty of Education and Teacher Training, Jambi University. DOI:

Nichmah, L., Yuwanti, S., & Suwasono, S. (2019). Kopi kayu manis celup dengan variasi tingkat penyangraian kopi dan konsentrasi bubuk kayu manis. In Berkala Ilmiah Pertanian (Vol. 2, Issue 2, p. 50). UPT Penerbitan Universitas Jember. DOI:

Odžaković, B., Džinić, N., Kukrić, Z., & Grujić, S. (2016). Effect of roasting degree on the antioxidant activity of different Arabica coffee quality classes. In Acta Scientiarum Polonorum Technologia Alimentaria (Vol. 15, Issue 4, pp. 409–417). Uniwersytet Przyrodniczy w Poznaniu (Poznan University of Life Sciences). DOI:

Pambayun, R., Gardjito, M., Sudarmadji, S., & Kuswanto, K. R. 2007. Phenolic content and antibacterial properties of various extracts of gambir (Uncaria gambir Roxb). In Indonesian Journal of Pharmacy (Vol. 18, Issue 3, pp. 141-146). Faculty of Pharmacy Universitas Gadjah Mada.

Vareltzis, P. K., Zeleskidou, M., Kiroglou, S., & Gargali, I. (2020). Production of instant coffee from cold brewed coffee; process characteristics and optimization. In Food Science and Applied Biotechnology (Vol. 3, Issue 1, p. 39). University of Food Technologies - Plovdiv. DOI:

Pranowo, D., Adiatmi, A. Y., & Dewi, I. A. (2021). Production optimization of green coffee extracts from Jember robusta (Coffeacanephora) coffee using foam mat drying method. In IOP Conference Series: Earth and Environmental Science (Vol. 733, Issue 1, p. 012100). IOP Publishing. DOI:

Pranowo, D., Perdani, C. G., Prihardhini, T. A., Wijana, S., Fahmi, A. S. & Arisandi, D. M. (2020). Optimization of microencapsulation process of green coffee extract with spray drying as a dietary supplement. In Systematic Reviews in Pharmacy (Vol. 11, Issue 10, pp. 715–721). Wolters Kluwer Medknow Publications.

Praptiningsih Y. S., Tamtarini, I., & Wijayanti, S. (2012). The properties of coconut sugar instant coffee from various ratios of Arabica Robusta coffee and coconut sugar to granulated sugar. In Journal of Agrotechnology (Vol. 6, Issue 1, pp. 70–77). OMICS Publishing Group.

Rahmawati, N., & Wachyuni, A. F. (2013). Kandungan fenolik dan aktivitas antioksidan ekstrak daun gambir kering (Uncaria gambir (Hunter) Roxb.). In Jurnal Indonesian Chimica Acta (Vol. 4, pp. 1–6). Hasanuddin University.

Samsonowicz, M., Regulska, E., Karpowicz, D., & Leśniewska, B. (2019). Antioxidant properties of coffee substitutes rich in polyphenols and minerals. In Food Chemistry (Vol. 278, pp. 101–109). Elsevier BV. DOI:

Santoso, B., Hazirah, R., Priyanto, G., Hermanto, D. & Sugito, D. (2019). Utilization of Uncaria gambir Roxb filtrate in the formation of bioactive edible films based on corn starch. In Food Science and Technology (Vol. 39, Issue 4, pp. 837–842). FapUNIFESP (SciELO). DOI:

Septiani, Dewi, E. N., & Wijayanti, I. (2007). Antibacterial activity of seagrass extract (Cymodocea rotundata) against Staphyloccus and Escherichia coli bacteria. In Fisheries Saintek (Vol.13, Issue 1, pp. 1–6). DOI:

SNI 2983 : 2014. Instant coffee. National Standardization Board. Jakarta.

Siva, R. (2016). Assessment Of Antioxidant Activity And Total Phenolic Content From Green Coffee Robusta Sp. BEANS. In Malaysian Journal of Analytical Science (Vol. 20, Issue 5, pp. 1059–1065). Penerbit Universiti Kebangsaan Malaysia (UKM Press). DOI:

Jeszka-Skowron, M., Frankowski, R., & Zgoła-Grześkowiak, A. (2020). Comparison of methylxantines, trigonelline, nicotinic acid and nicotinamide contents in brews of green and processed Arabica and Robusta coffee beans – Influence of steaming, decaffeination and roasting processes on coffee beans. In LWT (Vol. 125, p. 109344). Elsevier BV. DOI:

Tasew, T., Mekonnen, Y., Gelana, T., Redi-Abshiro, M., Chandravanshi, B. S., Ele, E., Mohammed, A. M., & Mamo, H. (2020). In Vitro Antibacterial and Antioxidant Activities of Roasted and Green Coffee Beans Originating from Different Regions of Ethiopia. In International Journal of Food Science (Vol. 2020, pp. 1–8). Hindawi Limited. DOI:

Triawanti, Sanyoto, D. D., & Noor, M. S. (2020). The supplementation of pasak bumi (Eurycoma longifolia Jack.) in undernourished rats to increase spatial memory through antioxidant mechanism. In Clinical Nutrition Experimental (Vol. 33, pp. 49–59). Elsevier BV. DOI:

Widiyarti, G., A. Sundowo, E. Filailla, and J.A. Laksmono. (2020). The mechanically extraction process from leaves and twigs of gambier (Uncaria gambier Roxb) and its antioxidant activity. In Journal of Pure and Applied Chemistry Research (Vol. 9, Issue 1, pp. 18–15. DOI:

Wolska, J., Janda, K., Jakubczyk, K., Szymkowiak, M., Chlubek, D., & Gutowska, I. (2017). Levels of Antioxidant Activity and Fluoride Content in Coffee Infusions of Arabica, Robusta and Green Coffee Beans in According to their Brewing Methods. In Biological Trace Element Research (Vol. 179, Issue 2, pp. 327–333). Springer Science and Business Media LLC. DOI:

Yeni, G., Syamsu, K., Mardliyati, E., & Muchtar, H. (2017). Determination of the process technology for making pure gambier and standardized catechins from random gambier. In Industrial Research and Development Journal (Vol. 7, Issue 1, pp 1–10). Technicals Publications. DOI:




How to Cite

Santoso, B., Muhammad Ridho Wahyu Aulia, Syerina Raihatul Jannah, Gatot Priyanto, Agus Wijaya, & Hermanto Hermanto. (2022). Incorporation of catechin extracts from gambier products and pasak bumi in the production of functional instant green robusta coffee. Potravinarstvo Slovak Journal of Food Sciences, 16, 367–374.