Genetic identification of the causative agent of Brucellosis

Yerkebulan Jakipov; Muafik Mustafin; Anda Valdovska; Sayat Baiseitov; Ayauly Aitkulova

doi:10.5219/1664

Authors

Yerkebulan Jakipov Akhmet Baitursynov Kostanay State University, Department of Veterinary Medicine, Baytursynov Str., 47A, 110000, Kostanay, Republic of Kazakhstan, Tel.: +7 (7142) 558463 https://orcid.org/0000-0002-9747-9411
Muafik Mustafin Akhmet Baitursynov Kostanay State University, Department of Veterinary Medicine, Baytursynov Str., 47A, 110000, Kostanay, Republic of Kazakhstan, Tel.: +7 (7142) 558488
Anda Valdovska Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Liela Str., 2, LV-3001, Jelgava, Latvia, Tel.: +371 630 24662
Sayat Baiseitov Omsk State Agrarian University named after P.A. Stolypin, Department of Veterinary Microbiology, Infectious and Invasive Diseases, Institutskaya Sq., 1, 644008, Omsk, Russian Federation, Tel.: +7 (3812) 25-05-70
Ayauly Aitkulova Kazakh National Agrarian University, Department of Biological Safety, Abai Ave., 8, 050010, Almaty, Republic of Kazakhstan, Tel.: +7 (727) 261-5857

DOI:

https://doi.org/10.5219/1664

Keywords:

amplification, sequencing, Brucella abortus, Brucella melitensis, strain

Abstract

The development of animal husbandry suffers various kinds of losses due to the spread of infectious diseases among animals, in particular Brucellosis. A challenge faced by Brucella researchers has been the accurate identification of new isolates within the genus while preserving sufficient, and not excessive, biosafety and biosecurity requirements. The availability of discriminatory molecular typing tools to inform and assist conventional epidemiological approaches would be invaluable in controlling these infections, but efforts have been hampered by the genetic homogeneity of the genus. In this work, for better identification of infection, for control and monitor the source of outbreaks in prosperous areas was carried out identification of Brucella spp. strains which circulating in the Kostanay region. For this was used using multilocus analysis of a variable number of tandem repeats sequenced by 16 s – PNK on a genetic analyzer (sequencer). According to the results of a study of cattle, cultures of microorganisms were infected: No. 4, 5, 7, 8. Comparison of the obtained results with similar results of domestic and foreign works by A. Shevtsov, G. Borrello, P. Le Fleche, G. Garofolo suggest that the genotyping of local strains has an importance in the molecular epizootology of the Republic of Kazakhstan.

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References

Al Dahouk, S., Le Flèche, P., Nöckler, K., Jacques, I., Grayon, M., Scholz, H. C., Tomaso, H., Vergnaud, G., Neubauer, H. 2007. Evaluation of Brucella MLVA typing for human brucellosis. Journal of Microbiological Methods, vol. 69, no. 1, p. 137-145. https://doi.org/10.1016/j.mimet.2006.12.015 DOI: https://doi.org/10.1016/j.mimet.2006.12.015

Alton, G. G., Jones, L. М., Angus, R. D., Verger, J. M. 1988. Techniques for the brucellosis laboratory. Paris, France : INRA, 190 p. ISBN 2738000428.

Ayala, F. J., Kiger, J. A. 1980. Modern Genetics. San Mateo, USA : Benjamin/Cummings, 89 p. ISBN 0805303162.

Borriello, G., Peletto, S., Lucibelli, M. G., Acutis, P. L., Ercolini, D., Galiero, G. 2013. Link between geographical origin and occurrence of Brucella abortus biovars in cow and water buffalo herds. Applied and Environmental Microbiology, vol. 79, no. 3, p. 1039-1043. https://doi.org/10.1128/AEM.02887-12 DOI: https://doi.org/10.1128/AEM.02887-12

Bricker, B. J., Ewalt, D. R. 2005. Evaluation of the HOOF-Print assay for typing Brucella abortus strains isolated from cattle in the United States: results with four performance criteria. BMC Microbiology, vol. 5, p. 37 https://doi.org/10.1186%2F1471-2180-5-37 DOI: https://doi.org/10.1186/1471-2180-5-37

Corbel, M. J. 1997. Brucellosis: an overview. Emerging Infectious Diseases, vol. 3, no. 2, p. 213-221 https://dx.doi.org/10.3201%2Feid0302.970219 DOI: https://doi.org/10.3201/eid0302.970219

Demirev, P. A. 2013. Dried blood spots: Analysis and applications. Analytical Chemistry, vol. 85 no. 2, p. 779-789. https://doi.org/10.1021/ac303205m DOI: https://doi.org/10.1021/ac303205m

Ferreira, A. C., Chambel, L., Tenreiro, T., Cardoso, R., Flor, L., Dias, I. T., Pacheco, T., Garin-Bastuji, B., Le Fléche, P., Vergnaud, G., Tenreiro, R., de Sá, M. I. C. 2012. MLVA16 typing of portuguese human and animal Brucella melitensis and Brucella abortus isolates. PLoS One vol. 7, no. 8, p. e42514. https://doi.org/10.1371/journal.pone.0042514 DOI: https://doi.org/10.1371/journal.pone.0042514

Ficht, T. 2010. Brucella taxonomy and evolution. Future Microbiology, vol. 5, no. 6, p. 859-866. https://doi.org/10.2217/fmb.10.52 DOI: https://doi.org/10.2217/fmb.10.52

Frachetti, M. D., Smith, C. E., Traub, C. M., Williams, T. 2017. Nomadic ecology shaped the highland geography of Asia’s silk roads. Nature, vol. 543, p. 193-198. https://doi.org/10.1038/nature21696 DOI: https://doi.org/10.1038/nature21696

Gandara, B., Merino, A. L., Rogel, M. A., Martinez-Romero, E. 2001. Limited genetic diversity of Brucella spp. Journal of Clinical Microbiology, vol. 39, no. 1, p. 235-240. https://doi.org/10.1128%2FJCM.39.1.235-240.2001 DOI: https://doi.org/10.1128/JCM.39.1.235-240.2001

Garofolo, G., Di Giannatale, E., De Massis, F., Zilli, K., Ancora, M., Cammà, C., Calistri, P., Foster, J. T. 2013. Investigating genetic diversity of Brucella abortus and Brucella melitensis in Italy with MLVA-16. Infection, Genetics and Evolution, vol. 19, p. 59-70. https://doi.org/10.1016/j.meegid.2013.06.021 DOI: https://doi.org/10.1016/j.meegid.2013.06.021

Gershkovich, I. 1968. Genetics. Moscow, USSR : Nauka, 698 p.

Godfroid, J., Scholz, H. C., Barbier, T., Nicolas, C., Wattiau, P., Fretin, D., Whatmore, A. M., Cloeckaert, A., Blasco, J. M., Moriyon, I., Saegerman, C., Muma, J. B., Dahouk, S. Al. Neubauer, H., Letesson, J.-J. 2011. Brucellosis at the animal/ecosystem/human interface at the beginning of the 21st century. Preventive Veterinary Medicine, vol. 102, no. 2, p. 118-131. https://doi.org/10.1016/j.prevetmed.2011.04.007 DOI: https://doi.org/10.1016/j.prevetmed.2011.04.007

Gwida, M., Neubauer, H., Ilhan, Z., Schmoock, G., Melzer, F., Nöckler, K., Janczyk, P., Tomaso, H., Rösler, U., Dahouk, S. Al. 2012. Cross-border molecular tracing of brucellosis in Europe. Comparative Immunology, Microbiology and Infectious Diseases, vol. 35, no. 2, p. 181-185. https://doi.org/10.1016/j.cimid.2011.12.012 DOI: https://doi.org/10.1016/j.cimid.2011.12.012

Kattar, M. M., Jaafar, R. F., Araj, G. F., Le Flèche, P., Matar, G. M., Rached, R. A., Khalife, S., Vergnaud, G. 2008. Evaluation of a multilocus variable-number tandem-repeat analysis scheme for typing human brucella isolates in a region of brucellosis endemicity. Journal of Clinical Microbiology, vol. 46, no. 12, p. 3935-3940. https://doi.org/10.1128/JCM.00464-08 DOI: https://doi.org/10.1128/JCM.00464-08

Le Flèche, P., Jacques, I., Grayon, M., Al Dahouk, S., Bouchon, P., Denoeud, F., Nöckler, K., Neubauer, H., Guilloteau, L. A., Vergnaud, G. 2006. Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiology, vol. 6, no. 1, p. 9. https://doi.org/10.1186/1471-2180-6-9 DOI: https://doi.org/10.1186/1471-2180-6-9

Meyer, M. E. 1990. Evolutionary development and taxonomy of the genus Brucella. In Adams, L. G. Advances in Brucellosis Research. Galveston, USA : Texas A&M University Press, 542 p. ISBN-13 978-0-89096-447-7.

Pappas, G., Panagopoulou, P., Christou, L., Akritidis, N. 2006. Brucella as a biological weapon. Cellular and Molecular Life Sciences, vol. 63, p. 2229-2236. https://doi.org/10.1007/s00018-006-6311-6314 DOI: https://doi.org/10.1007/s00018-006-6311-4

Ridler, A. L., Leyland, M. J., Fenwick, S. G., West, D. M. 2005. Demonstration of polymorphism among Brucella ovis field isolates by pulsed-field gel electrophoresis. Veterinary Microbiology, vol. 108, no. 1-2, p. 69-74. https://doi.org/10.1016/j.vetmic.2005.02.004 DOI: https://doi.org/10.1016/j.vetmic.2005.02.004

Shevtsov, A., Ramanculov, E., Shevtsova, E., Kairzhanova, A., Tarlykov, P., Filipenko, M., Dymova, M., Abisheva, G., Jailbekova, A., Kamalova, D., Chsherbakov, A., Tulegenov, S., Akhmetova, A., Sytnik, I., Karibaev, T., Mukanov, K. 2015. Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16. Infection, Genetics and Evolution, vol. 34, p. 173-180. https://doi.org/10.1016/j.meegid.2015.07.008 DOI: https://doi.org/10.1016/j.meegid.2015.07.008

Scholz, H. C., Vergnaud, G. 2013. Molecular characterisation of Brucella species. Revue Scientifique et Technique, vol. 32, no. 1, p. 149-162. https://doi.org/10.20506/rst.32.1.2189 DOI: https://doi.org/10.20506/rst.32.1.2189

Whatmore, A. M. 2009. Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens. Infection, Genetics and Evolution, vol. 9, no. 6, p. 1168-1184. https://doi.org/10.1016/j.meegid.2009.07.001 DOI: https://doi.org/10.1016/j.meegid.2009.07.001

Whatmore, A. M., Shankster, S. J., Perrett, L. L. Murphy, T. J., Brew, S. D., Thirlwall, R. E., Cutler, S. J., MacMillan, A. P. 2006. Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp. Journal of Clinical Microbiology, vol. 44, no. 6, p. 1982-1993. https://doi.org/10.1128/jcm.02039-05 DOI: https://doi.org/10.1128/JCM.02039-05

Yagupsky, P. 1999. Detection of Brucellae in blood cultures. Journal of Clinical Microbiology, vol. 37, p. 3437-3442. https://doi.org/10.1128/jcm.37.11.3437-3442.1999 DOI: https://doi.org/10.1128/JCM.37.11.3437-3442.1999