Effect of apple cider vinegar on plasma lipids (model experiment in mice)


  • László Bárdos Department of Animal Physiology and Health, Szent István University, 2013 GödöllÅ‘
  • Balázs Bender ImmunoGenes Ltd, 2092 Budakeszi




apple cider vinegar, cholesterol, triglyceride, FRAP, mice


Model experiment was carried out to investigate the effect of apple cider vinegar (ACV) on the blood and liver cholesterol (Ch), triglycerides (TG) and one of a marker of antioxidant status of blood (FRAP) in laboratory mice. Animals consumed a basal mice diet (Control) served as the control group. The same diet was supplemented either 1% cholesterol (Ch) or 1% edible sunflower oil (SFO). All groups were duplicated and their animals were supplied drinking water containing ACV (50 mg l-1)(groups: Control+ACV, Chol+ACV, SFO+ACV).The feeding and drinking was ad libitum for 21 days. At the end of experiment the animals were exterminated. Blood and liver samples were analyzed for total cholesterol (tCh), triglycerides (TG) and ferric reducing antioxidant power (FRAP). The results show that the Ch supplemented group stored higher concentration of tCh in the liver (P<0.01) than SFO treated animals. The cholesterol reserves were less in ACV treated groups. The alterations of plasma tCh showed no significant changes by cholesterol or SFO supplementation and drinking ACV containing water. The concentration of plasma and liver TG remained in the same range in all groups independently by different treatments. Animals of SFO supplemented groups (SFO and SFO+ACV) got more fatten than Control and Ch groups and their liver/body mass ratio (%) decreased (P<0.05). The ACV exerted a decreasing effect on the level of plasma tCh and TG markedly (P<0.05) but only in that group (Control+ACV) which consumed the basal diet. This lowering effect could be demonstrated only in the case of TG in the liver. The groups receiving ACV showed decreasing FRAP values. This means a lower antioxidative capacity of plasma. The ACV can helps in the lowering of plasma lipids (tCh and TG) and can depress their liver storage in the case of normal level of lipid consumption. When the lipid input was elevated this benefit not occurred.


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APRIKIAN, O., LEVRAT-VERNY, M. A., BESSON, C., BUSSEROLLES, J., RÉMÉSY, CH., DEMIGNÉ, CH. 2001. Apple favourably affects parameters of cholesterol metabolism and of anti-oxidative protection in cholesterol-fed rats. In Food Chemistry, vol. 75, 2001, p. 445-452. https://doi.org/10.1016/S0308-8146(01)00235-7 DOI: https://doi.org/10.1016/S0308-8146(01)00235-7

BÁRDOS, L., KISS, ZS. 2000a. The model has proven too (in Hungarian). In Kistermelők Lapja, vol. 44, 2000, p. 15-16.

BÁRDOS, L., KISS, ZS. 2000b. The acid of life (in Hungarian). In Magyar Mezőgazdaság, vol. 55, 2000, p.28.

BENZIE, F. F., STRAIN, J. J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. In Analytical Biochemistry, vol. 239, 1996. p. 70-76. https://doi.org/10.1006/abio.1996.0292 DOI: https://doi.org/10.1006/abio.1996.0292


BEYERS, R. S., JENSEN, L. S. 1993. Reduced plasma-cholesterol and lipoprotein in laying hens without concomitant reduction of egg cholesterol in response to dietary sorbose. In Poultry Sci., vol.72, 1993, p. 88-97. https://doi.org/10.3382/ps.0720088 PMid:8426850 DOI: https://doi.org/10.3382/ps.0720088

CZIRLE, N., BÁRDOS L. 2000. More effective feeding of turkey by using apple cider vinegar (in Hungarian) In Kistermelők Lapja, vol. 45, 2000. p. 20.

FLOCH, J. LEES, M., SLOANE-STANLEY, G. H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. In Journal of Biological Chemistry, vol. 226, 1957. p. 497-509. PMiD:13428781 DOI: https://doi.org/10.1016/S0021-9258(18)64849-5

FURUSE, M. ISHII, T., MIYAGAWA, S., NAKAGAWA, J., SHIMIZU, T., WATANABE, T., OKUMURA, J. I., KIMURA, J. I. 1991. Effect of dietary sorbose on lipid-metabolism in male and female broilers. In Poultry Sci., vol. 70, 1991, p. 95-102. PMid:2017422 DOI: https://doi.org/10.3382/ps.0700095

HELLMISS, M. 1997. Natürlich heilen mit Apfelessig. Südwest Verlag, München.

KONDO, T., KISHI, M., FUSHIMI, T., KAGA, T. 2009. Acetic Acid Upregulates the Expression of Genes for Fatty Acid Oxidation Enzymes in Liver To Suppress Body Fat Accumulation. In Journal of Agricultural and Food Chemistry, vol. 57, 2009, p. 5982-5986. https://doi.org/10.1021/jf900470c PMid:19469536 DOI: https://doi.org/10.1021/jf900470c

LEONTOWICZ, M., GORINSTEIN, S. H., BARTNIKOWSKA, E., LEONTOWICZ, H., KULASEK, G., TRAKHTENBERG, S. 2001. Sugar beet pulp and apple pomace dietary fibers improve lipid metabolism in rats fed cholesterol. In Food Chemistry, vol. 72, 2001, p. 73-78. https://doi.org/10.1016/S0308-8146(00)00207-7 DOI: https://doi.org/10.1016/S0308-8146(00)00207-7

LINDER, M. C. 1991. Nutritional biochemistry and metabolism with clinical applications. Elsevier, New York, NY, p. 74-75.

MARSHALL, W. J. 1988. Clinical chemistry. Glower Medical Publ., London, UK.

MCCOMB, E. A. 1975. Occurrence of L-sorbose in apple-cider vinegar. In Carbohydrate Research, vol. 42, 1975, p. 200-202. https://doi.org/10.1016/S0008-6215(00)84118-X DOI: https://doi.org/10.1016/S0008-6215(00)84118-X

PEARSON, D. A., TAN, C. H., GERMAN, J. B., DAVIS, P. A., GERSHWIN, M. E. 1999. Apple juice inhibits human low density lipoprotein oxidation. In Life Sci., vol. 64, 1999. p. 1913-1920. https://doi.org/10.1016/S0024-3205(99)00137-X PMiD:10353589 DOI: https://doi.org/10.1016/S0024-3205(99)00137-X

RASKIN, I., RIPOLL, C. 2004. Can an apple a day keep the doctor away? In Current Pharmaceutical Design, vol. 10, 2004, p. 3419-3429. https://doi.org/10.2174/1381612043383070 PMid:15544525 DOI: https://doi.org/10.2174/1381612043383070

SAJIKI, J., TAKAHASHI, H., TAKAHASHI, K. 1995. Impact of vinegar acetic acid on hydrolysis and oxidation of lipids in tissues of oyster Crassostrera gigas, at 37 °C. In Journal of Agricultural and Food Chemistry, vol. 1995, p. 1467-1471. https://doi.org/10.1021/jf00054a010 DOI: https://doi.org/10.1021/jf00054a010

SEMBRIES, S., DONGOWSKI, G., MEHRLANDER, K., WILL, F., DIETRICH, H. 2004. Dietary fiber-rich colloids from apple pomace extraction juices do not affect food intake and blood serum lipid levels, but enhance fecal excretion of steroids in rats. In Journal of Nutritional Biochemistry, vol.15, 2004. p. 296-302. https://doi.org/10.1016/j.jnutbio.2003.12.005 PMid:15135154 DOI: https://doi.org/10.1016/j.jnutbio.2003.12.005

TAMURA, Y., FURUSE, M., MATSUDA, S., SHIMIZU, T., OKUMURA, J. 1999. Energy utilization of dietary sorbose in growing rats. In Journal of Agricultural and Food Chemistry, vol. 39, 1999. p. 1623-1626. doi.org/10.1021/jf00009a017 DOI: https://doi.org/10.1021/jf00009a017

VIJAYAKUMAR, C., WOLF-HALL, C. E. 2002. Evaluation of household sanitizers for reducing levels of Escherichia coli on iceberg lettuce. In Journal of Food Protection, vol. 65, 2002, p. 1646-1650. PMid:12380754 DOI: https://doi.org/10.4315/0362-028X-65.10.1646

WILSON, W. O., ABBOTT, U. K., ABPLANALP, H. 1961. Evaluation of coturnix (Japanese quail) as pilot animal for poultry. In Poultry Sci., vol. 40, 1961, p. 651-657. DOI: https://doi.org/10.3382/ps.0400651




How to Cite

Bárdos, L. ., & Bender, B. . (2012). Effect of apple cider vinegar on plasma lipids (model experiment in mice). Potravinarstvo Slovak Journal of Food Sciences, 6(1), 1–4. https://doi.org/10.5219/156