Comparative study of weaning pigs' muscle proteins using two-dimensional electrophoresis

Authors

  • Anastasia Akhremko . M. Gorbatov Federal Research Center for Food Systems of RAS, Experimental-clinical research laboratory of bioactive substances of animal origin, Talalikhina st., 26, 109316, Moscow, Russia, Tel.: +79152379497 https://orcid.org/0000-0002-0211-8171
  • Liliya Fedulova V. M. Gorbatov Federal Research Center for Food Systems of RAS, Experimental-clinical research laboratory of bioactive substances of animal origin, Talalikhina st., 26, 109316, Moscow, Russia, Tel.: +74956769211 https://orcid.org/0000-0003-3573-930X

DOI:

https://doi.org/10.5219/1449

Keywords:

2-DE, muscle, protein, two-dimensional electrophoresis, pig, piglet

Abstract

The proteostasis system of animals, including various types of protein modification during the growth stage, leads to an almost incomprehensible number of possible forms of protein, and each can regulate numerous functions. In the presented work, the composition of muscle tissue protein from different portions of piglets was studied to understand the main muscle protein formation. Comparative analysis of weaned piglets' main muscle protein from l. dorsi, biceps femoris, and brachiocephalicus were analyzed using two-dimensional electrophoresis. Changes in the staining intensity of protein fractions inherent in different muscles were revealed. As part of this work, candidate groups of pig muscle proteins have been selected. Eleven protein spots were revealed for the longest muscle of the back, and seven for the biceps; the muscles of the neck are characterized by indicators of low protein fraction volume. Among the proteins found, myosin light chains, phosphoglycerate mutase, troponins, and adenylate kinase is most likely present. The obtained results of protein identification in muscle tissues, obtained during the intensive growth period, will allow a more detailed understanding of protein regulation, function, and interactions in complex biological systems, which will subsequently be significantly important for biomonitoring health and predicting farm animals productivity.

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Published

2021-01-28

How to Cite

Akhremko, A., & Fedulova, L. (2021). Comparative study of weaning pigs’ muscle proteins using two-dimensional electrophoresis. Potravinarstvo Slovak Journal of Food Sciences, 15, 52–57. https://doi.org/10.5219/1449