Investigating chemical changes during Snake fruit and Black tea Kombucha fermentation and the associated immunomodulatory activity in Salmonella typhi-infected mice
DOI:
https://doi.org/10.5219/1416Keywords:
kombucha, snake fruit, black tea, immunomodulator, Salmonella typhiAbstract
This study uncovered the chemical changes during kombucha's fermentation process and revealed the associated immunomodulatory activity in Salmonella typhi-infected mice. The snake fruit juice and black tea extract were processed into kombucha (a beverage known for its health benefits) by fermentation with SCOBY culture at room temperature for 14 days. Snake fruit kombucha showed high changes in fermentation parameters (total acidity, pH, and total sugar), as well as bioactive compounds and antioxidant activity. Salmonella typhi demonstrated a reduction in the population of CD8+TNFα+ and CD4+IFNγ+ of infected experimental animals. Both snake fruit kombucha and black tea kombucha have the potential to be utilized as an immunomodulator to circumvent unstable conditions of the immune system caused by Salmonella typhi. Black tea kombucha and snake fruit kombucha cancan raise the production of CD8+TNFα+ and CD4+IFNγ+ in mice infected with Salmonella typhi. In the group of normal mice, black tea and snake fruit kombucha were able to lower down the production of CD8+TNFα+, which is a potent mechanism to modulate the immune system. Further research is required to highlight the mechanism and role of black tea kombucha and snake fruit kombucha in the immune response that modulates and treats infection by Salmonella typhi.
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Abbas, A. K., Lichtman, A. H., Pilai, S. 2007. Cellular and molecular immunology. 6th ed. Philadelphia, USA : Elsevier. ISBN-13 978-8131210345.
Amit, A., Dikhit, M. R., Mahantesh, V., Chaudhary, R., Singh, A. K., Singh, A., Singh, S. K., Das, V. N. R., Pandey, K., Ali, V., Narayan, S., Sahoo, G. C., Das, P., Bimal, S. 2017. Immunomodulation mediated through Leishmania donovani protein disulfide isomerase by eliciting D*+ T=cell in cured visceral lesihmaniasis subjects and identification of its possible HLA class-1 restricted T-cell epitopes. Journal of Biomolecular Structure and Dynamics, vol. 35 no. 1, p. 128-140. https://doi.org/10.1080/07391102.2015.1134349 DOI: https://doi.org/10.1080/07391102.2015.1134349
AOAC. 1995. Official methods of analysis of AOAC international. 16th ed. Washington DC, USA : The Association of Official Analytical Chemists. ISBN-10 0935584544.
Apriyadi, T. E. 2017. Potensi kombucha salak Suwaru sebagai agen terapi hiperglikemia pada model tikus wistar diabetes mellitus (Potency of Suwaru snake fruit kombucha as an hyperglycemia therapeutic agent in a diabetes mellitus wistar mice model : dissertation theses. Malang, Indonesia : Universitas Brawijaya. (in Indonesian)
Aralas, S., Mohamed, M., Abu Bakar, M. F. 2009. Antioxidant properties of selected salak (Salacca zalacca) varieties in Sabah, Malaysia. Nutrition & Food Science, vol. 39, no. 3, p. 243-250. https://doi.org/10.1108/00346650910957492 DOI: https://doi.org/10.1108/00346650910957492
Atanassova, M., Georgieva, S., Ivancheva, K. 2011. Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs. Journal of the University of Chemical Technology and Metallurgy, vol. 46, no. 1, p. 81-88.
Bhattacharya, S., Gachhui, R., Sil, P. C. 2013. Effect of Kombucha, a fermented black tea in attenuating oxidative stress mediated tissue damage in alloxan induced diabetic rats. Food and Chemical Toxicology, vol. 60, p. 328-340. https://doi.org/10.1016/j.fct.2013.07.051 DOI: https://doi.org/10.1016/j.fct.2013.07.051
Bhuiyan, M. S., Sayeed, M. A., Khanam, F., Leung, D. T., Bhuiyan, T. R., Sheikh, A., Salma, U., LaRocque, R. C., Harris, J. B., Pacek, M., Calderwood, S. B., LaBaer, J., Ryan, E. T., Qadri, F., Charles, R. C. 2014. Cellular and cytokine responses to Salmonella enterica serotype typhi proteins in patients with typhoid fever in Bangladesh. The American Journal of Tropical Medicine and Hygiene, vol. 90, no. 6, p. 1024-1030. https://doi.org/10.4269/ajtmh.13-0261 DOI: https://doi.org/10.4269/ajtmh.13-0261
Buttler, T., Girard, E. 1993. Comparative efficacies of azithromycin and ciprofloxacin against experimental Salmonella typhimurium infection in mice. Journal of Antimicrobial Chemotheraphy, vol. 31, no. 2, p. 313-319. https://doi.org/10.1093/jac/31.2.313 DOI: https://doi.org/10.1093/jac/31.2.313
Clinton, C. 2009. Plant tannins a novel approach to the treatment of ulcerative colitis. Journal of Natural Medicines, vol. 2, p. 1-3.
Crump, J. A. 2019. Progress in typhoid fever epidemiology. Clinical Infectious Diseases, vol. 68, p. S4-S9. https://doi.org/10.1093/cid/ciy846 DOI: https://doi.org/10.1093/cid/ciy846
Damayanti, E., Suparjana, T. B. 2007. Efek penghambatan beberapa fraksi ekstrak buah mengkudu terhadap Shigella dysenteriae (Inhibition effect of several fractions of noni fruit extract against Shigella Shigella). Prosiding Seminar Nasional Teknik Kimia Kejuangan. Fakultas Biologi Universitas Jenderal Soedirman, Purwokerto. (in Indonesian)
Dufresne, C., Farnworth, E. 2000. Tea, kombucha, and health: a review. Food Research International, vol. 33, p. 409-421. https://doi.org/10.1016/S0963-9969(00)00067-3 DOI: https://doi.org/10.1016/S0963-9969(00)00067-3
Essawet, N. A., Cvetkovic, D., Velicanski, A., Canadanovic-Brunet, J., Vulic, J., Maksimovic, V., Markov, S. 2015. Polyphenols and antioxidant activities of Kombucha beverage enriched with coffeeberry extract. Chemical Industry & Chemical Engineering Quarterly, vol. 21, no. 3, p. 399-409. https://doi.org/10.2298/CICEQ140528042E DOI: https://doi.org/10.2298/CICEQ140528042E
Fuller, R. 1989. Probiotics in man and animals. Journal of Applied Bacteriology, vol. 66, p. 365-378. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x DOI: https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
Jayabalan, R., Malbasa, R. V., Loncar, E. S., Vitas, J. S., Sathishkumar, M. A. 2014. Review on Kombucha tea – microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Comprehensive Reviews in Food Science and Food Safety, vol. 13, p. 538-550. https://doi.org/10.1111/1541-4337.12073 DOI: https://doi.org/10.1111/1541-4337.12073
Jayabalan, R., Marimuthu, S., Swaminathan, K. 2007. Changes in content of organic acids and tea polyphenols during kombucha tea fermentation. Food Chemistry, vol. 102, p. 392-398. https://doi.org/10.1016/j.foodchem.2006.05.032 DOI: https://doi.org/10.1016/j.foodchem.2006.05.032
Keuter, M. 1998. Experimental studies on pathogenesis of Salmonella infection : dissertation theses. Netherland : Katholiek Universiteit Nijmegen.
Khan, M. I., Soofi, S. B., Ochiai, R. L., Khan, M. J., Sahito, S. M. 2012. Epidemiology, clinical presentation and patterns of drug resistance of Salmonella typhi in Karachi, Pakistan. Journal of Infection in Developing Coutries, vol. 6, p. 704-714. https://doi.org/10.3855/jidc.1967 DOI: https://doi.org/10.3855/jidc.1967
Loresta, S., Murwani, S., Trisunuwati, P. 2012. Efek ekstrak etanol daun kelor (Moringa oleifera) terhadap pembentukan biofilm Staphylococcus aureus secara in vitro (Effect of Moringa oleifera leaf ethanol extract towards biofilm formation by Staphylococcus aureus, in vitro) : dissertation theses. Malang, Indonesia : Universitas Brawijaya. (in Indonesian)
Lyu, S. Y., Park, W. B. 2005. Production of cytokine and NO by RAW 264.7 macrophages and PBMC in vitro incubation with flavonoids. Archives of Pharmacal Research, vol. 28, p. 573-581. https://doi.org/10.1007/bf02977761 DOI: https://doi.org/10.1007/BF02977761
Malbasa, R. V., Loncar, E. S., Vitas, J. S., Canadanovic-Brunet, J. M. 2011. Influence of starter cultures on the antioxidant activity of kombucha beverage. Food Chemistry, vol. 127, p. 1727-1731. https://doi.org/10.1016/j.foodchem.2011.02.048 DOI: https://doi.org/10.1016/j.foodchem.2011.02.048
Nurhalimah, H., Wijayanti, N., Widyaningsih, T. 2015. Efek antidiare ekstrak daun beluntas (Pluchea indica L.) terhadap mencit jantan yang diinduksi bakteri Salmonella thypimurium (Antidiarrheal effects of beluntas leaf extract (Pluchea indica L.) against male mice induced by Salmonella typhimurium). Jurnal Pangan Agroindustri, vol. 3, no. 3, p. 1083-1094. (in Indonesian)
Oppenheim, J. J., Ruscetti, F. W. 2003. Cytokines. In: Parslow, T.G. Et al. Medical immunology. 10th ed. Boston : McGraw-Hill. ISBN-13 978-0838563007.
Pinsirodom, P., Rungcharoen, J., Liumminful, A. 2010. Quality of commercial wine vinegars evaluated on the basis of total polyphenol content and antioxidant properties. Asian Journal of Food and Agro-Industry, vol. 3, no. 4, p. 389-397.
Ramadhan, M. F., Mahfudh, N., Sulistyani, N. 2020. Isolation and identification of active compound from bengle rhizome (Zingiber cassumunar Roxb) as a stimulant in phagocytosis by macrophages. Potravinarstvo Slovak Journal of Food Sciences, vol. 14, no. 1, p. 328-335. https://doi.org/10.5219/1238 DOI: https://doi.org/10.5219/1238
Saini, R. K., Sivanesan, I., Keum, Y. 2016. Phytochemicals of Moringa oleifera: a review of their nutritional, therapeutic and industrial significance. 3 Biotech, vol. 6, p. 203. https://doi.org/10.1007/s13205-016-0526-3 DOI: https://doi.org/10.1007/s13205-016-0526-3
Samuel, C. E. 2001. Antiviral actions of interferons. Clinical Microbiology Reviews, vol. 14, no. 4, p. 778-809. https://doi.org/10.1128/cmr.14.4.778-809.2001 DOI: https://doi.org/10.1128/CMR.14.4.778-809.2001
Sheikh, A., Khanam, F., Sayeed, M. A., Rahman, T., Pacek, M. 2011. Interferon-γ and proliferation resonses to Salmonella enterica serotype typhi proteins in patients with S. typhi bacteremia in Dhaka, Bangladesh. PLOS Neglected Tropical Diseases, vol. 5, p. 6. https://doi.org/10.1371/journal.pntd.0001193 DOI: https://doi.org/10.1371/journal.pntd.0001193
Sreeramulu, G., Zhu, Y., Knol, W. 2000. Kombucha fermentation and its antimicrobial activity. Journal of Agricultural and Food Chemistry, vol. 48, p. 2589-2594. https://doi.org/10.1021/jf991333m DOI: https://doi.org/10.1021/jf991333m
Suica-Bunghez, I. R., Teodorescu, S., Dulama, I. D., Voinea, O. C., Simionescu, S., Ion, R. M. 2016. Antioxidant activity and phytochemical compounds of snake fruit (Salacca zalacca). IOP Conf. Series: Materials Science and Engineering, vol. 133, p. 1-8. https://doi.org/10.1088/1757-899X/133/1/012051 DOI: https://doi.org/10.1088/1757-899X/133/1/012051
Sulistiani, R. P., Rahayuningsih, H. M. 2015. Pengaruh ekstrak lompong (Colocasia esculenta L Schoot) mentah terhadap aktivitas fagositosis dan kadar NO (Nitrit Oksida) mencit Balb/C sebelum dan sesudan terinfeksi Listeria monocytogenes (Effect of raw lompong (Colocasia esculenta L Schoot) on the phagocytosis and NO (Nitrite Oxide) level in Balb/C mice before and after infected by Listeria monocytogenes) : dissertation theses. Semarang, Indonesia : Universitas Diponegoro. https://doi.org/10.14710/jnc.v4i4.10118 DOI: https://doi.org/10.14710/jnc.v4i4.10118
Supapvanich, S., Megia, R., Ding, P. 2011. Salak (Salacca zalacca (Gaertner) Voss.). In Yahia, E.M. Postharvest biology and technology of tropical and subtropical fruits (Volume 4: mangosteen to white sapote). Cambridge, UK : Woodhead Publishing Limited. ISBN-13 978-0857090904. https://doi.org/10.1533/9780857092618.334 DOI: https://doi.org/10.1533/9780857092618.334
Thung, T. Y., Radu, S., Mahyudin, N. A., Rukayadi, Y., Zakaria, Z., Mazlan, N., Tan, B. H., Lee, E., Yeoh, S. L., Chin, Y. Z., Tan, C. W., Kuan, C. H., Basri, D. F., Wan Mohamed Radzi, C. W. J. 2017. Prevalence, virulence genes and antimicrobial resistance profiles of Salmonella serovars from retail beef in Selangor, Malaysia. Frontiers in Microbiology, vol. 8, p. 2697. https://doi.org/10.3389/fmicb.2017.02697 DOI: https://doi.org/10.3389/fmicb.2017.02697
Ukhrowi, U. 2011. Pengaruh pemberian ekstrak etanol umbi bidara upas (Merremia mammosa) terhadap fagositosis makrofag dan produksi nitrit oksida (NO) makrofag – studi pada mencit balb/c yang diinfeksi Salmonella typhimurium (The effect of ethanolic extract of bidara upas tuber (Merremia mammosa) on phagocytosis macrophage and nitric oxide (NO) macrophage production – study in balb/c mice infected by Salmonella typhimurium) : dissertation theses. Semarang, Indonesia : Universitas Diponegoro. (in Indonesian)
Yang, J., Paulino, R., Janke-Stedronsky, S. 2007. Free-radical-scavenging activity and total phenols of noni (Moringa citrifolia L.) juice and powder in processing and storage. Food Chemistry, vol. 102, p. 302-308. https://doi.org/10.1016/j.foodchem.2006.05.020 DOI: https://doi.org/10.1016/j.foodchem.2006.05.020
Zubaidah, E., Afgani, C. A., Kalsum, U., Srianta, I., Blanc, P. J. 2018c. Comparison of in vivo antidiabetes activity of snake fruit Kombucha, black tea Kombucha and metformin. Biocatalysis and Agricultural Biotechnology, vol. 17, p. 465-469. https://doi.org/10.1016/j.bcab.2018.12.026 DOI: https://doi.org/10.1016/j.bcab.2018.12.026
Zubaidah, E., Apriyadi, T. E., Kalsum, U., Widyastuti, E., Estiasih, T., Srianta, I., Blanc, P. J. 2018b. In vivo evaluation of snake fruit Kombucha as hyperglycemia therapeutic agent. International Food Research Journal, vol. 25, no. 1, p. 453-457.
Zubaidah, E., Dewantari, F. J., Novitasari, F. R., Srianta, I., Blanc, P. J. 2018a. Potential of snake fruit (Salacca zalacca (Gaerth.) Voss) for the development of a beverage through fermentation with the Kombucha consortium. Biocatalysis and Agricultural Biotechnology, vol. 13, p. 198-203. https://doi.org/10.1016/j.bcab.2017.12.012 DOI: https://doi.org/10.1016/j.bcab.2017.12.012
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