Impact of humic acid as an organic additive on the milk parameters and occurrence of mastitis in dairy cows

Authors

  • František Zigo University of Veterinary Medicine and Pharmacy, Department of Animal Husbandry, Komenského 73, 041 81 Košice, Slovakia, Tel.: +421908689722 https://orcid.org/0000-0002-2791-166X
  • Milan Vasiľ University of Veterinary Medicine and Pharmacy, Department of Animal Husbandry, UVLF, Komenského 73, 041 81 Košice, Slovakia, Tel.: +421905385671 https://orcid.org/0000-0002-3607-818X
  • Zuzana Farkašová University of Veterinary Medicine and Pharmacy, Department of Animal Husbandry, UVLF, Komenského 73 041 81, Košice, Slovakia, Tel.: +421905201646 https://orcid.org/0000-0003-1953-1840
  • Silvia Ondrašovičová University of Veterinary Medicine and Pharmacy, Department of Physiology, UVLF, Komenského 73, 041 81 Košice, Slovakia, Tel.: +421915984702
  • Martina Zigová University of Veterinary Medicine and Pharmacy, Department of Pharmacology, Pavol Jozef Šafárik University of Košice, Šrobárova 1014/2, 040 01 Košice, Slovakia, Tel.: +421908689722
  • Jana Maľová University of Veterinary Medicine and Pharmacy, Department of Milk Hygiene, UVLF, Komenského 73 041 81, Košice, Slovakia, Tel.: +421915986955
  • Jana Výrostková University of Veterinary Medicine and Pharmacy, Department of Milk Hygiene, UVLF, Komenského 73 041 81, Košice, Slovakia, Tel.: +421908360406 https://orcid.org/0000-0002-3117-8915
  • Jolanta Bujok Wrocław University of Environmental and Life Sciences, Department of Animal Physiology and Biostructure Norwida 31, 50-375 Wroclaw, Poland, Tel.: +48509735696
  • Ewa Pecka-Kiełb Wrocław University of Environmental and Life Sciences, Department of Animal Physiology and Biostructure, Norwida 31, 50-375 Wroclaw, Poland. Tel.: +48506227929 https://orcid.org/0000-0002-8703-8268

DOI:

https://doi.org/10.5219/1340

Keywords:

dairy cows, supplementation, humid acid, mastitis, milk urea

Abstract

Given growing concerns about the use of antibiotics in the treatment of animals, identifying organic alternatives as feed additives to improve animal health and the development of immune responses has become of interest in dairy farming. Humic acids (HA) seem to be a suitable alternative with a favorable impact on the health and production parameters of animals. This study aimed to determine the effects of an HA supplemented diet on milk parameters as well as the effects on somatic cell count (SCC) and the occurrence of mastitis in dairy cows during the peripartum period. Twenty dairy cows in the last stage of pregnancy were selected from a herd of 140 cows. The selected cows were randomly divided into two groups: control (C) and experimental (E). The two groups were fed the same feed mixture and group E was additionally supplemented with HA at a total dose of 100 g per cow per day during the last 50 days of pregnancy. The milk parameters (dry matter, lactose, fat, crude protein, casein and milk urea) and SCC of every cow, and the presence of mastitis, were checked on days 10 and 30 during the first month of lactation. The results of the study show that dietary supplementation with HA significantly reduced the milk urea (MU) content and SCC on the 10th day after calving but did not affect the other milk compositions. In addition to the decreased MU and SCC, the number of positive quarters detected by the California Mastitis Test was reduced by 20.0% and the occurrence of mastitis caused by coagulase-negative staphylococci (CNS). Based on the obtained results we can conclude that the addition of HA stabilizes the nutrient digestion, as was confirmed by a reduced MU content in the supplemented group. Their indirect beneficial effects improved the development of immune responses, resulting in decreased SCC and the occurrence of mastitis caused by CNS.

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References

AOAC. 2012. Official methods of analysis. 19th ed. ARLINGTON, USA : Association of Official Analytical Chemists.

Dabovich, L. A., Hulbert, L., Rudine, A., Kim, S., McGlone, J. J. 2003. Evaluation of nutriceutical effects on pig immunity: Effects of Promox, 2003. Southern Section ASAS meeting. Pork Industry Institute, Department of Animal and Food Science, Texas Tech University, Lubbock, TX 79409.

Degirmencioglu, T. 2014. Using humic acid in diets for dairy goats. Animal Science Papers and Reports, vol. 32, no. 1, p. 25-32.

FASS. 2010. Guide for the Care and Use of Agricultural Animals in Research and Teaching. 3rd ed. CHAMPAIGN, USA : The Federation of Animal Science Societies.

Galip, N., Polat, U., Biricik, H. 2010. Effects of supplemental humic acid on ruminal fermentation and blood variables in rams. Italian Journal of Animal Science, vol. 9, no. 4, p. 390-393, https://doi.org/10.4081/ijas.2010.e74 DOI: https://doi.org/10.4081/ijas.2010.e74

Idriss, S. E. A. B., Tančin, V., Foltýs, V., Kirchnerová, K., Tančinová, D., Vršková, M. 2013. Relationship between mastitis causative pathogens and somatic cell count in milk of dairy cows. Potravinarstvo, vol. 7, no. 1, p. 207-212. https://doi.org/10.5219/304 DOI: https://doi.org/10.5219/304

Islam, K. M. S., Schumacher, A., Gropp, J. M. 2005. Humic acid substances in animal agriculture. Pakistan Journal of Nutrition, vol. 4, no. 3, p. 126-134. https://doi.org/10.3923/pjn.2005.126.134 DOI: https://doi.org/10.3923/pjn.2005.126.134

Jackson, P. G. G., Cockroft, P. D. 2002. Clinical Examination of Farm Animals. Oxford, UK : Blackwell Science Ltd, Wiley-Blackwell, 303 p. ISBN 0-632-05706-8.

Jaďuttová, I., Marcinčáková, D., Bartkovský, M., Semjon, B., Harčárová, M., Nagyová, A., Váczi, P., Marcinčák, S. 2019. The effect of dietary humic substances on the fattening performance, carcass yield, blood biochemistry parameters and bone mineral profile of broiler chickens. Acta veterinaria Brno, vol. 88, no. 3, p. 307-313. https://doi.org/10.2754/avb201988030307 DOI: https://doi.org/10.2754/avb201988030307

Malinowski, E., Lassa, H., Kłlossowska, A., Smulski, S., Markiewicz, H., Kaczmarowski, M. 2006. Etiological agents of dairy cows’ mastitis in western part of Poland. Polish Journal of Veterinary Sciences, vol. 9, no. 3, p. 191-194.

Marcinčáková, D., Mačanga, J., Nagy, J., Marcinčák, S. Popelka, P. Vašková, J., Jaďuttová, I., Mellen, M. 2015. Effect of supplementation of the diet with humic acids on growth performance and carcass yield of broilers. Folia Veterinaria, vol. 59, no. 3, p. 165-168. Available at: http://www.uvlf.sk/document/folia-veterinaria-volume-59-issue-3.pdf

Mudroňová, D., Karaffová, V., Pešulová, T., Koščová, J., Cingeľová Maruščáková, I., Bartkovský, M., Marcinčáková, D., Ševčíková, Z., Marcinčák, S. 2020. The effect of humic substances on gut microbiota and immune response of broilers. Food and Agricultural Immunology, vol. 31, no. 1, p. 137-149. https://doi.org/10.1080/09540105.2019.1707780 DOI: https://doi.org/10.1080/09540105.2019.1707780

National mastitis council. 2001. National Mastitis Council Recommended Mastitis Control Program. Natl. Mastitis Counc. Ann. Mtg. Proc., Reno, NV. Natl. Mastitis Counc., Inc., Verona, WI. 408 p. ISBN 978-0-309-06997-7. https://doi.org/10.17226/9825 DOI: https://doi.org/10.17226/9825

NRC. 2001. Nutrient Requirements of Dairy Cattle. 7th ed. WASHINGTON, USA : The National Academies Press, National Research Council. 381 p.

Paape, M. J., Wiggens, G. R., Bannerman, D. D., Thomas, D. L., Sanders, A. H., Contreras, A., Moroni, P., Miller, R. H. 2007. Monitoring goat and sheep milk somatic cell counts. Small Ruminant Research, vol. 68, no. 1-2, p. 114-125. https://doi.org/10.1016/j.smallrumres.2006.09.014 DOI: https://doi.org/10.1016/j.smallrumres.2006.09.014

Pecka, E., Dobrzański, Z., Zachwieja, A., Szulc, T., Czyż, K. 2012. Studies of composition and major protein level in milk and colostrum of mares. Animal Science Journal, vol. 83, no. 2, p. 162-68. https://doi.org/10.1111/j.1740-0929.2011.00930.x DOI: https://doi.org/10.1111/j.1740-0929.2011.00930.x

Potůčková, M., Kouřimská, L. 2017. Effect of humates in diet of dairy cows on the raw milk main components. Potravinarstvo Slovak Journal of Food Sciences, vol. 11, no.1, p. 558-563. https://doi.org/10.5219/748 DOI: https://doi.org/10.5219/748

Pyörälä, S., Taponen, S. 2009. Coagulase-negative staphylococci - Emerging mastitis pathogens. Veterinary Microbiology, vol. 134, no. 1-2, p. 3-8. https://doi.org/10.1016/j.vetmic.2008.09.015 DOI: https://doi.org/10.1016/j.vetmic.2008.09.015

Semjon, B., Marcinčáková, D., Koréneková, B., Bartkovský, M., Nagy, J., Turek, P., Marcinčák, S. 2020. Multiple factorial analysis of physicochemical and organoleptic properties of breast and thigh meat of broilers fed a diet supplemented with humic substances. Poultry Science, vol. 99, no. 3, p. 1750-1760. https://doi.org/10.1016/j.psj.2019.11.012 DOI: https://doi.org/10.1016/j.psj.2019.11.012

Sharma, N., Singh, N. K., Bhadwal, M. S. 2011. Somatic cells (Physiological aspects). Asian-Australasian Journal of Animal Sciences, vol. 24, no. 3, p. 429-438. https://doi.org/10.5713/ajas.2011.10233 DOI: https://doi.org/10.5713/ajas.2011.10233

Taponen, S., Simojoki, H., Haveri, M., Larsen, H. D., Pyőrälä, S. 2006. Clinical characteristics and persistence of bovine mastitis caused by different species of coagulase-negative staphylococci identified with API or AFLP. Veterinary Microbiology, vol. 115, no. 13, p. 199-207. https://doi.org/10.1016/j.vetmic.2006.02.001 DOI: https://doi.org/10.1016/j.vetmic.2006.02.001

Thomassen, B. P. H., Faust, R. H. 2000. The use of a processed humic acid product as a feed supplement in dairy production in the Netherlands. The world grows organic international scientific conference, August, Basle, p 339. Available at: https://static1.squarespace.com/static/5b19a9168f5130fd46db6864/t/5ce7f668f9619a3f9b850321/1558705768522/ifoam_dairy-humic_.pdf

Trckova, M., Matlova, L., Hudcova, H., Faldyna, M., Zraly, Z., Dvorska, L., Beran, V., Pavlik, I. 2005. Peat as a feed supplement for animals: a literature review. Veterinari Medicina, vol. 50, p. 361-377. https://doi.org/10.17221/5635-VETMED DOI: https://doi.org/10.17221/5635-VETMED

Van Soest, P. J. 1994. Nutritional Ecology of the Ruminant. 2nd Edition. Ithaca, NY, USA : Cornell University Press. 408 p. ISBN 978-1-501-73235-5.

Xiaowang, X., Shaohua, S., Lixia, H. 2010. Study on the effect of biochemical fulvic acid on somatic cell count and milk performance of dairy cows. Journal of China dairy cattle, vol. 5, 7 p.

Zadoks, R. N., Middleton, J. R., Mcdougall, S., Katholm, J., Schukken, Y. H. 2011. Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans. Journal of Mammary Gland Biology and Neoplasia, vol. 16, p. 357-372. https://doi.org/10.1007/s10911-011-9236-y DOI: https://doi.org/10.1007/s10911-011-9236-y

Zajác, P., Tomáška, M., Murárová, A., Čapla, J., Čurlej, J. 2012. Quality and safety of raw cow’s milk in Slovakia in 2011. Potravinarstvo, vol. 6, no. 2, p. 64-73. https://doi.org/10.5219/189 DOI: https://doi.org/10.5219/189

Zigo, F., Farkašová, Z., Elečko, J., Lapin, M., Chripková, M., Czerski, A. 2014. Effect of parenteral administration of selenium and vitamin e on health status of mammary gland and on selected antioxidant indexes in blood of dairy cows. Polish Journal of Veterinary Sciences, vol. 17, no 2, p. 217-223. https://doi.org/10.2478/pjvs-2014-0031 DOI: https://doi.org/10.2478/pjvs-2014-0031

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Published

2020-06-28

How to Cite

Zigo, F., Vasiľ, M., Farkašová, Z., Ondrašovičová, S., Zigová, M., Maľová, J., Výrostková, J., Bujok, J., & Pecka-Kiełb, E. (2020). Impact of humic acid as an organic additive on the milk parameters and occurrence of mastitis in dairy cows. Potravinarstvo Slovak Journal of Food Sciences, 14, 358–364. https://doi.org/10.5219/1340

Issue

Vol. 14 (2020): Potravinarstvo Slovak Journal of Food Sciences

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Articles

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This license permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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